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Protein

GDP-L-fucose synthase

Gene

fcl

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.UniRule annotation

Catalytic activityi

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH.UniRule annotation

Pathwayi: GDP-L-fucose biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GDP-mannose 4,6-dehydratase (gmd)
  2. GDP-L-fucose synthase (fcl)
This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose, the pathway GDP-L-fucose biosynthesis via de novo pathway and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111Important for catalytic activityUniRule annotation1
Sitei113Important for catalytic activityUniRule annotation1
Active sitei140Proton donor/acceptorUniRule annotation1
Binding sitei144NADPUniRule annotation1
Binding sitei183NADPUniRule annotation1
Binding sitei191SubstrateUniRule annotation1
Binding sitei206SubstrateUniRule annotation1
Binding sitei213SubstrateUniRule annotation1
Binding sitei273SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 21NADPUniRule annotation7
Nucleotide bindingi109 – 112NADPUniRule annotation4
Nucleotide bindingi167 – 170NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Nodulation

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00128; UER00191.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-L-fucose synthaseUniRule annotation (EC:1.1.1.271UniRule annotation)
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductaseUniRule annotation
Gene namesi
Name:fclUniRule annotation
Ordered Locus Names:NGR_a00420
ORF Names:y4aF
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743601 – 314GDP-L-fucose synthaseAdd BLAST314

Structurei

3D structure databases

ProteinModelPortaliP55353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000168011.
KOiK02377.
OMAiIYRADFI.
OrthoDBiPOG091H0LVE.

Family and domain databases

CDDicd05239. GDP_FS_SDR_e. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose/colitose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P55353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMYLLDGKR IWVAGHKGMV GSAIIRSLAS EDCEVIVADR QKLDLTRQEE
60 70 80 90 100
VEKFLLKEKP HAVIMAAAKV GGILANDTMP ADFIYQNLIM EANVIEGSFR
110 120 130 140 150
SGVEKLLFLG SSCIYPKYAA QPIREEALLT GPLEPTNEWY AIAKIAGIKL
160 170 180 190 200
CQAYRKQYGA NFISAMPTNL YGPRDKFDLN SSHVVPALIR KAHEAKIKDL
210 220 230 240 250
GCLSIWGSGT PTRDFLYSED CSDALVFLLK HYSETEHINI GSGGEISIIE
260 270 280 290 300
LAHIVCRVVG FKGDIVFDTS KPDGTPRKLL SSERLVSMGW RPKTSLELGL
310
AKSYESFVSN VADN
Length:314
Mass (Da):34,674
Last modified:November 1, 1997 - v1
Checksum:i2E571D298AA98110
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91603.1.
RefSeqiNP_443765.1. NC_000914.2.
WP_010875084.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91603; AAB91603; NGR_a00420.
GeneIDi962636.
KEGGirhi:NGR_a00420.
PATRICi32300807. VBIRhiSp122450_0039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91603.1.
RefSeqiNP_443765.1. NC_000914.2.
WP_010875084.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55353.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91603; AAB91603; NGR_a00420.
GeneIDi962636.
KEGGirhi:NGR_a00420.
PATRICi32300807. VBIRhiSp122450_0039.

Phylogenomic databases

HOGENOMiHOG000168011.
KOiK02377.
OMAiIYRADFI.
OrthoDBiPOG091H0LVE.

Enzyme and pathway databases

UniPathwayiUPA00128; UER00191.

Family and domain databases

CDDicd05239. GDP_FS_SDR_e. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose/colitose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFCL_SINFN
AccessioniPrimary (citable) accession number: P55353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.