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Protein

Putative phytoene synthase

Gene

NGR_a00440

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reaction from prephytoene diphosphate to phytoene. Probably part of an operon y4aABCD involved in the synthesis of an isoprenoid compound.

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis, Isoprene biosynthesis

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6095-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative phytoene synthase (EC:2.5.1.32)
Gene namesi
Ordered Locus Names:NGR_a00440
ORF Names:y4aC
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Putative phytoene synthasePRO_0000067442Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394.NGR_a00440.

Structurei

3D structure databases

ProteinModelPortaliP55350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220850.
KOiK02291.
OMAiVKAPRIM.
OrthoDBiEOG6PGK3F.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR017828. Squalene/phytoene_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03465. HpnD. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAEAAAHAN HRSTALGSSF YLGMRTLPPV QREAIFQIYS FCRQVDDIAD
60 70 80 90 100
SDEPREHRLA ALQQWRDHID ALYQCVPPPR LKDYLASVTT FGLKREDFLA
110 120 130 140 150
VVDGMEMDVL QDIRAPKMAT LDLYCDRVAS AVGRMSVRVF GLSEEDGIAL
160 170 180 190 200
AHHLGRALQL TNILRDIDED AGLGRLYIPR ESLDHAGITS SDPHKVIADK
210 220 230 240 250
ALPKACAPLA QRAMMHFAES DEIMNCNPRR IVRAPTIMSK CYRAILDLLL
260 270
IRGFAAPREP VRVTNLTKRA ILFRYALML
Length:279
Mass (Da):31,354
Last modified:November 1, 1997 - v1
Checksum:iDD236CB8F72BAFC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91601.1.
RefSeqiNP_443763.1. NC_000914.2.
WP_010875086.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91601; AAB91601; NGR_a00440.
GeneIDi962247.
KEGGirhi:NGR_a00440.
PATRICi32300811. VBIRhiSp122450_0041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA. Translation: AAB91601.1.
RefSeqiNP_443763.1. NC_000914.2.
WP_010875086.1. NC_000914.2.

3D structure databases

ProteinModelPortaliP55350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_a00440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91601; AAB91601; NGR_a00440.
GeneIDi962247.
KEGGirhi:NGR_a00440.
PATRICi32300811. VBIRhiSp122450_0041.

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220850.
KOiK02291.
OMAiVKAPRIM.
OrthoDBiEOG6PGK3F.

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6095-MONOMER.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR017828. Squalene/phytoene_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03465. HpnD. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiY4AC_RHISN
AccessioniPrimary (citable) accession number: P55350
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.