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P55799 (PRP2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2

EC=3.1.3.16
Gene names
Name:pphB
Synonyms:prpB, ygbH
Ordered Locus Names:b2734, JW2704
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Sequence similarities

Belongs to the PPP phosphatase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Serine/threonine-protein phosphatase 2
PRO_0000058910

Sites

Active site781Proton donor By similarity
Metal binding221Iron By similarity
Metal binding241Iron By similarity
Metal binding511Iron By similarity
Metal binding511Manganese By similarity
Metal binding771Manganese By similarity
Metal binding1871Manganese By similarity

Experimental info

Mutagenesis781H → N in prpB17; loss of function. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P55799 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 965B7935E01FAE33

FASTA21825,098
        10         20         30         40         50         60 
MPSTRYQKIN AHHYRHIWVV GDIHGEYQLL QSRLHQLSFF PKIDLLISVG DNIDRGPESL 

        70         80         90        100        110        120 
DVLRLLNQPW FTSVKGNHEA MALEAFETGD GNMWLASGGD WFFDLNDSEQ QEAIDLLLKF 

       130        140        150        160        170        180 
HHLPHIIEIT NDNIKYAIAH ADYPGSEYLF GKEIAESELL WPVDRVQKSL NGELQQINGA 

       190        200        210 
DYFIFGHMMF DNIQTFANQI YIDTGSPNSG RLSFYKIK 

« Hide

References

« Hide 'large scale' references
[1]"Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
Missiakas D., Raina S.
EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-78.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51682 Genomic DNA. Translation: AAB53933.1.
U29579 Genomic DNA. Translation: AAA69244.1.
U00096 Genomic DNA. Translation: AAC75776.1.
AP009048 Genomic DNA. Translation: BAE76811.1.
PIRB65054.
RefSeqNP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

3D structure databases

ProteinModelPortalP55799.
SMRP55799. Positions 6-218.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP55799. 5 interactions.
STRING511145.b2734.

Protein family/group databases

PptaseDBP3D0407132.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75776; AAC75776; b2734.
BAE76811; BAE76811; BAE76811.
GeneID12932426.
947196.
KEGGecj:Y75_p2672.
eco:b2734.
PATRIC32120870. VBIEscCol129921_2827.

Organism-specific databases

EchoBASEEB2905.
EcoGeneEG13102. pphB.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000251869.
KOK07314.
OMAEINGADC.
OrthoDBEOG6KDKSC.
PhylomeDBP55799.
ProtClustDBPRK09968.

Enzyme and pathway databases

BioCycEcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Gene expression databases

GenevestigatorP55799.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP55799.

Entry information

Entry namePRP2_ECOLI
AccessionPrimary (citable) accession number: P55799
Secondary accession number(s): Q2MA95
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene