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Protein

Desmocollin-3

Gene

Dsc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.

GO - Molecular functioni

GO - Biological processi

  • homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
  • in utero embryonic development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Desmocollin-3
Gene namesi
Name:Dsc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1194993. Dsc3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini136 – 695ExtracellularSequence analysisAdd BLAST560
Transmembranei696 – 716HelicalSequence analysisAdd BLAST21
Topological domaini717 – 896CytoplasmicSequence analysisAdd BLAST180

GO - Cellular componenti

  • cytoplasm Source: MGI
  • desmosome Source: MGI
  • extracellular region Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
PropeptideiPRO_000000387732 – 135Sequence analysisAdd BLAST104
ChainiPRO_0000003878136 – 896Desmocollin-3Add BLAST761

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Modified residuei386PhosphothreonineBy similarity1
Glycosylationi392N-linked (GlcNAc...)Sequence analysis1
Glycosylationi547N-linked (GlcNAc...)Sequence analysis1
Glycosylationi630N-linked (GlcNAc...) (high mannose)1 Publication1
Modified residuei859PhosphoserineBy similarity1
Modified residuei863PhosphoserineBy similarity1
Modified residuei868PhosphoserineBy similarity1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP55850.
PeptideAtlasiP55850.
PRIDEiP55850.

PTM databases

PhosphoSitePlusiP55850.
UniCarbKBiP55850.

Expressioni

Tissue specificityi

First expressed at E13.0 in epithelium of whisker pads and external nares, and in most mature vibrissa follicles. 12 hours later, prominently expressed in whiskers and tactile follicles above the eye. At E14.5, also expressed in developing nails and teeth and, at low levels, in ventral and lateral skin. At E15.5, highly expressed in general body epidermis and at E16.5, detected over entire embryo. In the adult, highly expressed in basal layers of stratified cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000059898.
CleanExiMM_DSC3.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996857.
STRINGi10090.ENSMUSP00000111514.

Structurei

3D structure databases

ProteinModelPortaliP55850.
SMRiP55850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 243Cadherin 1PROSITE-ProRule annotationAdd BLAST108
Domaini244 – 355Cadherin 2PROSITE-ProRule annotationAdd BLAST112
Domaini356 – 472Cadherin 3PROSITE-ProRule annotationAdd BLAST117
Domaini473 – 580Cadherin 4PROSITE-ProRule annotationAdd BLAST108
Domaini581 – 691Cadherin 5PROSITE-ProRule annotationAdd BLAST111

Domaini

Calcium may be bound by the cadherin-like repeats.Curated
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.By similarity

Sequence similaritiesi

Contains 5 cadherin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
GeneTreeiENSGT00760000118906.
HOGENOMiHOG000231253.
HOVERGENiHBG102801.
InParanoidiP55850.
KOiK07602.
OMAiFCGTMGS.
OrthoDBiEOG091G01IB.

Family and domain databases

Gene3Di2.60.40.60. 6 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR014868. Cadherin_pro_dom.
IPR027397. Catenin_binding_dom.
IPR009122. Desmosomal_cadherin.
[Graphical view]
PANTHERiPTHR24025. PTHR24025. 2 hits.
PfamiPF00028. Cadherin. 4 hits.
PF01049. Cadherin_C. 1 hit.
PF08758. Cadherin_pro. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
PR01818. DESMOCADHERN.
SMARTiSM00112. CA. 5 hits.
SM01055. Cadherin_pro. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 6 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3A (identifier: P55850-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVPEFRSPQ CRALCTKLLL TLWVFSFVGE ACKKVTFHVP STLEADKIIG
60 70 80 90 100
RVSLKECLSS ADGIMPSDPD FRVLDDGSVY PTRAVVLSDE KRSFTIQLSD
110 120 130 140 150
SKMQTQKEIP VILEHKKKVL KKRHTKETVL RRSKRRWAPI PCSMQENSLG
160 170 180 190 200
PFPLFLQQVQ SDAAQNYTVF YSISGRGADQ EPLNWFFIER DTGNLYCTRP
210 220 230 240 250
VDREEYDVFD LIAYASTADG YSADLPLPLP IKIEDENDNY PLFTEAIYAF
260 270 280 290 300
EVPEGSRLGT VVGTVCATDK DEPDTMHTRL KYSILEQTPP SPGLFSVHPD
310 320 330 340 350
TGVITTVSHY MDREVVDKYK LIMKVQDMNG QFFGLISTST CIITVQDSND
360 370 380 390 400
NAPTFRQNTY ETAVEENTYN VEILRIPVDD KDMINTANWK ANFTILKGNE
410 420 430 440 450
NGWFKITTDP VTNEGVLCVV KPLDYEENRQ VTLEIGVNNE APFIKDVANR
460 470 480 490 500
IPTMNRAMVT VHVKDQNEGP ECKPPEQYVR IKENSAVGSK INGYKAYDPE
510 520 530 540 550
TKNSNGLRYK KLQDPKDWVS IEEVSGLLTI SKTLDREIMA PRNDMYNITV
560 570 580 590 600
MAIDQEGKSC TGTLAVNIED VNDNAPEIIQ DYIVICKPKM GYTDISAVDP
610 620 630 640 650
DEPIHGPPFQ FNLANTSPEV NRIWTLNQVN DTAARLSYQK TADVQIYNVP
660 670 680 690 700
VTVKDRAGQS ATKILRVNLC DCTHPSQCPL RSRSAGITLG KWAILAILLG
710 720 730 740 750
IALLFSVLLT LVCGVVTARK GKHFPEDLAQ QNLIISNTEA PGDDRVCSAN
760 770 780 790 800
GFTTHTANNS SQGFCGTMGS GMRNGGQETI EMMKGHQTLD SCRVAGHHHT
810 820 830 840 850
LDSGRGGHMD TDNCRYTYSE WHSFTQPRLG EKLHVCNQNE DHIPSQDYVL
860 870 880 890
TYNYEGRGSP AGSVGCCSEK QEEEGLDFLN NLEPKFLTLA ETCTKR
Length:896
Mass (Da):100,203
Last modified:March 16, 2016 - v3
Checksum:iDC630F26CD00CB3D
GO
Isoform 3B (identifier: P55850-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     832-839: KLHVCNQN → DSIRGHTG
     840-896: Missing.

Show »
Length:839
Mass (Da):93,724
Checksum:i054B1A1BD408F194
GO

Sequence cautioni

The sequence CAA72045 differs from that shown. Reason: Frameshift at position 768.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45A → V in CAA72045 (PubMed:9389456).Curated1
Sequence conflicti435I → T in CAA72045 (PubMed:9389456).Curated1
Sequence conflicti539M → I in CAA72045 (PubMed:9389456).Curated1
Sequence conflicti626L → I in CAA72045 (PubMed:9389456).Curated1
Sequence conflicti694I → Y in CAA72045 (PubMed:9389456).Curated1
Sequence conflicti715V → I in CAA03996 (PubMed:9404003).Curated1
Sequence conflicti730 – 731QQ → HE in CAA72045 (PubMed:9389456).Curated2
Sequence conflicti730 – 731QQ → HE in CAA03996 (PubMed:9404003).Curated2
Sequence conflicti841D → N in CAA72045 (PubMed:9389456).Curated1
Sequence conflicti891E → K in CAA72045 (PubMed:9389456).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000665832 – 839KLHVCNQN → DSIRGHTG in isoform 3B. Curated8
Alternative sequenceiVSP_000666840 – 896Missing in isoform 3B. CuratedAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11169 mRNA. Translation: CAA72045.1. Frameshift.
AC105159 Genomic DNA. No translation available.
CH466557 Genomic DNA. Translation: EDK96937.1.
AJ000329 mRNA. Translation: CAA03996.1.
CCDSiCCDS37744.1. [P55850-1]
RefSeqiNP_001278738.1. NM_001291809.1. [P55850-2]
NP_031908.3. NM_007882.3. [P55850-1]
XP_006525656.1. XM_006525593.3. [P55850-1]
UniGeneiMm.89935.

Genome annotation databases

EnsembliENSMUST00000115848; ENSMUSP00000111514; ENSMUSG00000059898. [P55850-1]
GeneIDi13507.
KEGGimmu:13507.
UCSCiuc008eea.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11169 mRNA. Translation: CAA72045.1. Frameshift.
AC105159 Genomic DNA. No translation available.
CH466557 Genomic DNA. Translation: EDK96937.1.
AJ000329 mRNA. Translation: CAA03996.1.
CCDSiCCDS37744.1. [P55850-1]
RefSeqiNP_001278738.1. NM_001291809.1. [P55850-2]
NP_031908.3. NM_007882.3. [P55850-1]
XP_006525656.1. XM_006525593.3. [P55850-1]
UniGeneiMm.89935.

3D structure databases

ProteinModelPortaliP55850.
SMRiP55850.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996857.
STRINGi10090.ENSMUSP00000111514.

PTM databases

PhosphoSitePlusiP55850.
UniCarbKBiP55850.

Proteomic databases

PaxDbiP55850.
PeptideAtlasiP55850.
PRIDEiP55850.

Protocols and materials databases

DNASUi13507.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115848; ENSMUSP00000111514; ENSMUSG00000059898. [P55850-1]
GeneIDi13507.
KEGGimmu:13507.
UCSCiuc008eea.2. mouse.

Organism-specific databases

CTDi1825.
MGIiMGI:1194993. Dsc3.

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
GeneTreeiENSGT00760000118906.
HOGENOMiHOG000231253.
HOVERGENiHBG102801.
InParanoidiP55850.
KOiK07602.
OMAiFCGTMGS.
OrthoDBiEOG091G01IB.

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Miscellaneous databases

PROiP55850.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059898.
CleanExiMM_DSC3.

Family and domain databases

Gene3Di2.60.40.60. 6 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR014868. Cadherin_pro_dom.
IPR027397. Catenin_binding_dom.
IPR009122. Desmosomal_cadherin.
[Graphical view]
PANTHERiPTHR24025. PTHR24025. 2 hits.
PfamiPF00028. Cadherin. 4 hits.
PF01049. Cadherin_C. 1 hit.
PF08758. Cadherin_pro. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
PR01818. DESMOCADHERN.
SMARTiSM00112. CA. 5 hits.
SM01055. Cadherin_pro. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 6 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDSC3_MOUSE
AccessioniPrimary (citable) accession number: P55850
Secondary accession number(s): G5E8S6, O55110, O55122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 16, 2016
Last modified: November 30, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.