P55394 (TRAI_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acyl-homoserine-lactone synthase
EC=2.3.1.184

Alternative name(s):
Autoinducer synthesis protein traI

Gene names

Name:	traI
Ordered Locus Names:	NGR_a04220
ORF Names:	y4cL

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the synthesis of OHHL (N-(3-oxooctanoyl)-L-homoserine lactone), an autoinducer molecule which binds to traR and thus acts in the control of conjugal transfer By similarity.
Catalytic activity	An acyl-[acyl-carrier-protein] + S-adenosyl-L-methionine = [acyl-carrier-protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone.
Sequence similarities	Belongs to the autoinducer synthase family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Conjugation Quorum sensing
Molecular function	Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	conjugation Inferred from electronic annotation. Source: UniProtKB-KW quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Probable acyl-homoserine-lactone synthase		PRO_0000210900

Sequences

	Sequence		Length	Mass (Da)
	P55394 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: DF2F053C7A20D28E	FASTA	208	22,826
	10 20 30 40 50 60 MQILAISKPR NIEEAQLLRS HHELRARVFS DRLGWEVNVV GGCESDTFDD LQPTYILAVS 70 80 90 100 110 120 SNDRVVGCAR LLPALGPTMV ANVFPSLLSA GHLNAHSSMV ESSRFCVDTF LAESRGDGSI 130 140 150 160 170 180 HEATLTMFAG IIEWSVANRY TEIVTVTDLR FERILARVGW PLQRIGEPRP IGATVAVAGT 190 200 LPAKADTFMR LRPANYRSQI ISTFGQSA

References

[1]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[2]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB92427.1.
RefSeq	NP_443804.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55394.
ModBase	Search...
Protein-protein interaction databases
STRING	P55394.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962268.
GenomeReviews	Gene locus NGR_a04220 in contig U00090_GR.
KEGG	rhi:NGR_a04220.
PATRIC	32301617. VBIRhiSp122450_0444.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG368426.
OMA	YPNMITG.
ProtClustDB	PRK13834.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001690. Autoind_synth. IPR018311. Autoind_synth_CS. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00765. Autoind_synth. 1 hit. [Graphical view]
PRINTS	PR01549. AUTOINDCRSYN.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS00949. AUTOINDUCER_SYNTH_1. 1 hit. PS51187. AUTOINDUCER_SYNTH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRAI_RHISN

Accession

Primary (citable) accession number: P55394

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program