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P55612 (OTSA_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]

EC=2.4.1.15
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:otsA
Ordered Locus Names:NGR_a02090
ORF Names:y4pC
Encoded onPlasmid sym pNGR234a
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate By similarity.

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 464464Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000122491

Regions

Region23 – 242UDP-glucose binding By similarity
Region371 – 3755UDP-glucose binding By similarity

Sites

Binding site101Glucose-6-phosphate By similarity
Binding site811Glucose-6-phosphate By similarity
Binding site1351Glucose-6-phosphate By similarity
Binding site2681UDP-glucose By similarity
Binding site2731UDP-glucose By similarity
Binding site3061Glucose-6-phosphate By similarity
Binding site3401UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
Site901Involved in alpha anomer selectivity By similarity
Site1601Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
P55612 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 96DC610DD739FA73

FASTA46451,627
        10         20         30         40         50         60 
MSRLVIVSNR VPVPDKGGIA PAGGLAVALK VALEEQGGGI WMGWSGKSSG EDEPAPLAQL 

        70         80         90        100        110        120 
QQGNITYALT DLTDTDVEEY YHGFANRVLW PICHYRLDLA EYGRKEMAGY FRVNRFFAHR 

       130        140        150        160        170        180 
LAPLVKPDDV IWVHDYPLIP LAAELRQMGL ENRIGFFLHI PWPPADVLFT MPVHEEIMRG 

       190        200        210        220        230        240 
LSHYDVVGFQ TDHDLENFAS CLRREGIGDA LGGGRLSAYG RIFKGGVYAI GIETAAFAEF 

       250        260        270        280        290        300 
AKKASTNSTV KKARESIERR SLIIGVDRLD YSKGLTQRIE AFERFILANP AQRGRVTYLQ 

       310        320        330        340        350        360 
ITPKSRSEVP EYEAMQRTVA EQAGRVNGAL GAVDWVPMRY INRSVGRRVL AGLYRLGKVG 

       370        380        390        400        410        420 
LVTPLRDGKN LVAKEYVAAQ DPDDPGVLVL SRFAGAAREL QGALLVNPYD IEGTANAMAR 

       430        440        450        460 
SLSMPLEERQ ERWTTMMDQL LEHDVSRWCR DFLNDLTASS DRCG 

« Hide

References

[1]"Molecular basis of symbiosis between Rhizobium and legumes."
Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[2]"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems."
Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.
Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00090 Genomic DNA. Translation: AAB91813.1.
RefSeqNP_444016.1. NC_000914.2.

3D structure databases

ProteinModelPortalP55612.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_a02090.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91813; AAB91813; NGR_a02090.
GeneID962413.
KEGGrhi:NGR_a02090.
PATRIC32301169. VBIRhiSp122450_0220.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0380.
HOGENOMHOG000191478.
KOK00697.
OMAWGESFVK.
OrthoDBEOG6CCH1M.

Enzyme and pathway databases

BioCycSFRE394:GBYN-6260-MONOMER.
UniPathwayUPA00299.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameOTSA_RHISN
AccessionPrimary (citable) accession number: P55612
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names