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P55774

- CCL18_HUMAN

UniProt

P55774 - CCL18_HUMAN

Protein

C-C motif chemokine 18

Gene

CCL18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.2 Publications

    GO - Molecular functioni

    1. chemokine activity Source: ProtInc
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. cell chemotaxis Source: GOC
    3. cell communication Source: ProtInc
    4. chemotaxis Source: ProtInc
    5. immune response Source: ProtInc
    6. inflammatory response Source: UniProtKB-KW
    7. response to biotic stimulus Source: ProtInc
    8. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Chemotaxis, Inflammatory response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C motif chemokine 18
    Alternative name(s):
    Alternative macrophage activation-associated CC chemokine 1
    Short name:
    AMAC-1
    CC chemokine PARC
    Dendritic cell chemokine 1
    Short name:
    DC-CK1
    Macrophage inflammatory protein 4
    Short name:
    MIP-4
    Pulmonary and activation-regulated chemokine
    Small-inducible cytokine A18
    Cleaved into the following 3 chains:
    Gene namesi
    Name:CCL18
    Synonyms:AMAC1, DCCK1, MIP4, PARC, SCYA18
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:10616. CCL18.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35549.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20201 PublicationAdd
    BLAST
    Chaini21 – 8969C-C motif chemokine 18PRO_0000005212Add
    BLAST
    Chaini21 – 8868CCL18(1-68)PRO_0000041851Add
    BLAST
    Chaini23 – 8967CCL18(3-69)PRO_0000041852Add
    BLAST
    Chaini24 – 8966CCL18(4-69)PRO_0000041853Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 541 Publication
    Disulfide bondi31 ↔ 701 Publication

    Post-translational modificationi

    The Cys-30/Cys-54 disulfide bond is required for activity.

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP55774.
    PRIDEiP55774.

    Expressioni

    Tissue specificityi

    Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.1 Publication

    Inductioni

    Specifically induced in macrophages by IL4/interleukin-4, IL13/interleukin-13, and IL10/interleukin-10. Expression is inhibited by IFNG/IFN-gamma while glucocorticoids exert a slightly positive synergistic effect in combination with IL4/interleukin-4. Strongly induced in several human cell lines, including monocytic U-937 cells, by phorbol myristate acetate (PMA). Induced in PBMC by staphylococcal enterotoxins SEA and SEB.1 Publication

    Gene expression databases

    BgeeiP55774.
    CleanExiHS_AMAC1.
    HS_CCL18.
    GenevestigatoriP55774.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PITPNM3Q9BZ714EBI-711240,EBI-2815766

    Protein-protein interaction databases

    BioGridi112265. 6 interactions.
    IntActiP55774. 7 interactions.
    MINTiMINT-1373449.
    STRINGi9606.ENSP00000004921.

    Structurei

    3D structure databases

    ProteinModelPortaliP55774.
    SMRiP55774. Positions 30-86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG67988.
    HOGENOMiHOG000036685.
    InParanoidiP55774.
    KOiK05514.
    OMAiFIVDYSE.
    OrthoDBiEOG7CVQ1F.
    PhylomeDBiP55774.
    TreeFamiTF334888.

    Family and domain databases

    InterProiIPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P55774-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET   50
    SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA 89
    Length:89
    Mass (Da):9,849
    Last modified:November 1, 1997 - v1
    Checksum:iC287B94B9C0518E4
    GO

    Mass spectrometryi

    Molecular mass is 7849.4 Da from positions 21 - 89. Determined by ESI. 1 Publication
    Molecular mass is 7652.1 Da from positions 23 - 89. Determined by ESI. 1 Publication
    Molecular mass is 7552.0 Da from positions 24 - 89. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000221 mRNA. Translation: BAA21670.1.
    Y13710 mRNA. Translation: CAA74039.1.
    AB012113 Genomic DNA. Translation: BAA34368.1.
    AF082214, AF082212, AF082213 Genomic DNA. Translation: AAC32287.1.
    AF111198 Genomic DNA. Translation: AAD30390.1.
    CR407660 mRNA. Translation: CAG28588.1.
    AB451458 mRNA. Translation: BAG70272.1.
    CH471147 Genomic DNA. Translation: EAW80102.1.
    BC069700 mRNA. Translation: AAH69700.1.
    BC096124 mRNA. Translation: AAH96124.1.
    BC096125 mRNA. Translation: AAH96125.1.
    BC096126 mRNA. Translation: AAH96126.1.
    BC096127 mRNA. Translation: AAH96127.1.
    CCDSiCCDS11306.1.
    RefSeqiNP_002979.1. NM_002988.2.
    UniGeneiHs.143961.

    Genome annotation databases

    GeneIDi6362.
    KEGGihsa:6362.
    UCSCiuc002hku.3. human.

    Polymorphism databases

    DMDMi2493666.

    Cross-referencesi

    Web resourcesi

    Wikipedia

    CCL18 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000221 mRNA. Translation: BAA21670.1 .
    Y13710 mRNA. Translation: CAA74039.1 .
    AB012113 Genomic DNA. Translation: BAA34368.1 .
    AF082214 , AF082212 , AF082213 Genomic DNA. Translation: AAC32287.1 .
    AF111198 Genomic DNA. Translation: AAD30390.1 .
    CR407660 mRNA. Translation: CAG28588.1 .
    AB451458 mRNA. Translation: BAG70272.1 .
    CH471147 Genomic DNA. Translation: EAW80102.1 .
    BC069700 mRNA. Translation: AAH69700.1 .
    BC096124 mRNA. Translation: AAH96124.1 .
    BC096125 mRNA. Translation: AAH96125.1 .
    BC096126 mRNA. Translation: AAH96126.1 .
    BC096127 mRNA. Translation: AAH96127.1 .
    CCDSi CCDS11306.1.
    RefSeqi NP_002979.1. NM_002988.2.
    UniGenei Hs.143961.

    3D structure databases

    ProteinModelPortali P55774.
    SMRi P55774. Positions 30-86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112265. 6 interactions.
    IntActi P55774. 7 interactions.
    MINTi MINT-1373449.
    STRINGi 9606.ENSP00000004921.

    Polymorphism databases

    DMDMi 2493666.

    Proteomic databases

    PaxDbi P55774.
    PRIDEi P55774.

    Protocols and materials databases

    DNASUi 6362.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6362.
    KEGGi hsa:6362.
    UCSCi uc002hku.3. human.

    Organism-specific databases

    CTDi 6362.
    GeneCardsi GC17P034391.
    HGNCi HGNC:10616. CCL18.
    MIMi 603757. gene.
    neXtProti NX_P55774.
    PharmGKBi PA35549.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG67988.
    HOGENOMi HOG000036685.
    InParanoidi P55774.
    KOi K05514.
    OMAi FIVDYSE.
    OrthoDBi EOG7CVQ1F.
    PhylomeDBi P55774.
    TreeFami TF334888.

    Miscellaneous databases

    GenomeRNAii 6362.
    NextBioi 24720.
    PROi P55774.
    SOURCEi Search...

    Gene expression databases

    Bgeei P55774.
    CleanExi HS_AMAC1.
    HS_CCL18.
    Genevestigatori P55774.

    Family and domain databases

    InterProi IPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Macrophage inflammatory protein-3 and -4."
      Li H., Ruben S.
      Patent number US5504003, 02-APR-1996
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes."
      Hieshima K., Imai T., Baba M., Shoudai K., Ishizuka K., Nakagawa T., Tsuruta J., Takeya M., Sakaki Y., Takatsuki K., Miura R., Opdenakker G., van Damme J., Yoshie O., Nomiyama H.
      J. Immunol. 159:1140-1149(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Aorta and Lung.
    3. "Alternative macrophage activation-associated CC-chemokine-1, a novel structural homologue of macrophage inflammatory protein-1 alpha with a Th2-associated expression pattern."
      Kodelja V., Mueller C., Politz O., Hakij N., Orfanos C.E., Goerdt S.
      J. Immunol. 160:1411-1418(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "A dendritic-cell-derived C-C chemokine that preferentially attracts naive T cells."
      Adema G.J., Hartgers F., Verstraten R., de Vries E., Marland G., Menon S., Foster J., Xu Y., Nooyen P., McClanahan T., Bacon K.B., Figdor C.G.
      Nature 387:713-717(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS.
      Tissue: Dendritic cell.
    5. "Chemokine PARC gene (SCYA18) generated by fusion of two MIP-1alpha/LD78alpha-like genes."
      Tasaki Y., Fukuda S., Iio M., Miura R., Imai T., Sugano S., Yoshie O., Hughes A.L., Nomiyama H.
      Genomics 55:353-357(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "Genomic organization and biological characterization of the novel human CC chemokine DC-CK-1/PARC/MIP-4/SCYA18."
      Guan P., Burghes A.H.M., Cunningham A., Lira P., Brissette W.H., Neote K., McColl S.R.
      Genomics 56:296-302(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    7. "The genomic locus for the AMAC-1 gene contains possible pseudo-exons within the first intron sequence."
      Politz O., Kodelja V., Guillot P., Orfanos C.E., Goerdt S.
      Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    10. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
      Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B.
      , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
      Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    12. "The chemokine information source: identification and characterization of novel chemokines using the WorldWideWeb and expressed sequence tag databases."
      Wells T.N.C., Peitsch M.C.
      J. Leukoc. Biol. 61:545-550(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.
    13. "Selective induction of CCL18/PARC by staphylococcal enterotoxins in mononuclear cells and enhanced levels in septic and rheumatoid arthritis."
      Schutyser E., Struyf S., Wuyts A., Put W., Geboes K., Grillet B., Opdenakker G., Van Damme J.
      Eur. J. Immunol. 31:3755-3762(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF CCL18(3-69) AND CCL18(4-69), PROTEOLYTIC PROCESSING OF N-TERMINAL, FUNCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY, INDUCTION.
    14. "Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma."
      Schutyser E., Struyf S., Proost P., Opdenakker G., Laureys G., Verhasselt B., Peperstraete L., Van de Putte I., Saccani A., Allavena P., Mantovani A., Van Damme J.
      J. Biol. Chem. 277:24584-24593(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF CCL18(1-68), PROTEOLYTIC PROCESSING OF C-TERMINAL, FUNCTION.
    15. "The disulfide bond between cysteine 10 and cysteine 34 is required for CCL18 activity."
      Legendre B., Tokarski C., Chang Y., De Freitas Caires N., Lortat-Jacob H., Nadai P.D., Rolando C., Duez C., Tsicopoulos A., Lassalle P.
      Cytokine 64:463-470(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.

    Entry informationi

    Entry nameiCCL18_HUMAN
    AccessioniPrimary (citable) accession number: P55774
    Secondary accession number(s): B5BUM2, Q53X71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3