P55464 (RMLA_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glucose-1-phosphate thymidylyltransferase
EC=2.7.7.24

Alternative name(s):
dTDP-glucose pyrophosphorylase
dTDP-glucose synthase

Gene names

Name:	rmlA
Ordered Locus Names:	NGR_a03560
ORF Names:	y4gH

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis By similarity.
Catalytic activity	dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glucose-1-phosphate thymidylyltransferase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	extracellular polysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glucose-1-phosphate thymidylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Probable glucose-1-phosphate thymidylyltransferase		PRO_0000208003
Sites
	Metal binding	108	1	Magnesium By similarity
	Metal binding	223	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55464 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2FD7BFF7575914F2	FASTA	286	31,205
	10 20 30 40 50 60 MKGIILAGGS GTRLHPMTLV MSKQILPVYD KPMIFYPLTT LMLAGIREIL IISTPHHMPL 70 80 90 100 110 120 FQALLGDGSQ WGISLRYAVQ PSPNGLAQAY VIGADFVAGS PSCLILGDNI YFGHGLQGLL 130 140 150 160 170 180 QQAAALQQGA TIFAYHVNDP ERYGVVEFDE GMNALSIEEK PAAPKSTWAV TGLYFYDSEV 190 200 210 220 230 240 VDIAANLKPS ARGEYEITDV NRIYLERGKL KVAVLGRGYA WLDTGTPDSL LEAAEFVRTL 250 260 270 280 EKRQAFKVAC PEEVALAMGF ISVEEFARIA ERAGKGDYGA YLRRLA

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91682.1.
RefSeq	NP_443870.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55464.
SMR	P55464. Positions 2-285.
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a03560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91682; AAB91682; NGR_a03560.
GeneID	962578.
KEGG	rhi:NGR_a03560.
PATRIC	32301457. VBIRhiSp122450_0364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1209.
HOGENOM	HOG000283473.
KO	K00973.
OMA	GHYLLRL.
ProtClustDB	CLSK2518392.
Family and domain databases
InterPro	IPR005907. G1P_thy_trans_s. IPR005835. NTP_transferase. [Graphical view]
PANTHER	PTHR22572:SF13. PTHR22572:SF13. 1 hit.
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01207. rmlA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RMLA_RHISN

Accession

Primary (citable) accession number: P55464

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program