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P55824 (FAF_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin carboxyl-terminal hydrolase FAF

EC=3.4.19.12
Alternative name(s):
Protein fat facets
Ubiquitin thioesterase FAF
Ubiquitin-specific-processing protease FAF
Short name=Deubiquitinating enzyme FAF
Gene names
Name:faf
ORF Names:CG1945
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length2778 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for eye and embryo development, and plays a role in compound eye assembly and oogenesis respectively. In the larval eye disks, cells outside the assembling facets require this protein for short-range cell interactions that prevent the mystery cells from becoming photoreceptors. It is also required for nuclear migration and cellularization in early embryogenesis and could play a role in pole cell determination, development or function.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Tissue specificity

Eye disks and ovaries. Ref.1

Developmental stage

Expressed both maternally and zygotically.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

Isoform 1: The sequence AAF01345.1 differs from that shown. Reason: Frameshift at positions 2742 and 2750.

Ontologies

Keywords
   Biological processDifferentiation
Oogenesis
Sensory transduction
Ubl conjugation pathway
Vision
   Coding sequence diversityAlternative splicing
   Molecular functionDevelopmental protein
Hydrolase
Protease
Thiol protease
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellularization

Inferred from mutant phenotype Ref.1. Source: FlyBase

compound eye development

Inferred from mutant phenotype Ref.1. Source: FlyBase

defense response to Gram-negative bacterium

Inferred from mutant phenotype PubMed 19520911. Source: FlyBase

dorsal/ventral axis specification

Inferred from mutant phenotype PubMed 22745309. Source: FlyBase

embryo development

Inferred from mutant phenotype Ref.1. Source: FlyBase

endocytosis

Non-traceable author statement PubMed 11697879. Source: FlyBase

germ cell migration

Traceable author statement PubMed 12814944. Source: FlyBase

mystery cell differentiation

Inferred from mutant phenotype Ref.1. Source: FlyBase

negative regulation of innate immune response

Inferred from mutant phenotype PubMed 19520911. Source: FlyBase

negative regulation of proteolysis

Non-traceable author statement PubMed 11697879. Source: FlyBase

nuclear migration

Inferred from mutant phenotype Ref.1. Source: FlyBase

oogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

protein deubiquitination

Inferred from direct assay PubMed 8525378. Source: FlyBase

ubiquitin-dependent protein catabolic process

Inferred from genetic interaction PubMed 8525378. Source: FlyBase

visual perception

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay Ref.1. Source: FlyBase

   Molecular_functionubiquitin-specific protease activity

Traceable author statement PubMed 10570463. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P55824-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 3 (identifier: P55824-3)

The sequence of this isoform differs from the canonical sequence as follows:
     2705-2778: KCRRVIIKKL...ENPQAKSSLQ → VTRANNV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 27782778Probable ubiquitin carboxyl-terminal hydrolase FAF
PRO_0000080688

Regions

Domain1668 – 2062395USP

Sites

Active site16771Nucleophile By similarity
Active site19861Proton acceptor By similarity

Amino acid modifications

Modified residue9241Phosphoserine Ref.5

Natural variations

Alternative sequence2705 – 277874KCRRV…KSSLQ → VTRANNV in isoform 3.
VSP_005269

Experimental info

Sequence conflict2341E → D in AAF01345. Ref.1
Sequence conflict2341E → D in AAF01346. Ref.1
Sequence conflict27251T → S in AAF01345. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 28, 2002. Version 2.
Checksum: FFB90438BA53A02B

FASTA2,778311,143
        10         20         30         40         50         60 
MTFDTRRHTT GQPGSTAPSS SSSTTSTTTT TTSPAQSAGS GSGIGTGTGT VANSSLPGGG 

        70         80         90        100        110        120 
SGSLDGNQDQ QPATDSQSSD DVAASLSANS VDSTITIVPP EKLISSFPTT KLRSLTQKIS 

       130        140        150        160        170        180 
NPRWVVPVLP EQELEVLLNA AIELTQAGVD HDCEPCVEFY RNGLSTSFAK ILTDEAVNSW 

       190        200        210        220        230        240 
KNNIHHCILV SCGKLLHLIA IHMQRDNPYL LDLLAIVFDP ENKFNTFNAG RQPECFAAPD 

       250        260        270        280        290        300 
YIWGQLDSNK MYARPPPEPK NARGWLVDLI NRFGQLGGFD NLLERFNIGL ELLKRNQNKC 

       310        320        330        340        350        360 
TGKNISVEGR VENGAQDNRL TLALIHSLLR PFGQCYELLM PATIAKYFMP TWNVVLDLLD 

       370        380        390        400        410        420 
SFTDEELKRE VKPEGRNDYI NGIVKSARLL ASRLTGQEEL IRDLEMFRLK MILRLLQVSS 

       430        440        450        460        470        480 
FNGKMNALNE INKVLSSVAY FSHRSQPLPH CMPEDEMDWL TADRMAQWIK SSDVLGVVLK 

       490        500        510        520        530        540 
DSLHQPQYVE KLEKIIRFLI KEQALTLDDL DAVWRAQAGK HEAIVKNVHD LLAKLAWDFT 

       550        560        570        580        590        600 
PEQLDHLFEA FQASMTTANK RQRERLLELI RRLAEDDKNG VMAQKVLKLF WTLAHSQEVP 

       610        620        630        640        650        660 
PEVLDQALGA HVKILDYSCS QERDAQKTIW LDKCVDELKS GDGWVLPALR LIRDICCLYD 

       670        680        690        700        710        720 
TTTNHAQRTQ TSTNRQQVIE RLQNDYSLVI LVTNSLTAYM EKVRQMVTDS PGLDATRILI 

       730        740        750        760        770        780 
DGRFPHHVQI AERLEFLKFL LKDGQLWLCA DQAKQIWHCL AVNAVFPADR EECFRWFGKL 

       790        800        810        820        830        840 
MGEEPDLDPG INKDFFENNI LQLDPHLLTE SGIKCFERFF KAVNSKEDKL KAIHRGYMLD 

       850        860        870        880        890        900 
NEDLIGKDYL WRVITTGGEE IASKAIDLLK EVSTALGPRL QENIAEFHEM FIGECCSRLR 

       910        920        930        940        950        960 
THYGNIVILG KTQLQEELDA PDQSDNTNDE SKDSKMRFIE AEKMCRILKV LQEYVKECDR 

       970        980        990       1000       1010       1020 
SFSGDRVHLP LSRVTRGKNT ILYIRFQNPG RSIDDMEIVT HSNETMAAFK RNLLKRIKGT 

      1030       1040       1050       1060       1070       1080 
STANIKVDLF YANDEMIGVS DEINPLYQYT IRDKMNLTAK LTPVGTGLAS SPDSSSDSST 

      1090       1100       1110       1120       1130       1140 
GSPPRPCPDM QRVESESTLP GVIISQNYQY TEFFLKLYQL GSDLEHGRLR DSAKVLLHLL 

      1150       1160       1170       1180       1190       1200 
PCDRQTIRQL KIMCKVPKAA VTVAVTGDKI AKDEEEKLYP TEQAGIEDEE EHCTPEQMFL 

      1210       1220       1230       1240       1250       1260 
HPTPAQVLYN LSVLHGLLIP ALDPLGESAL LVQSAWMHSG CAHFVLELLT KNNFLPSADM 

      1270       1280       1290       1300       1310       1320 
HTKRASFQCV LRLAKLFLYI VGSVLSRVGD EPMICDLDNG SRSQVDILKQ NFSTMPSSSQ 

      1330       1340       1350       1360       1370       1380 
GTLRAISAKL AVILAREMLS ASPEGDRCRT LFSSTLQWSC PDISTIKAVV QLAWASSCGN 

      1390       1400       1410       1420       1430       1440 
LQALGNSSGD FEDEVIVPDG QDFSMCKEAL EVLTISFILN PSANEALTSD PNWPKFITSI 

      1450       1460       1470       1480       1490       1500 
VLKNPLRHVR QVASEQLFLA STYCAGDRRP FVYMVNLLVG ALKTLVPQYE STCAEFFSVL 

      1510       1520       1530       1540       1550       1560 
CRTLSYGCIY NWPLQISEGL LGDEIKWLQR IRENVHATGD TQVHEELLEG HLCLAKELMF 

      1570       1580       1590       1600       1610       1620 
FLGADSKAQL NELIHELIDD FLFTASREFL HLRRHGSLRQ DTVPPPVCRS PHTIAAACDL 

      1630       1640       1650       1660       1670       1680 
LIALCQLCVP NMKLLTNTLI DFVCTDTDPL REWDYLPPVG ARPTKGFCGL KNAGATCYMN 

      1690       1700       1710       1720       1730       1740 
SVLQQLYMVP AVRVGILRAH GAATTDGEDF SGDSDLTGGG LGSALFSGPA SALVSLPSSS 

      1750       1760       1770       1780       1790       1800 
STIEDGLHDV RKNYHVVILK HVQAIFAHLG HSALQYYVPR GLWTHFKLLG EPVNLREQQD 

      1810       1820       1830       1840       1850       1860 
AVEFFMSLLE SLDEGLKALG QPQLMNATLG GSFSDQKICQ ECPHRYSKEE PFSVFSVDIR 

      1870       1880       1890       1900       1910       1920 
NHSSLTESLE QYVKGELLEG ADAYHCDKCD KKVVTVKRVC VKKLPPVLAI QLKRFEYDYE 

      1930       1940       1950       1960       1970       1980 
RVCAIKFNDY FEFPRILDME PYTVSGLAKL EGEVVEVGDN CQTNVETTKY ELTGIVVHSG 

      1990       2000       2010       2020       2030       2040 
QASGGHYFSY ILSKNPANGK CQWYKFDDGE VTECKMHEDE EMKAECFGGE YMGETYDNNL 

      2050       2060       2070       2080       2090       2100 
KRMQYRRQKR WWNAYMLFYT RCDQTPVQYE PSVEQLSLAE SRNMVLPLPK PIERSVRHQN 

      2110       2120       2130       2140       2150       2160 
IRFLHSRSIF SVEFFNFIKK LVSCNLLSAR SNKITPAAEE LSLLGVQLAS QFLFHTGFRT 

      2170       2180       2190       2200       2210       2220 
KKSLRGPVME WYDALSHHIR SSALVRKWFA NHALLSPPSR LGEYILMAPS PDVRTVFVKL 

      2230       2240       2250       2260       2270       2280 
VVFFCHFAIN DEPLTGYDGA NLCEQVLISV LRLLKSEAAD YGKHLPHYFS LFSMYVGLGT 

      2290       2300       2310       2320       2330       2340 
REKQQLLRLN VPLQFIQVAL DDGPGPAIKY QYPEFSKLHQ VVSHLIRCSD VSEKCQSSNQ 

      2350       2360       2370       2380       2390       2400 
NARPLSNPFK DPNVAHEELT PLSTECMDLL FNRTGYIKKV IEDTNVGDEG LKLLQYCSWE 

      2410       2420       2430       2440       2450       2460 
NPHFSRAVLT ELLWQCGFAY CHDMRHHTDL LLNILLIDDS WQHHRIHNAL NGVAEEREGL 

      2470       2480       2490       2500       2510       2520 
LETIQRAKTH YQKRAYQIIK CLTQLFHKSP IALQMLHTNS NITRHWSIAV EWLQGELDRQ 

      2530       2540       2550       2560       2570       2580 
RGIGCQYNSY SWSPPAQSND NTNGYMLERS QSAKNTWSMA FELCPDEVSE KTDENNEPNL 

      2590       2600       2610       2620       2630       2640 
ETNMDENKSE PVAQPGGVLE GSTGGTEQLP ENKTPTTSSP STAAWPARGD SNAIPRLSRQ 

      2650       2660       2670       2680       2690       2700 
LFGAYTSTGS GSTSGGSAPT SALTTTAGSG ANSETESSAQ ETTGETTING LTNSLDQMEI 

      2710       2720       2730       2740       2750       2760 
TAKKKCRRVI IKKLVESKDE EDATTATTAA TTEVTTSPAT AIATAATLEP AGMSELTTMV 

      2770 
EKNLIISQEN PQAKSSLQ 

« Hide

Isoform 3 [UniParc].

Checksum: 0D6319FFCC88EA5C
Show »

FASTA2,711304,080

References

« Hide 'large scale' references
[1]"The fat facets gene is required for Drosophila eye and embryo development."
Fischer-Vize J.A., Rubin G.M., Lehmann R.
Development 116:985-1000(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY.
Tissue: Eye imaginal disk.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila complementary DNA resource."
Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M., Harvey D.A.
Science 287:2222-2224(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1089-2778 (ISOFORM 1).
Strain: Berkeley.
Tissue: Embryo.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-924, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04959 mRNA. Translation: AAF01345.1. Frameshift.
L04958 mRNA. Translation: AAF01346.1.
L04960 Genomic DNA. Translation: AAF01347.1.
L04960 Genomic DNA. Translation: AAF01348.1.
AE014297 Genomic DNA. Translation: AAF57198.1.
AE014297 Genomic DNA. Translation: AAN14291.1.
AF145677 mRNA. Translation: AAD38652.1.
PIRB49132.
RefSeqNP_524612.2. NM_079873.4.
NP_733455.1. NM_170576.2.
UniGeneDm.3133.

3D structure databases

ProteinModelPortalP55824.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid68590. 15 interactions.

Protein family/group databases

MEROPSC19.007.

Proteomic databases

PaxDbP55824.
PRIDEP55824.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0085843; FBpp0085202; FBgn0005632. [P55824-1]
GeneID43749.
KEGGdme:Dmel_CG1945.

Organism-specific databases

FlyBaseFBgn0005632. faf.

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00740000115055.
InParanoidP55824.
KOK11840.
OMAMAQEQFF.
OrthoDBEOG722J7K.
PhylomeDBP55824.

Gene expression databases

BgeeP55824.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 6 hits.
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSfaf. drosophila.
GenomeRNAi43749.
NextBio835588.
PROP55824.

Entry information

Entry nameFAF_DROME
AccessionPrimary (citable) accession number: P55824
Secondary accession number(s): Q9V9T6, Q9Y0Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2002
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase