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Protein

Phosphoserine aminotransferase

Gene

serC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.UniRule annotation

Catalytic activityi

O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.UniRule annotation
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation

Pathwayi: L-serine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoserine aminotransferase (serC)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. D-erythrose-4-phosphate dehydrogenase (epd)
  2. Erythronate-4-phosphate dehydrogenase (pdxB)
  3. Phosphoserine aminotransferase (serC)
  4. 4-hydroxythreonine-4-phosphate dehydrogenase 1 (pdxA1), 4-hydroxythreonine-4-phosphate dehydrogenase 2 (pdxA2)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91L-glutamateUniRule annotation
Binding sitei42 – 421L-glutamateUniRule annotation
Binding sitei102 – 1021Pyridoxal phosphateUniRule annotation
Binding sitei153 – 1531Pyridoxal phosphateUniRule annotation
Binding sitei174 – 1741Pyridoxal phosphateUniRule annotation
Binding sitei197 – 1971Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Pyridoxine biosynthesis, Serine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciSENT99287:GCTI-983-MONOMER.
UniPathwayiUPA00135; UER00197.
UPA00244; UER00311.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoserine aminotransferaseUniRule annotation (EC:2.6.1.52UniRule annotation)
Alternative name(s):
Phosphohydroxythreonine aminotransferaseUniRule annotation
Short name:
PSATUniRule annotation
Gene namesi
Name:serCUniRule annotation
Ordered Locus Names:STM0977
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Phosphoserine aminotransferasePRO_0000150207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PaxDbiP55900.
PRIDEiP55900.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM0977.

Structurei

Secondary structure

1
362
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Helixi16 – 227Combined sources
Helixi51 – 6111Combined sources
Beta strandi67 – 748Combined sources
Helixi78 – 8710Combined sources
Beta strandi93 – 1008Combined sources
Helixi101 – 11010Combined sources
Turni111 – 1133Combined sources
Beta strandi114 – 1207Combined sources
Beta strandi122 – 1254Combined sources
Beta strandi128 – 1314Combined sources
Helixi134 – 1363Combined sources
Beta strandi146 – 1483Combined sources
Beta strandi150 – 1523Combined sources
Turni153 – 1564Combined sources
Beta strandi171 – 1744Combined sources
Turni176 – 1805Combined sources
Helixi186 – 1883Combined sources
Beta strandi190 – 1956Combined sources
Turni196 – 2005Combined sources
Beta strandi205 – 2117Combined sources
Helixi212 – 2143Combined sources
Helixi224 – 2263Combined sources
Helixi228 – 2336Combined sources
Helixi245 – 25814Combined sources
Helixi261 – 28121Combined sources
Beta strandi284 – 2874Combined sources
Helixi292 – 2943Combined sources
Beta strandi297 – 30610Combined sources
Helixi307 – 3093Combined sources
Helixi310 – 31910Combined sources
Turni329 – 3313Combined sources
Beta strandi333 – 3375Combined sources
Helixi344 – 36118Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QM2X-ray2.25A/B1-362[»]
ProteinModelPortaliP55900.
SMRiP55900. Positions 3-362.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55900.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 772Pyridoxal phosphate bindingUniRule annotation
Regioni239 – 2402Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QM1. Bacteria.
COG1932. LUCA.
HOGENOMiHOG000088965.
KOiK00831.
OMAiGAQKNMG.
OrthoDBiEOG60CWP3.
PhylomeDBiP55900.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00160. SerC_aminotrans_5.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR022278. Pser_aminoTfrase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000525. SerC. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01364. serC_1. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQVFNFSSG PAMLPAEVLK LAQQELCDWH GLGTSVMEIS HRGKEFIQVA
60 70 80 90 100
EEAEQDFRDL LNIPSNYKVL FCHGGGRGQF AGVPLNLLGD KTTADYVDAG
110 120 130 140 150
YWAASAIKEA KKYCAPQIID AKITVDGKRA VKPMREWQLS DNAAYLHYCP
160 170 180 190 200
NETIDGIAID ETPDFGPEVV VTADFSSTIL SAPLDVSRYG VIYAGAQKNI
210 220 230 240 250
GPAGLTLVIV REDLLGKAHE SCPSILDYTV LNDNDSMFNT PPTFAWYLSG
260 270 280 290 300
LVFKWLKAQG GVAAMHKINQ QKAELLYGVI DNSDFYRNDV AQANRSRMNV
310 320 330 340 350
PFQLADNTLD KVFLEESFAA GLHALKGHRV VGGMRASIYN AMPIEGVKAL
360
TDFMIDFERR HG
Length:362
Mass (Da):39,832
Last modified:January 23, 2002 - v2
Checksum:i802C026A8B4CE2C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471I → M in CAA71381 (Ref. 1) Curated
Sequence conflicti79 – 791Q → T in CAA71381 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10355 Genomic DNA. Translation: CAA71381.1.
AE006468 Genomic DNA. Translation: AAL19911.1.
RefSeqiNP_459952.1. NC_003197.1.
WP_000079584.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19911; AAL19911; STM0977.
GeneIDi1252495.
KEGGistm:STM0977.
PATRICi32380367. VBISalEnt20916_1030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10355 Genomic DNA. Translation: CAA71381.1.
AE006468 Genomic DNA. Translation: AAL19911.1.
RefSeqiNP_459952.1. NC_003197.1.
WP_000079584.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QM2X-ray2.25A/B1-362[»]
ProteinModelPortaliP55900.
SMRiP55900. Positions 3-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0977.

Proteomic databases

PaxDbiP55900.
PRIDEiP55900.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19911; AAL19911; STM0977.
GeneIDi1252495.
KEGGistm:STM0977.
PATRICi32380367. VBISalEnt20916_1030.

Phylogenomic databases

eggNOGiENOG4107QM1. Bacteria.
COG1932. LUCA.
HOGENOMiHOG000088965.
KOiK00831.
OMAiGAQKNMG.
OrthoDBiEOG60CWP3.
PhylomeDBiP55900.

Enzyme and pathway databases

UniPathwayiUPA00135; UER00197.
UPA00244; UER00311.
BioCyciSENT99287:GCTI-983-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP55900.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00160. SerC_aminotrans_5.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR022278. Pser_aminoTfrase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000525. SerC. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01364. serC_1. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mouslim C., Flores A., Cano D.A., Casadesus J.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiSERC_SALTY
AccessioniPrimary (citable) accession number: P55900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: February 17, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.