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P55353

- FCL_RHISN

UniProt

P55353 - FCL_RHISN

Protein

GDP-L-fucose synthase

Gene

fcl

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.UniRule annotation

    Catalytic activityi

    GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei111 – 1111Important for catalytic activityUniRule annotation
    Sitei113 – 1131Important for catalytic activityUniRule annotation
    Active sitei140 – 1401Proton donor/acceptorUniRule annotation
    Binding sitei144 – 1441NADPUniRule annotation
    Binding sitei183 – 1831NADPUniRule annotation
    Binding sitei191 – 1911SubstrateUniRule annotation
    Binding sitei206 – 2061SubstrateUniRule annotation
    Binding sitei213 – 2131SubstrateUniRule annotation
    Binding sitei273 – 2731SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 217NADPUniRule annotation
    Nucleotide bindingi109 – 1124NADPUniRule annotation
    Nucleotide bindingi167 – 1704NADPUniRule annotation

    GO - Molecular functioni

    1. GDP-L-fucose synthase activity Source: UniProtKB-HAMAP
    2. isomerase activity Source: UniProtKB-KW
    3. NADP+ binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' GDP-L-fucose biosynthetic process Source: UniProtKB-HAMAP
    2. nodulation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Oxidoreductase

    Keywords - Biological processi

    Nodulation

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-795-MONOMER.
    SFRE394:GBYN-6093-MONOMER.
    UniPathwayiUPA00128; UER00191.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GDP-L-fucose synthaseUniRule annotation (EC:1.1.1.271UniRule annotation)
    Alternative name(s):
    GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductaseUniRule annotation
    Gene namesi
    Name:fclUniRule annotation
    Ordered Locus Names:NGR_a00420
    ORF Names:y4aF
    Encoded oniPlasmid sym pNGR234a0 Publication
    OrganismiRhizobium sp. (strain NGR234)
    Taxonomic identifieri394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001054: Plasmid sym pNGR234a

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314GDP-L-fucose synthasePRO_0000174360Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi394.NGR_a00420.

    Structurei

    3D structure databases

    ProteinModelPortaliP55353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0451.
    HOGENOMiHOG000168011.
    KOiK02377.
    OMAiIHVMELD.
    OrthoDBiEOG6JB132.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00956. GDP_fucose_synth.
    InterProiIPR001509. Epimerase_deHydtase.
    IPR028614. GDP_fucose_synth.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01370. Epimerase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55353-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPMYLLDGKR IWVAGHKGMV GSAIIRSLAS EDCEVIVADR QKLDLTRQEE    50
    VEKFLLKEKP HAVIMAAAKV GGILANDTMP ADFIYQNLIM EANVIEGSFR 100
    SGVEKLLFLG SSCIYPKYAA QPIREEALLT GPLEPTNEWY AIAKIAGIKL 150
    CQAYRKQYGA NFISAMPTNL YGPRDKFDLN SSHVVPALIR KAHEAKIKDL 200
    GCLSIWGSGT PTRDFLYSED CSDALVFLLK HYSETEHINI GSGGEISIIE 250
    LAHIVCRVVG FKGDIVFDTS KPDGTPRKLL SSERLVSMGW RPKTSLELGL 300
    AKSYESFVSN VADN 314
    Length:314
    Mass (Da):34,674
    Last modified:November 1, 1997 - v1
    Checksum:i2E571D298AA98110
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00090 Genomic DNA. Translation: AAB91603.1.
    RefSeqiNP_443765.1. NC_000914.2.

    Genome annotation databases

    EnsemblBacteriaiAAB91603; AAB91603; NGR_a00420.
    GeneIDi962636.
    KEGGirhi:NGR_a00420.
    PATRICi32300807. VBIRhiSp122450_0039.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00090 Genomic DNA. Translation: AAB91603.1 .
    RefSeqi NP_443765.1. NC_000914.2.

    3D structure databases

    ProteinModelPortali P55353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394.NGR_a00420.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB91603 ; AAB91603 ; NGR_a00420 .
    GeneIDi 962636.
    KEGGi rhi:NGR_a00420.
    PATRICi 32300807. VBIRhiSp122450_0039.

    Phylogenomic databases

    eggNOGi COG0451.
    HOGENOMi HOG000168011.
    KOi K02377.
    OMAi IHVMELD.
    OrthoDBi EOG6JB132.

    Enzyme and pathway databases

    UniPathwayi UPA00128 ; UER00191 .
    BioCyci RETL1328306-WGS:GSTH-795-MONOMER.
    SFRE394:GBYN-6093-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00956. GDP_fucose_synth.
    InterProi IPR001509. Epimerase_deHydtase.
    IPR028614. GDP_fucose_synth.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01370. Epimerase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular basis of symbiosis between Rhizobium and legumes."
      Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
      Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NGR234.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NGR234.

    Entry informationi

    Entry nameiFCL_RHISN
    AccessioniPrimary (citable) accession number: P55353
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

    Documents

    1. Rhizobium plasmid pNGR234a
      Rhizobium plasmid pNGR234a: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3