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P55332

- XYNA_EMENI

UniProt

P55332 - XYNA_EMENI

Protein

Endo-1,4-beta-xylanase A

Gene

xlnA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.2 Publications

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

    pH dependencei

    Optimum pH is 5.4.1 Publication

    Temperature dependencei

    Optimum temperature is 52 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei121 – 1211NucleophilePROSITE-ProRule annotation
    Active sitei212 – 2121Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiGH11. Glycoside Hydrolase Family 11.
    mycoCLAPiXYN11A_EMENI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase A (EC:3.2.1.8)
    Short name:
    Xylanase A
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A
    22 kDa xylanase
    Xylanase X22
    Gene namesi
    Name:xlnA
    ORF Names:AN3613
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome II

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Biotechnological usei

    An increase in fruity aroma was organoleptically detected in the wine produced by the recombinant wine yeast strain T73 expressing xnlA.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 225206Endo-1,4-beta-xylanase APRO_0000008004Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the presence of D-xylose under conditions of alkaline ambient pH, probably under the regulation of the pacC transcription factor. Repressed in presence of glucose through the action of the creA transcription repressor.3 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliP55332.
    SMRiP55332. Positions 39-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG05353.
    HOGENOMiHOG000179135.
    OMAiNETALHE.
    OrthoDBiEOG7VQJQX.

    Family and domain databases

    Gene3Di2.60.120.180. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR001137. Glyco_hydro_11.
    IPR013319. Glyco_hydro_11/12.
    IPR018208. Glyco_hydro_11_AS.
    [Graphical view]
    PfamiPF00457. Glyco_hydro_11. 1 hit.
    [Graphical view]
    PRINTSiPR00911. GLHYDRLASE11.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
    PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P55332-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSFKSLLVL CCAALGAFAT PVGSEDLAAR EASLLERSTP SSTGWSNGYY    50
    YSFWTDGGGD VTYTNGAGGS YTVQWSNVGN FVGGKGWNPG STRTINYGGS 100
    FNPSGNGYLA VYGWTQNPLI EYYIVESYGT YNPGSGGQHR GTVYSDGATY 150
    DIYTATRYNA PSIEGTATFE QFWSVRQSKR TGGTVTTANH FNAWAALGMR 200
    LGTHNYQIVA TEGYQSSGSA SITVY 225
    Length:225
    Mass (Da):24,070
    Last modified:October 1, 1996 - v1
    Checksum:i670F2C79602C7FEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49892 Genomic DNA. Translation: CAA90073.1.
    DQ490490 mRNA. Translation: ABF50866.1.
    AACD01000061 Genomic DNA. Translation: EAA59821.1.
    BN001302 Genomic DNA. Translation: CBF75776.1.
    PIRiS57477.
    RefSeqiXP_661217.1. XM_656125.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00005129; CADANIAP00005129; CADANIAG00005129.
    GeneIDi2873037.
    KEGGiani:AN3613.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49892 Genomic DNA. Translation: CAA90073.1 .
    DQ490490 mRNA. Translation: ABF50866.1 .
    AACD01000061 Genomic DNA. Translation: EAA59821.1 .
    BN001302 Genomic DNA. Translation: CBF75776.1 .
    PIRi S57477.
    RefSeqi XP_661217.1. XM_656125.1.

    3D structure databases

    ProteinModelPortali P55332.
    SMRi P55332. Positions 39-224.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH11. Glycoside Hydrolase Family 11.
    mycoCLAPi XYN11A_EMENI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00005129 ; CADANIAP00005129 ; CADANIAG00005129 .
    GeneIDi 2873037.
    KEGGi ani:AN3613.2.

    Phylogenomic databases

    eggNOGi NOG05353.
    HOGENOMi HOG000179135.
    OMAi NETALHE.
    OrthoDBi EOG7VQJQX.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    Gene3Di 2.60.120.180. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR001137. Glyco_hydro_11.
    IPR013319. Glyco_hydro_11/12.
    IPR018208. Glyco_hydro_11_AS.
    [Graphical view ]
    Pfami PF00457. Glyco_hydro_11. 1 hit.
    [Graphical view ]
    PRINTSi PR00911. GLHYDRLASE11.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
    PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes."
      Perez-Gonzalez J.A., de Graaff L.H., Visser J., Ramon D.
      Appl. Environ. Microbiol. 62:2179-2182(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
      Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
      Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    5. "Opposite patterns of expression of two Aspergillus nidulans xylanase genes with respect to ambient pH."
      MacCabe A.P., Orejas M., Perez-Gonzalez J.A., Ramon D.
      J. Bacteriol. 180:1331-1333(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Aroma improving in microvinification processes by the use of a recombinant wine yeast strain expressing the Aspergillus nidulans xlnA gene."
      Ganga M.A., Pinaga F., Valles S., Ramon D., Querol A.
      Int. J. Food Microbiol. 47:171-178(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY.
    7. "Carbon catabolite repression of the Aspergillus nidulans xlnA gene."
      Orejas M., MacCabe A.P., Perez Gonzalez J.A., Kumar S., Ramon D.
      Mol. Microbiol. 31:177-184(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "CreA mediates repression of the regulatory gene xlnR which controls the production of xylanolytic enzymes in Aspergillus nidulans."
      Tamayo E.N., Villanueva A., Hasper A.A., de Graaff L.H., Ramon D., Orejas M.
      Fungal Genet. Biol. 45:984-993(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiXYNA_EMENI
    AccessioniPrimary (citable) accession number: P55332
    Secondary accession number(s): C8V464
    , Q00173, Q1HFT4, Q5B767
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3