P55473 (NOEI_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-O-methyltransferase NoeI
EC=2.1.1.-

Alternative name(s):
Nodulation protein NoeI

Gene names

Name:	noeI
Ordered Locus Names:	NGR_a03470
ORF Names:	y4hC

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for 2-O-methylation of the fucosyl group of Nod factors. Ref.3
Subcellular location	Cytoplasm Potential.
Disruption phenotype	Disruption of the gene has no effect on nodulation. Ref.3
Sequence similarities	Belongs to the FkbM methyltransferase family.

Ontologies

Keywords
Biological process	Nodulation
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	nodulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	2-O-methyltransferase NoeI		PRO_0000096924

Sequences

	Sequence		Length	Mass (Da)
	P55473 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D22A60D7AAAB87C1	FASTA	243	27,735
	10 20 30 40 50 60 MEVGRYLKEG LLISLQQRLR EFAEGIRKPT SSLKVHRQIV SLLEKPDPVI LDIGCNDGSD 70 80 90 100 110 120 ARRFLQLRPK AQLFCFEPDP RAAARCRENM GPLDRMRLFE VAISDRNGRI DFHPSNGDGD 130 140 150 160 170 180 AKEWDLSGSI RQPKNHLSEY QWVRFDGPIS VETRRLDDWC SEAGLESIDL IWMDVQGAES 190 200 210 220 230 240 DVIAGGKETL TKTRFIYTEY SDQELYEGQL PLRAILDLLP SFELVAQFPR GVEGDVLLRN TKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[2]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[3]	"nolO and noeI (HsnIII) of Rhizobium sp. NGR234 are involved in 3-O-carbamoylation and 2-O-methylation of Nod factors." Jabbouri S., Relic B., Hanin M., Kamalaprija P., Burger U., Prome D., Prome J.C., Broughton W.J. J. Biol. Chem. 273:12047-12055(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN METHYLATION, DISRUPTION PHENOTYPE. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91691.1.
RefSeq	NP_443879.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55473.
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a03470.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91691; AAB91691; NGR_a03470.
GeneID	962315.
KEGG	rhi:NGR_a03470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HOG000066707.
OMA	ELYEGQL.
ProtClustDB	CLSK516088.
Enzyme and pathway databases
BioCyc	SFRE394:GBYN-6398-MONOMER.
Family and domain databases
InterPro	IPR006342. FkbM_mtfrase. [Graphical view]
Pfam	PF05050. Methyltransf_21. 1 hit. [Graphical view]
TIGRFAMs	TIGR01444. fkbM_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NOEI_RHISN

Accession

Primary (citable) accession number: P55473

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program