Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

40S ribosomal protein S3a

Gene

RpS3A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for oogenesis; required for late follicle cell development.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • mitotic spindle elongation Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • oogenesis Source: FlyBase
  • translation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Differentiation, Oogenesis

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-DME-6791226. Major pathway of rRNA processing in the nucleolus.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-DME-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S3aUniRule annotation
Alternative name(s):
C3 protein
Gene namesi
Name:RpS3AUniRule annotation
Synonyms:C3, M(4)101
ORF Names:CG2168
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 4

Organism-specific databases

FlyBaseiFBgn0017545. RpS3A.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Flies exhibit disappearance of the follicular cells of the ovary and abnormalities of the associated germline derivatives, leading to failure of egg production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedUniRule annotation
Chaini2 – 26826740S ribosomal protein S3aPRO_0000153529Add
BLAST

Proteomic databases

PaxDbiP55830.
PRIDEiP55830.

Expressioni

Tissue specificityi

Ubiquitously expressed in stage 8 embryos. During oogenesis, expression is located basally in somatic follicular epithelium and in the oocyte at the later stages.1 Publication

Developmental stagei

Expressed both maternally and zygotically throughout all development.1 Publication

Gene expression databases

BgeeiFBgn0017545.
GenevisibleiP55830. DM.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S).UniRule annotation

Protein-protein interaction databases

BioGridi68606. 36 interactions.
IntActiP55830. 3 interactions.
MINTiMINT-923192.
STRINGi7227.FBpp0300615.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Welectron microscopy6.00AB1-268[»]
ProteinModelPortaliP55830.
SMRiP55830. Positions 19-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S3Ae family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1628. Eukaryota.
COG1890. LUCA.
GeneTreeiENSGT00390000018433.
InParanoidiP55830.
KOiK02984.
OMAiGQNAYTK.
OrthoDBiEOG091G0HTU.
PhylomeDBiP55830.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk. 1 hit.
InterProiIPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view]
PfamiPF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view]
SMARTiSM01397. Ribosomal_S3Ae. 1 hit.
[Graphical view]
PROSITEiPS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform A (identifier: P55830-1) [UniParc]FASTAAdd to basket
Also known as: F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVGKNKGLS KGGKKGGKKK VVDPFSRKDW YDVKAPNMFQ TRQIGKTLVN
60 70 80 90 100
RTQGQRIASD YLKGRVFEVS LADLQKDIDP ERSFRKFRLI AEDVQDRNVL
110 120 130 140 150
CNFHGMDLTT DKYRSMVKKW QTLIEAIVEA KTVDGYLLRV FCIGFTAKDQ
160 170 180 190 200
QSQRKTCYAQ QSQVRKIRAR MTDIITNEVS GADLKQLVNK LALDSIAKDI
210 220 230 240 250
EKSCQRIYPL HDVYIRKVKV LKKPRFDVSK LLELHGDGGG KSVEAVVSSE
260
GAVIDRPEGY EPPVQEAV
Length:268
Mass (Da):30,340
Last modified:March 2, 2010 - v4
Checksum:iEA407157F81955C6
GO
Isoform B (identifier: P55830-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: MAVGKNKGLS...GQRIASDYLK → MSKLRICFKPVKS

Note: Produced by alternative splicing.
Show »
Length:218
Mass (Da):24,822
Checksum:iEBE6F16029A4817D
GO

Sequence cautioni

The sequence AAL48571 differs from that shown. Reason: Frameshift at position 188. Curated
The sequence ACQ89821 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence ADR66775 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AEU08331 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621L → F in CAA71201 (PubMed:9393444).Curated
Sequence conflicti72 – 721A → VP in CAA71201 (PubMed:9393444).Curated
Sequence conflicti82 – 821R → H in CAA71201 (PubMed:9393444).Curated
Sequence conflicti152 – 1521S → C in ADR66775 (Ref. 6) Curated
Sequence conflicti160 – 1612QQ → HE in CAA71201 (PubMed:9393444).Curated
Sequence conflicti169 – 1691A → SG in CAA71201 (PubMed:9393444).Curated
Sequence conflicti253 – 2619VIDRPEGYE → PKSTALKVK in CAA71201 (PubMed:9393444).Curated
Sequence conflicti267 – 2671A → S in CAA71201 (PubMed:9393444).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6363MAVGK…SDYLK → MSKLRICFKPVKS in isoform B. 1 PublicationVSP_038423Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10115 mRNA. Translation: CAA71201.1.
AF034971 mRNA. Translation: AAC62117.1.
AE014135 Genomic DNA. Translation: AAF59372.1.
AE014135 Genomic DNA. Translation: AAN06541.1.
AE014135 Genomic DNA. Translation: AFH06764.1.
AY070949 mRNA. Translation: AAL48571.1. Frameshift.
BT083412 mRNA. Translation: ACQ89821.1. Different initiation.
BT125801 mRNA. Translation: ADR66775.1. Different initiation.
BT132838 mRNA. Translation: AEU08331.1. Different initiation.
RefSeqiNP_001245404.1. NM_001258475.2. [P55830-1]
NP_524618.1. NM_079879.4. [P55830-1]
NP_726518.1. NM_166714.4. [P55830-2]
UniGeneiDm.31437.
Dm.6768.

Genome annotation databases

EnsemblMetazoaiFBtr0089175; FBpp0088242; FBgn0017545. [P55830-1]
FBtr0308296; FBpp0300615; FBgn0017545. [P55830-1]
GeneIDi43768.
KEGGidme:Dmel_CG2168.

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10115 mRNA. Translation: CAA71201.1.
AF034971 mRNA. Translation: AAC62117.1.
AE014135 Genomic DNA. Translation: AAF59372.1.
AE014135 Genomic DNA. Translation: AAN06541.1.
AE014135 Genomic DNA. Translation: AFH06764.1.
AY070949 mRNA. Translation: AAL48571.1. Frameshift.
BT083412 mRNA. Translation: ACQ89821.1. Different initiation.
BT125801 mRNA. Translation: ADR66775.1. Different initiation.
BT132838 mRNA. Translation: AEU08331.1. Different initiation.
RefSeqiNP_001245404.1. NM_001258475.2. [P55830-1]
NP_524618.1. NM_079879.4. [P55830-1]
NP_726518.1. NM_166714.4. [P55830-2]
UniGeneiDm.31437.
Dm.6768.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Welectron microscopy6.00AB1-268[»]
ProteinModelPortaliP55830.
SMRiP55830. Positions 19-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68606. 36 interactions.
IntActiP55830. 3 interactions.
MINTiMINT-923192.
STRINGi7227.FBpp0300615.

Proteomic databases

PaxDbiP55830.
PRIDEiP55830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089175; FBpp0088242; FBgn0017545. [P55830-1]
FBtr0308296; FBpp0300615; FBgn0017545. [P55830-1]
GeneIDi43768.
KEGGidme:Dmel_CG2168.

Organism-specific databases

CTDi6189.
FlyBaseiFBgn0017545. RpS3A.

Phylogenomic databases

eggNOGiKOG1628. Eukaryota.
COG1890. LUCA.
GeneTreeiENSGT00390000018433.
InParanoidiP55830.
KOiK02984.
OMAiGQNAYTK.
OrthoDBiEOG091G0HTU.
PhylomeDBiP55830.

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-DME-6791226. Major pathway of rRNA processing in the nucleolus.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-DME-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiRpS3A. fly.
GenomeRNAii43768.
PROiP55830.

Gene expression databases

BgeeiFBgn0017545.
GenevisibleiP55830. DM.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk. 1 hit.
InterProiIPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view]
PfamiPF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view]
SMARTiSM01397. Ribosomal_S3Ae. 1 hit.
[Graphical view]
PROSITEiPS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS3A_DROME
AccessioniPrimary (citable) accession number: P55830
Secondary accession number(s): C4IXY1
, E4NKK4, H0RNB6, H9XVL9, O44389, Q8IMA9, Q8IMB0, Q8SZD2, Q9V4A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 2, 2010
Last modified: September 7, 2016
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.