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P55824

- FAF_DROME

UniProt

P55824 - FAF_DROME

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Protein

Probable ubiquitin carboxyl-terminal hydrolase FAF

Gene

faf

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for eye and embryo development, and plays a role in compound eye assembly and oogenesis respectively. In the larval eye disks, cells outside the assembling facets require this protein for short-range cell interactions that prevent the mystery cells from becoming photoreceptors. It is also required for nuclear migration and cellularization in early embryogenesis and could play a role in pole cell determination, development or function.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1677 – 16771NucleophilePROSITE-ProRule annotation
Active sitei1986 – 19861Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  1. cellularization Source: FlyBase
  2. compound eye development Source: FlyBase
  3. defense response to Gram-negative bacterium Source: FlyBase
  4. dorsal/ventral axis specification Source: FlyBase
  5. endocytosis Source: FlyBase
  6. germ cell migration Source: FlyBase
  7. mystery cell differentiation Source: FlyBase
  8. negative regulation of innate immune response Source: FlyBase
  9. negative regulation of proteolysis Source: FlyBase
  10. nuclear migration Source: FlyBase
  11. oogenesis Source: UniProtKB-KW
  12. protein deubiquitination Source: FlyBase
  13. ubiquitin-dependent protein catabolic process Source: FlyBase
  14. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Differentiation, Oogenesis, Sensory transduction, Ubl conjugation pathway, Vision

Enzyme and pathway databases

ReactomeiREACT_181669. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Protein family/group databases

MEROPSiC19.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase FAF (EC:3.4.19.12)
Alternative name(s):
Protein fat facets
Ubiquitin thioesterase FAF
Ubiquitin-specific-processing protease FAF
Short name:
Deubiquitinating enzyme FAF
Gene namesi
Name:faf
ORF Names:CG1945
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0005632. faf.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27782778Probable ubiquitin carboxyl-terminal hydrolase FAFPRO_0000080688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei924 – 9241Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP55824.
PRIDEiP55824.

Expressioni

Tissue specificityi

Eye disks and ovaries.1 Publication

Developmental stagei

Expressed both maternally and zygotically.

Gene expression databases

BgeeiP55824.
ExpressionAtlasiP55824. differential.

Interactioni

Protein-protein interaction databases

BioGridi68590. 15 interactions.

Structurei

3D structure databases

ProteinModelPortaliP55824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1668 – 2062395USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119158.
InParanoidiP55824.
KOiK11840.
OMAiMAQEQFF.
OrthoDBiEOG722J7K.
PhylomeDBiP55824.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: P55824-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTFDTRRHTT GQPGSTAPSS SSSTTSTTTT TTSPAQSAGS GSGIGTGTGT
60 70 80 90 100
VANSSLPGGG SGSLDGNQDQ QPATDSQSSD DVAASLSANS VDSTITIVPP
110 120 130 140 150
EKLISSFPTT KLRSLTQKIS NPRWVVPVLP EQELEVLLNA AIELTQAGVD
160 170 180 190 200
HDCEPCVEFY RNGLSTSFAK ILTDEAVNSW KNNIHHCILV SCGKLLHLIA
210 220 230 240 250
IHMQRDNPYL LDLLAIVFDP ENKFNTFNAG RQPECFAAPD YIWGQLDSNK
260 270 280 290 300
MYARPPPEPK NARGWLVDLI NRFGQLGGFD NLLERFNIGL ELLKRNQNKC
310 320 330 340 350
TGKNISVEGR VENGAQDNRL TLALIHSLLR PFGQCYELLM PATIAKYFMP
360 370 380 390 400
TWNVVLDLLD SFTDEELKRE VKPEGRNDYI NGIVKSARLL ASRLTGQEEL
410 420 430 440 450
IRDLEMFRLK MILRLLQVSS FNGKMNALNE INKVLSSVAY FSHRSQPLPH
460 470 480 490 500
CMPEDEMDWL TADRMAQWIK SSDVLGVVLK DSLHQPQYVE KLEKIIRFLI
510 520 530 540 550
KEQALTLDDL DAVWRAQAGK HEAIVKNVHD LLAKLAWDFT PEQLDHLFEA
560 570 580 590 600
FQASMTTANK RQRERLLELI RRLAEDDKNG VMAQKVLKLF WTLAHSQEVP
610 620 630 640 650
PEVLDQALGA HVKILDYSCS QERDAQKTIW LDKCVDELKS GDGWVLPALR
660 670 680 690 700
LIRDICCLYD TTTNHAQRTQ TSTNRQQVIE RLQNDYSLVI LVTNSLTAYM
710 720 730 740 750
EKVRQMVTDS PGLDATRILI DGRFPHHVQI AERLEFLKFL LKDGQLWLCA
760 770 780 790 800
DQAKQIWHCL AVNAVFPADR EECFRWFGKL MGEEPDLDPG INKDFFENNI
810 820 830 840 850
LQLDPHLLTE SGIKCFERFF KAVNSKEDKL KAIHRGYMLD NEDLIGKDYL
860 870 880 890 900
WRVITTGGEE IASKAIDLLK EVSTALGPRL QENIAEFHEM FIGECCSRLR
910 920 930 940 950
THYGNIVILG KTQLQEELDA PDQSDNTNDE SKDSKMRFIE AEKMCRILKV
960 970 980 990 1000
LQEYVKECDR SFSGDRVHLP LSRVTRGKNT ILYIRFQNPG RSIDDMEIVT
1010 1020 1030 1040 1050
HSNETMAAFK RNLLKRIKGT STANIKVDLF YANDEMIGVS DEINPLYQYT
1060 1070 1080 1090 1100
IRDKMNLTAK LTPVGTGLAS SPDSSSDSST GSPPRPCPDM QRVESESTLP
1110 1120 1130 1140 1150
GVIISQNYQY TEFFLKLYQL GSDLEHGRLR DSAKVLLHLL PCDRQTIRQL
1160 1170 1180 1190 1200
KIMCKVPKAA VTVAVTGDKI AKDEEEKLYP TEQAGIEDEE EHCTPEQMFL
1210 1220 1230 1240 1250
HPTPAQVLYN LSVLHGLLIP ALDPLGESAL LVQSAWMHSG CAHFVLELLT
1260 1270 1280 1290 1300
KNNFLPSADM HTKRASFQCV LRLAKLFLYI VGSVLSRVGD EPMICDLDNG
1310 1320 1330 1340 1350
SRSQVDILKQ NFSTMPSSSQ GTLRAISAKL AVILAREMLS ASPEGDRCRT
1360 1370 1380 1390 1400
LFSSTLQWSC PDISTIKAVV QLAWASSCGN LQALGNSSGD FEDEVIVPDG
1410 1420 1430 1440 1450
QDFSMCKEAL EVLTISFILN PSANEALTSD PNWPKFITSI VLKNPLRHVR
1460 1470 1480 1490 1500
QVASEQLFLA STYCAGDRRP FVYMVNLLVG ALKTLVPQYE STCAEFFSVL
1510 1520 1530 1540 1550
CRTLSYGCIY NWPLQISEGL LGDEIKWLQR IRENVHATGD TQVHEELLEG
1560 1570 1580 1590 1600
HLCLAKELMF FLGADSKAQL NELIHELIDD FLFTASREFL HLRRHGSLRQ
1610 1620 1630 1640 1650
DTVPPPVCRS PHTIAAACDL LIALCQLCVP NMKLLTNTLI DFVCTDTDPL
1660 1670 1680 1690 1700
REWDYLPPVG ARPTKGFCGL KNAGATCYMN SVLQQLYMVP AVRVGILRAH
1710 1720 1730 1740 1750
GAATTDGEDF SGDSDLTGGG LGSALFSGPA SALVSLPSSS STIEDGLHDV
1760 1770 1780 1790 1800
RKNYHVVILK HVQAIFAHLG HSALQYYVPR GLWTHFKLLG EPVNLREQQD
1810 1820 1830 1840 1850
AVEFFMSLLE SLDEGLKALG QPQLMNATLG GSFSDQKICQ ECPHRYSKEE
1860 1870 1880 1890 1900
PFSVFSVDIR NHSSLTESLE QYVKGELLEG ADAYHCDKCD KKVVTVKRVC
1910 1920 1930 1940 1950
VKKLPPVLAI QLKRFEYDYE RVCAIKFNDY FEFPRILDME PYTVSGLAKL
1960 1970 1980 1990 2000
EGEVVEVGDN CQTNVETTKY ELTGIVVHSG QASGGHYFSY ILSKNPANGK
2010 2020 2030 2040 2050
CQWYKFDDGE VTECKMHEDE EMKAECFGGE YMGETYDNNL KRMQYRRQKR
2060 2070 2080 2090 2100
WWNAYMLFYT RCDQTPVQYE PSVEQLSLAE SRNMVLPLPK PIERSVRHQN
2110 2120 2130 2140 2150
IRFLHSRSIF SVEFFNFIKK LVSCNLLSAR SNKITPAAEE LSLLGVQLAS
2160 2170 2180 2190 2200
QFLFHTGFRT KKSLRGPVME WYDALSHHIR SSALVRKWFA NHALLSPPSR
2210 2220 2230 2240 2250
LGEYILMAPS PDVRTVFVKL VVFFCHFAIN DEPLTGYDGA NLCEQVLISV
2260 2270 2280 2290 2300
LRLLKSEAAD YGKHLPHYFS LFSMYVGLGT REKQQLLRLN VPLQFIQVAL
2310 2320 2330 2340 2350
DDGPGPAIKY QYPEFSKLHQ VVSHLIRCSD VSEKCQSSNQ NARPLSNPFK
2360 2370 2380 2390 2400
DPNVAHEELT PLSTECMDLL FNRTGYIKKV IEDTNVGDEG LKLLQYCSWE
2410 2420 2430 2440 2450
NPHFSRAVLT ELLWQCGFAY CHDMRHHTDL LLNILLIDDS WQHHRIHNAL
2460 2470 2480 2490 2500
NGVAEEREGL LETIQRAKTH YQKRAYQIIK CLTQLFHKSP IALQMLHTNS
2510 2520 2530 2540 2550
NITRHWSIAV EWLQGELDRQ RGIGCQYNSY SWSPPAQSND NTNGYMLERS
2560 2570 2580 2590 2600
QSAKNTWSMA FELCPDEVSE KTDENNEPNL ETNMDENKSE PVAQPGGVLE
2610 2620 2630 2640 2650
GSTGGTEQLP ENKTPTTSSP STAAWPARGD SNAIPRLSRQ LFGAYTSTGS
2660 2670 2680 2690 2700
GSTSGGSAPT SALTTTAGSG ANSETESSAQ ETTGETTING LTNSLDQMEI
2710 2720 2730 2740 2750
TAKKKCRRVI IKKLVESKDE EDATTATTAA TTEVTTSPAT AIATAATLEP
2760 2770
AGMSELTTMV EKNLIISQEN PQAKSSLQ
Length:2,778
Mass (Da):311,143
Last modified:November 28, 2002 - v2
Checksum:iFFB90438BA53A02B
GO
Isoform 3 (identifier: P55824-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2705-2778: KCRRVIIKKL...ENPQAKSSLQ → VTRANNV

Show »
Length:2,711
Mass (Da):304,080
Checksum:i0D6319FFCC88EA5C
GO

Sequence cautioni

Isoform 1 : The sequence AAF01345.1 differs from that shown. Reason: Frameshift at positions 2742 and 2750.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2341E → D in AAF01345. (PubMed:1295747)Curated
Sequence conflicti234 – 2341E → D in AAF01346. (PubMed:1295747)Curated
Sequence conflicti2725 – 27251T → S in AAF01345. (PubMed:1295747)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2705 – 277874KCRRV…KSSLQ → VTRANNV in isoform 3. 1 PublicationVSP_005269Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04959 mRNA. Translation: AAF01345.1. Frameshift.
L04958 mRNA. Translation: AAF01346.1.
L04960 Genomic DNA. Translation: AAF01347.1.
L04960 Genomic DNA. Translation: AAF01348.1.
AE014297 Genomic DNA. Translation: AAF57198.1.
AE014297 Genomic DNA. Translation: AAN14291.1.
AF145677 mRNA. Translation: AAD38652.1.
PIRiB49132.
RefSeqiNP_524612.2. NM_079873.5. [P55824-1]
NP_733455.1. NM_170576.3. [P55824-3]
UniGeneiDm.3133.

Genome annotation databases

EnsemblMetazoaiFBtr0085843; FBpp0085202; FBgn0005632. [P55824-1]
GeneIDi43749.
KEGGidme:Dmel_CG1945.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04959 mRNA. Translation: AAF01345.1 . Frameshift.
L04958 mRNA. Translation: AAF01346.1 .
L04960 Genomic DNA. Translation: AAF01347.1 .
L04960 Genomic DNA. Translation: AAF01348.1 .
AE014297 Genomic DNA. Translation: AAF57198.1 .
AE014297 Genomic DNA. Translation: AAN14291.1 .
AF145677 mRNA. Translation: AAD38652.1 .
PIRi B49132.
RefSeqi NP_524612.2. NM_079873.5. [P55824-1 ]
NP_733455.1. NM_170576.3. [P55824-3 ]
UniGenei Dm.3133.

3D structure databases

ProteinModelPortali P55824.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 68590. 15 interactions.

Protein family/group databases

MEROPSi C19.007.

Proteomic databases

PaxDbi P55824.
PRIDEi P55824.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0085843 ; FBpp0085202 ; FBgn0005632 . [P55824-1 ]
GeneIDi 43749.
KEGGi dme:Dmel_CG1945.

Organism-specific databases

FlyBasei FBgn0005632. faf.

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00760000119158.
InParanoidi P55824.
KOi K11840.
OMAi MAQEQFF.
OrthoDBi EOG722J7K.
PhylomeDBi P55824.

Enzyme and pathway databases

Reactomei REACT_181669. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Miscellaneous databases

ChiTaRSi faf. drosophila.
GenomeRNAii 43749.
NextBioi 835588.
PROi P55824.

Gene expression databases

Bgeei P55824.
ExpressionAtlasi P55824. differential.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 6 hits.
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The fat facets gene is required for Drosophila eye and embryo development."
    Fischer-Vize J.A., Rubin G.M., Lehmann R.
    Development 116:985-1000(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY.
    Tissue: Eye imaginal disk.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1089-2778 (ISOFORM 1).
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-924, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiFAF_DROME
AccessioniPrimary (citable) accession number: P55824
Secondary accession number(s): Q9V9T6, Q9Y0Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2002
Last modified: October 29, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3