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P55799

- PRP2_ECOLI

UniProt

P55799 - PRP2_ECOLI

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Protein

Serine/threonine-protein phosphatase 2

Gene

pphB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Manganese 1By similarity
Metal bindingi24 – 241Manganese 1By similarity
Metal bindingi51 – 511Manganese 1By similarity
Metal bindingi51 – 511Manganese 2By similarity
Metal bindingi77 – 771Manganese 2By similarity
Active sitei78 – 781Proton donorBy similarity
Metal bindingi187 – 1871Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: EcoCyc

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Protein family/group databases

PptaseDBiP3D0407132.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2 (EC:3.1.3.16)
Gene namesi
Name:pphB
Synonyms:prpB, ygbH
Ordered Locus Names:b2734, JW2704
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13102. pphB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781H → N in prpB17; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Serine/threonine-protein phosphatase 2PRO_0000058910Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP55799.

Interactioni

Protein-protein interaction databases

IntActiP55799. 5 interactions.
STRINGi511145.b2734.

Structurei

3D structure databases

ProteinModelPortaliP55799.
SMRiP55799. Positions 6-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
InParanoidiP55799.
KOiK07314.
OMAiGDWFFEL.
OrthoDBiEOG6KDKSC.
PhylomeDBiP55799.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSTRYQKIN AHHYRHIWVV GDIHGEYQLL QSRLHQLSFF PKIDLLISVG
60 70 80 90 100
DNIDRGPESL DVLRLLNQPW FTSVKGNHEA MALEAFETGD GNMWLASGGD
110 120 130 140 150
WFFDLNDSEQ QEAIDLLLKF HHLPHIIEIT NDNIKYAIAH ADYPGSEYLF
160 170 180 190 200
GKEIAESELL WPVDRVQKSL NGELQQINGA DYFIFGHMMF DNIQTFANQI
210
YIDTGSPNSG RLSFYKIK
Length:218
Mass (Da):25,098
Last modified:November 1, 1997 - v1
Checksum:i965B7935E01FAE33
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51682 Genomic DNA. Translation: AAB53933.1.
U29579 Genomic DNA. Translation: AAA69244.1.
U00096 Genomic DNA. Translation: AAC75776.1.
AP009048 Genomic DNA. Translation: BAE76811.1.
PIRiB65054.
RefSeqiNP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75776; AAC75776; b2734.
BAE76811; BAE76811; BAE76811.
GeneIDi12932426.
947196.
KEGGiecj:Y75_p2672.
eco:b2734.
PATRICi32120870. VBIEscCol129921_2827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51682 Genomic DNA. Translation: AAB53933.1 .
U29579 Genomic DNA. Translation: AAA69244.1 .
U00096 Genomic DNA. Translation: AAC75776.1 .
AP009048 Genomic DNA. Translation: BAE76811.1 .
PIRi B65054.
RefSeqi NP_417214.1. NC_000913.3.
YP_490943.1. NC_007779.1.

3D structure databases

ProteinModelPortali P55799.
SMRi P55799. Positions 6-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P55799. 5 interactions.
STRINGi 511145.b2734.

Protein family/group databases

PptaseDBi P3D0407132.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75776 ; AAC75776 ; b2734 .
BAE76811 ; BAE76811 ; BAE76811 .
GeneIDi 12932426.
947196.
KEGGi ecj:Y75_p2672.
eco:b2734.
PATRICi 32120870. VBIEscCol129921_2827.

Organism-specific databases

EchoBASEi EB2905.
EcoGenei EG13102. pphB.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000251869.
InParanoidi P55799.
KOi K07314.
OMAi GDWFFEL.
OrthoDBi EOG6KDKSC.
PhylomeDBi P55799.

Enzyme and pathway databases

BioCyci EcoCyc:G7415-MONOMER.
ECOL316407:JW2704-MONOMER.
MetaCyc:G7415-MONOMER.

Miscellaneous databases

PROi P55799.

Gene expression databases

Genevestigatori P55799.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
    Missiakas D., Raina S.
    EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-78.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRP2_ECOLI
AccessioniPrimary (citable) accession number: P55799
Secondary accession number(s): Q2MA95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3