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Protein

Serine/threonine-protein phosphatase 1

Gene

pphA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24Manganese 1By similarity1
Metal bindingi26Manganese 1By similarity1
Metal bindingi53Manganese 1By similarity1
Metal bindingi53Manganese 2By similarity1
Metal bindingi79Manganese 2By similarity1
Active sitei80Proton donorBy similarity1
Metal bindingi187Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein tyrosine/serine/threonine phosphatase activity Source: EcoCyc

GO - Biological processi

  • response to temperature stimulus Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7011-MONOMER.
ECOL316407:JW1827-MONOMER.
MetaCyc:G7011-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 1 (EC:3.1.3.16)
Gene namesi
Name:pphA
Synonyms:prpA, yebX
Ordered Locus Names:b1838, JW1827
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14026. pphA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24D → V: Loss of function. 1 Publication1
Mutagenesisi26H → L: Loss of function. 1 Publication1
Mutagenesisi26H → N: Loss of function. 1 Publication1
Mutagenesisi107L → Q: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589081 – 218Serine/threonine-protein phosphatase 1Add BLAST218

Proteomic databases

PaxDbiP55798.
PRIDEiP55798.

Interactioni

Protein-protein interaction databases

BioGridi4261399. 7 interactors.
IntActiP55798. 1 interactor.
STRINGi511145.b1838.

Structurei

3D structure databases

ProteinModelPortaliP55798.
SMRiP55798.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG4108R1E. Bacteria.
COG0639. LUCA.
HOGENOMiHOG000251869.
InParanoidiP55798.
KOiK07313.
OMAiMNGGDWY.
PhylomeDBiP55798.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQPAPVYQR IAGHQWRHIW LSGDIHGCLE QLRRKLWHCR FDPWRDLLIS
60 70 80 90 100
VGDVIDRGPQ SLRCLQLLEQ HWVCAVRGNH EQMAMDALAS QQMSLWLMNG
110 120 130 140 150
GDWFIALADN QQKQAKTALE KCQHLPFILE VHSRTGKHVI AHADYPDDVY
160 170 180 190 200
EWQKDVDLHQ VLWSRSRLGE RQKGQGITGA DHFWFGHTPL RHRVDIGNLH
210
YIDTGAVFGG ELTLVQLQ
Length:218
Mass (Da):25,274
Last modified:November 1, 1997 - v1
Checksum:i9E6C452168004521
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51991 Genomic DNA. Translation: AAB53934.1.
U00096 Genomic DNA. Translation: AAC74908.2.
AP009048 Genomic DNA. Translation: BAA15649.2.
PIRiF64945.
RefSeqiNP_416352.4. NC_000913.3.
WP_000812724.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74908; AAC74908; b1838.
BAA15649; BAA15649; BAA15649.
GeneIDi946356.
KEGGiecj:JW1827.
eco:b1838.
PATRICi32118997. VBIEscCol129921_1916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51991 Genomic DNA. Translation: AAB53934.1.
U00096 Genomic DNA. Translation: AAC74908.2.
AP009048 Genomic DNA. Translation: BAA15649.2.
PIRiF64945.
RefSeqiNP_416352.4. NC_000913.3.
WP_000812724.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP55798.
SMRiP55798.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261399. 7 interactors.
IntActiP55798. 1 interactor.
STRINGi511145.b1838.

Proteomic databases

PaxDbiP55798.
PRIDEiP55798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74908; AAC74908; b1838.
BAA15649; BAA15649; BAA15649.
GeneIDi946356.
KEGGiecj:JW1827.
eco:b1838.
PATRICi32118997. VBIEscCol129921_1916.

Organism-specific databases

EchoBASEiEB3780.
EcoGeneiEG14026. pphA.

Phylogenomic databases

eggNOGiENOG4108R1E. Bacteria.
COG0639. LUCA.
HOGENOMiHOG000251869.
InParanoidiP55798.
KOiK07313.
OMAiMNGGDWY.
PhylomeDBiP55798.

Enzyme and pathway databases

BioCyciEcoCyc:G7011-MONOMER.
ECOL316407:JW1827-MONOMER.
MetaCyc:G7011-MONOMER.

Miscellaneous databases

PROiP55798.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP1_ECOLI
AccessioniPrimary (citable) accession number: P55798
Secondary accession number(s): P76276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.