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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10D-glucose 6-phosphateBy similarity1
Binding sitei81D-glucose 6-phosphateBy similarity1
Sitei90Involved in alpha anomer selectivityBy similarity1
Binding sitei135D-glucose 6-phosphateBy similarity1
Sitei160Involved in alpha anomer selectivityBy similarity1
Binding sitei268UDP-glucoseBy similarity1
Binding sitei273UDP-glucoseBy similarity1
Binding sitei306D-glucose 6-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
UDP-glucose-glucosephosphate glucosyltransferaseBy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:NGR_a02090
ORF Names:y4pC
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224911 – 464Trehalose-6-phosphate synthaseAdd BLAST464

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP55612.
SMRiP55612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 24UDP-glucose bindingBy similarity2
Regioni371 – 375UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiPOG093E054C.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

P55612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVIVSNR VPVPDKGGIA PAGGLAVALK VALEEQGGGI WMGWSGKSSG
60 70 80 90 100
EDEPAPLAQL QQGNITYALT DLTDTDVEEY YHGFANRVLW PICHYRLDLA
110 120 130 140 150
EYGRKEMAGY FRVNRFFAHR LAPLVKPDDV IWVHDYPLIP LAAELRQMGL
160 170 180 190 200
ENRIGFFLHI PWPPADVLFT MPVHEEIMRG LSHYDVVGFQ TDHDLENFAS
210 220 230 240 250
CLRREGIGDA LGGGRLSAYG RIFKGGVYAI GIETAAFAEF AKKASTNSTV
260 270 280 290 300
KKARESIERR SLIIGVDRLD YSKGLTQRIE AFERFILANP AQRGRVTYLQ
310 320 330 340 350
ITPKSRSEVP EYEAMQRTVA EQAGRVNGAL GAVDWVPMRY INRSVGRRVL
360 370 380 390 400
AGLYRLGKVG LVTPLRDGKN LVAKEYVAAQ DPDDPGVLVL SRFAGAAREL
410 420 430 440 450
QGALLVNPYD IEGTANAMAR SLSMPLEERQ ERWTTMMDQL LEHDVSRWCR
460
DFLNDLTASS DRCG
Length:464
Mass (Da):51,627
Last modified:November 1, 1997 - v1
Checksum:i96DC610DD739FA73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91813.1.
RefSeqiNP_444016.1. NC_000914.2.
WP_010875236.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91813; AAB91813; NGR_a02090.
GeneIDi962413.
KEGGirhi:NGR_a02090.
PATRICi32301169. VBIRhiSp122450_0220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91813.1.
RefSeqiNP_444016.1. NC_000914.2.
WP_010875236.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55612.
SMRiP55612.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91813; AAB91813; NGR_a02090.
GeneIDi962413.
KEGGirhi:NGR_a02090.
PATRICi32301169. VBIRhiSp122450_0220.

Phylogenomic databases

HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiPOG093E054C.

Enzyme and pathway databases

UniPathwayiUPA00299.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOTSA_SINFN
AccessioniPrimary (citable) accession number: P55612
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 15, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.