P55773 (CCL23_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C motif chemokine 23

Alternative name(s):
CK-beta-8
Short name=CKB-8
Macrophage inflammatory protein 3
Short name=MIP-3
Myeloid progenitor inhibitory factor 1
Short name=MPIF-1
Small-inducible cytokine A23

Cleaved into the following 4 chains:

1. CCL23(19-99)
2. CCL23(22-99)
3. CCL23(27-99)
4. CCL23(30-99)

Gene names

Name:	CCL23
Synonyms:	MIP3, MPIF1, SCYA23

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Ref.9
Subcellular location	Secreted.
Tissue specificity	High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. Ref.9
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Heparin-binding
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 9886417. Source: ProtInc cell-cell signaling Traceable author statement Ref.2. Source: ProtInc cellular calcium ion homeostasis Traceable author statement PubMed 9886417. Source: ProtInc immune response Traceable author statement Ref.2. Source: ProtInc inflammatory response Traceable author statement Ref.2. Source: ProtInc monocyte chemotaxis Inferred by curator PubMed 10660125. Source: UniProtKB negative regulation of C-C chemokine binding Inferred from direct assay PubMed 10660125. Source: UniProtKB negative regulation of cell proliferation Traceable author statement Ref.2. Source: ProtInc
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chemokine activity Inferred from direct assay PubMed 10660125. Source: UniProtKB heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Short (identifier: P55773-1)

Also known as: CKB-8;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Long (identifier: P55773-2)

Also known as: CKB-8-1;

The sequence of this isoform differs from the canonical sequence as follows:
     46-46: R → MLWRRKIGPQMTLSHAAG

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.2

Chain

22 – 120

99

C-C motif chemokine 23

PRO_0000005229

Chain

40 – 120

81

CCL23(19-99)

PRO_0000041847

Chain

43 – 120

78

CCL23(22-99)

PRO_0000041848

Chain

48 – 120

73

CCL23(27-99)

PRO_0000041849

Chain

51 – 120

70

CCL23(30-99)

PRO_0000041850

Amino acid modifications

Disulfide bond

54 ↔ 78

Disulfide bond

55 ↔ 94

Disulfide bond

65 ↔ 105

Natural variations

Alternative sequence

46

1

R → MLWRRKIGPQMTLSHAAG in isoform Long.

VSP_001063

Natural variant

106

1

V → M. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs1003645 [ dbSNP | Ensembl ].

VAR_011916

Experimental info

Sequence conflict

12 – 44

33

MLVTA…NPVLL → HAFLLPLVPGPGHKRCRDRV HECQSFHWKIQYFW Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

120

Helix Strand Turn

Details...

Beta strand

48 – 50

3

Helix

65 – 67

3

Beta strand

70 – 73

4

Beta strand

78 – 80

3

Beta strand

83 – 86

4

Beta strand

92 – 95

4

Helix

100 – 109

10

Turn

113 – 115

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Short (CKB-8) [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 6CA622DD3A4B3A1A	FASTA	120	13,411
	10 20 30 40 50 60 MKVSVAALSC LMLVTALGSQ ARVTKDAETE FMMSKLPLEN PVLLDRFHAT SADCCISYTP 70 80 90 100 110 120 RSIPCSLLES YFETNSECSK PGVIFLTKKG RRFCANPSDK QVQVCVRMLK LDTRIKTRKN
	Isoform Long (CKB-8-1) [UniParc]. Checksum: 932413D6B2E52C5D	FASTA	137	15,290
	10 20 30 40 50 60 MKVSVAALSC LMLVTALGSQ ARVTKDAETE FMMSKLPLEN PVLLDMLWRR KIGPQMTLSH 70 80 90 100 110 120 AAGFHATSAD CCISYTPRSI PCSLLESYFE TNSECSKPGV IFLTKKGRRF CANPSDKQVQ 130 VCVRMLKLDT RIKTRKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Macrophage inflammatory protein-3 and -4." Li H., Ruben S. Patent number US5504003, 02-APR-1996 Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-106.
[2]	"Molecular and functional characterization of two novel human C-C chemokines as inhibitors of two distinct classes of myeloid progenitors." Patel V.P., Kreider B.L., Li Y., Li H., Leung K., Salcedo T., Nardelli B., Pippalla V., Gentz S., Thotakura R., Parmelee D., Gentz R., Garotta G. J. Exp. Med. 185:1163-1172(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), PROTEIN SEQUENCE OF 22-36, VARIANT MET-106. Tissue: Aortic endothelium.
[3]	"Characterization of CKbeta8 and CKbeta8-1: two alternatively spliced forms of human beta-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1." Youn B.-S., Zhang S.M., Broxmeyer H.E., Cooper S., Antol K., Fraser M. Jr., Kwon B.S. Blood 91:3118-3126(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SHORT AND LONG), ALTERNATIVE SPLICING, VARIANT MET-106. Tissue: Monocyte.
[4]	"Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, LEC, and RANTES." Nomiyama H., Fukuda S., Iio M., Tanase S., Miura R., Yoshie O. J. Interferon Cytokine Res. 19:227-234(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, VARIANT MET-106.
[5]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-106.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT), VARIANT MET-106. Tissue: Liver.
[8]	"The chemokine information source: identification and characterization of novel chemokines using the WorldWideWeb and expressed sequence tag databases." Wells T.N.C., Peitsch M.C. J. Leukoc. Biol. 61:545-550(1997) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[9]	"Proteolytic activation of alternative CCR1 ligands in inflammation." Berahovich R.D., Miao Z., Wang Y., Premack B., Howard M.C., Schall T.J. J. Immunol. 174:7341-7351(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF CCL23(19-99); CCL23(22-99); CCL23(27-99) AND CCL23(30-99), PROTEOLYTIC PROCESSING OF N-TERMINUS, TISSUE SPECIFICITY, FUNCTION.
[10]	"Solution structure and dynamics of myeloid progenitor inhibitory factor-1 (MPIF-1), a novel monomeric CC chemokine." Rajarathnam K., Li Y., Rohrer T., Gentz R. J. Biol. Chem. 276:4909-4916(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 45-120.
+	Additional computationally mapped references.

Web resources

Wikipedia

CCL23 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U85767 mRNA. Translation: AAB51134.1.
U58913 mRNA. Translation: AAD10846.1.
U67128 mRNA. Translation: AAD00161.1.
AF088219 Genomic DNA. Translation: AAC63326.1.
AF088219 Genomic DNA. Translation: AAC63327.1.
AC069363 Genomic DNA. No translation available.
CH471147 Genomic DNA. Translation: EAW80103.1.
BC093970 mRNA. Translation: AAH93970.1.
BC143310 mRNA. Translation: AAI43311.1.

IPI

IPI00026179.
IPI00221165.

RefSeq

NP_005055.2. NM_005064.3.
NP_665905.1. NM_145898.1.

UniGene

Hs.169191.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G91	NMR	-	A	45-120	[»]

ProteinModelPortal

P55773.

SMR

P55773. Positions 44-120.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000293280.

PTM databases

PhosphoSite

P55773.

Polymorphism databases

DMDM

308153623.

Proteomic databases

PaxDb

P55773.

PRIDE

P55773.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000293280; ENSP00000293280; ENSG00000167236.
ENST00000591423; ENSP00000465954; ENSG00000167236.

GeneID

6368.

KEGG

hsa:6368.

UCSC

uc002hks.1. human.
uc002hkt.1. human.

Organism-specific databases

CTD

6368.

GeneCards

GC17M034340.

HGNC

HGNC:10622. CCL23.

HPA

HPA042015.

MIM

602494. gene.

neXtProt

NX_P55773.

PharmGKB

PA35554.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG136395.

HOGENOM

HOG000036685.

HOVERGEN

HBG017871.

InParanoid

P55773.

KO

K05511.

OMA

CISYTPR.

OrthoDB

EOG49PB11.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P55773.

CleanEx

HS_CCL23.

Genevestigator

P55773.

GermOnline

ENSG00000167236. Homo sapiens.

Family and domain databases

InterPro

IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00374. Treprostinil.

EvolutionaryTrace

P55773.

GenomeRNAi

6368.

NextBio

24742.

SOURCE

Search...

Entry information

Entry name

CCL23_HUMAN

Accession

Primary (citable) accession number: P55773
Secondary accession number(s): B7ZKQ3 , O00174, O75950, Q52LD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.