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P55612

- OTSA_RHISN

UniProt

P55612 - OTSA_RHISN

Protein

Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate.By similarity

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Glucose-6-phosphateBy similarity
    Binding sitei81 – 811Glucose-6-phosphateBy similarity
    Sitei90 – 901Involved in alpha anomer selectivityBy similarity
    Binding sitei135 – 1351Glucose-6-phosphateBy similarity
    Sitei160 – 1601Involved in alpha anomer selectivityBy similarity
    Binding sitei268 – 2681UDP-glucoseBy similarity
    Binding sitei273 – 2731UDP-glucoseBy similarity
    Binding sitei306 – 3061Glucose-6-phosphateBy similarity
    Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSFRE394:GBYN-6260-MONOMER.
    UniPathwayiUPA00299.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
    Alternative name(s):
    Osmoregulatory trehalose synthesis protein A
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:otsA
    Ordered Locus Names:NGR_a02090
    ORF Names:y4pC
    Encoded oniPlasmid sym pNGR234a0 Publication
    OrganismiRhizobium sp. (strain NGR234)
    Taxonomic identifieri394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001054: Plasmid sym pNGR234a

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000122491Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi394.NGR_a02090.

    Structurei

    3D structure databases

    ProteinModelPortaliP55612.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni23 – 242UDP-glucose bindingBy similarity
    Regioni371 – 3755UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191478.
    KOiK00697.
    OMAiWGESFVK.
    OrthoDBiEOG6CCH1M.

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P55612-1 [UniParc]FASTAAdd to Basket

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    MSRLVIVSNR VPVPDKGGIA PAGGLAVALK VALEEQGGGI WMGWSGKSSG    50
    EDEPAPLAQL QQGNITYALT DLTDTDVEEY YHGFANRVLW PICHYRLDLA 100
    EYGRKEMAGY FRVNRFFAHR LAPLVKPDDV IWVHDYPLIP LAAELRQMGL 150
    ENRIGFFLHI PWPPADVLFT MPVHEEIMRG LSHYDVVGFQ TDHDLENFAS 200
    CLRREGIGDA LGGGRLSAYG RIFKGGVYAI GIETAAFAEF AKKASTNSTV 250
    KKARESIERR SLIIGVDRLD YSKGLTQRIE AFERFILANP AQRGRVTYLQ 300
    ITPKSRSEVP EYEAMQRTVA EQAGRVNGAL GAVDWVPMRY INRSVGRRVL 350
    AGLYRLGKVG LVTPLRDGKN LVAKEYVAAQ DPDDPGVLVL SRFAGAAREL 400
    QGALLVNPYD IEGTANAMAR SLSMPLEERQ ERWTTMMDQL LEHDVSRWCR 450
    DFLNDLTASS DRCG 464
    Length:464
    Mass (Da):51,627
    Last modified:November 1, 1997 - v1
    Checksum:i96DC610DD739FA73
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00090 Genomic DNA. Translation: AAB91813.1.
    RefSeqiNP_444016.1. NC_000914.2.

    Genome annotation databases

    EnsemblBacteriaiAAB91813; AAB91813; NGR_a02090.
    GeneIDi962413.
    KEGGirhi:NGR_a02090.
    PATRICi32301169. VBIRhiSp122450_0220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00090 Genomic DNA. Translation: AAB91813.1 .
    RefSeqi NP_444016.1. NC_000914.2.

    3D structure databases

    ProteinModelPortali P55612.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394.NGR_a02090.

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB91813 ; AAB91813 ; NGR_a02090 .
    GeneIDi 962413.
    KEGGi rhi:NGR_a02090.
    PATRICi 32301169. VBIRhiSp122450_0220.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191478.
    KOi K00697.
    OMAi WGESFVK.
    OrthoDBi EOG6CCH1M.

    Enzyme and pathway databases

    UniPathwayi UPA00299 .
    BioCyci SFRE394:GBYN-6260-MONOMER.

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular basis of symbiosis between Rhizobium and legumes."
      Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
      Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NGR234.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NGR234.

    Entry informationi

    Entry nameiOTSA_RHISN
    AccessioniPrimary (citable) accession number: P55612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Rhizobium plasmid pNGR234a
      Rhizobium plasmid pNGR234a: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3