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Protein

Complement component C8 beta chain

Gene

C8b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.By similarity

GO - Biological processi

Keywordsi

Biological processComplement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-RNO-166665 Terminal pathway of complement
R-RNO-977606 Regulation of Complement cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C8 beta chain
Alternative name(s):
Complement component 8 subunit beta
Gene namesi
Name:C8b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi2239 C8b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane attack complex, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
PropeptideiPRO_000029130532 – 53By similarityAdd BLAST22
ChainiPRO_000016250954 – 589Complement component C8 beta chainAdd BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi64 ↔ 99By similarity
Glycosylationi69C-linked (Man) tryptophanBy similarity1
Glycosylationi72C-linked (Man) tryptophanBy similarity1
Disulfide bondi75 ↔ 78By similarity
Disulfide bondi109 ↔ 115By similarity
Disulfide bondi121 ↔ 132By similarity
Disulfide bondi126 ↔ 145By similarity
Disulfide bondi139 ↔ 154By similarity
Glycosylationi242N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei417PhosphothreonineBy similarity1
Disulfide bondi502 ↔ 549By similarity
Disulfide bondi504 ↔ 520By similarity
Disulfide bondi507 ↔ 522By similarity
Disulfide bondi524 ↔ 533By similarity
Glycosylationi550C-linked (Man) tryptophanBy similarity1
Glycosylationi553C-linked (Man) tryptophanBy similarity1
Disulfide bondi556 ↔ 589By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP55314
PRIDEiP55314

Expressioni

Gene expression databases

BgeeiENSRNOG00000007639
GenevisibleiP55314 RN

Interactioni

Subunit structurei

Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b sequentially binds C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010100

Structurei

3D structure databases

ProteinModelPortaliP55314
SMRiP55314
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 116TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini120 – 155LDL-receptor class APROSITE-ProRule annotationAdd BLAST36
Domaini157 – 503MACPFPROSITE-ProRule annotationAdd BLAST347
Domaini504 – 534EGF-likeAdd BLAST31
Domaini544 – 587TSP type-1 2PROSITE-ProRule annotationAdd BLAST44

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE7H Eukaryota
ENOG410Y2J1 LUCA
GeneTreeiENSGT00550000074478
HOGENOMiHOG000231146
HOVERGENiHBG106489
InParanoidiP55314
KOiK03998
OMAiLMLHYEF
OrthoDBiEOG091G07LK
PhylomeDBiP55314
TreeFamiTF330498

Family and domain databases

CDDicd00112 LDLa, 1 hit
Gene3Di2.20.100.10, 2 hits
InterProiView protein in InterPro
IPR037566 Complement_C8_beta
IPR013032 EGF-like_CS
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR001862 MAC_perforin
IPR020864 MACPF
IPR020863 MACPF_CS
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PANTHERiPTHR19325:SF401 PTHR19325:SF401, 1 hit
PfamiView protein in Pfam
PF00057 Ldl_recept_a, 1 hit
PF01823 MACPF, 1 hit
PF00090 TSP_1, 2 hits
PRINTSiPR00764 COMPLEMENTC9
SMARTiView protein in SMART
SM00192 LDLa, 1 hit
SM00457 MACPF, 1 hit
SM00209 TSP1, 2 hits
SUPFAMiSSF57424 SSF57424, 1 hit
SSF82895 SSF82895, 2 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
PS00279 MACPF_1, 1 hit
PS51412 MACPF_2, 1 hit
PS50092 TSP1, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTGAQVWRA LAKSCLLCAA LGCLHLPGAR GEKPDFFETN AVNGSLVRSR
60 70 80 90 100
PVRSVDVTPA PTDCQLSTWS SWTACDPCQK KRYRHTYLLR PSQFYGELCD
110 120 130 140 150
FSDKEVEDCV TNRACRSQVR CEGFVCAQTG RCVNRRLLCN GDNDCGDQSD
160 170 180 190 200
EANCRRIYKK CSQDMEQYWA IGNLASGINL FTNTFEGPVL DHRYYAGACS
210 220 230 240 250
PHYILNTNFR KPYNVESYTP QTQGKYEFAL TEYESYFDFE HNVTEKATSK
260 270 280 290 300
SSFKFGFKLD GLVEFGVRKE SNEGRHYISR TKRFSHTKSK FLHARSVLEV
310 320 330 340 350
AHYKLKSRQL MLHYEFLQRV KSLPLEYSYG EYRDLLRDFG THFITEAVLG
360 370 380 390 400
GIYEYTLIMN KDAMERGDYT LDHVSACAGG GFQIGGNVYK VYLKLGVSEK
410 420 430 440 450
KCSDILNEIK DRNKRRTMVE DLVVLVRGGT SEYITSLAYK DLPTAELMKE
460 470 480 490 500
WGDAVQYNPA IIKLKAEPLY ELVTATDFAY SSTVKQNMKK ALEEFQMEVS
510 520 530 540 550
SCRCAPCRNN GVPILKESRC ECICPAGFQG VACEVTNRKD IPIDGKWSCW
560 570 580
SDWSPCSGGR KTRQRQCNNP APQRGGSPCS GPASETLDC
Length:589
Mass (Da):66,667
Last modified:June 12, 2007 - v2
Checksum:i1F0143FE1BFA2EB3
GO

Sequence cautioni

The sequence CO561829 differs from that shown. Reason: Frameshift at positions 248 and 256.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti451W → R in AAA82890 (PubMed:7665162).Curated1
Sequence conflicti458N → I in AAA82890 (PubMed:7665162).Curated1
Sequence conflicti464L → S in AAA82890 (PubMed:7665162).Curated1
Sequence conflicti497M → K in AAA82890 (PubMed:7665162).Curated1
Sequence conflicti526 – 528AGF → VGL in AAA82890 (PubMed:7665162).Curated3
Sequence conflicti588D → N in AAA82890 (PubMed:7665162).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03041555 Genomic DNA No translation available.
AABR03042213 Genomic DNA No translation available.
CO561829 mRNA No translation available.
BC090023 mRNA Translation: AAH90023.1
U20194 mRNA Translation: AAA82890.1
RefSeqiNP_001178688.1, NM_001191759.1
XP_006238557.1, XM_006238495.3
UniGeneiRn.110603

Genome annotation databases

EnsembliENSRNOT00000010100; ENSRNOP00000010100; ENSRNOG00000007639
GeneIDi313421
KEGGirno:313421
UCSCiRGD:2239 rat

Entry informationi

Entry nameiCO8B_RAT
AccessioniPrimary (citable) accession number: P55314
Secondary accession number(s): Q5EB58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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