P55421 (TRAG_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable conjugal transfer protein traG

Gene names

Name:	traG
Ordered Locus Names:	NGR_a03950
ORF Names:	y4dV

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the virD4/traG family.

Ontologies

Keywords
Biological process	Conjugation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	unidirectional conjugation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Probable conjugal transfer protein traG		PRO_0000221656
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Transmembrane	215 – 235	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P55421 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 12810EFE1CC8B874	FASTA	640	69,180
	10 20 30 40 50 60 MTVNRIALVV APVVLMVLTV IGMSGIEQCL STFGKTDAAR LALGRIGIAF PYVTAAAIAV 70 80 90 100 110 120 IFLFASAGSA IIKLAGWGAL AGSAATMLIA TMREASRLAA FAAQVPAGKS IASYLDHATL 130 140 150 160 170 180 IGATAALMSG CFSLRVALIG NAAFARAEPR RIRGKRALHG EADWMNMQDA ARLFSDAGGV 190 200 210 220 230 240 VIGERYRVDK DSTAARSFRA DDPETWGAGG KSPLLCFDGS FGSSHGIVFA GSGGFKTTSV 250 260 270 280 290 300 TIPTALKWGG ALVVLDPSNE VAPMVYEHRK AAARFIRILD PKEPETGFNA LDWIGRFGGT 310 320 330 340 350 360 KEEDIASVAS WIMSDSGGMR GVRDDFFRAS ALQLLTALIA DVCLSGHTDK ENQTLRQVRA 370 380 390 400 410 420 NLAEPEPKLR ERLQSIYDNS NSDFVKENVA AYVNMTPETF SGVYANAVKE THWLSYPNYA 430 440 450 460 470 480 ALVSGSTFST DALAAGETDV FINIDLKTLE THAGLARVII GSFLNAIYNR DGEVKGRALF 490 500 510 520 530 540 FLDEVARLGY MRILETARDA GRKYGIMLTM IYQSIGQMRE TYGGRDAASK WFESASWISF 550 560 570 580 590 600 AAINDPETAD YISRRCGMTT VEIDQVSRSF QTKGSSRTRS KQLAARPLIQ PHEVLRMRAD 610 620 630 640 EQIVFTAGNA PLRCGRAIWF RREDMKACGG EPVSQGRKDV

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB92442.1.
RefSeq	NP_443831.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55421.
ModBase	Search...
Protein-protein interaction databases
STRING	P55421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962282.
GenomeReviews	Gene locus NGR_a03950 in contig U00090_GR.
KEGG	rhi:NGR_a03950.
PATRIC	32301561. VBIRhiSp122450_0416.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299651.
OMA	NEVAPMV.
ProtClustDB	PRK13822.
Family and domain databases
InterPro	IPR014135. Ti-typ_conjug_TS_TraG. IPR003688. Ti-typ_conjug_TS_TraG-like. [Graphical view]
KO	K03205.
Pfam	PF02534. T4SS-DNA_transf. 1 hit. [Graphical view]
TIGRFAMs	TIGR02767. TraG-Ti. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRAG_RHISN

Accession

Primary (citable) accession number: P55421

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program