Reviewed, UniProtKB/Swiss-Prot P55901 (PTEB_AERHY)

Names and origin

Protein names

Recommended name:
    Cellobiose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
Alternative name(s):
    PTS system cellobiose-specific EIIB component
    EIIB-Cel

Gene names

Name:

celA

Organism

Aeromonas hydrophila

Taxonomic identifier

644 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	88 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in cellobiose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›88	›88	Cellobiose-specific phosphotransferase enzyme IIB component		PRO_0000186488
Regions
	Domain	3 – ›88	›86	PTS EIIB type-3
Sites
	Active site	10	1	Phosphocysteine intermediate By similarity
Experimental info
	Non-terminal residue	88	1

Sequences

	Sequence		Length	Mass (Da)
	P55901-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2EA61F78BD1F30DF	FASTA	88	9,716
	10 20 30 40 50 60 MEKKRIYLFC SAGMSTSLLV SKMKAQAEKY DVPVLIDAYP ETLAGEKGQD ADLVLLGPQI 70 80 AYMLPEIQQQ LPGKPVEVID TLLYGKVD

References

[1]	"Isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in Aeromonas hydrophila." Howard S.P., Critch J., Bedi A. J. Bacteriol. 175:6695-6703(1993) [PubMed: 8407845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Ah65.
[2]	"Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes." Reizer J., Charbit A., Reizer A., Saier M.H. Jr. Genome Sci. Technol. 1:53-75(1996) Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66504 Genomic DNA. No translation available.
PIR	H49905.
3D structure databases
HSSP	HSSP built from PDB template 1H9C based on UniProtKB P17409.
SMR	P55901. Positions 3-88.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.69. 3060.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR013012. PTS_EIIB_3. IPR003501. PTS_IIB_lac. [Graphical view]
Pfam	PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTEB_AERHY

Accession

Primary (citable) accession number: P55901

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 22, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)