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Protein

Protein arginine N-methyltransferase 2

Gene

PRMT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.4 Publications

Catalytic activityi

2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine.1 Publication

Kineticsi

  1. KM=2.6 µM for AdoMet1 Publication
  2. KM=3.3 µM for H41 Publication
  1. Vmax=1.4 nmol/min/mg enzyme toward AdoMet1 Publication
  2. Vmax=1.5 nmol/min/mg enzyme toward H41 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112S-adenosyl-L-methionineBy similarity1
Binding sitei121S-adenosyl-L-methionineBy similarity1
Binding sitei145S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei168S-adenosyl-L-methionineBy similarity1
Binding sitei197S-adenosyl-L-methionineBy similarity1
Active sitei211By similarity1
Active sitei220By similarity1

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • histone-arginine N-methyltransferase activity Source: UniProtKB
  • histone methyltransferase activity Source: UniProtKB
  • peroxisome proliferator activated receptor binding Source: UniProtKB
  • progesterone receptor binding Source: UniProtKB
  • protein-arginine N-methyltransferase activity Source: UniProtKB
  • protein-arginine omega-N asymmetric methyltransferase activity Source: GO_Central
  • protein homodimerization activity Source: UniProtKB
  • retinoic acid receptor binding Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

  • developmental cell growth Source: UniProtKB
  • histone methylation Source: UniProtKB
  • negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
  • negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • protein methylation Source: ProtInc
  • regulation of androgen receptor signaling pathway Source: UniProtKB
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.125 2681

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 2 (EC:2.1.1.3191 Publication)
Alternative name(s):
Histone-arginine N-methyltransferase PRMT2
Gene namesi
Name:PRMT2
Synonyms:HMT1, HRMT1L1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000160310.16
HGNCiHGNC:5186 PRMT2
MIMi601961 gene
neXtProtiNX_P55345

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3275
OpenTargetsiENSG00000160310
PharmGKBiPA29460

Polymorphism and mutation databases

DMDMi2499805

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123241 – 433Protein arginine N-methyltransferase 2Add BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61Asymmetric dimethylarginineCombined sources1
Modified residuei72Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP55345
PeptideAtlasiP55345
PRIDEiP55345
ProteomicsDBi56853
56854 [P55345-2]
56855 [P55345-3]

PTM databases

iPTMnetiP55345
PhosphoSitePlusiP55345
SwissPalmiP55345

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.1 Publication

Gene expression databases

BgeeiENSG00000160310
CleanExiHS_PRMT2
ExpressionAtlasiP55345 baseline and differential
GenevisibleiP55345 HS

Organism-specific databases

HPAiHPA018976
HPA029591

Interactioni

Subunit structurei

Self-associates. Interacts with RB1 and E2F1 (By similarity). Interacts with NCOA6 coactivator. Interacts (via SH3 domain) with PRMT8. Interacts with AR. Interacts with NFKBIA. Interacts with ESR1, ESR2, PGR, PPARG, RARA, RXRA and THRB. Interacts with HNRNPUL1.By similarity6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • peroxisome proliferator activated receptor binding Source: UniProtKB
  • progesterone receptor binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • retinoic acid receptor binding Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109511, 52 interactors
CORUMiP55345
IntActiP55345, 25 interactors
MINTiP55345
STRINGi9606.ENSP00000347906

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 39Combined sources6
Beta strandi56 – 61Combined sources6
Beta strandi64 – 71Combined sources8
Beta strandi77 – 85Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X2PNMR-A33-87[»]
ProteinModelPortaliP55345
SMRiP55345
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55345

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 89SH3PROSITE-ProRule annotationAdd BLAST60
Domaini99 – 432SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST334

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 277Interaction with ESR1Add BLAST277
Regioni83 – 207Interaction with RB1By similarityAdd BLAST125
Regioni133 – 275Interaction with ESR1Add BLAST143

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG1499 Eukaryota
ENOG410XQYH LUCA
GeneTreeiENSGT00550000074406
HOGENOMiHOG000198521
HOVERGENiHBG105734
InParanoidiP55345
KOiK11435
OMAiPCTILQL
OrthoDBiEOG091G0A0U
PhylomeDBiP55345
TreeFamiTF332196

Family and domain databases

InterProiView protein in InterPro
IPR025799 Arg_MeTrfase
IPR029063 SAM-dependent_MTases
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR007848 Small_mtfrase_dom
PfamiView protein in Pfam
PF05175 MTS, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51678 SAM_MT_PRMT, 1 hit
PS50002 SH3, 1 hit

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P55345-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATSGDCPRS ESQGEEPAEC SEAGLLQEGV QPEEFVAIAD YAATDETQLS
60 70 80 90 100
FLRGEKILIL RQTTADWWWG ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE
110 120 130 140 150
EYFGSYGTLK LHLEMLADQP RTTKYHSVIL QNKESLTDKV ILDVGCGTGI
160 170 180 190 200
ISLFCAHYAR PRAVYAVEAS EMAQHTGQLV LQNGFADIIT VYQQKVEDVV
210 220 230 240 250
LPEKVDVLVS EWMGTCLLFE FMIESILYAR DAWLKEDGVI WPTMAALHLV
260 270 280 290 300
PCSADKDYRS KVLFWDNAYE FNLSALKSLA VKEFFSKPKY NHILKPEDCL
310 320 330 340 350
SEPCTILQLD MRTVQISDLE TLRGELRFDI RKAGTLHGFT AWFSVHFQSL
360 370 380 390 400
QEGQPPQVLS TGPFHPTTHW KQTLFMMDDP VPVHTGDVVT GSVVLQRNPV
410 420 430
WRRHMSVALS WAVTSRQDPT SQKVGEKVFP IWR
Length:433
Mass (Da):49,042
Last modified:November 1, 1997 - v1
Checksum:i6DEE0350C15ECD4F
GO
Isoform 2 (identifier: P55345-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-433: FEFMIESILY...VGEKVFPIWR → AAPLLSCRIL...LHLSWPIFLL

Note: No experimental confirmation available.
Show »
Length:284
Mass (Da):31,162
Checksum:i83703F72DDFA885B
GO
Isoform 3 (identifier: P55345-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-320: Missing.

Note: No experimental confirmation available.
Show »
Length:331
Mass (Da):37,167
Checksum:iB3FDADFE7F9133E9
GO
Isoform PRMT2Alpha (identifier: P55345-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-289: SLAVKEFFSKPK → LEKKSSPSGDDS
     290-433: Missing.

Note: Higher expression in breast cancer tissues.
Show »
Length:289
Mass (Da):32,424
Checksum:i49DEAA4267BF98ED
GO
Isoform PRMT2Beta (identifier: P55345-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-301: FEFMIESILY...HILKPEDCLS → HHTLEADAVH...FGKQCAYLEG
     302-433: Missing.

Note: Higher expression in breast cancer tissues. Doesn't interact with ESR1.
Show »
Length:301
Mass (Da):33,543
Checksum:iFBEC6C51A1CE8B64
GO
Isoform PRMT2Gamma (identifier: P55345-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-423: Missing.

Note: Higher expression in breast cancer tissues.
Show »
Length:228
Mass (Da):25,494
Checksum:iC02A5A20FB63338C
GO
Isoform PRMT2L2 (identifier: P55345-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-433: Missing.

Show »
Length:277
Mass (Da):31,193
Checksum:i970E9AD5A09EB8CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256 – 260KDYRS → RIIVA in AAB48437 (PubMed:9196002).Curated5
Sequence conflicti402R → I in AAH00727 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042680219 – 433FEFMI…FPIWR → AAPLLSCRILPCTCASGPLH VLLACCLPLPCTCASVPLHV LLACCLPVLRAPQPSGLHLS WPIFLL in isoform 2. 1 PublicationAdd BLAST215
Alternative sequenceiVSP_054927219 – 423Missing in isoform PRMT2Gamma. 1 PublicationAdd BLAST205
Alternative sequenceiVSP_043381219 – 320Missing in isoform 3. 1 PublicationAdd BLAST102
Alternative sequenceiVSP_054928219 – 301FEFMI…EDCLS → HHTLEADAVHDGRPSPCPYR RRGHGFSCVAEKPSVEKAHV CGSELGCHFQTRPHISKSWR KSLPHLEMTVDALFGKQCAY LEG in isoform PRMT2Beta. 1 PublicationAdd BLAST83
Alternative sequenceiVSP_054929278 – 433Missing in isoform PRMT2L2. 1 PublicationAdd BLAST156
Alternative sequenceiVSP_054930278 – 289SLAVK…FSKPK → LEKKSSPSGDDS in isoform PRMT2Alpha. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_054931290 – 433Missing in isoform PRMT2Alpha. 1 PublicationAdd BLAST144
Alternative sequenceiVSP_054932302 – 433Missing in isoform PRMT2Beta. 1 PublicationAdd BLAST132

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99209 mRNA Translation: CAA67599.1
U80213 mRNA Translation: AAB48437.1
AY786414 mRNA Translation: AAV48568.2
FJ436410 mRNA Translation: ACJ66866.1
FJ436411 mRNA Translation: ACJ66867.1
FJ436412 mRNA Translation: ACJ66868.1
U79286 mRNA Translation: AAB50221.1
CR541804 mRNA Translation: CAG46603.1
AK123650 mRNA Translation: BAG53931.1
AP000339 Genomic DNA No translation available.
AP000340 Genomic DNA No translation available.
CH471079 Genomic DNA Translation: EAX09259.1
CH471079 Genomic DNA Translation: EAX09265.1
BC000727 mRNA Translation: AAH00727.1
BC100026 mRNA Translation: AAI00027.1
AL109794 mRNA Translation: CAB52454.1
CCDSiCCDS13737.1 [P55345-1]
CCDS56219.1 [P55345-2]
CCDS56220.1 [P55345-3]
CCDS68230.1 [P55345-4]
CCDS68231.1 [P55345-5]
CCDS74806.1 [P55345-6]
RefSeqiNP_001229793.1, NM_001242864.2 [P55345-3]
NP_001229794.1, NM_001242865.2 [P55345-2]
NP_001229795.1, NM_001242866.2 [P55345-7]
NP_001273605.1, NM_001286676.1 [P55345-5]
NP_001273606.1, NM_001286677.1 [P55345-4]
NP_001273607.1, NM_001286678.1 [P55345-6]
NP_001526.2, NM_001535.4 [P55345-1]
NP_996845.1, NM_206962.3 [P55345-1]
XP_005261168.1, XM_005261111.3 [P55345-1]
XP_006724061.1, XM_006723998.3 [P55345-5]
XP_006724063.1, XM_006724000.2 [P55345-6]
UniGeneiHs.154163
Hs.661229

Genome annotation databases

EnsembliENST00000291705; ENSP00000291705; ENSG00000160310 [P55345-6]
ENST00000334494; ENSP00000335490; ENSG00000160310 [P55345-2]
ENST00000355680; ENSP00000347906; ENSG00000160310 [P55345-1]
ENST00000397637; ENSP00000380759; ENSG00000160310 [P55345-1]
ENST00000397638; ENSP00000380760; ENSG00000160310 [P55345-1]
ENST00000440086; ENSP00000397266; ENSG00000160310 [P55345-3]
ENST00000451211; ENSP00000411984; ENSG00000160310 [P55345-4]
ENST00000458387; ENSP00000407463; ENSG00000160310 [P55345-5]
GeneIDi3275
KEGGihsa:3275
UCSCiuc002zjx.5 human [P55345-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiANM2_HUMAN
AccessioniPrimary (citable) accession number: P55345
Secondary accession number(s): B7U630
, B7U631, B7U632, P78350, Q498Y5, Q5U7D4, Q6FHF0, Q99781, Q9BW15, Q9UMC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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