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P55353 (FCL_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GDP-L-fucose synthase

EC=1.1.1.271
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
Gene names
Name:fcl
Ordered Locus Names:NGR_a00420
ORF Names:y4aF
Encoded onPlasmid sym pNGR234a
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction By similarity. HAMAP-Rule MF_00956

Catalytic activity

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH. HAMAP-Rule MF_00956

Pathway

Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2. HAMAP-Rule MF_00956

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.

Ontologies

Keywords
   Biological processNodulation
   LigandNADP
   Molecular functionIsomerase
Oxidoreductase
   Technical termComplete proteome
Multifunctional enzyme
Plasmid
Gene Ontology (GO)
   Biological_process'de novo' GDP-L-fucose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nodulation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionGDP-L-fucose synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

NADP+ binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

isomerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314GDP-L-fucose synthase HAMAP-Rule MF_00956
PRO_0000174360

Regions

Nucleotide binding15 – 217NADP By similarity
Nucleotide binding109 – 1124NADP By similarity
Nucleotide binding167 – 1704NADP By similarity

Sites

Active site1401Proton donor/acceptor By similarity
Binding site1441NADP By similarity
Binding site1831NADP By similarity
Binding site1911Substrate By similarity
Binding site2061Substrate By similarity
Binding site2131Substrate By similarity
Binding site2731Substrate By similarity
Site1111Important for catalytic activity By similarity
Site1131Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P55353 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 2E571D298AA98110

FASTA31434,674
        10         20         30         40         50         60 
MPMYLLDGKR IWVAGHKGMV GSAIIRSLAS EDCEVIVADR QKLDLTRQEE VEKFLLKEKP 

        70         80         90        100        110        120 
HAVIMAAAKV GGILANDTMP ADFIYQNLIM EANVIEGSFR SGVEKLLFLG SSCIYPKYAA 

       130        140        150        160        170        180 
QPIREEALLT GPLEPTNEWY AIAKIAGIKL CQAYRKQYGA NFISAMPTNL YGPRDKFDLN 

       190        200        210        220        230        240 
SSHVVPALIR KAHEAKIKDL GCLSIWGSGT PTRDFLYSED CSDALVFLLK HYSETEHINI 

       250        260        270        280        290        300 
GSGGEISIIE LAHIVCRVVG FKGDIVFDTS KPDGTPRKLL SSERLVSMGW RPKTSLELGL 

       310 
AKSYESFVSN VADN 

« Hide

References

[1]"Molecular basis of symbiosis between Rhizobium and legumes."
Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[2]"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems."
Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.
Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00090 Genomic DNA. Translation: AAB91603.1.
RefSeqNP_443765.1. NC_000914.2.

3D structure databases

ProteinModelPortalP55353.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_a00420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91603; AAB91603; NGR_a00420.
GeneID962636.
KEGGrhi:NGR_a00420.
PATRIC32300807. VBIRhiSp122450_0039.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0451.
HOGENOMHOG000168011.
KOK02377.
OMAIHCAGRV.
OrthoDBEOG6JB132.

Enzyme and pathway databases

BioCycSFRE394:GBYN-6093-MONOMER.
UniPathwayUPA00128; UER00191.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00956. GDP_fucose_synth.
InterProIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFCL_RHISN
AccessionPrimary (citable) accession number: P55353
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names