P55327 (TPD52_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor protein D52

Alternative name(s):
Protein N8

Gene names

Name:

TPD52

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Forms a homodimer or heterodimer with other members of the family.
Tissue specificity	Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary. Ref.2
Developmental stage	Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney. Ref.2
Sequence similarities	Belongs to the TPD52 family.
Sequence caution	The sequence BAD97351.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell differentiation Inferred from expression pattern PubMed 15576473. Source: UniProtKB anatomical structure morphogenesis Traceable author statement Ref.2. Source: ProtInc secretion Traceable author statement PubMed 15576473. Source: UniProtKB
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 10942595. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay PubMed 16112108. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from direct assay PubMed 15576473. Source: UniProtKB protein heterodimerization activity Inferred from direct assay Ref.12. Source: UniProtKB protein homodimerization activity Inferred from direct assay Ref.12. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Tpd52	Q62393	2	EBI-782581,EBI-782591	From a different organism.
TPD52L1	Q16890	3	EBI-782581,EBI-717470

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P55327-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P55327-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: MDCREMDLYEDYQSPFDFDAGVNKSYLYLSPSGNSSPPGSPTLQKF → MDRGEQ

Isoform 3 (identifier: P55327-3)

Also known as: N8L;

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: MDCREMDLYE...PPGSPTLQKF → MTPRESAPGR...RAGDMDRGEQ

Isoform 4 (identifier: P55327-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: MDCREMDLYEDYQSPFDFDAGVNKSYLYLSPSGNSSPPGSPTLQKF → MDRGEQ
     169-169: K → KLQAFSHSFSIRSIQHSISMPAMR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Tumor protein D52		PRO_0000185738
Regions
	Coiled coil	62 – 114	53	Potential
Amino acid modifications
	Modified residue	171	1	Phosphoserine Ref.14
	Modified residue	173	1	Phosphothreonine Ref.14
	Modified residue	176	1	Phosphoserine Ref.15 Ref.17
	Modified residue	223	1	Phosphoserine Ref.14
Natural variations
	Alternative sequence	1 – 46	46	MDCRE…TLQKF → MDRGEQ in isoform 2 and isoform 4.		VSP_035751
	Alternative sequence	1 – 46	46	MDCRE…TLQKF → MTPRESAPGRGRAAPPRPTP LGVGTSRESPAEARRSSARR GGRSEPGRAAGGGAAEDTRR RAGDMDRGEQ in isoform 3.		VSP_037378
	Alternative sequence	169	1	K → KLQAFSHSFSIRSIQHSISM PAMR in isoform 4.		VSP_043510
	Natural variant	52	1	D → Y. Corresponds to variant rs35099105 [ dbSNP | Ensembl ].		VAR_061860
Experimental info
	Sequence conflict	74	1	E → K in BAD97351. Ref.8
	Sequence conflict	165	1	L → P in CAG46831. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 4C8183C5914BB976	FASTA	224	24,327
	10 20 30 40 50 60 MDCREMDLYE DYQSPFDFDA GVNKSYLYLS PSGNSSPPGS PTLQKFGLLR TDPVPEEGED 70 80 90 100 110 120 VAATISATET LSEEEQEELR RELAKVEEEI QTLSQVLAAK EKHLAEIKRK LGINSLQELK 130 140 150 160 170 180 QNIAKGWQDV TATSAYKKTS ETLSQAGQKA SAAFSSVGSV ITKKLEDVKN SPTFKSFEEK 190 200 210 220 VENLKSKVGG TKPAGGDFGE VLNSAANASA TTTEPLPEKT QESL
	Isoform 2 [UniParc]. Checksum: 4821EC86D1C3339D	FASTA	184	19,863
	10 20 30 40 50 60 MDRGEQGLLR TDPVPEEGED VAATISATET LSEEEQEELR RELAKVEEEI QTLSQVLAAK 70 80 90 100 110 120 EKHLAEIKRK LGINSLQELK QNIAKGWQDV TATSAYKKTS ETLSQAGQKA SAAFSSVGSV 130 140 150 160 170 180 ITKKLEDVKN SPTFKSFEEK VENLKSKVGG TKPAGGDFGE VLNSAANASA TTTEPLPEKT QESL
	Isoform 3 (N8L) [UniParc]. Checksum: B7D203C4941A02B2	FASTA	248	26,382
	10 20 30 40 50 60 MTPRESAPGR GRAAPPRPTP LGVGTSRESP AEARRSSARR GGRSEPGRAA GGGAAEDTRR 70 80 90 100 110 120 RAGDMDRGEQ GLLRTDPVPE EGEDVAATIS ATETLSEEEQ EELRRELAKV EEEIQTLSQV 130 140 150 160 170 180 LAAKEKHLAE IKRKLGINSL QELKQNIAKG WQDVTATSAY KKTSETLSQA GQKASAAFSS 190 200 210 220 230 240 VGSVITKKLE DVKNSPTFKS FEEKVENLKS KVGGTKPAGG DFGEVLNSAA NASATTTEPL PEKTQESL
	Isoform 4 [UniParc]. Checksum: 29027F1706989362	FASTA	207	22,477
	10 20 30 40 50 60 MDRGEQGLLR TDPVPEEGED VAATISATET LSEEEQEELR RELAKVEEEI QTLSQVLAAK 70 80 90 100 110 120 EKHLAEIKRK LGINSLQELK QNIAKGWQDV TATSAYKKTS ETLSQAGQKA SAAFSSVGSV 130 140 150 160 170 180 ITKKLEDVKL QAFSHSFSIR SIQHSISMPA MRNSPTFKSF EEKVENLKSK VGGTKPAGGD 190 200 FGEVLNSAAN ASATTTEPLP EKTQESL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A screening method to identify genes commonly overexpressed in carcinomas and the identification of a novel complementary DNA sequence." Byrne J.A., Tomasetto C., Garnier J.-M., Rouyer N., Mattei M.-G., Bellocq J.-P., Rio M.C., Basset P. Cancer Res. 55:2896-2903(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Mammary carcinoma.
[2]	"Isolation and characterization of a novel gene expressed in multiple cancers." Chen S.-L., Maroulakou I.G., Green J.E., Romano-Spica V., Modi W., Lautenberger J., Bhat N.K. Oncogene 12:741-751(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ALTERNATIVE SPLICING. Tissue: Lung tumor.
[3]	"PrLZ, a novel prostate-specific and androgen-responsive gene of the TPD52 family, amplified in chromosome 8q21.1 and overexpressed in human prostate cancer." Wang R., Xu J., Saramaki O., Visakorpi T., Sutherland W.M., Zhou J., Sen B., Lim S.D., Mabjeesh N., Amin M., Dong J.T., Petros J.A., Nelson P.S., Marshall F.F., Zhau H.E., Chung L.W. Cancer Res. 64:1589-1594(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4). Tissue: Colon, Hippocampus and Tongue.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Rectum tumor.
[8]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[9]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skin.
[12]	"Identification of homo- and heteromeric interactions between members of the breast carcinoma-associated D52 protein family using the yeast two-hybrid system." Byrne J.A., Nourse C.R., Basset P., Gunning P. Oncogene 16:873-881(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION.
[13]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; THR-173 AND SER-223, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18914 mRNA. Translation: AAC50183.1. S82081 mRNA. Translation: AAB36475.1. AF202897 mRNA. Translation: AAO22156.1. AK295468 mRNA. Translation: BAH12078.1. AK296615 mRNA. Translation: BAH12400.1. AK313135 mRNA. Translation: BAG35954.1. CR542034 mRNA. Translation: CAG46831.1. CR542064 mRNA. Translation: CAG46861.1. BT019444 mRNA. Translation: AAV38251.1. BX640835 mRNA. Translation: CAE45908.1. AK223631 mRNA. Translation: BAD97351.1. Different initiation. AC009686 Genomic DNA. No translation available. AC018952 Genomic DNA. No translation available. AC036214 Genomic DNA. No translation available. AC104212 Genomic DNA. No translation available. CH471068 Genomic DNA. Translation: EAW87074.1. BC018117 mRNA. Translation: AAH18117.1.
IPI	IPI00619958. IPI00873344. IPI00896474. IPI00941154.
PIR	I38910.
RefSeq	NP_001020423.1. NM_001025252.1. NP_001020424.1. NM_001025253.1. NP_005070.1. NM_005079.2.
UniGene	Hs.368433.
3D structure databases
ProteinModelPortal	P55327.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55327. 7 interactions.
MINT	MINT-5003652.
STRING	9606.ENSP00000368391.
PTM databases
PhosphoSite	P55327.
Polymorphism databases
DMDM	215273966.
Proteomic databases
PaxDb	P55327.
PRIDE	P55327.
Protocols and materials databases
DNASU	7163.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379096; ENSP00000368390; ENSG00000076554. ENST00000379097; ENSP00000368391; ENSG00000076554. ENST00000518937; ENSP00000429915; ENSG00000076554.
GeneID	7163.
KEGG	hsa:7163.
UCSC	uc003ybr.1. human. uc003ybt.1. human.
Organism-specific databases
CTD	7163.
GeneCards	GC08M080870.
H-InvDB	HIX0152680.
HGNC	HGNC:12005. TPD52.
HPA	HPA028427.
MIM	604068. gene.
neXtProt	NX_P55327.
PharmGKB	PA36686.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG251958.
HOVERGEN	HBG058643.
InParanoid	P55327.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
ArrayExpress	P55327.
Bgee	P55327.
CleanEx	HS_TPD52.
Genevestigator	P55327.
GermOnline	ENSG00000076554. Homo sapiens.
Family and domain databases
InterPro	IPR007327. TPD52. [Graphical view]
PANTHER	PTHR19307. PTHR19307. 1 hit.
Pfam	PF04201. TPD52. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TPD52. human.
GenomeRNAi	7163.
NextBio	28030.
SOURCE	Search...

Entry information

Entry name

TPD52_HUMAN

Accession

Primary (citable) accession number: P55327
Secondary accession number(s): B7Z414 , E5RKB4, Q13056, Q53EK8, Q6FGP3, Q6FGS3, Q86YZ2, Q9UCX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.