P55348 (SQHC_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable squalene--hopene cyclase
EC=4.2.1.129
EC=5.4.99.17

Alternative name(s):
Squalene--hopanol cyclase

Gene names

Name:	shc
Ordered Locus Names:	NGR_a00460
ORF Names:	y4aA

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the cyclization of squalene into hopene. Probably part of an operon y4aABCD involved in the synthesis of an isoprenoid compound.
Catalytic activity	Squalene = hop-22(29)-ene. Hopan-22-ol = squalene + H2O.
Pathway	Secondary metabolite biosynthesis; hopanoid biosynthesis.
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 3 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	hopanoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW squalene-hopene cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	Probable squalene--hopene cyclase		PRO_0000072652
Regions
	Repeat	67 – 108	42	PFTB 1
	Repeat	413 – 454	42	PFTB 2
	Repeat	530 – 577	48	PFTB 3

Sequences

	Sequence		Length	Mass (Da)
	P55348 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: BD0C336905D1ECAE	FASTA	647	72,130
	10 20 30 40 50 60 MNKHSGNRTA IDPAALEMSI ASATEALLAY RHADGHWAFE LEADSTIPSE YILLRHYLAE 70 80 90 100 110 120 PIDVVLEAKI GNYLRRTQGA HGGWPLVHDG PFDMSASVKS YFALKMIGDS VDAAHMVKAR 130 140 150 160 170 180 EAIRARGGAA NSNVLTRFLL ALYGVVSWRA VPVLPIEIVL LPIWSPFHLY KISYWARTTI 190 200 210 220 230 240 VPLMVLAVLK PRAKNPKGVG IEELFLQDTK SVGMNPKAPH QSWGWFLLFR GIDGILRVIE 250 260 270 280 290 300 PHLPKKLRER AIASALAFTE ERLNGEDGMG AIYPSMANIV MMYDALGKDD HFPPRAIARR 310 320 330 340 350 360 AIDKLLVIGE EEAYCQPCLS PVWDTALTCH ALQEVGGANA VAKAKQGLDW LKPRQVLDVK 370 380 390 400 410 420 GDWAVKAPNI RPGGWPFQYN NAHYPDLDDT AVVVMAMDRA QRHAGSKEYA TAIARGREWI 430 440 450 460 470 480 EGMQSRDGGW AAFDVNNLEY YLNNLPFADH GALLDPPTED VTARCVSMLA QVGEFTQRSK 490 500 510 520 530 540 AVAEGIAYLR RTQHAEGSWY GRWGLNYIYG TWSVLCALNA AGIDHQDPMI RKAVEWLVSI 550 560 570 580 590 600 QSWDGGWGED AISYRLDYSG YEQAPSTSSQ TAWALLGLMA AGEVEHPAVA RGVNYLKNAQ 610 620 630 640 TENGLWDEQR YTATGFPRVF YLRYHGYSKF FPLWALARYR NLRSTNV

References

[1]

"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

[2]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00090 Genomic DNA. Translation: AAB91964.1.
RefSeq	NP_443761.1. NC_000914.2.
3D structure databases
ProteinModelPortal	P55348.
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a00460.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91964; AAB91964; NGR_a00460.
GeneID	962250.
KEGG	rhi:NGR_a00460.
PATRIC	32300815. VBIRhiSp122450_0043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1657.
HOGENOM	HOG000220823.
KO	K06045.
OMA	RRIQGAH.
ProtClustDB	CLSK2393506.
Enzyme and pathway databases
UniPathway	UPA00337.
Family and domain databases
InterPro	IPR006400. Hopene-cyclase. IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01507. hopene_cyclase. 1 hit. TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SQHC_RHISN

Accession

Primary (citable) accession number: P55348

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program