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P55318

- FOXA3_HUMAN

UniProt

P55318 - FOXA3_HUMAN

Protein

Hepatocyte nuclear factor 3-gamma

Gene

FOXA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites By similarity. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi116 – 20792Fork-headPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: Ensembl
    2. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    3. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. cellular glucose homeostasis Source: UniProtKB
    3. cellular response to starvation Source: Ensembl
    4. chromatin modification Source: UniProtKB-KW
    5. endocrine pancreas development Source: Reactome
    6. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    7. spermatogenesis Source: UniProtKB-KW
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Developmental protein

    Keywords - Biological processi

    Differentiation, Spermatogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_13819. Regulation of gene expression in beta cells.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hepatocyte nuclear factor 3-gamma
    Short name:
    HNF-3-gamma
    Short name:
    HNF-3G
    Alternative name(s):
    Fork head-related protein FKH H3
    Forkhead box protein A3
    Transcription factor 3G
    Short name:
    TCF-3G
    Gene namesi
    Name:FOXA3
    Synonyms:HNF3G, TCF3G
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:5023. FOXA3.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA201092.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350Hepatocyte nuclear factor 3-gammaPRO_0000091800Add
    BLAST

    Proteomic databases

    MaxQBiP55318.
    PaxDbiP55318.
    PRIDEiP55318.

    PTM databases

    PhosphoSiteiP55318.

    Expressioni

    Tissue specificityi

    Expressed in erythroleukemia and hepatoma cell lines and in liver and pancreas. Not expressed in any other cell lines or tissues examined.1 Publication

    Developmental stagei

    Detected in prenatal liver nuclear extracts (12.4-27 weeks estimated gestational age). Not detected in postnatal liver samples.1 Publication

    Gene expression databases

    ArrayExpressiP55318.
    BgeeiP55318.
    CleanExiHS_FOXA3.
    GenevestigatoriP55318.

    Organism-specific databases

    HPAiCAB015154.

    Interactioni

    Subunit structurei

    Interacts with FOXA2.2 Publications

    Protein-protein interaction databases

    BioGridi109413. 4 interactions.
    IntActiP55318. 1 interaction.
    STRINGi9606.ENSP00000304004.

    Structurei

    Secondary structure

    1
    350
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi122 – 13110
    Helixi140 – 15011
    Helixi152 – 1554
    Helixi158 – 17114
    Beta strandi175 – 1784
    Beta strandi190 – 1934
    Helixi195 – 1973
    Beta strandi206 – 2083

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VTNX-ray2.50C115-215[»]
    ProteinModelPortaliP55318.
    SMRiP55318. Positions 115-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5025.
    HOGENOMiHOG000231817.
    HOVERGENiHBG006621.
    InParanoidiP55318.
    KOiK08038.
    OMAiVYYQGVY.
    PhylomeDBiP55318.
    TreeFamiTF316127.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR013638. Fork-head_N.
    IPR018533. Forkhead_box_C.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00250. Fork_head. 1 hit.
    PF08430. Fork_head_N. 1 hit.
    PF09354. HNF_C. 1 hit.
    [Graphical view]
    PRINTSiPR00053. FORKHEAD.
    SMARTiSM00339. FH. 1 hit.
    [Graphical view]
    PROSITEiPS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55318-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGSVKMEAH DLAEWSYYPE AGEVYSPVTP VPTMAPLNSY MTLNPLSSPY    50
    PPGGLPASPL PSGPLAPPAP AAPLGPTFPG LGVSGGSSSS GYGAPGPGLV 100
    HGKEMPKGYR RPLAHAKPPY SYISLITMAI QQAPGKMLTL SEIYQWIMDL 150
    FPYYRENQQR WQNSIRHSLS FNDCFVKVAR SPDKPGKGSY WALHPSSGNM 200
    FENGCYLRRQ KRFKLEEKVK KGGSGAATTT RNGTGSAAST TTPAATVTSP 250
    PQPPPPAPEP EAQGGEDVGA LDCGSPASST PYFTGLELPG ELKLDAPYNF 300
    NHPFSINNLM SEQTPAPPKL DVGFGGYGAE GGEPGVYYQG LYSRSLLNAS 350
    Length:350
    Mass (Da):37,140
    Last modified:May 30, 2000 - v2
    Checksum:i07657D41B0867101
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521Missing in AAA58477. (PubMed:8499623)Curated
    Sequence conflicti83 – 831V → L in AAA58477. (PubMed:8499623)Curated
    Sequence conflicti107 – 1071K → M in BAD97193. 1 PublicationCurated
    Sequence conflicti111 – 1133RPL → AP in AAA58477. (PubMed:8499623)Curated
    Sequence conflicti137 – 1371M → V in AAA58477. (PubMed:8499623)Curated
    Sequence conflicti156 – 1561E → D in AAA58477. (PubMed:8499623)Curated
    Sequence conflicti227 – 2282AT → S in AAA58477. (PubMed:8499623)Curated
    Sequence conflicti291 – 2911E → D in AAA58477. (PubMed:8499623)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti91 – 911G → R.
    VAR_008859

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12141 mRNA. Translation: AAA58477.1.
    AF176114, AF176113 Genomic DNA. Translation: AAD51980.1.
    BT006720 mRNA. Translation: AAP35366.1.
    AK223473 mRNA. Translation: BAD97193.1.
    EU275778 Genomic DNA. Translation: ABX44664.1.
    CH471126 Genomic DNA. Translation: EAW57394.1.
    BC016024 mRNA. Translation: AAH16024.1.
    CCDSiCCDS12677.1.
    PIRiC48924.
    RefSeqiNP_004488.2. NM_004497.2.
    UniGeneiHs.36137.

    Genome annotation databases

    EnsembliENST00000302177; ENSP00000304004; ENSG00000170608.
    GeneIDi3171.
    KEGGihsa:3171.
    UCSCiuc002pdr.3. human.

    Polymorphism databases

    DMDMi8247938.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Hepatocyte nuclear factors entry

    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12141 mRNA. Translation: AAA58477.1 .
    AF176114 , AF176113 Genomic DNA. Translation: AAD51980.1 .
    BT006720 mRNA. Translation: AAP35366.1 .
    AK223473 mRNA. Translation: BAD97193.1 .
    EU275778 Genomic DNA. Translation: ABX44664.1 .
    CH471126 Genomic DNA. Translation: EAW57394.1 .
    BC016024 mRNA. Translation: AAH16024.1 .
    CCDSi CCDS12677.1.
    PIRi C48924.
    RefSeqi NP_004488.2. NM_004497.2.
    UniGenei Hs.36137.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VTN X-ray 2.50 C 115-215 [» ]
    ProteinModelPortali P55318.
    SMRi P55318. Positions 115-215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109413. 4 interactions.
    IntActi P55318. 1 interaction.
    STRINGi 9606.ENSP00000304004.

    PTM databases

    PhosphoSitei P55318.

    Polymorphism databases

    DMDMi 8247938.

    Proteomic databases

    MaxQBi P55318.
    PaxDbi P55318.
    PRIDEi P55318.

    Protocols and materials databases

    DNASUi 3171.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000302177 ; ENSP00000304004 ; ENSG00000170608 .
    GeneIDi 3171.
    KEGGi hsa:3171.
    UCSCi uc002pdr.3. human.

    Organism-specific databases

    CTDi 3171.
    GeneCardsi GC19P046368.
    HGNCi HGNC:5023. FOXA3.
    HPAi CAB015154.
    MIMi 602295. gene.
    neXtProti NX_P55318.
    PharmGKBi PA201092.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5025.
    HOGENOMi HOG000231817.
    HOVERGENi HBG006621.
    InParanoidi P55318.
    KOi K08038.
    OMAi VYYQGVY.
    PhylomeDBi P55318.
    TreeFami TF316127.

    Enzyme and pathway databases

    Reactomei REACT_13819. Regulation of gene expression in beta cells.

    Miscellaneous databases

    GeneWikii FOXA3.
    GenomeRNAii 3171.
    NextBioi 12574.
    PROi P55318.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P55318.
    Bgeei P55318.
    CleanExi HS_FOXA3.
    Genevestigatori P55318.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR013638. Fork-head_N.
    IPR018533. Forkhead_box_C.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00250. Fork_head. 1 hit.
    PF08430. Fork_head_N. 1 hit.
    PF09354. HNF_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00053. FORKHEAD.
    SMARTi SM00339. FH. 1 hit.
    [Graphical view ]
    PROSITEi PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells."
      Hromas R., Moore J., Johnston T., Socha C., Klemsz M.
      Blood 81:2854-2859(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "The human HNF-3 genes: cloning, partial sequence and mutation screening in patients with impaired glucose homeostasis."
      Navas M.A., Vaisse C., Boger S., Heimesaat M., Kollee L.A., Stoffel M.
      Hum. Hered. 50:370-381(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Gastric mucosa.
    5. SeattleSNPs variation discovery resource
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Uterus.
    8. "The role of HNF1alpha, HNF3gamma, and cyclic AMP in glucose-6-phosphatase gene activation."
      Lin B., Morris D.W., Chou J.Y.
      Biochemistry 36:14096-14106(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROMOTER BINDING.
    9. "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte nuclear factor-3 gamma."
      Rodriguez-Antona C., Bort R., Jover R., Tindberg N., Ingelman-Sundberg M., Gomez-Lechon M.J., Castell J.V.
      Mol. Pharmacol. 63:1180-1189(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Differential binding and co-binding pattern of FOXA1 and FOXA3 and their relation to H3K4me3 in HepG2 cells revealed by ChIP-seq."
      Motallebipour M., Ameur A., Reddy Bysani M.S., Patra K., Wallerman O., Mangion J., Barker M.A., McKernan K.J., Komorowski J., Wadelius C.
      Genome Biol. 10:R129.0-R129.0(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FOXA2.
    11. "Regulation of the CYP3A4 and CYP3A7 promoters by members of the nuclear factor I transcription factor family."
      Riffel A.K., Schuenemann E., Vyhlidal C.A.
      Mol. Pharmacol. 76:1104-1114(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROMOTER-BINDING, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    12. "Co-crystal structure of the HNF-3/fork head DNA-recognition motif resembles histone H5."
      Clark K.L., Halay E.D., Lai E., Burley S.K.
      Nature 364:412-420(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 115-215 IN COMPLEX WITH DNA.

    Entry informationi

    Entry nameiFOXA3_HUMAN
    AccessioniPrimary (citable) accession number: P55318
    Secondary accession number(s): A9LYI5, Q53F16, Q9UMW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3