P55704 (NOPL_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nodulation outer protein L

Gene names

Name:	nopL
Ordered Locus Names:	NGR_a00770
ORF Names:	y4xL

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative symbiotic effector that modulates nodulation in legumes. When delivered into the plant cell, modulates the activity of signal transduction pathways that culminate in activation of PR proteins. Ref.4 Ref.5
Subcellular location	Secreted. Note: Secreted by the type II secretion system and delivered into the host plant cytoplasm. Ref.3

Ontologies

Keywords
Biological process	Nodulation
Cellular component	Secreted
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	nodulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Nodulation outer protein L		PRO_0000200967

Sequences

	Sequence		Length	Mass (Da)
	P55704 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8AD9AE9CFDF5E5AE	FASTA	338	37,145
	10 20 30 40 50 60 MDINSTSPLN ASPQPDSPPP ANASAFAHQL SGFQYSPPHA ADSLLPQVEA DSPYLDTRHP 70 80 90 100 110 120 YSQYLDSAYP YPSPCEWQHD LYTRTRERSP HPSEQRPHAR VLQGAPEHDQ DQHLEAAGPR 130 140 150 160 170 180 EGSWQVGPSR SGPSQAGLSP SATPLNPSPP PHATDLETKH PYSQYLDWAN PSLLDWQQDL 190 200 210 220 230 240 HTRATASPAP LTAERGRSPQ PSEQQPHARA LQVPEYDQDL IWQRVDAAGP QAGPWQVGPS 250 260 270 280 290 300 HSGPSQARPS HAWPSSSAGA EPAELSDFVM DSGVRAWDHW FLAPHMASED QMSMLRATGL 310 320 330 MPTAEVPTTT FLMMGMRHVA EFRGEGVIRI RPSVDFDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[2]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[3]	"Symbiotic implications of type III protein secretion machinery in Rhizobium." Viprey V., Del Greco A., Golinowski W., Broughton W.J., Perret X. Mol. Microbiol. 28:1381-1389(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-8, SUBCELLULAR LOCATION.
[4]	"Purification and phosphorylation of the effector protein NopL from Rhizobium sp. NGR234." Bartsev A.V., Boukli N.M., Deakin W.J., Staehelin C., Broughton W.J. FEBS Lett. 554:271-274(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"NopL, an effector protein of Rhizobium sp. NGR234, thwarts activation of plant defense reactions." Bartsev A.V., Deakin W.J., Boukli N.M., McAlvin C.B., Stacey G., Malnoe P., Broughton W.J., Staehelin C. Plant Physiol. 134:871-879(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91935.1.
RefSeq	NP_444148.1. NC_000914.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a00770.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91935; AAB91935; NGR_a00770.
GeneID	962508.
KEGG	rhi:NGR_a00770.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG12793.
ProtClustDB	CLSK516134.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

NOPL_RHISN

Accession

Primary (citable) accession number: P55704

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program