Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein PKNOX1

Gene

PKNOX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi259 – 321Homeobox; TALE-typePROSITE-ProRule annotationAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000160199-MONOMER.
ReactomeiR-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
SIGNORiP55347.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein PKNOX1
Alternative name(s):
Homeobox protein PREP-1
PBX/knotted homeobox 1
Gene namesi
Name:PKNOX1
Synonyms:PREP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:9022. PKNOX1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5316.
OpenTargetsiENSG00000160199.
PharmGKBiPA33354.

Polymorphism and mutation databases

DMDMi115311619.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000492481 – 436Homeobox protein PKNOX1Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineBy similarity1
Modified residuei41PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55347.
PaxDbiP55347.
PeptideAtlasiP55347.
PRIDEiP55347.

2D gel databases

SWISS-2DPAGEP55347.

PTM databases

iPTMnetiP55347.
PhosphoSitePlusiP55347.

Expressioni

Tissue specificityi

Ubiquitous. Isoform 2 is expressed in all examined tissues except in bone marrow.2 Publications

Gene expression databases

BgeeiENSG00000160199.
CleanExiHS_PKNOX1.
ExpressionAtlasiP55347. baseline and differential.
GenevisibleiP55347. HS.

Organism-specific databases

HPAiCAB004285.
HPA057215.
HPA065017.

Interactioni

Protein-protein interaction databases

BioGridi111333. 21 interactors.
IntActiP55347. 14 interactors.
MINTiMINT-1532511.
STRINGi9606.ENSP00000291547.

Structurei

Secondary structure

1436
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi268 – 280Combined sources13
Turni281 – 283Combined sources3
Helixi289 – 299Combined sources11
Helixi303 – 319Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X2NNMR-A260-319[»]
ProteinModelPortaliP55347.
SMRiP55347.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55347.

Family & Domainsi

Sequence similaritiesi

Belongs to the TALE/MEIS homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
GeneTreeiENSGT00550000074260.
HOGENOMiHOG000253922.
HOVERGENiHBG055193.
InParanoidiP55347.
OMAiMMAEQSE.
OrthoDBiEOG091G09WJ.
PhylomeDBiP55347.
TreeFamiTF318093.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR032453. PKNOX/Meis_N.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
PF16493. Meis_PKNOX_N. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P55347-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP
60 70 80 90 100
MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ
110 120 130 140 150
EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL
160 170 180 190 200
KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN
210 220 230 240 250
VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS
260 270 280 290 300
ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN
310 320 330 340 350
LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRFWPDSI
360 370 380 390 400
ASGVAQPPPS ELTMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM
410 420 430
AGQSEDESVD STEEDAGALA PAHISGLVLE NSDSLQ
Length:436
Mass (Da):47,607
Last modified:September 19, 2006 - v3
Checksum:i381E901F435AC5F2
GO
Isoform 2 (identifier: P55347-2) [UniParc]FASTAAdd to basket
Also known as: PKNOX1B

The sequence of this isoform differs from the canonical sequence as follows:
     368-436: AVVTITTPVN...LVLENSDSLQ → TGGRPRPDMV...SHRARPRMTS

Show »
Length:405
Mass (Da):44,629
Checksum:i2413564513C5DE76
GO

Sequence cautioni

The sequence AAC51243 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA95533 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153K → E in CAA73934 (PubMed:9479508).Curated1
Sequence conflicti347P → A in AAC51243 (PubMed:9143494).Curated1
Sequence conflicti430E → A in CAA73934 (PubMed:9479508).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049588126R → H.Corresponds to variant rs9976017dbSNPEnsembl.1
Natural variantiVAR_049589216T → A.Corresponds to variant rs17115709dbSNPEnsembl.1
Natural variantiVAR_036440265V → I in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs376883451dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017260368 – 436AVVTI…SDSLQ → TGGRPRPDMVDHGVGIMNKR PGSGLLAVSHRARPRMTS in isoform 2. 1 PublicationAdd BLAST69

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68727 mRNA. Translation: AAC51243.1. Different initiation.
Y13613 mRNA. Translation: CAA73934.1.
AY142115 mRNA. Translation: AAN34940.1.
AP001748 Genomic DNA. Translation: BAA95533.1. Different initiation.
CCDSiCCDS13692.1. [P55347-1]
RefSeqiNP_004562.2. NM_004571.4. [P55347-1]
UniGeneiHs.431043.

Genome annotation databases

EnsembliENST00000291547; ENSP00000291547; ENSG00000160199. [P55347-1]
GeneIDi5316.
KEGGihsa:5316.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68727 mRNA. Translation: AAC51243.1. Different initiation.
Y13613 mRNA. Translation: CAA73934.1.
AY142115 mRNA. Translation: AAN34940.1.
AP001748 Genomic DNA. Translation: BAA95533.1. Different initiation.
CCDSiCCDS13692.1. [P55347-1]
RefSeqiNP_004562.2. NM_004571.4. [P55347-1]
UniGeneiHs.431043.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X2NNMR-A260-319[»]
ProteinModelPortaliP55347.
SMRiP55347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111333. 21 interactors.
IntActiP55347. 14 interactors.
MINTiMINT-1532511.
STRINGi9606.ENSP00000291547.

PTM databases

iPTMnetiP55347.
PhosphoSitePlusiP55347.

Polymorphism and mutation databases

DMDMi115311619.

2D gel databases

SWISS-2DPAGEP55347.

Proteomic databases

EPDiP55347.
PaxDbiP55347.
PeptideAtlasiP55347.
PRIDEiP55347.

Protocols and materials databases

DNASUi5316.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291547; ENSP00000291547; ENSG00000160199. [P55347-1]
GeneIDi5316.
KEGGihsa:5316.

Organism-specific databases

CTDi5316.
DisGeNETi5316.
GeneCardsiPKNOX1.
HGNCiHGNC:9022. PKNOX1.
HPAiCAB004285.
HPA057215.
HPA065017.
MIMi602100. gene.
neXtProtiNX_P55347.
OpenTargetsiENSG00000160199.
PharmGKBiPA33354.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
GeneTreeiENSGT00550000074260.
HOGENOMiHOG000253922.
HOVERGENiHBG055193.
InParanoidiP55347.
OMAiMMAEQSE.
OrthoDBiEOG091G09WJ.
PhylomeDBiP55347.
TreeFamiTF318093.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000160199-MONOMER.
ReactomeiR-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
SIGNORiP55347.

Miscellaneous databases

ChiTaRSiPKNOX1. human.
EvolutionaryTraceiP55347.
GeneWikiiPKNOX1.
GenomeRNAii5316.
PROiP55347.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160199.
CleanExiHS_PKNOX1.
ExpressionAtlasiP55347. baseline and differential.
GenevisibleiP55347. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR032453. PKNOX/Meis_N.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
PF16493. Meis_PKNOX_N. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKNX1_HUMAN
AccessioniPrimary (citable) accession number: P55347
Secondary accession number(s): O00528, Q8IWT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 19, 2006
Last modified: November 30, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.