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Protein

Protoporphyrinogen oxidase 1, chloroplastic

Gene

PPOX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Enzyme regulationi

Inhibited by acifluorfen.1 Publication

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial (PPOX2), Protoporphyrinogen oxidase (AXX17_At5g13680), Protoporphyrinogen oxidase (AXX17_At4g02130), Protoporphyrinogen oxidase 1, chloroplastic (PPOX1), Protoporphyrinogen oxidase (HEMG2), Protoporphyrinogen oxidase (HEMG2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 68FADBy similarity6
Nucleotide bindingi90 – 91FADBy similarity2
Nucleotide bindingi112 – 115FADBy similarity4
Nucleotide bindingi511 – 513FADBy similarity3

GO - Molecular functioni

  • oxygen-dependent protoporphyrinogen oxidase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT4G01690-MONOMER.
MetaCyc:AT4G01690-MONOMER.
UniPathwayiUPA00251; UER00324.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase 1, chloroplastic (EC:1.3.3.4)
Short name:
PPO1
Gene namesi
Name:PPOX1
Synonyms:HEMG1, PPOP1
Ordered Locus Names:At4g01690
ORF Names:T15B16.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G01690.
TAIRilocus:2133397. AT4G01690.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast thylakoid Source: TAIR

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2366479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34ChloroplastCombined sourcesAdd BLAST34
ChainiPRO_000001332535 – 537Protoporphyrinogen oxidase 1, chloroplasticAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP55826.
PRIDEiP55826.

PTM databases

iPTMnetiP55826.

Expressioni

Tissue specificityi

Expressed at high levels in the leaves and at low levels in the roots and floral buds.

Gene expression databases

ExpressionAtlasiP55826. baseline and differential.
GenevisibleiP55826. AT.

Interactioni

Protein-protein interaction databases

BioGridi13282. 3 interactors.
STRINGi3702.AT4G01690.1.

Structurei

3D structure databases

ProteinModelPortaliP55826.
SMRiP55826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1276. Eukaryota.
COG1232. LUCA.
HOGENOMiHOG000269479.
InParanoidiP55826.
KOiK00231.
OMAiPMICGIC.
OrthoDBiEOG09360IRW.
PhylomeDBiP55826.

Family and domain databases

InterProiView protein in InterPro
IPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR004572. Protoporphyrinogen_oxidase.
PfamiView protein in Pfam
PF01593. Amino_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P55826-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELSLLRPTT QSLLPSFSKP NLRLNVYKPL RLRCSVAGGP TVGSSKIEGG
60 70 80 90 100
GGTTITTDCV IVGGGISGLC IAQALATKHP DAAPNLIVTE AKDRVGGNII
110 120 130 140 150
TREENGFLWE EGPNSFQPSD PMLTMVVDSG LKDDLVLGDP TAPRFVLWNG
160 170 180 190 200
KLRPVPSKLT DLPFFDLMSI GGKIRAGFGA LGIRPSPPGR EESVEEFVRR
210 220 230 240 250
NLGDEVFERL IEPFCSGVYA GDPSKLSMKA AFGKVWKLEQ NGGSIIGGTF
260 270 280 290 300
KAIQERKNAP KAERDPRLPK PQGQTVGSFR KGLRMLPEAI SARLGSKVKL
310 320 330 340 350
SWKLSGITKL ESGGYNLTYE TPDGLVSVQS KSVVMTVPSH VASGLLRPLS
360 370 380 390 400
ESAANALSKL YYPPVAAVSI SYPKEAIRTE CLIDGELKGF GQLHPRTQGV
410 420 430 440 450
ETLGTIYSSS LFPNRAPPGR ILLLNYIGGS TNTGILSKSE GELVEAVDRD
460 470 480 490 500
LRKMLIKPNS TDPLKLGVRV WPQAIPQFLV GHFDILDTAK SSLTSSGYEG
510 520 530
LFLGGNYVAG VALGRCVEGA YETAIEVNNF MSRYAYK
Length:537
Mass (Da):57,695
Last modified:November 1, 1997 - v1
Checksum:iC125B0EE1D3187D5
GO
Isoform 2 (identifier: P55826-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     447-477: Missing.

Note: Derived from EST data.
Show »
Length:506
Mass (Da):54,128
Checksum:iEA7DF617A0C8504E
GO

Sequence cautioni

The sequence AAC72870 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302W → C in AAK62389 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046547447 – 477Missing in isoform 2. CuratedAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83139 mRNA. Translation: BAA11820.1.
AF104919 Genomic DNA. Translation: AAC72870.1. Sequence problems.
AL161492 Genomic DNA. Translation: CAB77739.1.
CP002687 Genomic DNA. Translation: AEE82064.1.
CP002687 Genomic DNA. Translation: AEE82065.1.
AF386944 mRNA. Translation: AAK62389.1.
BT020418 mRNA. Translation: AAV97809.1.
BT020535 mRNA. Translation: AAW70381.1.
PIRiG85021.
T02005.
RefSeqiNP_192078.1. NM_116399.4. [P55826-1]
NP_849283.1. NM_178952.2. [P55826-2]
UniGeneiAt.19758.
At.70176.

Genome annotation databases

EnsemblPlantsiAT4G01690.1; AT4G01690.1; AT4G01690. [P55826-1]
AT4G01690.2; AT4G01690.2; AT4G01690. [P55826-2]
GeneIDi827991.
GrameneiAT4G01690.1; AT4G01690.1; AT4G01690.
AT4G01690.2; AT4G01690.2; AT4G01690.
KEGGiath:AT4G01690.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPPOC_ARATH
AccessioniPrimary (citable) accession number: P55826
Secondary accession number(s): F4JG44
, Q5M730, Q94F25, Q9ZSI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families