ID   NOEI_SINFN              Reviewed;         243 AA.
AC   P55473;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 110.
DE   RecName: Full=2-O-methyltransferase NoeI;
DE            EC=2.1.1.-;
DE   AltName: Full=Nodulation protein NoeI;
GN   Name=noeI; OrderedLocusNames=NGR_a03470; ORFNames=y4hC;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
RN   [3]
RP   FUNCTION IN METHYLATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9575146; DOI=10.1074/jbc.273.20.12047;
RA   Jabbouri S., Relic B., Hanin M., Kamalaprija P., Burger U., Prome D.,
RA   Prome J.C., Broughton W.J.;
RT   "nolO and noeI (HsnIII) of Rhizobium sp. NGR234 are involved in 3-O-
RT   carbamoylation and 2-O-methylation of Nod factors.";
RL   J. Biol. Chem. 273:12047-12055(1998).
CC   -!- FUNCTION: Required for 2-O-methylation of the fucosyl group of Nod
CC       factors. {ECO:0000269|PubMed:9575146}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Disruption of the gene has no effect on
CC       nodulation. {ECO:0000269|PubMed:9575146}.
CC   -!- SIMILARITY: Belongs to the FkbM methyltransferase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00090; AAB91691.1; -; Genomic_DNA.
DR   RefSeq; NP_443879.1; NC_000914.2.
DR   RefSeq; WP_010875361.1; NC_000914.2.
DR   AlphaFoldDB; P55473; -.
DR   SMR; P55473; -.
DR   KEGG; rhi:NGR_a03470; -.
DR   eggNOG; COG0275; Bacteria.
DR   HOGENOM; CLU_068034_3_2_5; -.
DR   OrthoDB; 9814604at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR006342; FkbM_mtfrase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; TIGR01444; fkbM_fam; 1.
DR   PANTHER; PTHR36973:SF4; NODULATION PROTEIN; 1.
DR   PANTHER; PTHR36973; SLL1456 PROTEIN-RELATED; 1.
DR   Pfam; PF05050; Methyltransf_21; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Methyltransferase; Nodulation; Plasmid; Reference proteome;
KW   Transferase.
FT   CHAIN           1..243
FT                   /note="2-O-methyltransferase NoeI"
FT                   /id="PRO_0000096924"
SQ   SEQUENCE   243 AA;  27735 MW;  D22A60D7AAAB87C1 CRC64;
     MEVGRYLKEG LLISLQQRLR EFAEGIRKPT SSLKVHRQIV SLLEKPDPVI LDIGCNDGSD
     ARRFLQLRPK AQLFCFEPDP RAAARCRENM GPLDRMRLFE VAISDRNGRI DFHPSNGDGD
     AKEWDLSGSI RQPKNHLSEY QWVRFDGPIS VETRRLDDWC SEAGLESIDL IWMDVQGAES
     DVIAGGKETL TKTRFIYTEY SDQELYEGQL PLRAILDLLP SFELVAQFPR GVEGDVLLRN
     TKL
//