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P55826

- PPOC_ARATH

UniProt

P55826 - PPOC_ARATH

Protein

Protoporphyrinogen oxidase 1, chloroplastic

Gene

PPOX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Enzyme regulationi

    Inhibited by acifluorfen.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi63 – 686FADBy similarity
    Nucleotide bindingi90 – 912FADBy similarity
    Nucleotide bindingi112 – 1154FADBy similarity
    Nucleotide bindingi511 – 5133FADBy similarity

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: TAIR

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
    2. porphyrin-containing compound biosynthetic process Source: TAIR
    3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT4G01690-MONOMER.
    ARA:GQT-2158-MONOMER.
    MetaCyc:AT4G01690-MONOMER.
    UniPathwayiUPA00251; UER00324.
    UPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase 1, chloroplastic (EC:1.3.3.4)
    Short name:
    PPO1
    Gene namesi
    Name:PPOX1
    Synonyms:HEMG1, PPOP1
    Ordered Locus Names:At4g01690
    ORF Names:T15B16.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G01690.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. chloroplast thylakoid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333ChloroplastSequence AnalysisAdd
    BLAST
    Chaini34 – 537504Protoporphyrinogen oxidase 1, chloroplasticPRO_0000013325Add
    BLAST

    Proteomic databases

    PaxDbiP55826.
    PRIDEiP55826.

    Expressioni

    Tissue specificityi

    Expressed at high levels in the leaves and at low levels in the roots and floral buds.

    Gene expression databases

    ArrayExpressiP55826.
    GenevestigatoriP55826.

    Structurei

    3D structure databases

    ProteinModelPortaliP55826.
    SMRiP55826. Positions 58-524.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1232.
    HOGENOMiHOG000269479.
    InParanoidiP55826.
    KOiK00231.
    OMAiGQFKQFR.
    PhylomeDBiP55826.

    Family and domain databases

    Gene3Di3.90.660.20. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P55826-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELSLLRPTT QSLLPSFSKP NLRLNVYKPL RLRCSVAGGP TVGSSKIEGG    50
    GGTTITTDCV IVGGGISGLC IAQALATKHP DAAPNLIVTE AKDRVGGNII 100
    TREENGFLWE EGPNSFQPSD PMLTMVVDSG LKDDLVLGDP TAPRFVLWNG 150
    KLRPVPSKLT DLPFFDLMSI GGKIRAGFGA LGIRPSPPGR EESVEEFVRR 200
    NLGDEVFERL IEPFCSGVYA GDPSKLSMKA AFGKVWKLEQ NGGSIIGGTF 250
    KAIQERKNAP KAERDPRLPK PQGQTVGSFR KGLRMLPEAI SARLGSKVKL 300
    SWKLSGITKL ESGGYNLTYE TPDGLVSVQS KSVVMTVPSH VASGLLRPLS 350
    ESAANALSKL YYPPVAAVSI SYPKEAIRTE CLIDGELKGF GQLHPRTQGV 400
    ETLGTIYSSS LFPNRAPPGR ILLLNYIGGS TNTGILSKSE GELVEAVDRD 450
    LRKMLIKPNS TDPLKLGVRV WPQAIPQFLV GHFDILDTAK SSLTSSGYEG 500
    LFLGGNYVAG VALGRCVEGA YETAIEVNNF MSRYAYK 537
    Length:537
    Mass (Da):57,695
    Last modified:November 1, 1997 - v1
    Checksum:iC125B0EE1D3187D5
    GO
    Isoform 2 (identifier: P55826-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         447-477: Missing.

    Note: Derived from EST data.

    Show »
    Length:506
    Mass (Da):54,128
    Checksum:iEA7DF617A0C8504E
    GO

    Sequence cautioni

    The sequence AAC72870.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti302 – 3021W → C in AAK62389. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei447 – 47731Missing in isoform 2. CuratedVSP_046547Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83139 mRNA. Translation: BAA11820.1.
    AF104919 Genomic DNA. Translation: AAC72870.1. Sequence problems.
    AL161492 Genomic DNA. Translation: CAB77739.1.
    CP002687 Genomic DNA. Translation: AEE82064.1.
    CP002687 Genomic DNA. Translation: AEE82065.1.
    AF386944 mRNA. Translation: AAK62389.1.
    BT020418 mRNA. Translation: AAV97809.1.
    BT020535 mRNA. Translation: AAW70381.1.
    PIRiG85021.
    T02005.
    RefSeqiNP_192078.1. NM_116399.3. [P55826-1]
    NP_849283.1. NM_178952.2. [P55826-2]
    UniGeneiAt.19758.
    At.70176.

    Genome annotation databases

    EnsemblPlantsiAT4G01690.1; AT4G01690.1; AT4G01690. [P55826-1]
    GeneIDi827991.
    KEGGiath:AT4G01690.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83139 mRNA. Translation: BAA11820.1 .
    AF104919 Genomic DNA. Translation: AAC72870.1 . Sequence problems.
    AL161492 Genomic DNA. Translation: CAB77739.1 .
    CP002687 Genomic DNA. Translation: AEE82064.1 .
    CP002687 Genomic DNA. Translation: AEE82065.1 .
    AF386944 mRNA. Translation: AAK62389.1 .
    BT020418 mRNA. Translation: AAV97809.1 .
    BT020535 mRNA. Translation: AAW70381.1 .
    PIRi G85021.
    T02005.
    RefSeqi NP_192078.1. NM_116399.3. [P55826-1 ]
    NP_849283.1. NM_178952.2. [P55826-2 ]
    UniGenei At.19758.
    At.70176.

    3D structure databases

    ProteinModelPortali P55826.
    SMRi P55826. Positions 58-524.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P55826.
    PRIDEi P55826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G01690.1 ; AT4G01690.1 ; AT4G01690 . [P55826-1 ]
    GeneIDi 827991.
    KEGGi ath:AT4G01690.

    Organism-specific databases

    TAIRi AT4G01690.

    Phylogenomic databases

    eggNOGi COG1232.
    HOGENOMi HOG000269479.
    InParanoidi P55826.
    KOi K00231.
    OMAi GQFKQFR.
    PhylomeDBi P55826.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .
    UPA00668 .
    BioCyci ARA:AT4G01690-MONOMER.
    ARA:GQT-2158-MONOMER.
    MetaCyc:AT4G01690-MONOMER.

    Gene expression databases

    ArrayExpressi P55826.
    Genevestigatori P55826.

    Family and domain databases

    Gene3Di 3.90.660.20. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a cDNA that encodes protoporphyrinogen oxidase of Arabidopsis thaliana."
      Narita S., Tanaka R., Ito T., Okada K., Taketani S., Inokuchi H.
      Gene 182:169-175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Arabidopsis ORF clones."
      Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

    Entry informationi

    Entry nameiPPOC_ARATH
    AccessioniPrimary (citable) accession number: P55826
    Secondary accession number(s): F4JG44
    , Q5M730, Q94F25, Q9ZSI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3