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Protein

Probable dTDP-4-dehydrorhamnose reductase

Gene

NGR_a03570

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well (By similarity).By similarity

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149Substrate; via amide nitrogenBy similarity1
Binding sitei150NAD; via amide nitrogenBy similarity1
Binding sitei221SubstrateBy similarity1
Binding sitei256SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 11NADBy similarity5
Nucleotide bindingi11 – 12NADPBy similarity2
Nucleotide bindingi30 – 31NADBy similarity2
Nucleotide bindingi35 – 36NAD/NADPBy similarity2
Nucleotide bindingi58 – 61NADBy similarity4
Nucleotide bindingi59 – 61NADPBy similarity3
Nucleotide bindingi124 – 128NAD/NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00124.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Ordered Locus Names:NGR_a03570
ORF Names:y4gG
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002079881 – 296Probable dTDP-4-dehydrorhamnose reductaseAdd BLAST296

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP55463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 101Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000227712.
KOiK00067.
OMAiGYASRYE.
OrthoDBiPOG091H07IL.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01214. rmlD. 1 hit.

Sequencei

Sequence statusi: Complete.

P55463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLAVTGKNG QIALALKAQA RPDVEILTLG RPNFDLACRS TVASSIRDAA
60 70 80 90 100
PDIIVSLAAY TAVDKAESEP YEAFAVNRDG VQALAEAAAG LGVPVIHLST
110 120 130 140 150
DYVFDGAKPV PYCEEDRTGP ISVYGRSKLE GEFAVASANP NHTILRTSWV
160 170 180 190 200
YSRYGQNFVK KMLRLADTND ELNVVADQLG CPTSADDISV AVMTIARRML
210 220 230 240 250
SSSSADLRGI FHLSGSGEAS WAAFAKYVFS VYDEITGRQI KVHDISAAEY
260 270 280 290
PTPARRPANS RLHCDKLERT FGIRLPNWEE STRRLVWALL LEGKDA
Length:296
Mass (Da):32,236
Last modified:November 1, 1997 - v1
Checksum:i36B97283479F2164
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91681.1.
RefSeqiNP_443869.1. NC_000914.2.
WP_010875371.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91681; AAB91681; NGR_a03570.
GeneIDi962163.
KEGGirhi:NGR_a03570.
PATRICi32301459. VBIRhiSp122450_0365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91681.1.
RefSeqiNP_443869.1. NC_000914.2.
WP_010875371.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55463.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91681; AAB91681; NGR_a03570.
GeneIDi962163.
KEGGirhi:NGR_a03570.
PATRICi32301459. VBIRhiSp122450_0365.

Phylogenomic databases

HOGENOMiHOG000227712.
KOiK00067.
OMAiGYASRYE.
OrthoDBiPOG091H07IL.

Enzyme and pathway databases

UniPathwayiUPA00124.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01214. rmlD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRMLD_SINFN
AccessioniPrimary (citable) accession number: P55463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.