Reviewed, UniProtKB/Swiss-Prot P55850 (DSC3_MOUSE)

Names and origin

Protein names

Recommended name:
    Desmocollin-3

Gene names

Name:

Dsc3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	895 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
Subcellular location	Cell membrane; Single-pass type I membrane protein Potential. Cell junction › desmosome By similarity.
Tissue specificity	First expressed at E13.0 in epithelium of whisker pads and external nares, and in most mature vibrissa follicles. 12 hours later, prominently expressed in whiskers and tactile follicles above the eye. At E14.5, also expressed in developing nails and teeth and, at low levels, in ventral and lateral skin. At E15.5, highly expressed in general body epidermis and at E16.5, detected over entire embryo. In the adult, highly expressed in basal layers of stratified cells. Ref.1 Ref.2
Domain	Calcium may be bound by the cadherin-like repeats Potential.
Sequence similarities	Contains 5 cadherin domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane
Ligand	Calcium
PTM	Cleavage on pair of basic residues Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	homophilic cell adhesion Inferred from electronic annotation. Source: InterPro in utero embryonic development Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI desmosome Inferred from direct assay. Source: MGI extracellular region Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3A (identifier: P55850-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3B (identifier: P55850-2)

The sequence of this isoform differs from the canonical sequence as follows:
     831-838: KLHVCNQN → DSIRGHTG
     839-895: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Propeptide	32 – 135	104	Potential		PRO_0000003877
	Chain	136 – 895	760	Desmocollin-3		PRO_0000003878
Regions
	Topological domain	136 – 695	560	Extracellular Potential
	Transmembrane	696 – 716	21	Potential
	Topological domain	717 – 895	179	Cytoplasmic Potential
	Domain	136 – 243	108	Cadherin 1
	Domain	244 – 355	112	Cadherin 2
	Domain	356 – 472	117	Cadherin 3
	Domain	473 – 580	108	Cadherin 4
	Domain	581 – 691	111	Cadherin 5
Amino acid modifications
	Modified residue	817	1	Phosphotyrosine By similarity
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Glycosylation	547	1	N-linked (GlcNAc...) Potential
	Glycosylation	630	1	N-linked (GlcNAc...) (high mannose) Ref.3
Natural variations
	Alternative sequence	831 – 838	8	KLHVCNQN → DSIRGHTG in isoform 3B.		VSP_000665
	Alternative sequence	839 – 895	57	Missing in isoform 3B.		VSP_000666
Experimental info
	Sequence conflict	715	1	V → I in CAA03996. Ref.2
	Sequence conflict	750 – 767	18	WIHHP…LLWYN → NGFTTHTANNSSQGFCGTM in CAA03996. Ref.2
	Sequence conflict	840	1	N → D in CAA03996. Ref.2
	Sequence conflict	873 – 874	2	EG → IK Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3A [UniParc]. Last modified June 1, 2001. Version 2. Checksum: D561E67C57232270	FASTA	895	100,654
	10 20 30 40 50 60 MVVPEFRSPQ CRALCTKLLL TLWVFSFVGE ACKKVTFHVP STLEVDKIIG RVSLKECLSS 70 80 90 100 110 120 ADGIMPSDPD FRVLDDGSVY PTRAVVLSDE KRSFTIQLSD SKMQTQKEIP VILEHKKKVL 130 140 150 160 170 180 KKRHTKETVL RRSKRRWAPI PCSMQENSLG PFPLFLQQVQ SDAAQNYTVF YSISGRGADQ 190 200 210 220 230 240 EPLNWFFIER DTGNLYCTRP VDREEYDVFD LIAYASTADG YSADLPLPLP IKIEDENDNY 250 260 270 280 290 300 PLFTEAIYAF EVPEGSRLGT VVGTVCATDK DEPDTMHTRL KYSILEQTPP SPGLFSVHPD 310 320 330 340 350 360 TGVITTVSHY MDREVVDKYK LIMKVQDMNG QFFGLISTST CIITVQDSND NAPTFRQNTY 370 380 390 400 410 420 ETAVEENTYN VEILRIPVDD KDMINTANWK ANFTILKGNE NGWFKITTDP VTNEGVLCVV 430 440 450 460 470 480 KPLDYEENRQ VTLETGVNNE APFIKDVANR IPTMNRAMVT VHVKDQNEGP ECKPPEQYVR 490 500 510 520 530 540 IKENSAVGSK INGYKAYDPE TKNSNGLRYK KLQDPKDWVS IEEVSGLLTI SKTLDREIIA 550 560 570 580 590 600 PRNDMYNITV MAIDQEGKSC TGTLAVNIED VNDNAPEIIQ DYIVICKPKM GYTDISAVDP 610 620 630 640 650 660 DEPIHGPPFQ FNLANTSPEV NRIWTINQVN DTAARLSYQK TADVQIYNVP VTVKDRAGQS 670 680 690 700 710 720 ATKILRVNLC DCTHPSQCPL RSRSAGITLG KWAYLAILLG IALLFSVLLT LVCGVVTARK 730 740 750 760 770 780 GKHFPEDLAH ENLIISNTEA PGDDRVCSAW IHHPHCQQLQ PRLLWYNGSG MRNGGQETIE 790 800 810 820 830 840 MMKGHQTLDS CRVAGHHHTL DSGRGGHMDT DNCRYTYSEW HSFTQPRLGE KLHVCNQNEN 850 860 870 880 890 HIPSQDYVLT YNYEGRGSPA GSVGCCSEKQ EEEGLDFLNN LEPKFLTLAK TCTKR
	Isoform 3B. Checksum: FBAF0BD509C8B237	FASTA	838	94,177
	10 20 30 40 50 60 MVVPEFRSPQ CRALCTKLLL TLWVFSFVGE ACKKVTFHVP STLEVDKIIG RVSLKECLSS 70 80 90 100 110 120 ADGIMPSDPD FRVLDDGSVY PTRAVVLSDE KRSFTIQLSD SKMQTQKEIP VILEHKKKVL 130 140 150 160 170 180 KKRHTKETVL RRSKRRWAPI PCSMQENSLG PFPLFLQQVQ SDAAQNYTVF YSISGRGADQ 190 200 210 220 230 240 EPLNWFFIER DTGNLYCTRP VDREEYDVFD LIAYASTADG YSADLPLPLP IKIEDENDNY 250 260 270 280 290 300 PLFTEAIYAF EVPEGSRLGT VVGTVCATDK DEPDTMHTRL KYSILEQTPP SPGLFSVHPD 310 320 330 340 350 360 TGVITTVSHY MDREVVDKYK LIMKVQDMNG QFFGLISTST CIITVQDSND NAPTFRQNTY 370 380 390 400 410 420 ETAVEENTYN VEILRIPVDD KDMINTANWK ANFTILKGNE NGWFKITTDP VTNEGVLCVV 430 440 450 460 470 480 KPLDYEENRQ VTLETGVNNE APFIKDVANR IPTMNRAMVT VHVKDQNEGP ECKPPEQYVR 490 500 510 520 530 540 IKENSAVGSK INGYKAYDPE TKNSNGLRYK KLQDPKDWVS IEEVSGLLTI SKTLDREIIA 550 560 570 580 590 600 PRNDMYNITV MAIDQEGKSC TGTLAVNIED VNDNAPEIIQ DYIVICKPKM GYTDISAVDP 610 620 630 640 650 660 DEPIHGPPFQ FNLANTSPEV NRIWTINQVN DTAARLSYQK TADVQIYNVP VTVKDRAGQS 670 680 690 700 710 720 ATKILRVNLC DCTHPSQCPL RSRSAGITLG KWAYLAILLG IALLFSVLLT LVCGVVTARK 730 740 750 760 770 780 GKHFPEDLAH ENLIISNTEA PGDDRVCSAW IHHPHCQQLQ PRLLWYNGSG MRNGGQETIE 790 800 810 820 830 MMKGHQTLDS CRVAGHHHTL DSGRGGHMDT DNCRYTYSEW HSFTQPRLGE DSIRGHTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Changing pattern of desmocollin 3 expression accompanies epidermal organisation during skin development." Chidgey M.A.J., Yue K.K.M., Gould S., Byrne C., Garrod D.R. Dev. Dyn. 210:315-327(1997) [PubMed: 9389456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Hierarchical expression of desmosomal cadherins during stratified epithelial morphogenesis in the mouse." King I.A., Angst B.D., Hunt D.M., Kruger M., Arnemann J., Buxton R.S. Differentiation 62:83-96(1997) [PubMed: 9404003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 709-874, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[3]	"High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis." Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S. Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed: 16170054] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-630, MASS SPECTROMETRY. Tissue: Epidermis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11169 mRNA. Translation: CAA72045.1. AJ000329 mRNA. Translation: CAA03996.1.
IPI	IPI00113853. IPI00876127.
RefSeq	NP_031908.3.
UniGene	Mm.89935
3D structure databases
HSSP	HSSP built from PDB template 1NCJ based on UniProtKB P15116.
ModBase	Search...
PTM databases
PhosphoSite	P55850.
Genome annotation databases
Ensembl	ENSMUSG00000059898. Mus musculus. [Contig view]
GeneID	13507.
KEGG	mmu:13507.
Organism-specific databases
MGI	MGI:1194993. Dsc3.
Phylogenomic databases
HOGENOM	P55850.
HOVERGEN	P55850.
Gene expression databases
ArrayExpress	P55850.
Bgee	P55850.
CleanEx	MM_DSC3.
GermOnline	ENSMUSG00000059898. Mus musculus.
Family and domain databases
InterPro	IPR002126. Cadherin. IPR000233. Cadherin_C_term. IPR014868. Cadherin_pro. IPR009122. Desmo_cadherin. IPR009124. Desmocollin. [Graphical view]
Gene3D	G3DSA:2.60.40.60. Cadherin. 4 hits.
Pfam	PF00028. Cadherin. 5 hits. PF01049. Cadherin_C. 1 hit. PF08758. Cadherin_pro. 1 hit. [Graphical view]
PRINTS	PR00205. CADHERIN. PR01818. DESMOCADHERN. PR01820. DESMOCOLLIN.
SMART	SM00112. CA. 5 hits. [Graphical view]
PROSITE	PS00232. CADHERIN_1. 3 hits. PS50268. CADHERIN_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	284050.
SOURCE	Search...

Entry information

Entry name

DSC3_MOUSE

Accession

Primary (citable) accession number: P55850
Secondary accession number(s): O55110, O55122

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)