Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable glucose-1-phosphate thymidylyltransferase

Gene

rmlA

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.By similarity

Catalytic activityi

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081MagnesiumBy similarity
Metal bindingi223 – 2231MagnesiumBy similarity

GO - Molecular functioni

  1. glucose-1-phosphate thymidylyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. extracellular polysaccharide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-760-MONOMER.
SFRE394:GBYN-6407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glucose-1-phosphate thymidylyltransferase (EC:2.7.7.24)
Alternative name(s):
dTDP-glucose pyrophosphorylase
dTDP-glucose synthase
Gene namesi
Name:rmlA
Ordered Locus Names:NGR_a03560
ORF Names:y4gH
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Probable glucose-1-phosphate thymidylyltransferasePRO_0000208003Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi394.NGR_a03560.

Structurei

3D structure databases

ProteinModelPortaliP55464.
SMRiP55464. Positions 2-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1209.
HOGENOMiHOG000283473.
KOiK00973.
OMAiYLVEVAN.
OrthoDBiEOG6RC3RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005907. G1P_thy_trans_s.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF13. PTHR22572:SF13. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01207. rmlA. 1 hit.

Sequencei

Sequence statusi: Complete.

P55464-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGIILAGGS GTRLHPMTLV MSKQILPVYD KPMIFYPLTT LMLAGIREIL
60 70 80 90 100
IISTPHHMPL FQALLGDGSQ WGISLRYAVQ PSPNGLAQAY VIGADFVAGS
110 120 130 140 150
PSCLILGDNI YFGHGLQGLL QQAAALQQGA TIFAYHVNDP ERYGVVEFDE
160 170 180 190 200
GMNALSIEEK PAAPKSTWAV TGLYFYDSEV VDIAANLKPS ARGEYEITDV
210 220 230 240 250
NRIYLERGKL KVAVLGRGYA WLDTGTPDSL LEAAEFVRTL EKRQAFKVAC
260 270 280
PEEVALAMGF ISVEEFARIA ERAGKGDYGA YLRRLA
Length:286
Mass (Da):31,205
Last modified:November 1, 1997 - v1
Checksum:i2FD7BFF7575914F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91682.1.
RefSeqiNP_443870.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91682; AAB91682; NGR_a03560.
GeneIDi962578.
KEGGirhi:NGR_a03560.
PATRICi32301457. VBIRhiSp122450_0364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91682.1.
RefSeqiNP_443870.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55464.
SMRiP55464. Positions 2-285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_a03560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91682; AAB91682; NGR_a03560.
GeneIDi962578.
KEGGirhi:NGR_a03560.
PATRICi32301457. VBIRhiSp122450_0364.

Phylogenomic databases

eggNOGiCOG1209.
HOGENOMiHOG000283473.
KOiK00973.
OMAiYLVEVAN.
OrthoDBiEOG6RC3RN.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-760-MONOMER.
SFRE394:GBYN-6407-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005907. G1P_thy_trans_s.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF13. PTHR22572:SF13. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01207. rmlA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiRMLA_RHISN
AccessioniPrimary (citable) accession number: P55464
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.