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P55475

- NODJ_RHISN

UniProt

P55475 - NODJ_RHISN

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Protein

Nodulation protein J

Gene

nodJ

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Part of the ABC transporter complex NodIJ involved in the export of the nodulation factors (Nod factors), the bacterial signal molecules that induce symbiosis and subsequent nodulation induction. Nod factors are LCO (lipo-chitin oligosaccharide), a modified beta-1,4-linked N-acetylglucosamine oligosaccharide. This subunit encodes the transporter (By similarity).By similarity

GO - Molecular functioni

  1. ATPase activity, coupled to transmembrane movement of substances Source: InterPro
  2. ATP binding Source: InterPro

GO - Biological processi

  1. nodulation Source: UniProtKB-KW
  2. oligosaccharide transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Nodulation, Transport

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6396-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nodulation protein J
Gene namesi
Name:nodJ
Ordered Locus Names:NGR_a03450
ORF Names:y4hE
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054: Plasmid sym pNGR234a

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei35 – 5521HelicalSequence AnalysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence AnalysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence AnalysisAdd
BLAST
Transmembranei236 – 25621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Nodulation protein JPRO_0000182991Add
BLAST

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (NodI) and two transmembrane proteins (NodJ).Curated

Protein-protein interaction databases

STRINGi394.NGR_a03450.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 259227ABC transmembrane type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0842.
HOGENOMiHOG000225586.
KOiK09694.
OMAiLPANAWN.
OrthoDBiEOG6B627P.

Family and domain databases

InterProiIPR013525. ABC_2_trans.
IPR000412. ABC_2_transport.
IPR005981. ABC_transptNodJ.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
[Graphical view]
PIRSFiPIRSF006648. DrrB. 1 hit.
PRINTSiPR00164. ABC2TRNSPORT.
TIGRFAMsiTIGR01291. nodJ. 1 hit.
PROSITEiPS51012. ABC_TM2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55475-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWKRYAAVLP ANPWNWIAVW RRNYMAWKKA AIASILGNLA EPVTSLFGLG
60 70 80 90 100
FGLGAMVGRV DGIPYVAFLA AGMVATSAMI SATFETIHAT FARMQAKRTW
110 120 130 140 150
ESALCTQLTL GDIVLGELAW AASKALLAGT AMMLVAATMG FASWPSVLFA
160 170 180 190 200
LPVIALTGFA FASLAMIVTA LAPGYDYFIF YQTLFLTPML FLSGAVFPVS
210 220 230 240 250
QLPDIFQKLS HLLPLAHSIE LIRPAMLDRP GGGVALHISA LCIFAVMPFF
260
LSVGLLQRRL LS
Length:262
Mass (Da):28,294
Last modified:November 1, 1997 - v1
Checksum:iEC1DF7B2FE7852F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91693.1.
RefSeqiNP_443881.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91693; AAB91693; NGR_a03450.
GeneIDi962495.
KEGGirhi:NGR_a03450.
PATRICi32301437. VBIRhiSp122450_0354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91693.1 .
RefSeqi NP_443881.1. NC_000914.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 394.NGR_a03450.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB91693 ; AAB91693 ; NGR_a03450 .
GeneIDi 962495.
KEGGi rhi:NGR_a03450.
PATRICi 32301437. VBIRhiSp122450_0354.

Phylogenomic databases

eggNOGi COG0842.
HOGENOMi HOG000225586.
KOi K09694.
OMAi LPANAWN.
OrthoDBi EOG6B627P.

Enzyme and pathway databases

BioCyci SFRE394:GBYN-6396-MONOMER.

Family and domain databases

InterProi IPR013525. ABC_2_trans.
IPR000412. ABC_2_transport.
IPR005981. ABC_transptNodJ.
[Graphical view ]
Pfami PF01061. ABC2_membrane. 1 hit.
[Graphical view ]
PIRSFi PIRSF006648. DrrB. 1 hit.
PRINTSi PR00164. ABC2TRNSPORT.
TIGRFAMsi TIGR01291. nodJ. 1 hit.
PROSITEi PS51012. ABC_TM2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiNODJ_RHISN
AccessioniPrimary (citable) accession number: P55475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3