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Protein

Probable dTDP-4-dehydrorhamnose reductase

Gene

NGR_a03570

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well (By similarity).By similarity

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491Substrate; via amide nitrogenBy similarity
Binding sitei150 – 1501NAD; via amide nitrogenBy similarity
Binding sitei221 – 2211SubstrateBy similarity
Binding sitei256 – 2561SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 115NADBy similarity
Nucleotide bindingi11 – 122NADPBy similarity
Nucleotide bindingi30 – 312NADBy similarity
Nucleotide bindingi35 – 362NAD/NADPBy similarity
Nucleotide bindingi58 – 614NADBy similarity
Nucleotide bindingi59 – 613NADPBy similarity
Nucleotide bindingi124 – 1285NAD/NADPBy similarity

GO - Molecular functioni

  1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB

GO - Biological processi

  1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  2. extracellular polysaccharide biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6408-MONOMER.
UniPathwayiUPA00124.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Ordered Locus Names:NGR_a03570
ORF Names:y4gG
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Probable dTDP-4-dehydrorhamnose reductasePRO_0000207988Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi394.NGR_a03570.

Structurei

3D structure databases

ProteinModelPortaliP55463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni100 – 1012Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1091.
HOGENOMiHOG000227712.
KOiK00067.
OMAiIRTAWVY.
OrthoDBiEOG6HTP2V.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029900. RmlD.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PTHR10491:SF5. PTHR10491:SF5. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01214. rmlD. 1 hit.

Sequencei

Sequence statusi: Complete.

P55463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLAVTGKNG QIALALKAQA RPDVEILTLG RPNFDLACRS TVASSIRDAA
60 70 80 90 100
PDIIVSLAAY TAVDKAESEP YEAFAVNRDG VQALAEAAAG LGVPVIHLST
110 120 130 140 150
DYVFDGAKPV PYCEEDRTGP ISVYGRSKLE GEFAVASANP NHTILRTSWV
160 170 180 190 200
YSRYGQNFVK KMLRLADTND ELNVVADQLG CPTSADDISV AVMTIARRML
210 220 230 240 250
SSSSADLRGI FHLSGSGEAS WAAFAKYVFS VYDEITGRQI KVHDISAAEY
260 270 280 290
PTPARRPANS RLHCDKLERT FGIRLPNWEE STRRLVWALL LEGKDA
Length:296
Mass (Da):32,236
Last modified:November 1, 1997 - v1
Checksum:i36B97283479F2164
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91681.1.
RefSeqiNP_443869.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91681; AAB91681; NGR_a03570.
GeneIDi962163.
KEGGirhi:NGR_a03570.
PATRICi32301459. VBIRhiSp122450_0365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91681.1.
RefSeqiNP_443869.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_a03570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91681; AAB91681; NGR_a03570.
GeneIDi962163.
KEGGirhi:NGR_a03570.
PATRICi32301459. VBIRhiSp122450_0365.

Phylogenomic databases

eggNOGiCOG1091.
HOGENOMiHOG000227712.
KOiK00067.
OMAiIRTAWVY.
OrthoDBiEOG6HTP2V.

Enzyme and pathway databases

UniPathwayiUPA00124.
BioCyciSFRE394:GBYN-6408-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029900. RmlD.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PTHR10491:SF5. PTHR10491:SF5. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01214. rmlD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiRMLD_RHISN
AccessioniPrimary (citable) accession number: P55463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.