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Protein

Small ubiquitin-related modifier

Gene

smo-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process (PubMed:11806825). Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction (PubMed:11806825, PubMed:25475837). Covalent attachment to its substrates requires prior activation by the E1 complex aos-1-uba-2 and linkage to the E2 enzyme ubc-9, and can be promoted by an E3 ligase such as gei-17 (PubMed:15107848, PubMed:16701625). Required for embryonic development, fertility, vulval morphogenesis and inhibition of vulval cell fates (PubMed:15466489, PubMed:15689373, PubMed:15990876, PubMed:24349540). Probably by sumoylating bet-1, prevents muscle myosin depletion in aging adults probably by preventing myoblast growth factor receptor egl-15 overexpression (PubMed:24285704). Plays a role in the attenuation of the let-60/ras pathway (PubMed:24349540, PubMed:24285704).8 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein tag Source: GO_Central

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • mitotic metaphase plate congression Source: WormBase
  • mitotic sister chromatid separation Source: WormBase
  • negative regulation of transcription from RNA polymerase II promoter Source: WormBase
  • nematode larval development Source: WormBase
  • protein localization to chromosome Source: WormBase
  • protein sumoylation Source: WormBase
  • synaptonemal complex disassembly Source: WormBase

Keywordsi

Molecular functionDevelopmental protein
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-CEL-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).
R-CEL-3065679. SUMO is proteolytically processed.
R-CEL-3108214. SUMOylation of DNA damage response and repair proteins.
R-CEL-3232118. SUMOylation of transcription factors.
R-CEL-4551638. SUMOylation of chromatin organization proteins.
R-CEL-4570464. SUMOylation of RNA binding proteins.
R-CEL-4615885. SUMOylation of DNA replication proteins.
R-CEL-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-CEL-5696395. Formation of Incision Complex in GG-NER.
R-CEL-877312. Regulation of IFNG signaling.
R-CEL-8866904. Negative regulation of activity of TFAP2 (AP-2) family transcription factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Small ubiquitin-related modifier
Short name:
SUMO
Alternative name(s):
Ubiquitin-like protein SMT3
Gene namesi
Name:smo-1
Synonyms:smt3, sumo
ORF Names:K12C11.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiK12C11.2; CE18056; WBGene00004888; smo-1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes chromosome misalignment and anaphase bridges during the first embryonic mitotic division (PubMed:25475837). 23 percent of mutant animals have impaired locomotion (PubMed:24285704). Ectopic expression of egl-17 in multiple vulva precursor cells and moderate increase in phosphorylation of mpk-1 (PubMed:24349540). Adult bet-1 and smo-1 double mutants have decreased myo-3 levels in muscles and increased transcription levels of egl-15, sur-1 and let-60 (PubMed:24285704).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001148901 – 90Small ubiquitin-related modifierAdd BLAST90
PropeptideiPRO_000027122791By similarity1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki90Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Post-translational modificationi

Cleavage of precursor form by ulp-1 is necessary for function.Curated

Keywords - PTMi

Isopeptide bond

Proteomic databases

EPDiP55853.
PaxDbiP55853.
PeptideAtlasiP55853.
PRIDEiP55853.

PTM databases

iPTMnetiP55853.

Expressioni

Gene expression databases

BgeeiWBGene00004888.

Interactioni

Subunit structurei

Covalently attached to tbx-2 (PubMed:16701625). Covalently attached to lin-1 (PubMed:15689373). Covalently attached to lin-11 (PubMed:15466489). Covalently attached to sop-2 (PubMed:15107848). Covalently attached to bet-1 (PubMed:24349540, PubMed:24285704).6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi57309. 30 interactors.
DIPiDIP-25461N.
IntActiP55853. 36 interactors.
MINTiMINT-1071902.
STRINGi6239.K12C11.2.

Structurei

3D structure databases

ProteinModelPortaliP55853.
SMRiP55853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 91Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ubiquitin family. SUMO subfamily.Curated

Phylogenomic databases

eggNOGiKOG1769. Eukaryota.
COG5227. LUCA.
GeneTreeiENSGT00390000018808.
HOGENOMiHOG000207495.
InParanoidiP55853.
KOiK12160.
OMAiYIKIKVV.
OrthoDBiEOG091G10ZQ.
PhylomeDBiP55853.

Family and domain databases

CDDicd01763. Sumo. 1 hit.
InterProiView protein in InterPro
IPR022617. Rad60/SUMO-like_dom.
IPR033950. Sumo.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF11976. Rad60-SLD. 1 hit.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55853-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDAAQAGD NAEYIKIKVV GQDSNEVHFR VKYGTSMAKL KKSYADRTGV
60 70 80 90
AVNSLRFLFD GRRINDDDTP KTLEMEDDDV IEVYQEQLGG F
Length:91
Mass (Da):10,222
Last modified:November 1, 1997 - v1
Checksum:i0894E99B6F7B37F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99600 mRNA. Translation: CAA67914.1.
U94830 Genomic DNA. Translation: AAB67608.1.
FO081631 Genomic DNA. Translation: CCD72942.1.
PIRiJC5582.
RefSeqiNP_490842.1. NM_058441.4.
UniGeneiCel.18381.

Genome annotation databases

EnsemblMetazoaiK12C11.2; K12C11.2; WBGene00004888.
GeneIDi266820.
KEGGicel:CELE_K12C11.2.
UCSCiK12C11.2.1. c. elegans.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSUMO_CAEEL
AccessioniPrimary (citable) accession number: P55853
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 7, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families