Reviewed, UniProtKB/Swiss-Prot P55907 (FER1_SULTO)

Names and origin

Protein names

Recommended name:
    Zinc-containing ferredoxin-1

Gene names

Name:	zfx1
Synonyms:	zfx
Ordered Locus Names:	ST0163

Organism

Sulfolobus tokodaii [Complete proteome] [HAMAP]

Taxonomic identifier

111955 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Cofactor	Binds 1 3Fe-4S cluster. Binds 1 4Fe-4S cluster. Binds 1 zinc ion.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.
biophysicochemical properties	Redox potential: E0 is -280 mV for the 3Fe-4S and -530 mV for the 4Fe-4S clusters.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding Zinc
PTM	Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 104

103

Zinc-containing ferredoxin-1

PRO_0000159180

Regions

Domain

38 – 66

29

4Fe-4S ferredoxin-type 1

Domain

75 – 104

30

4Fe-4S ferredoxin-type 2

Region

2 – 37

36

N-terminal extension

Sites

Metal binding

17

1

Zinc

Metal binding

20

1

Zinc

Metal binding

35

1

Zinc

Metal binding

46

1

Iron-sulfur 1 (3Fe-4S)

Metal binding

52

1

Iron-sulfur 1 (3Fe-4S)

Metal binding

56

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

77

1

Zinc

Metal binding

84

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

87

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

90

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

94

1

Iron-sulfur 1 (3Fe-4S)

Amino acid modifications

Modified residue

30

1

N6-methyllysine

Experimental info

Sequence conflict

26

1

V → L AA sequence Ref.3

Sequence conflict

54

1

N → T in BAA22587. Ref.1

Secondary structure

1

.

.

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.

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.

.

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104

Helix Strand Turn

Details...

Helix

7 – 10

4

Beta strand

13 – 17

5

Beta strand

20 – 24

5

Turn

28 – 30

3

Beta strand

37 – 42

6

Turn

43 – 45

3

Helix

51 – 55

5

Beta strand

62 – 65

4

Beta strand

73 – 77

5

Helix

81 – 83

3

Helix

89 – 93

5

Beta strand

99 – 101

3

Sequences

	Sequence		Length	Mass (Da)
	P55907-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 04A9E52F5EDA4F4A	FASTA	104	11,134
	10 20 30 40 50 60 MGIDPNYRTN RQVVGEHSGH KVYGPVEPPK VLGIHGTIVG VDFDLCIADG SCINACPVNV 70 80 90 100 FQWYDTPGHP ASEKKADPVN EQACIFCMAC VNVCPVAAID VKPP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequencing, and heterologous expression of a novel zinc-containing ferredoxin gene from a thermoacidophilic Archaeon Sulfolobus sp. strain 7." Wakagi T., Fujii T., Oshima T. Biochem. Biophys. Res. Commun. 225:489-493(1996) [PubMed: 8753789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
[2]	"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed: 11572479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
[3]	"Alternative form of the dicluster ferredoxin from the thermoacidophilic archaeon, Sulfolobus sp. strain 7." Iwasaki T., Fujii T., Wakagi T., Oshima T. Biochem. Biophys. Res. Commun. 206:563-569(1995) [PubMed: 7826373] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
[4]	"Functional and evolutionary implications of a [3Fe-4S] cluster of the dicluster-type ferredoxin from the thermoacidophilic archaeon, Sulfolobus sp. strain 7." Iwasaki T., Wakagi T., Isogai Y., Tanaka K., Iizuka T., Oshima T. J. Biol. Chem. 269:29444-29450(1994) [PubMed: 7961925] [Abstract] Cited for: CHARACTERIZATION. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
[5]	"The crystal structure of zinc-containing ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7." Fujii T., Hata Y., Oozeki M., Moriyama H., Wakagi T., Tanaka N., Oshima T. Biochemistry 36:1505-1513(1997) [PubMed: 9063899] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D78179 Genomic DNA. Translation: BAA22587.1.
BA000023 Genomic DNA. Translation: BAB65120.1.

PIR

JC4907.

RefSeq

NP_376011.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XER	X-ray	2.00	A	2-104	[»]

ModBase

Search...

Genome annotation databases

GeneID

1458046.

GenomeReviews

Gene locus ST0163 in contig BA000023_GR.

KEGG

sto:ST0163.

NMPDR

fig|273063.1.peg.179.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P55907.

OMA

P55907. DICIADG.

Family and domain databases

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR009157. Fdx_Zn_bd.
[Graphical view]

Pfam

PF00037. Fer4. 2 hits.
[Graphical view]

PIRSF

PIRSF000068. Zn_Fdx_Sulfol. 1 hit.

PROSITE

PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FER1_SULTO

Accession

Primary (citable) accession number: P55907
Secondary accession number(s): O32423

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)