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P55798 (PRP1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 1
EC=3.1.3.16
Gene names
Name:pphA
Synonyms:prpA, yebX
Ordered Locus Names:b1838, JW1827
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr and Tyr-phosphorylated substrates.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
   LigandIron
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Serine/threonine-protein phosphatase 1
PRO_0000058908

Sites

Active site801Proton donor By similarity
Metal binding241Iron By similarity
Metal binding261Iron By similarity
Metal binding531Iron By similarity
Metal binding531Manganese By similarity
Metal binding791Manganese By similarity
Metal binding1871Manganese By similarity

Experimental info

Mutagenesis241D → V: Loss of function. Ref.1
Mutagenesis261H → L: Loss of function. Ref.1
Mutagenesis261H → N: Loss of function. Ref.1
Mutagenesis1071L → Q: Loss of function. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P55798 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 9E6C452168004521

FASTA21825,274
        10         20         30         40         50         60 
MKQPAPVYQR IAGHQWRHIW LSGDIHGCLE QLRRKLWHCR FDPWRDLLIS VGDVIDRGPQ 

        70         80         90        100        110        120 
SLRCLQLLEQ HWVCAVRGNH EQMAMDALAS QQMSLWLMNG GDWFIALADN QQKQAKTALE 

       130        140        150        160        170        180 
KCQHLPFILE VHSRTGKHVI AHADYPDDVY EWQKDVDLHQ VLWSRSRLGE RQKGQGITGA 

       190        200        210 
DHFWFGHTPL RHRVDIGNLH YIDTGAVFGG ELTLVQLQ

« Hide

References

« Hide 'large scale' references
[1]"Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB."
Missiakas D., Raina S.
EMBO J. 16:1670-1685(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ASP-24; HIS-26 AND LEU-107.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U51991 Genomic DNA. Translation: AAB53934.1.
U00096 Genomic DNA. Translation: AAC74908.2.
AP009048 Genomic DNA. Translation: BAA15649.2.
PIRF64945.
RefSeqNP_416352.4. NC_000913.2.
YP_490100.1. NC_007779.1.

3D structure databases

ProteinModelPortalP55798.
SMRP55798. Positions 8-218.
ModBaseSearch...

Protein-protein interaction databases

IntActP55798. 1 interaction.
STRING511145.b1838.

Protein family/group databases

PptaseDBP3D0407131.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74908; AAC74908; b1838.
BAA15649; BAA15649; BAA15649.
GeneID12932405.
946356.
KEGGecj:Y75_p1814.
eco:b1838.
PATRIC32118997. VBIEscCol129921_1916.

Organism-specific databases

EchoBASEEB3780.
EcoGeneEG14026. pphA.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000251869.
KOK07313.
OMAMMKQPAP.
ProtClustDBPRK11439.

Enzyme and pathway databases

BioCycEcoCyc:G7011-MONOMER.
ECOL316407:JW1827-MONOMER.
MetaCyc:G7011-MONOMER.

Gene expression databases

GenevestigatorP55798.

Family and domain databases

InterProIPR004843. Metallo_PEstase_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRP1_ECOLI
AccessionPrimary (citable) accession number: P55798
Secondary accession number(s): P76276
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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