Reviewed, UniProtKB/Swiss-Prot P55708 (Y4XP_RHISN)

Names and origin

Protein names

Recommended name:
    Putative cysteine synthase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
      Short name=CSase
    O-acetylserine (Thiol)-lyase

Gene names

Ordered Locus Names:	NGR_a00730
ORF Names:	y4xP

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	As it is highly similar to bacterial and plant cysteine synthases, it is possible that it catalyzes a related reaction.
Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Putative cysteine synthase		PRO_0000167136
Regions
	Region	179 – 183	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	71	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	41	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55708-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9D31197DE4163A66	FASTA	336	35,415
	10 20 30 40 50 60 MLHTTVTQLI GQTPVMSIDV PGRNATLVLK IEKNNPGGSM KDRMARSMVI AALQDGRLPP 70 80 90 100 110 120 GGTIVESSSG NTGTGLALAA LEFGLRFIAV VDHHAAPDKI RMMRALGAEI RYVEGDFRED 130 140 150 160 170 180 EVAVVERQRL AAQLGAQLPG ALFMNQSDNP ANPEGYTGLV DELVAQLPDG IDAFVGCVGT 190 200 210 220 230 240 GGSMTGISQR LKRNNPAVRT IAVEPAGSIV FGKPGHPYYQ SGTGTPAGDE VGKVLDYGCI 250 260 270 280 290 300 DEGVQVTDTQ AFETARYIAR RKGLLVGGST GGAIYKALEF IGAGKLTGTV VTTVADGGEK 310 320 330 YLGTIFDEEW MAKRRLLDPA IAAQLDGWLF GKARAA

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00090 Genomic DNA. Translation: AAB91939.1.
RefSeq	NP_444152.1.
3D structure databases
HSSP	HSSP built from PDB template 1JBQ based on UniProtKB P35520.
ModBase	Search...
Genome annotation databases
GeneID	962517.
GenomeReviews	Gene locus NGR_a00730 in contig U00090_GR.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y4XP_RHISN

Accession

Primary (citable) accession number: P55708

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 22, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)