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P55612

- OTSA_RHISN

UniProt

P55612 - OTSA_RHISN

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Protein
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]
Gene
otsA, NGR_a02090, y4pC
Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate By similarity.

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphate By similarity
Binding sitei81 – 811Glucose-6-phosphate By similarity
Sitei90 – 901Involved in alpha anomer selectivity By similarity
Binding sitei135 – 1351Glucose-6-phosphate By similarity
Sitei160 – 1601Involved in alpha anomer selectivity By similarity
Binding sitei268 – 2681UDP-glucose By similarity
Binding sitei273 – 2731UDP-glucose By similarity
Binding sitei306 – 3061Glucose-6-phosphate By similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. trehalose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6260-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:NGR_a02090
ORF Names:y4pC
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000122491Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi394.NGR_a02090.

Structurei

3D structure databases

ProteinModelPortaliP55612.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 242UDP-glucose binding By similarity
Regioni371 – 3755UDP-glucose binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiWGESFVK.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

P55612-1 [UniParc]FASTAAdd to Basket

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MSRLVIVSNR VPVPDKGGIA PAGGLAVALK VALEEQGGGI WMGWSGKSSG    50
EDEPAPLAQL QQGNITYALT DLTDTDVEEY YHGFANRVLW PICHYRLDLA 100
EYGRKEMAGY FRVNRFFAHR LAPLVKPDDV IWVHDYPLIP LAAELRQMGL 150
ENRIGFFLHI PWPPADVLFT MPVHEEIMRG LSHYDVVGFQ TDHDLENFAS 200
CLRREGIGDA LGGGRLSAYG RIFKGGVYAI GIETAAFAEF AKKASTNSTV 250
KKARESIERR SLIIGVDRLD YSKGLTQRIE AFERFILANP AQRGRVTYLQ 300
ITPKSRSEVP EYEAMQRTVA EQAGRVNGAL GAVDWVPMRY INRSVGRRVL 350
AGLYRLGKVG LVTPLRDGKN LVAKEYVAAQ DPDDPGVLVL SRFAGAAREL 400
QGALLVNPYD IEGTANAMAR SLSMPLEERQ ERWTTMMDQL LEHDVSRWCR 450
DFLNDLTASS DRCG 464
Length:464
Mass (Da):51,627
Last modified:November 1, 1997 - v1
Checksum:i96DC610DD739FA73
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00090 Genomic DNA. Translation: AAB91813.1.
RefSeqiNP_444016.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91813; AAB91813; NGR_a02090.
GeneIDi962413.
KEGGirhi:NGR_a02090.
PATRICi32301169. VBIRhiSp122450_0220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00090 Genomic DNA. Translation: AAB91813.1 .
RefSeqi NP_444016.1. NC_000914.2.

3D structure databases

ProteinModelPortali P55612.
ModBasei Search...

Protein-protein interaction databases

STRINGi 394.NGR_a02090.

Protein family/group databases

CAZyi GT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB91813 ; AAB91813 ; NGR_a02090 .
GeneIDi 962413.
KEGGi rhi:NGR_a02090.
PATRICi 32301169. VBIRhiSp122450_0220.

Phylogenomic databases

eggNOGi COG0380.
HOGENOMi HOG000191478.
KOi K00697.
OMAi WGESFVK.
OrthoDBi EOG6CCH1M.

Enzyme and pathway databases

UniPathwayi UPA00299 .
BioCyci SFRE394:GBYN-6260-MONOMER.

Family and domain databases

InterProi IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view ]
Pfami PF00982. Glyco_transf_20. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiOTSA_RHISN
AccessioniPrimary (citable) accession number: P55612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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