P55885 (GAST_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrin

Cleaved into the following 2 chains:

1. Big gastrin
   Alternative name(s):
   Gastrin-34
   Short name=G34
2. Gastrin

Gene names

Name:	GAST
Synonyms:	GAS

Organism

Equus caballus (Horse) [Complete proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Subcellular location	Secreted.
Post-translational modification	Neither gastrin is sulfated.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.
Mass spectrometry	Molecular mass is 1928 Da from positions 63 - 78. Determined by MALDI. Ref.1 Molecular mass is 3814 Da from positions 62 - 95. Determined by MALDI. Ref.1 Molecular mass is 3703 Da from positions 63 - 95. Determined by MALDI. Ref.1 Molecular mass is 2039 Da from positions 80 - 95. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Phosphoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Propeptide	22 – 61	40			PRO_0000010629
	Peptide	62 – 95	34	Big gastrin Ref.1		PRO_0000010630
	Peptide	79 – 95	17	Gastrin		PRO_0000010631
	Propeptide	99 – 107	9			PRO_0000010632
Amino acid modifications
	Modified residue	62	1	Pyrrolidone carboxylic acid
	Modified residue	79	1	Pyrrolidone carboxylic acid
	Modified residue	95	1	Phenylalanine amide Ref.1
	Modified residue	99	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55885 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 104166CAAE5C234F	FASTA	107	11,884
	10 20 30 40 50 60 MRRLCVYVLI FALALAAFSE ASWKPCSQLQ DAPSGPGANK GLYPHWPDQL DRLGPASHHR 70 80 90 100 RQLGLQGSPH LVADLSKKQG PWLEKEEAAY GWMDFGRRSA EEGDQSP

References

[1]

"Unique progastrin processing in equine G-cells suggests marginal tyrosyl sulfotransferase activity." Johnsen A.H., Sandin A., Rourke I.J., Bundgaard J.R., Nilsson G., Rehfeld J.F. Eur. J. Biochem. 255:432-438(1998) [PubMed: 9716385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 62-95, AMIDATION AT PHE-95, PYROGLUTAMATE FORMATION AT GLN-62 AND GLN-79, MASS SPECTROMETRY. Tissue: Gastric mucosa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09440 mRNA. Translation: CAA70590.1.
RefSeq	NP_001075979.1. NM_001082510.1.
UniGene	Eca.13044.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P55885.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100034214.
KEGG	ecb:100034214.
Organism-specific databases
CTD	2520.
Phylogenomic databases
GeneTree	ENSGT00390000014792.
HOVERGEN	HBG097593.
InParanoid	P55885.
OrthoDB	EOG4CG09S.
Family and domain databases
InterPro	IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
KO	K13768.
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAST_HORSE

Accession

Primary (citable) accession number: P55885

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program