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P55798

- PRP1_ECOLI

UniProt

P55798 - PRP1_ECOLI

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Protein

Serine/threonine-protein phosphatase 1

Gene

pphA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Manganese 1By similarity
Metal bindingi26 – 261Manganese 1By similarity
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi53 – 531Manganese 2By similarity
Metal bindingi79 – 791Manganese 2By similarity
Active sitei80 – 801Proton donorBy similarity
Metal bindingi187 – 1871Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein tyrosine/serine/threonine phosphatase activity Source: EcoCyc

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. response to temperature stimulus Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7011-MONOMER.
ECOL316407:JW1827-MONOMER.
MetaCyc:G7011-MONOMER.

Protein family/group databases

PptaseDBiP3D0407131.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 1 (EC:3.1.3.16)
Gene namesi
Name:pphA
Synonyms:prpA, yebX
Ordered Locus Names:b1838, JW1827
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14026. pphA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241D → V: Loss of function. 1 Publication
Mutagenesisi26 – 261H → L: Loss of function. 1 Publication
Mutagenesisi26 – 261H → N: Loss of function. 1 Publication
Mutagenesisi107 – 1071L → Q: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Serine/threonine-protein phosphatase 1PRO_0000058908Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP55798.

Interactioni

Protein-protein interaction databases

IntActiP55798. 1 interaction.
STRINGi511145.b1838.

Structurei

3D structure databases

ProteinModelPortaliP55798.
SMRiP55798. Positions 8-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
InParanoidiP55798.
KOiK07313.
OMAiHDEFVIN.
OrthoDBiEOG6KDKSC.
PhylomeDBiP55798.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55798-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQPAPVYQR IAGHQWRHIW LSGDIHGCLE QLRRKLWHCR FDPWRDLLIS
60 70 80 90 100
VGDVIDRGPQ SLRCLQLLEQ HWVCAVRGNH EQMAMDALAS QQMSLWLMNG
110 120 130 140 150
GDWFIALADN QQKQAKTALE KCQHLPFILE VHSRTGKHVI AHADYPDDVY
160 170 180 190 200
EWQKDVDLHQ VLWSRSRLGE RQKGQGITGA DHFWFGHTPL RHRVDIGNLH
210
YIDTGAVFGG ELTLVQLQ
Length:218
Mass (Da):25,274
Last modified:November 1, 1997 - v1
Checksum:i9E6C452168004521
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51991 Genomic DNA. Translation: AAB53934.1.
U00096 Genomic DNA. Translation: AAC74908.2.
AP009048 Genomic DNA. Translation: BAA15649.2.
PIRiF64945.
RefSeqiNP_416352.4. NC_000913.3.
YP_490100.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74908; AAC74908; b1838.
BAA15649; BAA15649; BAA15649.
GeneIDi12932405.
946356.
KEGGiecj:Y75_p1814.
eco:b1838.
PATRICi32118997. VBIEscCol129921_1916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51991 Genomic DNA. Translation: AAB53934.1 .
U00096 Genomic DNA. Translation: AAC74908.2 .
AP009048 Genomic DNA. Translation: BAA15649.2 .
PIRi F64945.
RefSeqi NP_416352.4. NC_000913.3.
YP_490100.1. NC_007779.1.

3D structure databases

ProteinModelPortali P55798.
SMRi P55798. Positions 8-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P55798. 1 interaction.
STRINGi 511145.b1838.

Protein family/group databases

PptaseDBi P3D0407131.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74908 ; AAC74908 ; b1838 .
BAA15649 ; BAA15649 ; BAA15649 .
GeneIDi 12932405.
946356.
KEGGi ecj:Y75_p1814.
eco:b1838.
PATRICi 32118997. VBIEscCol129921_1916.

Organism-specific databases

EchoBASEi EB3780.
EcoGenei EG14026. pphA.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000251869.
InParanoidi P55798.
KOi K07313.
OMAi HDEFVIN.
OrthoDBi EOG6KDKSC.
PhylomeDBi P55798.

Enzyme and pathway databases

BioCyci EcoCyc:G7011-MONOMER.
ECOL316407:JW1827-MONOMER.
MetaCyc:G7011-MONOMER.

Miscellaneous databases

PROi P55798.

Gene expression databases

Genevestigatori P55798.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
    Missiakas D., Raina S.
    EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ASP-24; HIS-26 AND LEU-107.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRP1_ECOLI
AccessioniPrimary (citable) accession number: P55798
Secondary accession number(s): P76276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3