Reviewed, UniProtKB/Swiss-Prot P55807 (NRT2_CHICK)

Names and origin

Protein names	Recommended name:     NAD(P)(+)--arginine ADP-ribosyltransferase 2     EC=2.4.2.31 Alternative name(s):     Mono(ADP-ribosyl)transferase 2       Short name=AT2
Organism	Gallus gallus (Chicken)
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Secreted › extracellular space. Note: The mature enzyme is probably secreted from granulocytes into the extracellular space.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 31	11	By similarity		PRO_0000019338
	Chain	32 – 266	235	NAD(P)(+)--arginine ADP-ribosyltransferase 2		PRO_0000019339
	Propeptide	267 – 312	46	Potential		PRO_0000019340
Sites
	Active site	231	1	By similarity
	Binding site	108	1	NAD By similarity
	Binding site	164	1	NAD By similarity
	Binding site	183	1	NAD By similarity
	Binding site	217	1	NAD By similarity
Amino acid modifications
	Disulfide bond	51 ↔ 260		By similarity
	Disulfide bond	159 ↔ 208		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55807-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9E45A9B7A6BC4057	FASTA	312	34,966
	10 20 30 40 50 60 MELLALRWVL LAGTLLSTSA ASSALQEGDL GSITVIMDMA PNSFDDQYVG CAHVMWANLQ 70 80 90 100 110 120 KLKCTEFARN YAYAVGWRKA AAEWQKRWGY LAHPMQLRPE QAIALLAYSA ASNLYQQFNA 130 140 150 160 170 180 ATRQGGCSHQ YYVHFYHFKT LHFLLTQALF ALRASQPRCY YVYRGVRGIR FMTQRGKSVR 190 200 210 220 230 240 FGQFTSTSLR KDVAVNFGQD TFFVVKTCYG VPIKQFSFYP SEDEVLIPPF EVFEVTNFCT 250 260 270 280 290 300 GNGRIQIYLR SKGKMSRHNC ELLKPRGGQW GRGHQEVGLG LSPGLALPVL PCSNCSCWGS 310 GHRAGDPIPA AV

References

[1]

"Cloning and expression of cDNA for arginine-specific ADP-ribosyltransferase from chicken bone marrow cells." Tsuchiya M., Hara N., Yamada K., Osago H., Shimoyama M. J. Biol. Chem. 269:27451-27457(1994) [PubMed: 7961658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Bone marrow.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D31865 mRNA. Translation: BAA06665.1.
IPI	IPI00592681.
PIR	B55461.
RefSeq	NP_990186.1.
UniGene	Gga.390
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALT00000020581; ENSGALP00000020552; ENSGALG00000012605; Gallus gallus. [Genome view]
GeneID	395661.
KEGG	gga:395661.
Organism-specific databases
CTD	395661.
Phylogenomic databases
HOVERGEN	P55807.
Enzyme and pathway databases
BRENDA	2.4.2.31. 4.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRT2_CHICK

Accession

Primary (citable) accession number: P55807

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 1, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)