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P55774

- CCL18_HUMAN

UniProt

P55774 - CCL18_HUMAN

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Protein

C-C motif chemokine 18

Gene

CCL18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.2 Publications

GO - Molecular functioni

  1. chemokine activity Source: ProtInc

GO - Biological processi

  1. cell-cell signaling Source: ProtInc
  2. cell chemotaxis Source: GOC
  3. cell communication Source: ProtInc
  4. chemotaxis Source: ProtInc
  5. immune response Source: ProtInc
  6. inflammatory response Source: UniProtKB-KW
  7. response to biotic stimulus Source: ProtInc
  8. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 18
Alternative name(s):
Alternative macrophage activation-associated CC chemokine 1
Short name:
AMAC-1
CC chemokine PARC
Dendritic cell chemokine 1
Short name:
DC-CK1
Macrophage inflammatory protein 4
Short name:
MIP-4
Pulmonary and activation-regulated chemokine
Small-inducible cytokine A18
Cleaved into the following 3 chains:
Gene namesi
Name:CCL18
Synonyms:AMAC1, DCCK1, MIP4, PARC, SCYA18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:10616. CCL18.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35549.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 8969C-C motif chemokine 18PRO_0000005212Add
BLAST
Chaini21 – 8868CCL18(1-68)PRO_0000041851Add
BLAST
Chaini23 – 8967CCL18(3-69)PRO_0000041852Add
BLAST
Chaini24 – 8966CCL18(4-69)PRO_0000041853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 541 Publication
Disulfide bondi31 ↔ 701 Publication

Post-translational modificationi

The Cys-30/Cys-54 disulfide bond is required for activity.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP55774.
PRIDEiP55774.

Expressioni

Tissue specificityi

Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.1 Publication

Inductioni

Specifically induced in macrophages by IL4/interleukin-4, IL13/interleukin-13, and IL10/interleukin-10. Expression is inhibited by IFNG/IFN-gamma while glucocorticoids exert a slightly positive synergistic effect in combination with IL4/interleukin-4. Strongly induced in several human cell lines, including monocytic U-937 cells, by phorbol myristate acetate (PMA). Induced in PBMC by staphylococcal enterotoxins SEA and SEB.1 Publication

Gene expression databases

BgeeiP55774.
CleanExiHS_AMAC1.
HS_CCL18.
GenevestigatoriP55774.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PITPNM3Q9BZ714EBI-711240,EBI-2815766

Protein-protein interaction databases

BioGridi112265. 6 interactions.
IntActiP55774. 7 interactions.
MINTiMINT-1373449.
STRINGi9606.ENSP00000004921.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MHEX-ray2.10A/B/C/D21-89[»]
ProteinModelPortaliP55774.
SMRiP55774. Positions 30-86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG67988.
GeneTreeiENSGT00730000110278.
HOGENOMiHOG000036685.
InParanoidiP55774.
KOiK05514.
OMAiFIVDYSE.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP55774.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55774-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET
60 70 80
SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA
Length:89
Mass (Da):9,849
Last modified:November 1, 1997 - v1
Checksum:iC287B94B9C0518E4
GO

Mass spectrometryi

Molecular mass is 7849.4 Da from positions 21 - 89. Determined by ESI. 1 Publication
Molecular mass is 7652.1 Da from positions 23 - 89. Determined by ESI. 1 Publication
Molecular mass is 7552.0 Da from positions 24 - 89. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB000221 mRNA. Translation: BAA21670.1.
Y13710 mRNA. Translation: CAA74039.1.
AB012113 Genomic DNA. Translation: BAA34368.1.
AF082214, AF082212, AF082213 Genomic DNA. Translation: AAC32287.1.
AF111198 Genomic DNA. Translation: AAD30390.1.
CR407660 mRNA. Translation: CAG28588.1.
AB451458 mRNA. Translation: BAG70272.1.
CH471147 Genomic DNA. Translation: EAW80102.1.
BC069700 mRNA. Translation: AAH69700.1.
BC096124 mRNA. Translation: AAH96124.1.
BC096125 mRNA. Translation: AAH96125.1.
BC096126 mRNA. Translation: AAH96126.1.
BC096127 mRNA. Translation: AAH96127.1.
CCDSiCCDS11306.1.
RefSeqiNP_002979.1. NM_002988.2.
UniGeneiHs.143961.

Genome annotation databases

EnsembliENST00000616054; ENSP00000479955; ENSG00000275385.
GeneIDi6362.
KEGGihsa:6362.
UCSCiuc002hku.3. human.

Polymorphism databases

DMDMi2493666.

Cross-referencesi

Web resourcesi

Wikipedia

CCL18 entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB000221 mRNA. Translation: BAA21670.1 .
Y13710 mRNA. Translation: CAA74039.1 .
AB012113 Genomic DNA. Translation: BAA34368.1 .
AF082214 , AF082212 , AF082213 Genomic DNA. Translation: AAC32287.1 .
AF111198 Genomic DNA. Translation: AAD30390.1 .
CR407660 mRNA. Translation: CAG28588.1 .
AB451458 mRNA. Translation: BAG70272.1 .
CH471147 Genomic DNA. Translation: EAW80102.1 .
BC069700 mRNA. Translation: AAH69700.1 .
BC096124 mRNA. Translation: AAH96124.1 .
BC096125 mRNA. Translation: AAH96125.1 .
BC096126 mRNA. Translation: AAH96126.1 .
BC096127 mRNA. Translation: AAH96127.1 .
CCDSi CCDS11306.1.
RefSeqi NP_002979.1. NM_002988.2.
UniGenei Hs.143961.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MHE X-ray 2.10 A/B/C/D 21-89 [» ]
ProteinModelPortali P55774.
SMRi P55774. Positions 30-86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112265. 6 interactions.
IntActi P55774. 7 interactions.
MINTi MINT-1373449.
STRINGi 9606.ENSP00000004921.

Polymorphism databases

DMDMi 2493666.

Proteomic databases

PaxDbi P55774.
PRIDEi P55774.

Protocols and materials databases

DNASUi 6362.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000616054 ; ENSP00000479955 ; ENSG00000275385 .
GeneIDi 6362.
KEGGi hsa:6362.
UCSCi uc002hku.3. human.

Organism-specific databases

CTDi 6362.
GeneCardsi GC17P034391.
HGNCi HGNC:10616. CCL18.
MIMi 603757. gene.
neXtProti NX_P55774.
PharmGKBi PA35549.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG67988.
GeneTreei ENSGT00730000110278.
HOGENOMi HOG000036685.
InParanoidi P55774.
KOi K05514.
OMAi FIVDYSE.
OrthoDBi EOG7CVQ1F.
PhylomeDBi P55774.
TreeFami TF334888.

Miscellaneous databases

GenomeRNAii 6362.
NextBioi 24720.
PROi P55774.
SOURCEi Search...

Gene expression databases

Bgeei P55774.
CleanExi HS_AMAC1.
HS_CCL18.
Genevestigatori P55774.

Family and domain databases

InterProi IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view ]
Pfami PF00048. IL8. 1 hit.
[Graphical view ]
SMARTi SM00199. SCY. 1 hit.
[Graphical view ]
SUPFAMi SSF54117. SSF54117. 1 hit.
PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Macrophage inflammatory protein-3 and -4."
    Li H., Ruben S.
    Patent number US5504003, 02-APR-1996
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes."
    Hieshima K., Imai T., Baba M., Shoudai K., Ishizuka K., Nakagawa T., Tsuruta J., Takeya M., Sakaki Y., Takatsuki K., Miura R., Opdenakker G., van Damme J., Yoshie O., Nomiyama H.
    J. Immunol. 159:1140-1149(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Aorta and Lung.
  3. "Alternative macrophage activation-associated CC-chemokine-1, a novel structural homologue of macrophage inflammatory protein-1 alpha with a Th2-associated expression pattern."
    Kodelja V., Mueller C., Politz O., Hakij N., Orfanos C.E., Goerdt S.
    J. Immunol. 160:1411-1418(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "A dendritic-cell-derived C-C chemokine that preferentially attracts naive T cells."
    Adema G.J., Hartgers F., Verstraten R., de Vries E., Marland G., Menon S., Foster J., Xu Y., Nooyen P., McClanahan T., Bacon K.B., Figdor C.G.
    Nature 387:713-717(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS.
    Tissue: Dendritic cell.
  5. "Chemokine PARC gene (SCYA18) generated by fusion of two MIP-1alpha/LD78alpha-like genes."
    Tasaki Y., Fukuda S., Iio M., Miura R., Imai T., Sugano S., Yoshie O., Hughes A.L., Nomiyama H.
    Genomics 55:353-357(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Genomic organization and biological characterization of the novel human CC chemokine DC-CK-1/PARC/MIP-4/SCYA18."
    Guan P., Burghes A.H.M., Cunningham A., Lira P., Brissette W.H., Neote K., McColl S.R.
    Genomics 56:296-302(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  7. "The genomic locus for the AMAC-1 gene contains possible pseudo-exons within the first intron sequence."
    Politz O., Kodelja V., Guillot P., Orfanos C.E., Goerdt S.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  10. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
    Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B.
    , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
    Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  12. "The chemokine information source: identification and characterization of novel chemokines using the WorldWideWeb and expressed sequence tag databases."
    Wells T.N.C., Peitsch M.C.
    J. Leukoc. Biol. 61:545-550(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  13. "Selective induction of CCL18/PARC by staphylococcal enterotoxins in mononuclear cells and enhanced levels in septic and rheumatoid arthritis."
    Schutyser E., Struyf S., Wuyts A., Put W., Geboes K., Grillet B., Opdenakker G., Van Damme J.
    Eur. J. Immunol. 31:3755-3762(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CCL18(3-69) AND CCL18(4-69), PROTEOLYTIC PROCESSING OF N-TERMINAL, FUNCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY, INDUCTION.
  14. "Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma."
    Schutyser E., Struyf S., Proost P., Opdenakker G., Laureys G., Verhasselt B., Peperstraete L., Van de Putte I., Saccani A., Allavena P., Mantovani A., Van Damme J.
    J. Biol. Chem. 277:24584-24593(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CCL18(1-68), PROTEOLYTIC PROCESSING OF C-TERMINAL, FUNCTION.
  15. "The disulfide bond between cysteine 10 and cysteine 34 is required for CCL18 activity."
    Legendre B., Tokarski C., Chang Y., De Freitas Caires N., Lortat-Jacob H., Nadai P.D., Rolando C., Duez C., Tsicopoulos A., Lassalle P.
    Cytokine 64:463-470(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiCCL18_HUMAN
AccessioniPrimary (citable) accession number: P55774
Secondary accession number(s): B5BUM2, Q53X71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3