P55463 (RMLD_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dTDP-4-dehydrorhamnose reductase
EC=1.1.1.133

Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase

Gene names

Ordered Locus Names:	NGR_a03570
ORF Names:	y4gG

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well By similarity.
Catalytic activity	dTDP-6-deoxy-beta-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-beta-L-mannose + NADPH.
Cofactor	Binds 1 magnesium ion per monomer By similarity.
Pathway	Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the dTDP-4-dehydrorhamnose reductase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	dTDP-rhamnose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway extracellular polysaccharide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	dTDP-4-dehydrorhamnose reductase activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Probable dTDP-4-dehydrorhamnose reductase		PRO_0000207988
Regions
	Nucleotide binding	7 – 11	5	NAD By similarity
	Nucleotide binding	11 – 12	2	NADP By similarity
	Nucleotide binding	30 – 31	2	NAD By similarity
	Nucleotide binding	35 – 36	2	NAD/NADP By similarity
	Nucleotide binding	58 – 61	4	NAD By similarity
	Nucleotide binding	59 – 61	3	NADP By similarity
	Nucleotide binding	124 – 128	5	NAD/NADP By similarity
	Region	100 – 101	2	Substrate binding By similarity
Sites
	Binding site	149	1	Substrate; via amide nitrogen By similarity
	Binding site	150	1	NAD; via amide nitrogen By similarity
	Binding site	221	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55463 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 36B97283479F2164	FASTA	296	32,236
	10 20 30 40 50 60 MRLAVTGKNG QIALALKAQA RPDVEILTLG RPNFDLACRS TVASSIRDAA PDIIVSLAAY 70 80 90 100 110 120 TAVDKAESEP YEAFAVNRDG VQALAEAAAG LGVPVIHLST DYVFDGAKPV PYCEEDRTGP 130 140 150 160 170 180 ISVYGRSKLE GEFAVASANP NHTILRTSWV YSRYGQNFVK KMLRLADTND ELNVVADQLG 190 200 210 220 230 240 CPTSADDISV AVMTIARRML SSSSADLRGI FHLSGSGEAS WAAFAKYVFS VYDEITGRQI 250 260 270 280 290 KVHDISAAEY PTPARRPANS RLHCDKLERT FGIRLPNWEE STRRLVWALL LEGKDA

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91681.1.
RefSeq	NP_443869.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55463.
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a03570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91681; AAB91681; NGR_a03570.
GeneID	962163.
KEGG	rhi:NGR_a03570.
PATRIC	32301459. VBIRhiSp122450_0365.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1091.
HOGENOM	HOG000227712.
KO	K00067.
OMA	SPTYTKD.
ProtClustDB	CLSK513391.
Enzyme and pathway databases
UniPathway	UPA00124.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR005913. dTDP_dehydrorham_reduct. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF04321. RmlD_sub_bind. 1 hit. [Graphical view]
TIGRFAMs	TIGR01214. rmlD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RMLD_RHISN

Accession

Primary (citable) accession number: P55463

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program