Reviewed, UniProtKB/Swiss-Prot P55320 (GIH_HOMAM)

Names and origin

Protein names	Recommended name:     Gonad-inhibiting hormone       Short name=GIH Alternative name(s):     Vitellogenesis-inhibiting hormone       Short name=VIH
Organism	Homarus americanus (American lobster)
Taxonomic identifier	6706 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Astacidea › Nephropoidea › Nephropidae › Homarus

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits vitellogenesis in female animals. Plays a prominent role in the regulation of reproduction/molting processes.
Subcellular location	Secreted.
Tissue specificity	Produced in the eyestalk X-organ sinus gland complex of male and female lobsters.
Sequence similarities	Belongs to the arthropod CHH/MIH/GIH/VIH hormone family.
Mass spectrometry	Molecular mass is 9135 Da from positions 32 - 109. Determined by FAB. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone Neuropeptide
PTM	Amidation Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	neuropeptide hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.2
	Peptide	32 – 109	78	Gonad-inhibiting hormone		PRO_0000019075
Amino acid modifications
	Modified residue	109	1	Alanine amide Potential
	Disulfide bond	41 ↔ 78		By similarity
	Disulfide bond	58 ↔ 74		By similarity
	Disulfide bond	61 ↔ 87		By similarity
Experimental info
	Sequence conflict	83	1	D → W AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55320-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8C1955EF737747F6	FASTA	112	12,841
	10 20 30 40 50 60 MVTRVGSGFS VQRVWLLLVI VVVLCGSVTQ QASAWFTNDE CPGVMGNRDL YEKVAWVCND 70 80 90 100 110 CANIFRNNDV GVMCKKDCFH TMDFLWCVYA TERHGEIDQF RKWVSILRAG RK

References

[1]	"Cloning and expression of mRNA encoding prepro-gonad-inhibiting hormone (GIH) in the lobster Homarus americanus." de Kleijn D.P.V., Sleutels F.J.G.T., Martens G.J.M., van Herp F. FEBS Lett. 353:255-258(1994) [PubMed: 7957869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Eyestalk.
[2]	"Primary structure of two isoforms of the vitellogenesis inhibiting hormone from the lobster Homarus americanus." Soyez D., Le Caer J.-P., Noel P.Y., Rossier J. Neuropeptides 20:25-32(1991) [PubMed: 1791922] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-108, MASS SPECTROMETRY. Tissue: Sinus gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X87192 mRNA. Translation: CAA60644.1.
PIR	A45586. S48747.
3D structure databases
HSSP	HSSP built from PDB template 1J0T based on UniProtKB P55847.
ModBase	Search...
Family and domain databases
InterPro	IPR001166. Crust_neurhormone. IPR018251. Crust_neurhormone_CS. IPR001262. Crust_neurhormone_MIH/MOIH/GIH. [Graphical view]
Gene3D	G3DSA:1.10.2010.10. CHH_MIH_GIH. 1 hit.
Pfam	PF01147. Crust_neurohorm. 1 hit. [Graphical view]
PRINTS	PR00549. HYPRGLYCEMC2. PR00550. HYPRGLYCEMIC.
PROSITE	PS01250. CHH_MIH_GIH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GIH_HOMAM

Accession

Primary (citable) accession number: P55320
Secondary accession number(s): Q25013

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)