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P55902 (SIX1_BUTOC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-insect toxin BotIT1
OrganismButhus occitanus tunetanus (Common European scorpion)
Taxonomic identifier6871 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeButhus

Protein attributes

Sequence length65 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This contractive toxin is highly toxic to insects and barely toxic to mammals.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Toxic dose

LD50 is 50 µg/kg in mouse by intracerebroventricular injection and 600 ng/g in Blattella germanica. Ref.1

Sequence similarities

Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.

Mass spectrometry

Molecular mass is 7334.93±0.23 Da from positions 1 - 65. Determined by ESI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6565Alpha-insect toxin BotIT1
PRO_0000066713

Amino acid modifications

Disulfide bond12 ↔ 63 By similarity
Disulfide bond16 ↔ 36 By similarity
Disulfide bond22 ↔ 46 By similarity
Disulfide bond26 ↔ 48 By similarity

Sequences

Sequence LengthMass (Da)Tools
P55902 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 93A1371D877D7123

FASTA657,343
        10         20         30         40         50         60 
VRDAYIAQNY NCVYFCMKDD YCNDLCTKNG ASSGYCQWAG KYGNACWCYA LPDNVPIRIP 


GKCHS 

« Hide

References

[1]"Purification, structure and activity of three insect toxins from Buthus occitanus tunetanus venom."
Borchani L., Stankiewicz M., Kopeyan C., Mansuelle P., Kharrat R., Cestele S., Karoui H., Rochat H., Pelhate M., el Ayeb M.
Toxicon 35:365-382(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, LETHAL DOSE, MASS SPECTROMETRY.
Tissue: Venom.

Cross-references

3D structure databases

ProteinModelPortalP55902.
SMRP55902. Positions 1-64.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSPR00285. SCORPNTOXIN.
SMARTSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMSSF57095. SSF57095. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSIX1_BUTOC
AccessionPrimary (citable) accession number: P55902
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families