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P55463 (RMLD_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable dTDP-4-dehydrorhamnose reductase

EC=1.1.1.133
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene names
Ordered Locus Names:NGR_a03570
ORF Names:y4gG
Encoded onPlasmid sym pNGR234a
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well By similarity.

Catalytic activity

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactor

Binds 1 magnesium ion per monomer By similarity.

Pathway

Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the dTDP-4-dehydrorhamnose reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Probable dTDP-4-dehydrorhamnose reductase
PRO_0000207988

Regions

Nucleotide binding7 – 115NAD By similarity
Nucleotide binding11 – 122NADP By similarity
Nucleotide binding30 – 312NAD By similarity
Nucleotide binding35 – 362NAD/NADP By similarity
Nucleotide binding58 – 614NAD By similarity
Nucleotide binding59 – 613NADP By similarity
Nucleotide binding124 – 1285NAD/NADP By similarity
Region100 – 1012Substrate binding By similarity

Sites

Binding site1491Substrate; via amide nitrogen By similarity
Binding site1501NAD; via amide nitrogen By similarity
Binding site2211Substrate By similarity
Binding site2561Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P55463 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 36B97283479F2164

FASTA29632,236
        10         20         30         40         50         60 
MRLAVTGKNG QIALALKAQA RPDVEILTLG RPNFDLACRS TVASSIRDAA PDIIVSLAAY 

        70         80         90        100        110        120 
TAVDKAESEP YEAFAVNRDG VQALAEAAAG LGVPVIHLST DYVFDGAKPV PYCEEDRTGP 

       130        140        150        160        170        180 
ISVYGRSKLE GEFAVASANP NHTILRTSWV YSRYGQNFVK KMLRLADTND ELNVVADQLG 

       190        200        210        220        230        240 
CPTSADDISV AVMTIARRML SSSSADLRGI FHLSGSGEAS WAAFAKYVFS VYDEITGRQI 

       250        260        270        280        290 
KVHDISAAEY PTPARRPANS RLHCDKLERT FGIRLPNWEE STRRLVWALL LEGKDA 

« Hide

References

[1]"Molecular basis of symbiosis between Rhizobium and legumes."
Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[2]"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems."
Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.
Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00090 Genomic DNA. Translation: AAB91681.1.
RefSeqNP_443869.1. NC_000914.2.

3D structure databases

ProteinModelPortalP55463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_a03570.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91681; AAB91681; NGR_a03570.
GeneID962163.
KEGGrhi:NGR_a03570.
PATRIC32301459. VBIRhiSp122450_0365.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1091.
HOGENOMHOG000227712.
KOK00067.
OMAPYWRDSL.
OrthoDBEOG6HTP2V.
ProtClustDBCLSK513391.

Enzyme and pathway databases

BioCycSFRE394:GBYN-6408-MONOMER.
UniPathwayUPA00124.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
TIGRFAMsTIGR01214. rmlD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRMLD_RHISN
AccessionPrimary (citable) accession number: P55463
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: December 11, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names