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P55774 (CCL18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 18
Alternative name(s):
Alternative macrophage activation-associated CC chemokine 1
Short name=AMAC-1
CC chemokine PARC
Dendritic cell chemokine 1
Short name=DC-CK1
Macrophage inflammatory protein 4
Short name=MIP-4
Pulmonary and activation-regulated chemokine
Small-inducible cytokine A18

Cleaved into the following 3 chains:

  1. CCL18(1-68)
  2. CCL18(3-69)
  3. CCL18(4-69)
Gene names
Name:CCL18
Synonyms:AMAC1, DCCK1, MIP4, PARC, SCYA18
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length89 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. Ref.13 Ref.14

Subcellular location

Secreted.

Tissue specificity

Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. Ref.13

Induction

Specifically induced in macrophages by IL4/interleukin-4, IL13/interleukin-13, and IL10/interleukin-10. Expression is inhibited by IFNG/IFN-gamma while glucocorticoids exert a slightly positive synergistic effect in combination with IL4/interleukin-4. Strongly induced in several human cell lines, including monocytic U-937 cells, by phorbol myristate acetate (PMA). Induced in PBMC by staphylococcal enterotoxins SEA and SEB. Ref.13

Post-translational modification

The Cys-30/Cys-54 disulfide bond is required for activity.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Mass spectrometry

Molecular mass is 7849.4 Da from positions 21 - 89. Determined by ESI. Ref.13

Molecular mass is 7652.1 Da from positions 23 - 89. Determined by ESI. Ref.13

Molecular mass is 7552.0 Da from positions 24 - 89. Determined by ESI. Ref.13

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PITPNM3Q9BZ714EBI-711240,EBI-2815766

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.4
Chain21 – 8969C-C motif chemokine 18
PRO_0000005212
Chain21 – 8868CCL18(1-68)
PRO_0000041851
Chain23 – 8967CCL18(3-69)
PRO_0000041852
Chain24 – 8966CCL18(4-69)
PRO_0000041853

Amino acid modifications

Disulfide bond30 ↔ 54 Ref.15
Disulfide bond31 ↔ 70 Ref.15

Sequences

Sequence LengthMass (Da)Tools
P55774 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C287B94B9C0518E4

FASTA899,849
        10         20         30         40         50         60 
MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI 

        70         80 
LLTKRGRQIC ADPNKKWVQK YISDLKLNA 

« Hide

References

« Hide 'large scale' references
[1]"Macrophage inflammatory protein-3 and -4."
Li H., Ruben S.
Patent number US5504003, 02-APR-1996
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes."
Hieshima K., Imai T., Baba M., Shoudai K., Ishizuka K., Nakagawa T., Tsuruta J., Takeya M., Sakaki Y., Takatsuki K., Miura R., Opdenakker G., van Damme J., Yoshie O., Nomiyama H.
J. Immunol. 159:1140-1149(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Aorta and Lung.
[3]"Alternative macrophage activation-associated CC-chemokine-1, a novel structural homologue of macrophage inflammatory protein-1 alpha with a Th2-associated expression pattern."
Kodelja V., Mueller C., Politz O., Hakij N., Orfanos C.E., Goerdt S.
J. Immunol. 160:1411-1418(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"A dendritic-cell-derived C-C chemokine that preferentially attracts naive T cells."
Adema G.J., Hartgers F., Verstraten R., de Vries E., Marland G., Menon S., Foster J., Xu Y., Nooyen P., McClanahan T., Bacon K.B., Figdor C.G.
Nature 387:713-717(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS.
Tissue: Dendritic cell.
[5]"Chemokine PARC gene (SCYA18) generated by fusion of two MIP-1alpha/LD78alpha-like genes."
Tasaki Y., Fukuda S., Iio M., Miura R., Imai T., Sugano S., Yoshie O., Hughes A.L., Nomiyama H.
Genomics 55:353-357(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Genomic organization and biological characterization of the novel human CC chemokine DC-CK-1/PARC/MIP-4/SCYA18."
Guan P., Burghes A.H.M., Cunningham A., Lira P., Brissette W.H., Neote K., McColl S.R.
Genomics 56:296-302(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[7]"The genomic locus for the AMAC-1 gene contains possible pseudo-exons within the first intron sequence."
Politz O., Kodelja V., Guillot P., Orfanos C.E., Goerdt S.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. expand/collapse author list , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[12]"The chemokine information source: identification and characterization of novel chemokines using the WorldWideWeb and expressed sequence tag databases."
Wells T.N.C., Peitsch M.C.
J. Leukoc. Biol. 61:545-550(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.
[13]"Selective induction of CCL18/PARC by staphylococcal enterotoxins in mononuclear cells and enhanced levels in septic and rheumatoid arthritis."
Schutyser E., Struyf S., Wuyts A., Put W., Geboes K., Grillet B., Opdenakker G., Van Damme J.
Eur. J. Immunol. 31:3755-3762(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF CCL18(3-69) AND CCL18(4-69), PROTEOLYTIC PROCESSING OF N-TERMINAL, FUNCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY, INDUCTION.
[14]"Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma."
Schutyser E., Struyf S., Proost P., Opdenakker G., Laureys G., Verhasselt B., Peperstraete L., Van de Putte I., Saccani A., Allavena P., Mantovani A., Van Damme J.
J. Biol. Chem. 277:24584-24593(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF CCL18(1-68), PROTEOLYTIC PROCESSING OF C-TERMINAL, FUNCTION.
[15]"The disulfide bond between cysteine 10 and cysteine 34 is required for CCL18 activity."
Legendre B., Tokarski C., Chang Y., De Freitas Caires N., Lortat-Jacob H., Nadai P.D., Rolando C., Duez C., Tsicopoulos A., Lassalle P.
Cytokine 64:463-470(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
+Additional computationally mapped references.

Web resources

Wikipedia

CCL18 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB000221 mRNA. Translation: BAA21670.1.
Y13710 mRNA. Translation: CAA74039.1.
AB012113 Genomic DNA. Translation: BAA34368.1.
AF082214, AF082212, AF082213 Genomic DNA. Translation: AAC32287.1.
AF111198 Genomic DNA. Translation: AAD30390.1.
CR407660 mRNA. Translation: CAG28588.1.
AB451458 mRNA. Translation: BAG70272.1.
CH471147 Genomic DNA. Translation: EAW80102.1.
BC069700 mRNA. Translation: AAH69700.1.
BC096124 mRNA. Translation: AAH96124.1.
BC096125 mRNA. Translation: AAH96125.1.
BC096126 mRNA. Translation: AAH96126.1.
BC096127 mRNA. Translation: AAH96127.1.
CCDSCCDS11306.1.
RefSeqNP_002979.1. NM_002988.2.
UniGeneHs.143961.

3D structure databases

ProteinModelPortalP55774.
SMRP55774. Positions 30-86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112265. 6 interactions.
IntActP55774. 7 interactions.
MINTMINT-1373449.
STRING9606.ENSP00000004921.

Polymorphism databases

DMDM2493666.

Proteomic databases

PaxDbP55774.
PRIDEP55774.

Protocols and materials databases

DNASU6362.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000004921; ENSP00000004921; ENSG00000006074.
GeneID6362.
KEGGhsa:6362.
UCSCuc002hku.3. human.

Organism-specific databases

CTD6362.
GeneCardsGC17P034391.
HGNCHGNC:10616. CCL18.
MIM603757. gene.
neXtProtNX_P55774.
PharmGKBPA35549.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG67988.
HOGENOMHOG000036685.
InParanoidP55774.
KOK05514.
OMAFIVDYSE.
OrthoDBEOG7CVQ1F.
PhylomeDBP55774.
TreeFamTF334888.

Gene expression databases

BgeeP55774.
CleanExHS_AMAC1.
HS_CCL18.
GenevestigatorP55774.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi6362.
NextBio24720.
PROP55774.
SOURCESearch...

Entry information

Entry nameCCL18_HUMAN
AccessionPrimary (citable) accession number: P55774
Secondary accession number(s): B5BUM2, Q53X71
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM