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P55826

- PPOC_ARATH

UniProt

P55826 - PPOC_ARATH

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Protein

Protoporphyrinogen oxidase 1, chloroplastic

Gene

PPOX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Enzyme regulationi

Inhibited by acifluorfen.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 686FADBy similarity
Nucleotide bindingi90 – 912FADBy similarity
Nucleotide bindingi112 – 1154FADBy similarity
Nucleotide bindingi511 – 5133FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: TAIR

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. porphyrin-containing compound biosynthetic process Source: TAIR
  3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT4G01690-MONOMER.
ARA:GQT-2158-MONOMER.
MetaCyc:AT4G01690-MONOMER.
UniPathwayiUPA00251; UER00324.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase 1, chloroplastic (EC:1.3.3.4)
Short name:
PPO1
Gene namesi
Name:PPOX1
Synonyms:HEMG1, PPOP1
Ordered Locus Names:At4g01690
ORF Names:T15B16.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G01690.

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333ChloroplastSequence AnalysisAdd
BLAST
Chaini34 – 537504Protoporphyrinogen oxidase 1, chloroplasticPRO_0000013325Add
BLAST

Proteomic databases

PaxDbiP55826.
PRIDEiP55826.

Expressioni

Tissue specificityi

Expressed at high levels in the leaves and at low levels in the roots and floral buds.

Gene expression databases

GenevestigatoriP55826.

Structurei

3D structure databases

ProteinModelPortaliP55826.
SMRiP55826. Positions 58-524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1232.
HOGENOMiHOG000269479.
InParanoidiP55826.
KOiK00231.
OMAiGQFKQFR.
PhylomeDBiP55826.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P55826-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELSLLRPTT QSLLPSFSKP NLRLNVYKPL RLRCSVAGGP TVGSSKIEGG
60 70 80 90 100
GGTTITTDCV IVGGGISGLC IAQALATKHP DAAPNLIVTE AKDRVGGNII
110 120 130 140 150
TREENGFLWE EGPNSFQPSD PMLTMVVDSG LKDDLVLGDP TAPRFVLWNG
160 170 180 190 200
KLRPVPSKLT DLPFFDLMSI GGKIRAGFGA LGIRPSPPGR EESVEEFVRR
210 220 230 240 250
NLGDEVFERL IEPFCSGVYA GDPSKLSMKA AFGKVWKLEQ NGGSIIGGTF
260 270 280 290 300
KAIQERKNAP KAERDPRLPK PQGQTVGSFR KGLRMLPEAI SARLGSKVKL
310 320 330 340 350
SWKLSGITKL ESGGYNLTYE TPDGLVSVQS KSVVMTVPSH VASGLLRPLS
360 370 380 390 400
ESAANALSKL YYPPVAAVSI SYPKEAIRTE CLIDGELKGF GQLHPRTQGV
410 420 430 440 450
ETLGTIYSSS LFPNRAPPGR ILLLNYIGGS TNTGILSKSE GELVEAVDRD
460 470 480 490 500
LRKMLIKPNS TDPLKLGVRV WPQAIPQFLV GHFDILDTAK SSLTSSGYEG
510 520 530
LFLGGNYVAG VALGRCVEGA YETAIEVNNF MSRYAYK
Length:537
Mass (Da):57,695
Last modified:November 1, 1997 - v1
Checksum:iC125B0EE1D3187D5
GO
Isoform 2 (identifier: P55826-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     447-477: Missing.

Note: Derived from EST data.

Show »
Length:506
Mass (Da):54,128
Checksum:iEA7DF617A0C8504E
GO

Sequence cautioni

The sequence AAC72870.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021W → C in AAK62389. (PubMed:14593172)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei447 – 47731Missing in isoform 2. CuratedVSP_046547Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83139 mRNA. Translation: BAA11820.1.
AF104919 Genomic DNA. Translation: AAC72870.1. Sequence problems.
AL161492 Genomic DNA. Translation: CAB77739.1.
CP002687 Genomic DNA. Translation: AEE82064.1.
CP002687 Genomic DNA. Translation: AEE82065.1.
AF386944 mRNA. Translation: AAK62389.1.
BT020418 mRNA. Translation: AAV97809.1.
BT020535 mRNA. Translation: AAW70381.1.
PIRiG85021.
T02005.
RefSeqiNP_192078.1. NM_116399.3. [P55826-1]
NP_849283.1. NM_178952.2. [P55826-2]
UniGeneiAt.19758.
At.70176.

Genome annotation databases

EnsemblPlantsiAT4G01690.1; AT4G01690.1; AT4G01690. [P55826-1]
GeneIDi827991.
KEGGiath:AT4G01690.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83139 mRNA. Translation: BAA11820.1 .
AF104919 Genomic DNA. Translation: AAC72870.1 . Sequence problems.
AL161492 Genomic DNA. Translation: CAB77739.1 .
CP002687 Genomic DNA. Translation: AEE82064.1 .
CP002687 Genomic DNA. Translation: AEE82065.1 .
AF386944 mRNA. Translation: AAK62389.1 .
BT020418 mRNA. Translation: AAV97809.1 .
BT020535 mRNA. Translation: AAW70381.1 .
PIRi G85021.
T02005.
RefSeqi NP_192078.1. NM_116399.3. [P55826-1 ]
NP_849283.1. NM_178952.2. [P55826-2 ]
UniGenei At.19758.
At.70176.

3D structure databases

ProteinModelPortali P55826.
SMRi P55826. Positions 58-524.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL2366479.

Proteomic databases

PaxDbi P55826.
PRIDEi P55826.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G01690.1 ; AT4G01690.1 ; AT4G01690 . [P55826-1 ]
GeneIDi 827991.
KEGGi ath:AT4G01690.

Organism-specific databases

TAIRi AT4G01690.

Phylogenomic databases

eggNOGi COG1232.
HOGENOMi HOG000269479.
InParanoidi P55826.
KOi K00231.
OMAi GQFKQFR.
PhylomeDBi P55826.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
UPA00668 .
BioCyci ARA:AT4G01690-MONOMER.
ARA:GQT-2158-MONOMER.
MetaCyc:AT4G01690-MONOMER.

Gene expression databases

Genevestigatori P55826.

Family and domain databases

Gene3Di 3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a cDNA that encodes protoporphyrinogen oxidase of Arabidopsis thaliana."
    Narita S., Tanaka R., Ito T., Okada K., Taketani S., Inokuchi H.
    Gene 182:169-175(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiPPOC_ARATH
AccessioniPrimary (citable) accession number: P55826
Secondary accession number(s): F4JG44
, Q5M730, Q94F25, Q9ZSI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3