P55475 (NODJ_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nodulation protein J

Gene names

Name:	nodJ
Ordered Locus Names:	NGR_a03450
ORF Names:	y4hE

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex NodIJ involved in the export of the nodulation factors (Nod factors), the bacterial signal molecules that induce symbiosis and subsequent nodulation induction. Nod factors are LCO (lipo-chitin oligosaccharide), a modified beta-1,4-linked N-acetylglucosamine oligosaccharide. This subunit encodes the transporter By similarity.
Subunit structure	The complex is composed of two ATP-binding proteins (NodI) and two transmembrane proteins (NodJ) Probable.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ABC-2 integral membrane protein family. Lipooligosaccharide exporter (TC 3.A.1.102) subfamily. [View classification] Contains 1 ABC transmembrane type-2 domain.

Ontologies

Keywords
Biological process	Nodulation Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	nodulation Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharide transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Nodulation protein J		PRO_0000182991
Regions
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Transmembrane	177 – 197	21	Helical; Potential
	Transmembrane	236 – 256	21	Helical; Potential
	Domain	33 – 259	227	ABC transmembrane type-2

Sequences

	Sequence		Length	Mass (Da)
	P55475 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: EC1DF7B2FE7852F0	FASTA	262	28,294
	10 20 30 40 50 60 MWKRYAAVLP ANPWNWIAVW RRNYMAWKKA AIASILGNLA EPVTSLFGLG FGLGAMVGRV 70 80 90 100 110 120 DGIPYVAFLA AGMVATSAMI SATFETIHAT FARMQAKRTW ESALCTQLTL GDIVLGELAW 130 140 150 160 170 180 AASKALLAGT AMMLVAATMG FASWPSVLFA LPVIALTGFA FASLAMIVTA LAPGYDYFIF 190 200 210 220 230 240 YQTLFLTPML FLSGAVFPVS QLPDIFQKLS HLLPLAHSIE LIRPAMLDRP GGGVALHISA 250 260 LCIFAVMPFF LSVGLLQRRL LS

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91693.1.
RefSeq	NP_443881.1. NC_000914.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a03450.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91693; AAB91693; NGR_a03450.
GeneID	962495.
KEGG	rhi:NGR_a03450.
PATRIC	32301437. VBIRhiSp122450_0354.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0842.
HOGENOM	HOG000225586.
KO	K09694.
OMA	LAWAATK.
Family and domain databases
InterPro	IPR000412. ABC_2. IPR013526. ABC_2_tran. IPR013525. ABC_2_trans. IPR005981. ABC_transptNodJ. [Graphical view]
Pfam	PF01061. ABC2_membrane. 1 hit. [Graphical view]
PRINTS	PR00164. ABC2TRNSPORT.
TIGRFAMs	TIGR01291. nodJ. 1 hit.
PROSITE	PS51012. ABC_TM2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NODJ_RHISN

Accession

Primary (citable) accession number: P55475

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program