P55830 (RS3A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S3a

Alternative name(s):
C3 protein

Gene names

Name:	RpS3A
Synonyms:	C3, M(4)101
ORF Names:	CG2168

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for oogenesis; required for late follicle cell development. Ref.1
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S) By similarity.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Ubiquitously expressed in stage 8 embryos. During oogenesis, expression is located basally in somatic follicular epithelium and in the oocyte at the later stages. Ref.1
Developmental stage	Expressed both maternally and zygotically throughout all development. Ref.2
Disruption phenotype	Flies exhibit disappearance of the follicular cells of the ovary and abnormalities of the associated germline derivatives, leading to failure of egg production. Ref.1
Sequence similarities	Belongs to the ribosomal protein S3Ae family.
Sequence caution	The sequence AAL48571.1 differs from that shown. Reason: Frameshift at position 188. The sequence ACQ89821.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Oogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation Alternative splicing
Molecular function	Developmental protein Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle elongation Inferred from mutant phenotype PubMed 17412918. Source: FlyBase multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: InterPro
Cellular_component	lipid particle Inferred from direct assay PubMed 16979555. Source: FlyBase ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform A (identifier: P55830-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P55830-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: MAVGKNKGLS...GQRIASDYLK → MSKLRICFKPVKS

Note: Produced by alternative splicing.

Isoform D (identifier: P55830-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Note: Produced by alternative initiation at Met-106 of isoform A.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 268	267	40S ribosomal protein S3a HAMAP-Rule MF_03122		PRO_0000153529
Natural variations
	Alternative sequence	1 – 105	105	Missing in isoform D.		VSP_038422
	Alternative sequence	1 – 63	63	MAVGK…SDYLK → MSKLRICFKPVKS in isoform B.		VSP_038423
Experimental info
	Sequence conflict	62	1	L → F in CAA71201. Ref.1
	Sequence conflict	72	1	A → VP in CAA71201. Ref.1
	Sequence conflict	82	1	R → H in CAA71201. Ref.1
	Sequence conflict	160 – 161	2	QQ → HE in CAA71201. Ref.1
	Sequence conflict	169	1	A → SG in CAA71201. Ref.1
	Sequence conflict	253 – 261	9	VIDRPEGYE → PKSTALKVK in CAA71201. Ref.1
	Sequence conflict	267	1	A → S in CAA71201. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified March 2, 2010. Version 4. Checksum: EA407157F81955C6	FASTA	268	30,340
	10 20 30 40 50 60 MAVGKNKGLS KGGKKGGKKK VVDPFSRKDW YDVKAPNMFQ TRQIGKTLVN RTQGQRIASD 70 80 90 100 110 120 YLKGRVFEVS LADLQKDIDP ERSFRKFRLI AEDVQDRNVL CNFHGMDLTT DKYRSMVKKW 130 140 150 160 170 180 QTLIEAIVEA KTVDGYLLRV FCIGFTAKDQ QSQRKTCYAQ QSQVRKIRAR MTDIITNEVS 190 200 210 220 230 240 GADLKQLVNK LALDSIAKDI EKSCQRIYPL HDVYIRKVKV LKKPRFDVSK LLELHGDGGG 250 260 KSVEAVVSSE GAVIDRPEGY EPPVQEAV
	Isoform B [UniParc]. Checksum: EBE6F16029A4817D	FASTA	218	24,822
	10 20 30 40 50 60 MSKLRICFKP VKSGRVFEVS LADLQKDIDP ERSFRKFRLI AEDVQDRNVL CNFHGMDLTT 70 80 90 100 110 120 DKYRSMVKKW QTLIEAIVEA KTVDGYLLRV FCIGFTAKDQ QSQRKTCYAQ QSQVRKIRAR 130 140 150 160 170 180 MTDIITNEVS GADLKQLVNK LALDSIAKDI EKSCQRIYPL HDVYIRKVKV LKKPRFDVSK 190 200 210 LLELHGDGGG KSVEAVVSSE GAVIDRPEGY EPPVQEAV
	Isoform D [UniParc]. Checksum: DDF1BD80CE79E3C0	FASTA	163	18,386
	10 20 30 40 50 60 MDLTTDKYRS MVKKWQTLIE AIVEAKTVDG YLLRVFCIGF TAKDQQSQRK TCYAQQSQVR 70 80 90 100 110 120 KIRARMTDII TNEVSGADLK QLVNKLALDS IAKDIEKSCQ RIYPLHDVYI RKVKVLKKPR 130 140 150 160 FDVSKLLELH GDGGGKSVEA VVSSEGAVID RPEGYEPPVQ EAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Antisense suppression of the putative ribosomal protein S3A gene disrupts ovarian development in Drosophila melanogaster." Reynaud E., Bolshakov V.N., Barajas V.N., Kafatos F.C., Zurita M. Mol. Gen. Genet. 256:462-467(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: Oregon-R. Tissue: Embryo.
[2]	"Drosophila RpS3a, a novel minute gene situated between the segment polarity genes cubitus interruptus and dTCF." van Beest M., Mortin M., Clevers H. Nucleic Acids Res. 26:4471-4475(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DEVELOPMENTAL STAGE. Strain: Berkeley. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[6]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10115 mRNA. Translation: CAA71201.1. AF034971 mRNA. Translation: AAC62117.1. AE014135 Genomic DNA. Translation: AAF59372.1. AE014135 Genomic DNA. Translation: AAN06541.1. AE014135 Genomic DNA. Translation: AAN06542.1. AY070949 mRNA. Translation: AAL48571.1. Frameshift. BT083412 mRNA. Translation: ACQ89821.1. Different initiation.
RefSeq	NP_001245404.1. NM_001258475.2. NP_524618.1. NM_079879.4. NP_726518.1. NM_166714.4.
UniGene	Dm.31437. Dm.6768.
3D structure databases
ProteinModelPortal	P55830.
SMR	P55830. Positions 21-238.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55830. 2 interactions.
MINT	MINT-923192.
Proteomic databases
PaxDb	P55830.
PRIDE	P55830.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089175; FBpp0088242; FBgn0017545. FBtr0308296; FBpp0300615; FBgn0017545.
GeneID	43768.
KEGG	dme:Dmel_CG2168.
UCSC	CG2168-RB. d. melanogaster. CG2168-RD. d. melanogaster.
Organism-specific databases
CTD	6189.
FlyBase	FBgn0017545. RpS3A.
Phylogenomic databases
eggNOG	COG1890.
GeneTree	ENSGT00390000018433.
InParanoid	Q8IMB0.
KO	K02984.
OMA	RSLIHKW.
OrthoDB	EOG4DJHCB.
PhylomeDB	P55830.
Gene expression databases
Bgee	P55830.
GermOnline	CG2168. Drosophila melanogaster.
Family and domain databases
HAMAP	MF_03122. Ribosomal_S3Ae_euk.
InterPro	IPR027500. Ribosomal_S1/3_euk. IPR001593. Ribosomal_S3Ae. IPR018281. Ribosomal_S3Ae_CS. [Graphical view]
Pfam	PF01015. Ribosomal_S3Ae. 1 hit. [Graphical view]
PROSITE	PS01191. RIBOSOMAL_S3AE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPS3A. drosophila.
GenomeRNAi	43768.
NextBio	835682.

Entry information

Entry name

RS3A_DROME

Accession

Primary (citable) accession number: P55830
Secondary accession number(s): C4IXY1 , O44389, Q8IMA9, Q8IMB0, Q8SZD2, Q9V4A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 2, 2010
Last modified:	May 29, 2013
This is version 100 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project