P55354 (NOEL_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GDP-mannose 4,6-dehydratase
EC=4.2.1.47

Alternative name(s):
GDP-D-mannose dehydratase

Gene names

Name:	noeL
Ordered Locus Names:	NGR_a00410
ORF Names:	y4aG

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O.
Cofactor	NAD.
Pathway	Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.
Sequence similarities	Belongs to the GDP-mannose 4,6-dehydratase family.

Ontologies

Keywords
Biological process	Nodulation
Ligand	NAD
Molecular function	Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	GDP-mannose metabolic process Inferred from electronic annotation. Source: InterPro nodulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GDP-mannose 4,6-dehydratase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	GDP-mannose 4,6-dehydratase		PRO_0000201721
Sites
	Active site	135	1	By similarity
	Active site	137	1	Nucleophile By similarity
	Active site	159	1	Nucleophile By similarity
	Binding site	163	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55354 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 5B0BD54DB3E5B8EF	FASTA	351	39,981
	10 20 30 40 50 60 MTDRKVALIS GVTGQDGAYL AELLLDEGYI VHGIKRRSSS FNTQRIEHIY QERHDPEARF 70 80 90 100 110 120 FLHYGDMTDS TNLLRIVQQT QPHEIYNLAA QSHVQVSFET PEYTANADAI GTLRMLEAIR 130 140 150 160 170 180 ILGLTNRTRF YQASTSELYG LAQESPQNEK TPFYPRSPYA AAKLYAYWIV VNYREAYGMH 190 200 210 220 230 240 ASNGILFNHE SPLRGETFVT RKITRAAAAI SLGKQEVLYL GNLDAQRDWG HAREYVRGMW 250 260 270 280 290 300 MMCQQDRPGD YVLATGVTTS VRTFVEWAFE ETGMTIEWVG EGIEERGIDA ATGRCVVAVD 310 320 330 340 350 PRYFRPTEVD LLLGDATKAR QVLGWRHETS VRDLACEMVR EDLSYLRGTR Q

References

[1]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[2]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91604.1.
RefSeq	NP_443766.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55354.
SMR	P55354. Positions 5-343.
ModBase	Search...
Protein-protein interaction databases
STRING	P55354.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962254.
GenomeReviews	Gene locus NGR_a00410 in contig U00090_GR.
KEGG	rhi:NGR_a00410.
PATRIC	32300805. VBIRhiSp122450_0038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG755066.
OMA	AYWIAIN.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR006368. GDP_Man_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01711.
PANTHER	PTHR10366:SF32. GDP_mann_dehyd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01472. Gmd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NOEL_RHISN

Accession

Primary (citable) accession number: P55354

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program