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P55773 (CCL23_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 23
Alternative name(s):
CK-beta-8
Short name=CKB-8
Macrophage inflammatory protein 3
Short name=MIP-3
Myeloid progenitor inhibitory factor 1
Short name=MPIF-1
Small-inducible cytokine A23

Cleaved into the following 4 chains:

  1. CCL23(19-99)
  2. CCL23(22-99)
  3. CCL23(27-99)
  4. CCL23(30-99)
Gene names
Name:CCL23
Synonyms:MIP3, MPIF1, SCYA23
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Ref.9

Subcellular location

Secreted.

Tissue specificity

High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. Ref.9

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
   Biological processChemotaxis
Inflammatory response
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   LigandHeparin-binding
   Molecular functionCytokine
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 9886417. Source: ProtInc

cell-cell signaling

Traceable author statement Ref.2. Source: ProtInc

cellular calcium ion homeostasis

Traceable author statement PubMed 9886417. Source: ProtInc

chemotaxis

Traceable author statement Ref.2. Source: ProtInc

immune response

Traceable author statement Ref.2. Source: ProtInc

inflammatory response

Traceable author statement Ref.2. Source: ProtInc

monocyte chemotaxis

Inferred by curator PubMed 10660125. Source: UniProtKB

negative regulation of C-C chemokine binding

Inferred from direct assay PubMed 10660125. Source: UniProtKB

negative regulation of cell proliferation

Traceable author statement Ref.2. Source: ProtInc

signal transduction

Non-traceable author statement PubMed 9886417. Source: ProtInc

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionCCR1 chemokine receptor binding

Inferred from physical interaction PubMed 10660125. Source: UniProtKB

chemokine activity

Inferred from direct assay PubMed 10660125. Source: UniProtKB

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Short (identifier: P55773-1)

Also known as: CKB-8;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Long (identifier: P55773-2)

Also known as: CKB-8-1;

The sequence of this isoform differs from the canonical sequence as follows:
     46-46: R → MLWRRKIGPQMTLSHAAG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.2
Chain22 – 12099C-C motif chemokine 23
PRO_0000005229
Chain40 – 12081CCL23(19-99)
PRO_0000041847
Chain43 – 12078CCL23(22-99)
PRO_0000041848
Chain48 – 12073CCL23(27-99)
PRO_0000041849
Chain51 – 12070CCL23(30-99)
PRO_0000041850

Amino acid modifications

Disulfide bond54 ↔ 78
Disulfide bond55 ↔ 94
Disulfide bond65 ↔ 105

Natural variations

Alternative sequence461R → MLWRRKIGPQMTLSHAAG in isoform Long.
VSP_001063
Natural variant1061V → M. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs1003645 [ dbSNP | Ensembl ].
VAR_011916

Experimental info

Sequence conflict12 – 4433MLVTA…NPVLL → HAFLLPLVPGPGHKRCRDRV HECQSFHWKIQYFW Ref.1

Secondary structure

................. 120
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Short (CKB-8) [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 6CA622DD3A4B3A1A

FASTA12013,411
        10         20         30         40         50         60 
MKVSVAALSC LMLVTALGSQ ARVTKDAETE FMMSKLPLEN PVLLDRFHAT SADCCISYTP 

        70         80         90        100        110        120 
RSIPCSLLES YFETNSECSK PGVIFLTKKG RRFCANPSDK QVQVCVRMLK LDTRIKTRKN 

« Hide

Isoform Long (CKB-8-1) [UniParc].

Checksum: 932413D6B2E52C5D
Show »

FASTA13715,290

References

« Hide 'large scale' references
[1]"Macrophage inflammatory protein-3 and -4."
Li H., Ruben S.
Patent number US5504003, 02-APR-1996
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-106.
[2]"Molecular and functional characterization of two novel human C-C chemokines as inhibitors of two distinct classes of myeloid progenitors."
Patel V.P., Kreider B.L., Li Y., Li H., Leung K., Salcedo T., Nardelli B., Pippalla V., Gentz S., Thotakura R., Parmelee D., Gentz R., Garotta G.
J. Exp. Med. 185:1163-1172(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), PROTEIN SEQUENCE OF 22-36, VARIANT MET-106.
Tissue: Aortic endothelium.
[3]"Characterization of CKbeta8 and CKbeta8-1: two alternatively spliced forms of human beta-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1."
Youn B.-S., Zhang S.M., Broxmeyer H.E., Cooper S., Antol K., Fraser M. Jr., Kwon B.S.
Blood 91:3118-3126(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SHORT AND LONG), ALTERNATIVE SPLICING, VARIANT MET-106.
Tissue: Monocyte.
[4]"Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, LEC, and RANTES."
Nomiyama H., Fukuda S., Iio M., Tanase S., Miura R., Yoshie O.
J. Interferon Cytokine Res. 19:227-234(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, VARIANT MET-106.
[5]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-106.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT), VARIANT MET-106.
Tissue: Liver.
[8]"The chemokine information source: identification and characterization of novel chemokines using the WorldWideWeb and expressed sequence tag databases."
Wells T.N.C., Peitsch M.C.
J. Leukoc. Biol. 61:545-550(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.
[9]"Proteolytic activation of alternative CCR1 ligands in inflammation."
Berahovich R.D., Miao Z., Wang Y., Premack B., Howard M.C., Schall T.J.
J. Immunol. 174:7341-7351(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF CCL23(19-99); CCL23(22-99); CCL23(27-99) AND CCL23(30-99), PROTEOLYTIC PROCESSING OF N-TERMINUS, TISSUE SPECIFICITY, FUNCTION.
[10]"Solution structure and dynamics of myeloid progenitor inhibitory factor-1 (MPIF-1), a novel monomeric CC chemokine."
Rajarathnam K., Li Y., Rohrer T., Gentz R.
J. Biol. Chem. 276:4909-4916(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 45-120.
+Additional computationally mapped references.

Web resources

Wikipedia

CCL23 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U85767 mRNA. Translation: AAB51134.1.
U58913 mRNA. Translation: AAD10846.1.
U67128 mRNA. Translation: AAD00161.1.
AF088219 Genomic DNA. Translation: AAC63326.1.
AF088219 Genomic DNA. Translation: AAC63327.1.
AC069363 Genomic DNA. No translation available.
CH471147 Genomic DNA. Translation: EAW80103.1.
BC093970 mRNA. Translation: AAH93970.1.
BC143310 mRNA. Translation: AAI43311.1.
RefSeqNP_005055.3. NM_005064.5. [P55773-2]
NP_665905.2. NM_145898.3. [P55773-1]
UniGeneHs.169191.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G91NMR-A45-120[»]
ProteinModelPortalP55773.
SMRP55773. Positions 44-120.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112271. 1 interaction.
STRING9606.ENSP00000293280.

Chemistry

DrugBankDB00374. Treprostinil.

PTM databases

PhosphoSiteP55773.

Polymorphism databases

DMDM308153623.

Proteomic databases

PaxDbP55773.
PRIDEP55773.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000293280; ENSP00000293280; ENSG00000167236. [P55773-2]
ENST00000591423; ENSP00000465954; ENSG00000167236. [P55773-1]
GeneID6368.
KEGGhsa:6368.
UCSCuc002hks.1. human. [P55773-2]
uc002hkt.1. human. [P55773-1]

Organism-specific databases

CTD6368.
GeneCardsGC17M034340.
HGNCHGNC:10622. CCL23.
HPAHPA042015.
MIM602494. gene.
neXtProtNX_P55773.
PharmGKBPA35554.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG136395.
HOGENOMHOG000036685.
HOVERGENHBG017871.
InParanoidP55773.
KOK05511.
OMAFMEDYFE.
OrthoDBEOG7CVQ1F.
PhylomeDBP55773.
TreeFamTF334888.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP55773.
CleanExHS_CCL23.
GenevestigatorP55773.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP55773.
GenomeRNAi6368.
NextBio24742.
PROP55773.
SOURCESearch...

Entry information

Entry nameCCL23_HUMAN
AccessionPrimary (citable) accession number: P55773
Secondary accession number(s): B7ZKQ3 expand/collapse secondary AC list , O00174, O75950, Q52LD4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 5, 2010
Last modified: July 9, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM