Reviewed, UniProtKB/Swiss-Prot P55866 (CASP3_XENLA)

Names and origin

Protein names

Recommended name:
    Caspase-3
      Short name=CASP-3
    EC=3.4.22.56
Alternative name(s):
    Apopain
    Cysteine protease CPP32
      Short name=xCPP32
Cleaved into the following 2 chains:
    1- Recommended name:
            Caspase-3 subunit p17
    2- Recommended name:
            Caspase-3 subunit p12

Gene names

Name:

casp3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Important mediator of apoptosis. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond By similarity.
Catalytic activity	Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The subunits are derived from the precursor sequence by a probable autocatalytic mechanism and probably by other caspases By similarity.
Sequence similarities	Belongs to the peptidase C14A family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – ?		By similarity		PRO_0000004593
	Chain	? – 186		Caspase-3 subunit p17		PRO_0000004594
	Chain	187 – 282	96	Caspase-3 subunit p12		PRO_0000004595
Sites
	Active site	131	1	By similarity
	Active site	174	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55866-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: CB390E6980CAB77F	FASTA	282	32,125
	10 20 30 40 50 60 MEESQNGVKY GGDATDAKEY FTIQPRSLQN CDLKDIERKT KFAHLQNYRT NYPEMGMCLI 70 80 90 100 110 120 INNKNFHSSN MAVRNGTDVD ALKLHETFTG LGYEVMVCND QKSSDIIGRL KKISEEDHSK 130 140 150 160 170 180 RSSFVCAILS HGEEDGSICG VDVPIHIKNL TDLFRGDRCK TLVGKPKIFF IQACRGTELD 190 200 210 220 230 240 SGIETDSCSE PREEIQRIPV EADFLYAYST VPGYCSWRDK MDGSWFIQSL CKMIKLYGSH 250 260 270 280 LELIQILTCV NHMVALDFET FHAKKQIPCV VSMLTKSFYF FK

References

[1]	"Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail." Yaoita Y., Nakajima K. J. Biol. Chem. 272:5122-5127(1997) [PubMed: 9030578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D89784 mRNA. Translation: BAA14018.1.
RefSeq	NP_001081225.1.
UniGene	Xl.17957
3D structure databases
HSSP	HSSP built from PDB template 1PAU based on UniProtKB P42574.
ModBase	Search...
Genome annotation databases
GeneID	397720.
KEGG	xla:397720.
Organism-specific databases
CTD	397720.
Xenbase	XB-FEAT-479959. casp3.
Phylogenomic databases
HOVERGEN	P55866.
Enzyme and pathway databases
BRENDA	3.4.22.56. 648.
Family and domain databases
InterPro	IPR015470. Caspase_3_related. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454:SF30. Casp3_like. 1 hit. PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP3_XENLA

Accession

Primary (citable) accession number: P55866

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 1, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project