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P55798

- PRP1_ECOLI

UniProt

P55798 - PRP1_ECOLI

Protein

Serine/threonine-protein phosphatase 1

Gene

pphA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi24 – 241Manganese 1By similarity
    Metal bindingi26 – 261Manganese 1By similarity
    Metal bindingi53 – 531Manganese 1By similarity
    Metal bindingi53 – 531Manganese 2By similarity
    Metal bindingi79 – 791Manganese 2By similarity
    Active sitei80 – 801Proton donorBy similarity
    Metal bindingi187 – 1871Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein tyrosine/serine/threonine phosphatase activity Source: EcoCyc

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. response to temperature stimulus Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7011-MONOMER.
    ECOL316407:JW1827-MONOMER.
    MetaCyc:G7011-MONOMER.

    Protein family/group databases

    PptaseDBiP3D0407131.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 1 (EC:3.1.3.16)
    Gene namesi
    Name:pphA
    Synonyms:prpA, yebX
    Ordered Locus Names:b1838, JW1827
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14026. pphA.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi24 – 241D → V: Loss of function. 1 Publication
    Mutagenesisi26 – 261H → L: Loss of function. 1 Publication
    Mutagenesisi26 – 261H → N: Loss of function. 1 Publication
    Mutagenesisi107 – 1071L → Q: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 218218Serine/threonine-protein phosphatase 1PRO_0000058908Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP55798.

    Interactioni

    Protein-protein interaction databases

    IntActiP55798. 1 interaction.
    STRINGi511145.b1838.

    Structurei

    3D structure databases

    ProteinModelPortaliP55798.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000251869.
    KOiK07313.
    OMAiHDEFVIN.
    OrthoDBiEOG6KDKSC.
    PhylomeDBiP55798.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55798-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQPAPVYQR IAGHQWRHIW LSGDIHGCLE QLRRKLWHCR FDPWRDLLIS    50
    VGDVIDRGPQ SLRCLQLLEQ HWVCAVRGNH EQMAMDALAS QQMSLWLMNG 100
    GDWFIALADN QQKQAKTALE KCQHLPFILE VHSRTGKHVI AHADYPDDVY 150
    EWQKDVDLHQ VLWSRSRLGE RQKGQGITGA DHFWFGHTPL RHRVDIGNLH 200
    YIDTGAVFGG ELTLVQLQ 218
    Length:218
    Mass (Da):25,274
    Last modified:November 1, 1997 - v1
    Checksum:i9E6C452168004521
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51991 Genomic DNA. Translation: AAB53934.1.
    U00096 Genomic DNA. Translation: AAC74908.2.
    AP009048 Genomic DNA. Translation: BAA15649.2.
    PIRiF64945.
    RefSeqiNP_416352.4. NC_000913.3.
    YP_490100.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74908; AAC74908; b1838.
    BAA15649; BAA15649; BAA15649.
    GeneIDi12932405.
    946356.
    KEGGiecj:Y75_p1814.
    eco:b1838.
    PATRICi32118997. VBIEscCol129921_1916.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51991 Genomic DNA. Translation: AAB53934.1 .
    U00096 Genomic DNA. Translation: AAC74908.2 .
    AP009048 Genomic DNA. Translation: BAA15649.2 .
    PIRi F64945.
    RefSeqi NP_416352.4. NC_000913.3.
    YP_490100.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P55798.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P55798. 1 interaction.
    STRINGi 511145.b1838.

    Protein family/group databases

    PptaseDBi P3D0407131.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74908 ; AAC74908 ; b1838 .
    BAA15649 ; BAA15649 ; BAA15649 .
    GeneIDi 12932405.
    946356.
    KEGGi ecj:Y75_p1814.
    eco:b1838.
    PATRICi 32118997. VBIEscCol129921_1916.

    Organism-specific databases

    EchoBASEi EB3780.
    EcoGenei EG14026. pphA.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000251869.
    KOi K07313.
    OMAi HDEFVIN.
    OrthoDBi EOG6KDKSC.
    PhylomeDBi P55798.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7011-MONOMER.
    ECOL316407:JW1827-MONOMER.
    MetaCyc:G7011-MONOMER.

    Miscellaneous databases

    PROi P55798.

    Gene expression databases

    Genevestigatori P55798.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
      Missiakas D., Raina S.
      EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ASP-24; HIS-26 AND LEU-107.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPRP1_ECOLI
    AccessioniPrimary (citable) accession number: P55798
    Secondary accession number(s): P76276
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3