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Protein

Serine carboxypeptidase II-2

Gene

CXP;2-2

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491By similarity
Active sitei350 – 3501By similarity
Active sitei403 – 4031By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERihorvu-cp22. Carboxypeptidase_S10.
MEROPSiS10.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase II-2 (EC:3.4.16.6)
Alternative name(s):
CP-MII.2
Cleaved into the following 2 chains:
Gene namesi
Name:CXP;2-2
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Organism-specific databases

GrameneiP55748.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 256›256Serine carboxypeptidase II-2 chain APRO_0000004317Add
BLAST
Propeptidei257 – 27014Linker peptideBy similarityPRO_0000004318Add
BLAST
Chaini271 – 436166Serine carboxypeptidase II-2 chain BPRO_0000004319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 313Interchain (between A and B chains)By similarity
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi217 ↔ 229By similarity
Disulfide bondi253 ↔ 281Interchain (between A and B chains)By similarity

Post-translational modificationi

The linker peptide is endoproteolytically excised during enzyme maturation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

Expressed in the germinating embryo. Low levels in the developing aleurone and embryo. Also found in the roots and shoots of the growing seedling.

Interactioni

Subunit structurei

Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi4513.MLOC_36826.2.

Structurei

3D structure databases

ProteinModelPortaliP55748.
SMRiP55748. Positions 1-254, 278-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
VPRVPGQAFD ASFAHYAGYV TVSEDRGAAL FYWFFEAAHD PASKPLLLWL
60 70 80 90 100
NGGPGCSSIA FGVGEEVGPF HVNADGKGVH MNPYSWNQVA NILFLDSPVG
110 120 130 140 150
VGYSYSNTSA DILSNGDERT AKDSLVFLTK WLERFPQYKE REFYLTGESY
160 170 180 190 200
AGHYVPQLAQ AIKRHHEATG DKSINLKGYM VGNALTDDFH DHYGIFQYMW
210 220 230 240 250
TTGLISDQTY KLLNIFCDFE SFVHTSPQCD KILDIASTEA GNIDSYSIFT
260 270 280 290 300
PTCHSSFASS RNKVVKRLRS VGKMGEQYDP CTEKHSIVYF NLHEVQKALH
310 320 330 340 350
VNPVIGKSKW ETCSEVINTN WKDCERSVLH IYHELIQYGL RIWMFSGDTD
360 370 380 390 400
AVIPVTSTRY SIDALKLPTV TPWHAWYDDD GEVGGWTQGY KGLNFVTVRG
410 420 430
AGHEVPLHRP KQALTLIKSF LAGRPMPVLS DLRSDM
Length:436
Mass (Da):48,952
Last modified:November 1, 1997 - v1
Checksum:iE0F82D97E0C34DC9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78878 mRNA. Translation: CAB59202.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78878 mRNA. Translation: CAB59202.1.

3D structure databases

ProteinModelPortaliP55748.
SMRiP55748. Positions 1-254, 278-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4513.MLOC_36826.2.

Protein family/group databases

ESTHERihorvu-cp22. Carboxypeptidase_S10.
MEROPSiS10.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP55748.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The expression of serine carboxypeptidases during maturation and germination of the barley grain."
    Dal Degan F., Rocher A., Cameron-Mills V., von Wettstein D.
    Proc. Natl. Acad. Sci. U.S.A. 91:8209-8213(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Alexis.
    Tissue: Grain.

Entry informationi

Entry nameiCBP22_HORVU
AccessioniPrimary (citable) accession number: P55748
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.