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P55799

- PRP2_ECOLI

UniProt

P55799 - PRP2_ECOLI

Protein

Serine/threonine-protein phosphatase 2

Gene

pphB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi22 – 221Manganese 1By similarity
    Metal bindingi24 – 241Manganese 1By similarity
    Metal bindingi51 – 511Manganese 1By similarity
    Metal bindingi51 – 511Manganese 2By similarity
    Metal bindingi77 – 771Manganese 2By similarity
    Active sitei78 – 781Proton donorBy similarity
    Metal bindingi187 – 1871Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: EcoCyc

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7415-MONOMER.
    ECOL316407:JW2704-MONOMER.
    MetaCyc:G7415-MONOMER.

    Protein family/group databases

    PptaseDBiP3D0407132.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2 (EC:3.1.3.16)
    Gene namesi
    Name:pphB
    Synonyms:prpB, ygbH
    Ordered Locus Names:b2734, JW2704
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13102. pphB.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi78 – 781H → N in prpB17; loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 218218Serine/threonine-protein phosphatase 2PRO_0000058910Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP55799.

    Interactioni

    Protein-protein interaction databases

    IntActiP55799. 5 interactions.
    STRINGi511145.b2734.

    Structurei

    3D structure databases

    ProteinModelPortaliP55799.
    SMRiP55799. Positions 6-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000251869.
    KOiK07314.
    OMAiGDWFFEL.
    OrthoDBiEOG6KDKSC.
    PhylomeDBiP55799.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55799-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSTRYQKIN AHHYRHIWVV GDIHGEYQLL QSRLHQLSFF PKIDLLISVG    50
    DNIDRGPESL DVLRLLNQPW FTSVKGNHEA MALEAFETGD GNMWLASGGD 100
    WFFDLNDSEQ QEAIDLLLKF HHLPHIIEIT NDNIKYAIAH ADYPGSEYLF 150
    GKEIAESELL WPVDRVQKSL NGELQQINGA DYFIFGHMMF DNIQTFANQI 200
    YIDTGSPNSG RLSFYKIK 218
    Length:218
    Mass (Da):25,098
    Last modified:November 1, 1997 - v1
    Checksum:i965B7935E01FAE33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51682 Genomic DNA. Translation: AAB53933.1.
    U29579 Genomic DNA. Translation: AAA69244.1.
    U00096 Genomic DNA. Translation: AAC75776.1.
    AP009048 Genomic DNA. Translation: BAE76811.1.
    PIRiB65054.
    RefSeqiNP_417214.1. NC_000913.3.
    YP_490943.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75776; AAC75776; b2734.
    BAE76811; BAE76811; BAE76811.
    GeneIDi12932426.
    947196.
    KEGGiecj:Y75_p2672.
    eco:b2734.
    PATRICi32120870. VBIEscCol129921_2827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51682 Genomic DNA. Translation: AAB53933.1 .
    U29579 Genomic DNA. Translation: AAA69244.1 .
    U00096 Genomic DNA. Translation: AAC75776.1 .
    AP009048 Genomic DNA. Translation: BAE76811.1 .
    PIRi B65054.
    RefSeqi NP_417214.1. NC_000913.3.
    YP_490943.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P55799.
    SMRi P55799. Positions 6-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P55799. 5 interactions.
    STRINGi 511145.b2734.

    Protein family/group databases

    PptaseDBi P3D0407132.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75776 ; AAC75776 ; b2734 .
    BAE76811 ; BAE76811 ; BAE76811 .
    GeneIDi 12932426.
    947196.
    KEGGi ecj:Y75_p2672.
    eco:b2734.
    PATRICi 32120870. VBIEscCol129921_2827.

    Organism-specific databases

    EchoBASEi EB2905.
    EcoGenei EG13102. pphB.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000251869.
    KOi K07314.
    OMAi GDWFFEL.
    OrthoDBi EOG6KDKSC.
    PhylomeDBi P55799.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7415-MONOMER.
    ECOL316407:JW2704-MONOMER.
    MetaCyc:G7415-MONOMER.

    Miscellaneous databases

    PROi P55799.

    Gene expression databases

    Genevestigatori P55799.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
      Missiakas D., Raina S.
      EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-78.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPRP2_ECOLI
    AccessioniPrimary (citable) accession number: P55799
    Secondary accession number(s): Q2MA95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3