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Protein

Heptaprenyl diphosphate synthase component 2

Gene

hepT

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7).

Catalytic activityi

(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451Isopentenyl diphosphateBy similarity
Binding sitei48 – 481Isopentenyl diphosphateBy similarity
Binding sitei77 – 771Isopentenyl diphosphateBy similarity
Metal bindingi84 – 841Magnesium 1By similarity
Metal bindingi84 – 841Magnesium 2By similarity
Metal bindingi88 – 881Magnesium 1By similarity
Metal bindingi88 – 881Magnesium 2By similarity
Binding sitei93 – 931Hexaprenyl diphosphateBy similarity
Binding sitei94 – 941Isopentenyl diphosphateBy similarity
Binding sitei170 – 1701Hexaprenyl diphosphateBy similarity
Binding sitei171 – 1711Hexaprenyl diphosphateBy similarity
Binding sitei208 – 2081Hexaprenyl diphosphateBy similarity
Metal bindingi211 – 2111Magnesium 3By similarity

GO - Molecular functioni

  1. heptaprenyl diphosphate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. transferase activity, transferring alkyl or aryl (other than methyl) groups Source: UniProtKB

GO - Biological processi

  1. isoprenoid biosynthetic process Source: UniProtKB-KW
  2. menaquinone biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.5.1.30. 623.

Names & Taxonomyi

Protein namesi
Recommended name:
Heptaprenyl diphosphate synthase component 2 (EC:2.5.1.30)
Short name:
HepPP synthase subunit 2
Gene namesi
Name:hepT
Synonyms:hepS-2
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Heptaprenyl diphosphate synthase component 2PRO_0000124003Add
BLAST

Interactioni

Subunit structurei

Heterodimer of component I and II.

Structurei

3D structure databases

ProteinModelPortaliP55785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR014119. GerC3_HepT.
IPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR02748. GerC3_HepT. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKAMYSFL SDDLAAVEEE LERAVQSEYG PLGEAALHLL QAGGKRIRPV
60 70 80 90 100
FVLLAARFGQ YDLERMKHVA VALELIHMAS LVHDDVIDDA DLRRGRPTIK
110 120 130 140 150
AKWSNRFAMY TGDYLFARSL ERMAELGNPR AHQVLAKTIV EVCRGEIEQI
160 170 180 190 200
KDKYRFDQPL RTYLRRIRRK TALLIAASCQ LGALAAGAPE PIVKRLYWFG
210 220 230 240 250
HYVGMSFQIT DDILDFTGTE EQLGKPAGSD LLQGNVTLPV LYALSDERVK
260 270 280 290 300
AAIAAVGPET DVAEMAAVIS AIKRTDAIER SYALSDRYLD KALHLLDGLP
310 320
MNEARGLLRD LALYIGKRDY
Length:320
Mass (Da):35,808
Last modified:November 1, 1997 - v1
Checksum:iA51B6630E6B88F46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49976 Genomic DNA. Translation: BAA08725.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49976 Genomic DNA. Translation: BAA08725.1.

3D structure databases

ProteinModelPortaliP55785.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.5.1.30. 623.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR014119. GerC3_HepT.
IPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR02748. GerC3_HepT. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and nucleotide sequences of the genes for two essential proteins constituting a novel enzyme system for heptaprenyl diphosphate synthesis."
    Koike-Takeshita A., Koyama T., Obata S., Ogura K.
    J. Biol. Chem. 270:18396-18400(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10149 / DSM 6790 / JCM 11297 / NRS T15.

Entry informationi

Entry nameiHEPS2_GEOSE
AccessioniPrimary (citable) accession number: P55785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 29, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.