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Protein

Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei81 – 811Glucose-6-phosphateBy similarity
Sitei90 – 901Involved in alpha anomer selectivityBy similarity
Binding sitei135 – 1351Glucose-6-phosphateBy similarity
Sitei160 – 1601Involved in alpha anomer selectivityBy similarity
Binding sitei268 – 2681UDP-glucoseBy similarity
Binding sitei273 – 2731UDP-glucoseBy similarity
Binding sitei306 – 3061Glucose-6-phosphateBy similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6260-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:NGR_a02090
ORF Names:y4pC
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000122491Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi394.NGR_a02090.

Structurei

3D structure databases

ProteinModelPortaliP55612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 242UDP-glucose bindingBy similarity
Regioni371 – 3755UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

P55612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVIVSNR VPVPDKGGIA PAGGLAVALK VALEEQGGGI WMGWSGKSSG
60 70 80 90 100
EDEPAPLAQL QQGNITYALT DLTDTDVEEY YHGFANRVLW PICHYRLDLA
110 120 130 140 150
EYGRKEMAGY FRVNRFFAHR LAPLVKPDDV IWVHDYPLIP LAAELRQMGL
160 170 180 190 200
ENRIGFFLHI PWPPADVLFT MPVHEEIMRG LSHYDVVGFQ TDHDLENFAS
210 220 230 240 250
CLRREGIGDA LGGGRLSAYG RIFKGGVYAI GIETAAFAEF AKKASTNSTV
260 270 280 290 300
KKARESIERR SLIIGVDRLD YSKGLTQRIE AFERFILANP AQRGRVTYLQ
310 320 330 340 350
ITPKSRSEVP EYEAMQRTVA EQAGRVNGAL GAVDWVPMRY INRSVGRRVL
360 370 380 390 400
AGLYRLGKVG LVTPLRDGKN LVAKEYVAAQ DPDDPGVLVL SRFAGAAREL
410 420 430 440 450
QGALLVNPYD IEGTANAMAR SLSMPLEERQ ERWTTMMDQL LEHDVSRWCR
460
DFLNDLTASS DRCG
Length:464
Mass (Da):51,627
Last modified:November 1, 1997 - v1
Checksum:i96DC610DD739FA73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91813.1.
RefSeqiNP_444016.1. NC_000914.2.
WP_010875236.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91813; AAB91813; NGR_a02090.
GeneIDi962413.
KEGGirhi:NGR_a02090.
PATRICi32301169. VBIRhiSp122450_0220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91813.1.
RefSeqiNP_444016.1. NC_000914.2.
WP_010875236.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55612.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_a02090.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91813; AAB91813; NGR_a02090.
GeneIDi962413.
KEGGirhi:NGR_a02090.
PATRICi32301169. VBIRhiSp122450_0220.

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Enzyme and pathway databases

UniPathwayiUPA00299.
BioCyciSFRE394:GBYN-6260-MONOMER.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiOTSA_RHISN
AccessioniPrimary (citable) accession number: P55612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 27, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.