Reviewed, UniProtKB/Swiss-Prot P55786 (PSA_HUMAN)

Names and origin

Protein names

Recommended name:
    Puromycin-sensitive aminopeptidase
      Short name=PSA
    EC=3.4.11.-

Gene names

Name:	NPEPPS
Synonyms:	PSA

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	919 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Aminopeptidase with broad substrate specificity to several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm Potential. Nucleus Potential.
Sequence similarities	Belongs to the peptidase M1 family.
Caution	It is uncertain whether Met-1 or Met-45 is the initiator.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Ref.1 Traceable author statement. Source: ProtInc nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Ref.5 Traceable author statement. Source: ProtInc metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF6	P56537	1	EBI-1046129,EBI-372243

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 919	919	Puromycin-sensitive aminopeptidase		PRO_0000095116
Regions
	Motif	726 – 730	5	Nuclear localization signal Potential
Sites
	Active site	353	1	By similarity
	Active site	404	1	Proton donor Potential
	Metal binding	352	1	Zinc; catalytic By similarity
	Metal binding	356	1	Zinc; catalytic By similarity
	Metal binding	375	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	48	1	N6-acetyllysine Ref.7
Experimental info
	Sequence conflict	184	1	A → P in CAA68964. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P55786-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 873C9BF75494A057	FASTA	919	103,276
	10 20 30 40 50 60 MWLAAAAPSL ARRLLFLGPP PPPLLLLVFS RSSRRRLHSL GLAAMPEKRP FERLPADVSP 70 80 90 100 110 120 INYSLCLKPD LLDFTFEGKL EAAAQVRQAT NQIVMNCADI DIITASYAPE GDEEIHATGF 130 140 150 160 170 180 NYQNEDEKVT LSFPSTLQTG TGTLKIDFVG ELNDKMKGFY RSKYTTPSGE VRYAAVTQFE 190 200 210 220 230 240 ATDARRAFPC WDEPAIKATF DISLVVPKDR VALSNMNVID RKPYPDDENL VEVKFARTPV 250 260 270 280 290 300 MSTYLVAFVV GEYDFVETRS KDGVCVRVYT PVGKAEQGKF ALEVAAKTLP FYKDYFNVPY 310 320 330 340 350 360 PLPKIDLIAI ADFAAGAMEN WGLVTYRETA LLIDPKNSCS SSRQWVALVV GHELAHQWFG 370 380 390 400 410 420 NLVTMEWWTH LWLNEGFASW IEYLCVDHCF PEYDIWTQFV SADYTRAQEL DALDNSHPIE 430 440 450 460 470 480 VSVGHPSEVD EIFDAISYSK GASVIRMLHD YIGDKDFKKG MNMYLTKFQQ KNAATEDLWE 490 500 510 520 530 540 SLENASGKPI AAVMNTWTKQ MGFPLIYVEA EQVEDDRLLR LSQKKFCAGG SYVGEDCPQW 550 560 570 580 590 600 MVPITISTSE DPNQAKLKIL MDKPEMNVVL KNVKPDQWVK LNLGTVGFYR TQYSSAMLES 610 620 630 640 650 660 LLPGIRDLSL PPVDRLGLQN DLFSLARAGI ISTVEVLKVM EAFVNEPNYT VWSDLSCNLG 670 680 690 700 710 720 ILSTLLSHTD FYEEIQEFVK DVFSPIGERL GWDPKPGEGH LDALLRGLVL GKLGKAGHKA 730 740 750 760 770 780 TLEEARRRFK DHVEGKQILS ADLRSPVYLT VLKHGDGTTL DIMLKLHKQA DMQEEKNRIE 790 800 810 820 830 840 RVLGATLLPD LIQKVLTFAL SEEVRPQDTV SVIGGVAGGS KHGRKAAWKF IKDNWEELYN 850 860 870 880 890 900 RYQGGFLISR LIKLSVEGFA VDKMAGEVKA FFESHPAPSA ERTIQQCCEN ILLNAAWLKR 910 DAESIHQYLL QRKASPPTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the human puromycin-sensitive aminopeptidase and evidence for expression in neurons." Tobler A.R., Constam D.B., Schmitt-Graeff A., Malipiero U., Schlapbach R., Fontana A. J. Neurochem. 68:889-897(1997) [PubMed: 9048733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 31-919. Tissue: Lung.
[4]	"Human puromycin-sensitive aminopeptidase: cloning of 3' UTR, evidence for a polymorphism at a.a. 140 and refined chromosomal localization to 17q21." Bauer W.O., Nanda I., Beck G., Schmid M., Jakob F. Cytogenet. Cell Genet. 92:221-224(2001) [PubMed: 11435692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-919. Tissue: Skeletal muscle.
[5]	"Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase." Thompson M.W., Tobler A., Fontana A., Hersh L.B. Biochem. Biophys. Res. Commun. 258:234-240(1999) [PubMed: 10329370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-85.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y07701 mRNA. Translation: CAA68964.1. Different initiation. AC025682 Genomic DNA. No translation available. BC065294 mRNA. Translation: AAH65294.2. Different initiation. AJ132583 mRNA. Translation: CAA10709.1. AF252387 Genomic DNA. Translation: AAF70086.1.
IPI	IPI00026216.
RefSeq	NP_006301.3.
UniGene	Hs.443837
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P55786. 8 interactions.
STRING	P55786.
Protein family/group databases
MEROPS	M01.010.
PTM databases
PhosphoSite	P55786.
Proteomic databases
PeptideAtlas	P55786.
PRIDE	P55786.
Genome annotation databases
Ensembl	ENST00000322157; ENSP00000320324; ENSG00000141279; Homo sapiens. [Genome view]
GeneID	9520.
KEGG	hsa:9520.
UCSC	uc002ilr.2. human.
Organism-specific databases
CTD	9520.
GeneCards	GC17M033612. GC17P042963.
HGNC	HGNC:7900. NPEPPS.
MIM	606793. gene.
PharmGKB	PA31703.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P55786.
HOVERGEN	P55786.
OMA	EYKAFFE
OrthoDB	EOG9909WF
Gene expression databases
ArrayExpress	P55786.
Bgee	P55786.
CleanEx	HS_NPEPPS.
Genevestigator	P55786.
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. IPR015568. Peptidase_M1_puromycin-sens. [Graphical view]
PANTHER	PTHR11533:SF33. Pept_M1_puromysin-sens. 1 hit. PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35678.
PMAP-CutDB	P55786.
SOURCE	Search...

Entry information

Entry name

PSA_HUMAN

Accession

Primary (citable) accession number: P55786
Secondary accession number(s): Q6P145, Q9NP16, Q9UEM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 31, 2004
Last modified:	November 24, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.