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P55799

- PRP2_ECOLI

UniProt

P55799 - PRP2_ECOLI

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Protein
Serine/threonine-protein phosphatase 2
Gene
pphB, prpB, ygbH, b2734, JW2704
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Manganese 1 By similarity
Metal bindingi24 – 241Manganese 1 By similarity
Metal bindingi51 – 511Manganese 1 By similarity
Metal bindingi51 – 511Manganese 2 By similarity
Metal bindingi77 – 771Manganese 2 By similarity
Active sitei78 – 781Proton donor By similarity
Metal bindingi187 – 1871Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: EcoCyc

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Protein family/group databases

PptaseDBiP3D0407132.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2 (EC:3.1.3.16)
Gene namesi
Name:pphB
Synonyms:prpB, ygbH
Ordered Locus Names:b2734, JW2704
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13102. pphB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781H → N in prpB17; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Serine/threonine-protein phosphatase 2
PRO_0000058910Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP55799.

Interactioni

Protein-protein interaction databases

IntActiP55799. 5 interactions.
STRINGi511145.b2734.

Structurei

3D structure databases

ProteinModelPortaliP55799.
SMRiP55799. Positions 6-218.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
KOiK07314.
OMAiGDWFFEL.
OrthoDBiEOG6KDKSC.
PhylomeDBiP55799.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55799-1 [UniParc]FASTAAdd to Basket

« Hide

MPSTRYQKIN AHHYRHIWVV GDIHGEYQLL QSRLHQLSFF PKIDLLISVG    50
DNIDRGPESL DVLRLLNQPW FTSVKGNHEA MALEAFETGD GNMWLASGGD 100
WFFDLNDSEQ QEAIDLLLKF HHLPHIIEIT NDNIKYAIAH ADYPGSEYLF 150
GKEIAESELL WPVDRVQKSL NGELQQINGA DYFIFGHMMF DNIQTFANQI 200
YIDTGSPNSG RLSFYKIK 218
Length:218
Mass (Da):25,098
Last modified:November 1, 1997 - v1
Checksum:i965B7935E01FAE33
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51682 Genomic DNA. Translation: AAB53933.1.
U29579 Genomic DNA. Translation: AAA69244.1.
U00096 Genomic DNA. Translation: AAC75776.1.
AP009048 Genomic DNA. Translation: BAE76811.1.
PIRiB65054.
RefSeqiNP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75776; AAC75776; b2734.
BAE76811; BAE76811; BAE76811.
GeneIDi12932426.
947196.
KEGGiecj:Y75_p2672.
eco:b2734.
PATRICi32120870. VBIEscCol129921_2827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51682 Genomic DNA. Translation: AAB53933.1 .
U29579 Genomic DNA. Translation: AAA69244.1 .
U00096 Genomic DNA. Translation: AAC75776.1 .
AP009048 Genomic DNA. Translation: BAE76811.1 .
PIRi B65054.
RefSeqi NP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

3D structure databases

ProteinModelPortali P55799.
SMRi P55799. Positions 6-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P55799. 5 interactions.
STRINGi 511145.b2734.

Protein family/group databases

PptaseDBi P3D0407132.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75776 ; AAC75776 ; b2734 .
BAE76811 ; BAE76811 ; BAE76811 .
GeneIDi 12932426.
947196.
KEGGi ecj:Y75_p2672.
eco:b2734.
PATRICi 32120870. VBIEscCol129921_2827.

Organism-specific databases

EchoBASEi EB2905.
EcoGenei EG13102. pphB.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000251869.
KOi K07314.
OMAi GDWFFEL.
OrthoDBi EOG6KDKSC.
PhylomeDBi P55799.

Enzyme and pathway databases

BioCyci EcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Miscellaneous databases

PROi P55799.

Gene expression databases

Genevestigatori P55799.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
    Missiakas D., Raina S.
    EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-78.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRP2_ECOLI
AccessioniPrimary (citable) accession number: P55799
Secondary accession number(s): Q2MA95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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