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Protein

Hepatocyte nuclear factor 3-gamma

Gene

FOXA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi116 – 207Fork-headPROSITE-ProRule annotationAdd BLAST92

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170608-MONOMER.
ReactomeiR-HSA-210745. Regulation of gene expression in beta cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 3-gamma
Short name:
HNF-3-gamma
Short name:
HNF-3G
Alternative name(s):
Fork head-related protein FKH H3
Forkhead box protein A3
Transcription factor 3G
Short name:
TCF-3G
Gene namesi
Name:FOXA3
Synonyms:HNF3G, TCF3G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:5023. FOXA3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3171.
OpenTargetsiENSG00000170608.
PharmGKBiPA201092.

Polymorphism and mutation databases

BioMutaiFOXA3.
DMDMi8247938.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000918001 – 350Hepatocyte nuclear factor 3-gammaAdd BLAST350

Proteomic databases

EPDiP55318.
MaxQBiP55318.
PaxDbiP55318.
PeptideAtlasiP55318.
PRIDEiP55318.

PTM databases

iPTMnetiP55318.
PhosphoSitePlusiP55318.

Expressioni

Tissue specificityi

Expressed in erythroleukemia and hepatoma cell lines and in liver and pancreas. Not expressed in any other cell lines or tissues examined.1 Publication

Developmental stagei

Detected in prenatal liver nuclear extracts (12.4-27 weeks estimated gestational age). Not detected in postnatal liver samples.1 Publication

Gene expression databases

BgeeiENSG00000170608.
CleanExiHS_FOXA3.
ExpressionAtlasiP55318. baseline and differential.
GenevisibleiP55318. HS.

Organism-specific databases

HPAiCAB015154.
HPA054034.

Interactioni

Subunit structurei

Interacts with FOXA2.2 Publications

Protein-protein interaction databases

BioGridi109413. 50 interactors.
IntActiP55318. 43 interactors.
STRINGi9606.ENSP00000304004.

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi122 – 131Combined sources10
Helixi140 – 150Combined sources11
Helixi152 – 155Combined sources4
Helixi158 – 171Combined sources14
Beta strandi175 – 178Combined sources4
Beta strandi190 – 193Combined sources4
Helixi195 – 197Combined sources3
Beta strandi206 – 208Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VTNX-ray2.50C115-215[»]
ProteinModelPortaliP55318.
SMRiP55318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3563. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00760000118904.
HOGENOMiHOG000231817.
HOVERGENiHBG006621.
InParanoidiP55318.
KOiK08038.
OMAiVYYQGVY.
OrthoDBiEOG091G0HW9.
PhylomeDBiP55318.
TreeFamiTF316127.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013638. Fork-head_N.
IPR001766. Fork_head_dom.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
PF08430. Forkhead_N. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGSVKMEAH DLAEWSYYPE AGEVYSPVTP VPTMAPLNSY MTLNPLSSPY
60 70 80 90 100
PPGGLPASPL PSGPLAPPAP AAPLGPTFPG LGVSGGSSSS GYGAPGPGLV
110 120 130 140 150
HGKEMPKGYR RPLAHAKPPY SYISLITMAI QQAPGKMLTL SEIYQWIMDL
160 170 180 190 200
FPYYRENQQR WQNSIRHSLS FNDCFVKVAR SPDKPGKGSY WALHPSSGNM
210 220 230 240 250
FENGCYLRRQ KRFKLEEKVK KGGSGAATTT RNGTGSAAST TTPAATVTSP
260 270 280 290 300
PQPPPPAPEP EAQGGEDVGA LDCGSPASST PYFTGLELPG ELKLDAPYNF
310 320 330 340 350
NHPFSINNLM SEQTPAPPKL DVGFGGYGAE GGEPGVYYQG LYSRSLLNAS
Length:350
Mass (Da):37,140
Last modified:May 30, 2000 - v2
Checksum:i07657D41B0867101
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52Missing in AAA58477 (PubMed:8499623).Curated1
Sequence conflicti83V → L in AAA58477 (PubMed:8499623).Curated1
Sequence conflicti107K → M in BAD97193 (Ref. 4) Curated1
Sequence conflicti111 – 113RPL → AP in AAA58477 (PubMed:8499623).Curated3
Sequence conflicti137M → V in AAA58477 (PubMed:8499623).Curated1
Sequence conflicti156E → D in AAA58477 (PubMed:8499623).Curated1
Sequence conflicti227 – 228AT → S in AAA58477 (PubMed:8499623).Curated2
Sequence conflicti291E → D in AAA58477 (PubMed:8499623).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00885991G → R.Corresponds to variant rs758330593dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12141 mRNA. Translation: AAA58477.1.
AF176114, AF176113 Genomic DNA. Translation: AAD51980.1.
BT006720 mRNA. Translation: AAP35366.1.
AK223473 mRNA. Translation: BAD97193.1.
EU275778 Genomic DNA. Translation: ABX44664.1.
CH471126 Genomic DNA. Translation: EAW57394.1.
BC016024 mRNA. Translation: AAH16024.1.
CCDSiCCDS12677.1.
PIRiC48924.
RefSeqiNP_004488.2. NM_004497.2.
UniGeneiHs.36137.

Genome annotation databases

EnsembliENST00000302177; ENSP00000304004; ENSG00000170608.
GeneIDi3171.
KEGGihsa:3171.
UCSCiuc002pdr.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Hepatocyte nuclear factors entry

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12141 mRNA. Translation: AAA58477.1.
AF176114, AF176113 Genomic DNA. Translation: AAD51980.1.
BT006720 mRNA. Translation: AAP35366.1.
AK223473 mRNA. Translation: BAD97193.1.
EU275778 Genomic DNA. Translation: ABX44664.1.
CH471126 Genomic DNA. Translation: EAW57394.1.
BC016024 mRNA. Translation: AAH16024.1.
CCDSiCCDS12677.1.
PIRiC48924.
RefSeqiNP_004488.2. NM_004497.2.
UniGeneiHs.36137.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VTNX-ray2.50C115-215[»]
ProteinModelPortaliP55318.
SMRiP55318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109413. 50 interactors.
IntActiP55318. 43 interactors.
STRINGi9606.ENSP00000304004.

PTM databases

iPTMnetiP55318.
PhosphoSitePlusiP55318.

Polymorphism and mutation databases

BioMutaiFOXA3.
DMDMi8247938.

Proteomic databases

EPDiP55318.
MaxQBiP55318.
PaxDbiP55318.
PeptideAtlasiP55318.
PRIDEiP55318.

Protocols and materials databases

DNASUi3171.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302177; ENSP00000304004; ENSG00000170608.
GeneIDi3171.
KEGGihsa:3171.
UCSCiuc002pdr.3. human.

Organism-specific databases

CTDi3171.
DisGeNETi3171.
GeneCardsiFOXA3.
HGNCiHGNC:5023. FOXA3.
HPAiCAB015154.
HPA054034.
MIMi602295. gene.
neXtProtiNX_P55318.
OpenTargetsiENSG00000170608.
PharmGKBiPA201092.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3563. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00760000118904.
HOGENOMiHOG000231817.
HOVERGENiHBG006621.
InParanoidiP55318.
KOiK08038.
OMAiVYYQGVY.
OrthoDBiEOG091G0HW9.
PhylomeDBiP55318.
TreeFamiTF316127.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170608-MONOMER.
ReactomeiR-HSA-210745. Regulation of gene expression in beta cells.

Miscellaneous databases

GeneWikiiFOXA3.
GenomeRNAii3171.
PROiP55318.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170608.
CleanExiHS_FOXA3.
ExpressionAtlasiP55318. baseline and differential.
GenevisibleiP55318. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013638. Fork-head_N.
IPR001766. Fork_head_dom.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
PF08430. Forkhead_N. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOXA3_HUMAN
AccessioniPrimary (citable) accession number: P55318
Secondary accession number(s): A9LYI5, Q53F16, Q9UMW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.