Reviewed, UniProtKB/Swiss-Prot P55859 (PNPH_BOVIN)

Names and origin

Protein names

Recommended name:
    Purine nucleoside phosphorylase
      Short name=PNP
    EC=2.4.2.1
Alternative name(s):
    Inosine phosphorylase

Gene names

Name:	NP
Synonyms:	PNP

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	purine-nucleoside phosphorylase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 289

289

Purine nucleoside phosphorylase

PRO_0000184535

Amino acid modifications

Modified residue

1

1

N-acetylmethionine By similarity

Experimental info

Sequence conflict

2

1

A → Q AA sequence Ref.1

Sequence conflict

25

1

P → S in AAX46392. Ref.2

Secondary structure

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289

Helix Strand Turn

Details...

Helix

7 – 18

12

Beta strand

26 – 31

6

Helix

33 – 41

9

Beta strand

43 – 49

7

Turn

50 – 52

3

Beta strand

62 – 64

3

Beta strand

67 – 73

7

Beta strand

76 – 83

8

Helix

87 – 89

3

Helix

93 – 96

4

Helix

98 – 105

8

Beta strand

110 – 119

10

Beta strand

129 – 137

9

Helix

138 – 141

4

Turn

153 – 155

3

Helix

168 – 180

13

Beta strand

188 – 194

7

Helix

203 – 211

9

Beta strand

215 – 221

7

Helix

222 – 230

9

Beta strand

234 – 244

11

Beta strand

248 – 250

3

Helix

257 – 260

4

Turn

261 – 265

5

Helix

266 – 278

13

Turn

279 – 281

3

Beta strand

284 – 287

4

Sequences

	Sequence		Length	Mass (Da)
	P55859-1 [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 7ECF84CCA494DEED	FASTA	289	32,037
	10 20 30 40 50 60 MANGYTYEDY QDTAKWLLSH TEQRPQVAVI CGSGLGGLVN KLTQAQTFDY SEIPNFPEST 70 80 90 100 110 120 VPGHAGRLVF GILNGRACVM MQGRFHMYEG YPFWKVTFPV RVFRLLGVET LVVTNAAGGL 130 140 150 160 170 180 NPNFEVGDIM LIRDHINLPG FSGENPLRGP NEERFGVRFP AMSDAYDRDM RQKAHSTWKQ 190 200 210 220 230 240 MGEQRELQEG TYVMLGGPNF ETVAECRLLR NLGADAVGMS TVPEVIVARH CGLRVFGFSL 250 260 270 280 ITNKVIMDYE SQGKANHEEV LEAGKQAAQK LEQFVSLLMA SIPVSGHTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Calf spleen purine nucleoside phosphorylase: purification, sequence and crystal structure of its complex with an N(7)-acycloguanosine inhibitor." Bzowska A., Luic M., Schroeder W., Shugar D., Saenger W., Koellner G. FEBS Lett. 367:214-218(1995) [PubMed: 7607309] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Spleen.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.
[4]	"Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15-A resolution." Koellner G., Luic M., Shugar D., Saenger W., Bzowska A. J. Mol. Biol. 265:202-216(1997) [PubMed: 9020983] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). Tissue: Spleen.
[5]	Mao C., Zhou M., Ealick S., Federov A.A., Almo S.C. Submitted (JUL-1995) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
[6]	"Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues." Mao C., Cook W.J., Zhou M., Federov A.A., Almo S.C., Ealick S.E. Biochemistry 37:7135-7146(1998) [PubMed: 9585525] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). Tissue: Spleen.
[7]	"The high resolution crystal structure of bovine spleen purine nucleoside phosphorylase in complex forms with phosphate and 9-deazainosine." Pugmire M.J., Mao C., Ealick S.E. Submitted (MAR-1998) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BT021545 mRNA. Translation: AAX46392.1.
BC103291 mRNA. Translation: AAI03292.2.

IPI

IPI00717573.

PIR

S66203.

UniGene

Bt.65253

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A9O	X-ray	2.00	A	3-289	[»]
1A9P	X-ray	2.40	A	3-289	[»]
1A9Q	X-ray	2.00	A	3-282	[»]
1A9R	X-ray	2.00	A	3-282	[»]
1A9S	X-ray	2.00	A	3-289	[»]
1A9T	X-ray	2.00	A	1-284	[»]
1B8N	X-ray	2.00	A	3-284	[»]
1B8O	X-ray	1.50	A	3-284	[»]
1FXU	X-ray	2.20	A	3-289	[»]
1LV8	X-ray	2.30	A/B/C/D/E/F	3-289	[»]
1LVU	X-ray	2.05	A/B/C/D/E/F	3-289	[»]
1PBN	X-ray	2.00	A	1-289	[»]
1V48	X-ray	2.20	A	3-289	[»]
1VFN	X-ray	2.15	A	4-284	[»]
2AI1	X-ray	2.00	A	3-289	[»]
2AI2	X-ray	1.70	A	3-289	[»]
2AI3	X-ray	1.70	A	3-289	[»]
2QPL	X-ray	2.10	A	3-284	[»]
3PNP	X-ray	1.60	A	1-289	[»]
4PNP	X-ray	1.80	A	1-289	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000012317. Bos taurus. [Contig view]

Phylogenomic databases

HOVERGEN

P55859.

Enzyme and pathway databases

BRENDA

2.4.2.1. 251.

Family and domain databases

InterPro

IPR000845. Nucleoside_phosphorylase.
IPR011268. PNPHPUNA_XAPA.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR001369. Purine_phosphorylase-2.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]

PANTHER

PTHR11904. Mtap_PNP. 1 hit.
PTHR11904:SF9. PNPHPUNA_XAPA. 1 hit.

Pfam

PF01048. PNP_UDP_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.

PROSITE

PS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P55859.

Entry information

Entry name

PNPH_BOVIN

Accession

Primary (citable) accession number: P55859
Secondary accession number(s): Q3ZBH6, Q58DQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)