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P55353

- FCL_RHISN

UniProt

P55353 - FCL_RHISN

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Protein

GDP-L-fucose synthase

Gene

fcl

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.UniRule annotation

Catalytic activityi

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei111 – 1111Important for catalytic activityUniRule annotation
Sitei113 – 1131Important for catalytic activityUniRule annotation
Active sitei140 – 1401Proton donor/acceptorUniRule annotation
Binding sitei144 – 1441NADPUniRule annotation
Binding sitei183 – 1831NADPUniRule annotation
Binding sitei191 – 1911SubstrateUniRule annotation
Binding sitei206 – 2061SubstrateUniRule annotation
Binding sitei213 – 2131SubstrateUniRule annotation
Binding sitei273 – 2731SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 217NADPUniRule annotation
Nucleotide bindingi109 – 1124NADPUniRule annotation
Nucleotide bindingi167 – 1704NADPUniRule annotation

GO - Molecular functioni

  1. GDP-L-fucose synthase activity Source: UniProtKB-HAMAP
  2. isomerase activity Source: UniProtKB-KW
  3. NADP+ binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' GDP-L-fucose biosynthetic process Source: UniProtKB-HAMAP
  2. nodulation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Nodulation

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-795-MONOMER.
SFRE394:GBYN-6093-MONOMER.
UniPathwayiUPA00128; UER00191.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-L-fucose synthaseUniRule annotation (EC:1.1.1.271UniRule annotation)
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductaseUniRule annotation
Gene namesi
Name:fclUniRule annotation
Ordered Locus Names:NGR_a00420
ORF Names:y4aF
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314GDP-L-fucose synthasePRO_0000174360Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394.NGR_a00420.

Structurei

3D structure databases

ProteinModelPortaliP55353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0451.
HOGENOMiHOG000168011.
KOiK02377.
OMAiIHVMELD.
OrthoDBiEOG6JB132.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth.
InterProiIPR001509. Epimerase_deHydtase_N.
IPR028614. GDP_fucose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55353-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPMYLLDGKR IWVAGHKGMV GSAIIRSLAS EDCEVIVADR QKLDLTRQEE
60 70 80 90 100
VEKFLLKEKP HAVIMAAAKV GGILANDTMP ADFIYQNLIM EANVIEGSFR
110 120 130 140 150
SGVEKLLFLG SSCIYPKYAA QPIREEALLT GPLEPTNEWY AIAKIAGIKL
160 170 180 190 200
CQAYRKQYGA NFISAMPTNL YGPRDKFDLN SSHVVPALIR KAHEAKIKDL
210 220 230 240 250
GCLSIWGSGT PTRDFLYSED CSDALVFLLK HYSETEHINI GSGGEISIIE
260 270 280 290 300
LAHIVCRVVG FKGDIVFDTS KPDGTPRKLL SSERLVSMGW RPKTSLELGL
310
AKSYESFVSN VADN
Length:314
Mass (Da):34,674
Last modified:November 1, 1997 - v1
Checksum:i2E571D298AA98110
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91603.1.
RefSeqiNP_443765.1. NC_000914.2.
WP_010875084.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91603; AAB91603; NGR_a00420.
GeneIDi962636.
KEGGirhi:NGR_a00420.
PATRICi32300807. VBIRhiSp122450_0039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91603.1 .
RefSeqi NP_443765.1. NC_000914.2.
WP_010875084.1. NC_000914.2.

3D structure databases

ProteinModelPortali P55353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 394.NGR_a00420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB91603 ; AAB91603 ; NGR_a00420 .
GeneIDi 962636.
KEGGi rhi:NGR_a00420.
PATRICi 32300807. VBIRhiSp122450_0039.

Phylogenomic databases

eggNOGi COG0451.
HOGENOMi HOG000168011.
KOi K02377.
OMAi IHVMELD.
OrthoDBi EOG6JB132.

Enzyme and pathway databases

UniPathwayi UPA00128 ; UER00191 .
BioCyci RETL1328306-WGS:GSTH-795-MONOMER.
SFRE394:GBYN-6093-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00956. GDP_fucose_synth.
InterProi IPR001509. Epimerase_deHydtase_N.
IPR028614. GDP_fucose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01370. Epimerase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiFCL_RHISN
AccessioniPrimary (citable) accession number: P55353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3