Reviewed, UniProtKB/Swiss-Prot P55350 (Y4AC_RHISN)

Names and origin

Protein names

Recommended name:
    Putative phytoene synthase
    EC=2.5.1.32

Gene names

Ordered Locus Names:	NGR_a00440
ORF Names:	y4aC

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene. Probably part of an operon y4aABCD involved in the synthesis of an isoprenoid compound.
Catalytic activity	2 geranylgeranyl diphosphate = diphosphate + prephytoene diphosphate. Prephytoene diphosphate = diphosphate + phytoene.
Sequence similarities	Belongs to the phytoene/squalene synthetase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme Plasmid
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Putative phytoene synthase		PRO_0000067442

Sequences

	Sequence		Length	Mass (Da)
	P55350-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: DD236CB8F72BAFC5	FASTA	279	31,354
	10 20 30 40 50 60 MRAEAAAHAN HRSTALGSSF YLGMRTLPPV QREAIFQIYS FCRQVDDIAD SDEPREHRLA 70 80 90 100 110 120 ALQQWRDHID ALYQCVPPPR LKDYLASVTT FGLKREDFLA VVDGMEMDVL QDIRAPKMAT 130 140 150 160 170 180 LDLYCDRVAS AVGRMSVRVF GLSEEDGIAL AHHLGRALQL TNILRDIDED AGLGRLYIPR 190 200 210 220 230 240 ESLDHAGITS SDPHKVIADK ALPKACAPLA QRAMMHFAES DEIMNCNPRR IVRAPTIMSK 250 260 270 CYRAILDLLL IRGFAAPREP VRVTNLTKRA ILFRYALML

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00090 Genomic DNA. Translation: AAB91601.1.
RefSeq	NP_443763.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	962247.
GenomeReviews	Gene locus NGR_a00440 in contig U00090_GR.
KEGG	rhi:NGR_a00440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG693191.
Family and domain databases
InterPro	IPR002060. Squ/phyt_synthse. IPR017828. Squalene/phytoene_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y4AC_RHISN

Accession

Primary (citable) accession number: P55350

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)