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Protein

Serine/threonine-protein phosphatase 2

Gene

pphB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Manganese 1By similarity
Metal bindingi24 – 241Manganese 1By similarity
Metal bindingi51 – 511Manganese 1By similarity
Metal bindingi51 – 511Manganese 2By similarity
Metal bindingi77 – 771Manganese 2By similarity
Active sitei78 – 781Proton donorBy similarity
Metal bindingi187 – 1871Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: EcoCyc

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Protein family/group databases

PptaseDBiP3D0407132.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2 (EC:3.1.3.16)
Gene namesi
Name:pphB
Synonyms:prpB, ygbH
Ordered Locus Names:b2734, JW2704
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13102. pphB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781H → N in prpB17; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Serine/threonine-protein phosphatase 2PRO_0000058910Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP55799.

Interactioni

Protein-protein interaction databases

IntActiP55799. 5 interactions.
STRINGi511145.b2734.

Structurei

3D structure databases

ProteinModelPortaliP55799.
SMRiP55799. Positions 6-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
InParanoidiP55799.
KOiK07314.
OMAiNTSWFIS.
OrthoDBiEOG6KDKSC.
PhylomeDBiP55799.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSTRYQKIN AHHYRHIWVV GDIHGEYQLL QSRLHQLSFF PKIDLLISVG
60 70 80 90 100
DNIDRGPESL DVLRLLNQPW FTSVKGNHEA MALEAFETGD GNMWLASGGD
110 120 130 140 150
WFFDLNDSEQ QEAIDLLLKF HHLPHIIEIT NDNIKYAIAH ADYPGSEYLF
160 170 180 190 200
GKEIAESELL WPVDRVQKSL NGELQQINGA DYFIFGHMMF DNIQTFANQI
210
YIDTGSPNSG RLSFYKIK
Length:218
Mass (Da):25,098
Last modified:November 1, 1997 - v1
Checksum:i965B7935E01FAE33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51682 Genomic DNA. Translation: AAB53933.1.
U29579 Genomic DNA. Translation: AAA69244.1.
U00096 Genomic DNA. Translation: AAC75776.1.
AP009048 Genomic DNA. Translation: BAE76811.1.
PIRiB65054.
RefSeqiNP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75776; AAC75776; b2734.
BAE76811; BAE76811; BAE76811.
GeneIDi12932426.
947196.
KEGGiecj:Y75_p2672.
eco:b2734.
PATRICi32120870. VBIEscCol129921_2827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51682 Genomic DNA. Translation: AAB53933.1.
U29579 Genomic DNA. Translation: AAA69244.1.
U00096 Genomic DNA. Translation: AAC75776.1.
AP009048 Genomic DNA. Translation: BAE76811.1.
PIRiB65054.
RefSeqiNP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP55799.
SMRiP55799. Positions 6-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP55799. 5 interactions.
STRINGi511145.b2734.

Protein family/group databases

PptaseDBiP3D0407132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75776; AAC75776; b2734.
BAE76811; BAE76811; BAE76811.
GeneIDi12932426.
947196.
KEGGiecj:Y75_p2672.
eco:b2734.
PATRICi32120870. VBIEscCol129921_2827.

Organism-specific databases

EchoBASEiEB2905.
EcoGeneiEG13102. pphB.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
InParanoidiP55799.
KOiK07314.
OMAiNTSWFIS.
OrthoDBiEOG6KDKSC.
PhylomeDBiP55799.

Enzyme and pathway databases

BioCyciEcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Miscellaneous databases

PROiP55799.

Gene expression databases

GenevestigatoriP55799.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
    Missiakas D., Raina S.
    EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-78.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRP2_ECOLI
AccessioniPrimary (citable) accession number: P55799
Secondary accession number(s): Q2MA95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.