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Protein

Endo-1,4-beta-xylanase A

Gene

xlnA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

pH dependencei

Optimum pH is 5.4.1 Publication

Temperature dependencei

Optimum temperature is 52 degrees Celsius.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei121 – 1211NucleophilePROSITE-ProRule annotation
Active sitei212 – 2121Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. endo-1,4-beta-xylanase activity Source: UniProtKB

GO - Biological processi

  1. xylan catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiGH11. Glycoside Hydrolase Family 11.
mycoCLAPiXYN11A_EMENI.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
22 kDa xylanase
Xylanase X22
Gene namesi
Name:xlnA
ORF Names:AN3613
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome II

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

An increase in fruity aroma was organoleptically detected in the wine produced by the recombinant wine yeast strain T73 expressing xnlA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 225206Endo-1,4-beta-xylanase APRO_0000008004Add
BLAST

Expressioni

Inductioni

Expressed in the presence of D-xylose under conditions of alkaline ambient pH, probably under the regulation of the pacC transcription factor. Repressed in presence of glucose through the action of the creA transcription repressor.3 Publications

Structurei

3D structure databases

ProteinModelPortaliP55332.
SMRiP55332. Positions 39-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG05353.
HOGENOMiHOG000179135.
InParanoidiP55332.
KOiK01181.
OMAiYDILQTT.
OrthoDBiEOG7VQJQX.

Family and domain databases

Gene3Di2.60.120.180. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view]
PfamiPF00457. Glyco_hydro_11. 1 hit.
[Graphical view]
PRINTSiPR00911. GLHYDRLASE11.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSFKSLLVL CCAALGAFAT PVGSEDLAAR EASLLERSTP SSTGWSNGYY
60 70 80 90 100
YSFWTDGGGD VTYTNGAGGS YTVQWSNVGN FVGGKGWNPG STRTINYGGS
110 120 130 140 150
FNPSGNGYLA VYGWTQNPLI EYYIVESYGT YNPGSGGQHR GTVYSDGATY
160 170 180 190 200
DIYTATRYNA PSIEGTATFE QFWSVRQSKR TGGTVTTANH FNAWAALGMR
210 220
LGTHNYQIVA TEGYQSSGSA SITVY
Length:225
Mass (Da):24,070
Last modified:October 1, 1996 - v1
Checksum:i670F2C79602C7FEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49892 Genomic DNA. Translation: CAA90073.1.
DQ490490 mRNA. Translation: ABF50866.1.
AACD01000061 Genomic DNA. Translation: EAA59821.1.
BN001302 Genomic DNA. Translation: CBF75776.1.
PIRiS57477.
RefSeqiXP_661217.1. XM_656125.1.

Genome annotation databases

EnsemblFungiiCADANIAT00005129; CADANIAP00005129; CADANIAG00005129.
GeneIDi2873037.
KEGGiani:AN3613.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49892 Genomic DNA. Translation: CAA90073.1.
DQ490490 mRNA. Translation: ABF50866.1.
AACD01000061 Genomic DNA. Translation: EAA59821.1.
BN001302 Genomic DNA. Translation: CBF75776.1.
PIRiS57477.
RefSeqiXP_661217.1. XM_656125.1.

3D structure databases

ProteinModelPortaliP55332.
SMRiP55332. Positions 39-224.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH11. Glycoside Hydrolase Family 11.
mycoCLAPiXYN11A_EMENI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00005129; CADANIAP00005129; CADANIAG00005129.
GeneIDi2873037.
KEGGiani:AN3613.2.

Phylogenomic databases

eggNOGiNOG05353.
HOGENOMiHOG000179135.
InParanoidiP55332.
KOiK01181.
OMAiYDILQTT.
OrthoDBiEOG7VQJQX.

Enzyme and pathway databases

UniPathwayiUPA00114.

Family and domain databases

Gene3Di2.60.120.180. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view]
PfamiPF00457. Glyco_hydro_11. 1 hit.
[Graphical view]
PRINTSiPR00911. GLHYDRLASE11.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes."
    Perez-Gonzalez J.A., de Graaff L.H., Visser J., Ramon D.
    Appl. Environ. Microbiol. 62:2179-2182(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
    Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  5. "Opposite patterns of expression of two Aspergillus nidulans xylanase genes with respect to ambient pH."
    MacCabe A.P., Orejas M., Perez-Gonzalez J.A., Ramon D.
    J. Bacteriol. 180:1331-1333(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Aroma improving in microvinification processes by the use of a recombinant wine yeast strain expressing the Aspergillus nidulans xlnA gene."
    Ganga M.A., Pinaga F., Valles S., Ramon D., Querol A.
    Int. J. Food Microbiol. 47:171-178(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY.
  7. "Carbon catabolite repression of the Aspergillus nidulans xlnA gene."
    Orejas M., MacCabe A.P., Perez Gonzalez J.A., Kumar S., Ramon D.
    Mol. Microbiol. 31:177-184(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "CreA mediates repression of the regulatory gene xlnR which controls the production of xylanolytic enzymes in Aspergillus nidulans."
    Tamayo E.N., Villanueva A., Hasper A.A., de Graaff L.H., Ramon D., Orejas M.
    Fungal Genet. Biol. 45:984-993(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiXYNA_EMENI
AccessioniPrimary (citable) accession number: P55332
Secondary accession number(s): C8V464
, Q00173, Q1HFT4, Q5B767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.