P55799 (PRP2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2
EC=3.1.3.16

Gene names

Name:	pphB
Synonyms:	prpB, ygbH
Ordered Locus Names:	b2734, JW2704

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Serine/threonine-protein phosphatase 2		PRO_0000058910
Sites
	Active site	78	1	Proton donor By similarity
	Metal binding	22	1	Iron By similarity
	Metal binding	24	1	Iron By similarity
	Metal binding	51	1	Iron By similarity
	Metal binding	51	1	Manganese By similarity
	Metal binding	77	1	Manganese By similarity
	Metal binding	187	1	Manganese By similarity
Experimental info
	Mutagenesis	78	1	H → N in prpB17; loss of function. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P55799 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 965B7935E01FAE33	FASTA	218	25,098
	10 20 30 40 50 60 MPSTRYQKIN AHHYRHIWVV GDIHGEYQLL QSRLHQLSFF PKIDLLISVG DNIDRGPESL 70 80 90 100 110 120 DVLRLLNQPW FTSVKGNHEA MALEAFETGD GNMWLASGGD WFFDLNDSEQ QEAIDLLLKF 130 140 150 160 170 180 HHLPHIIEIT NDNIKYAIAH ADYPGSEYLF GKEIAESELL WPVDRVQKSL NGELQQINGA 190 200 210 DYFIFGHMMF DNIQTFANQI YIDTGSPNSG RLSFYKIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB." Missiakas D., Raina S. EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-78. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51682 Genomic DNA. Translation: AAB53933.1. U29579 Genomic DNA. Translation: AAA69244.1. U00096 Genomic DNA. Translation: AAC75776.1. AP009048 Genomic DNA. Translation: BAE76811.1.
PIR	B65054.
RefSeq	NP_417214.1. NC_000913.2. YP_490943.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P55799.
SMR	P55799. Positions 6-218.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55799. 5 interactions.
STRING	511145.b2734.
Protein family/group databases
PptaseDB	P3D0407132.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75776; AAC75776; b2734. BAE76811; BAE76811; BAE76811.
GeneID	12932426. 947196.
KEGG	ecj:Y75_p2672. eco:b2734.
PATRIC	32120870. VBIEscCol129921_2827.
Organism-specific databases
EchoBASE	EB2905.
EcoGene	EG13102. pphB.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000251869.
KO	K07314.
OMA	NTSWFIS.
ProtClustDB	PRK09968.
Enzyme and pathway databases
BioCyc	EcoCyc:G7415-MONOMER. ECOL316407:JW2704-MONOMER. MetaCyc:G7415-MONOMER.
Gene expression databases
Genevestigator	P55799.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRP2_ECOLI

Accession

Primary (citable) accession number: P55799
Secondary accession number(s): Q2MA95

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program