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Protein

Alpha-2-macroglobulin receptor-associated protein

Gene

Lrpap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name:
Alpha-2-MRAP
Alternative name(s):
Heparin-binding protein 44
Short name:
HBP-44
Low density lipoprotein receptor-related protein-associated protein 1
Short name:
RAP
Gene namesi
Name:Lrpap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96829 Lrpap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000002072529 – 360Alpha-2-macroglobulin receptor-associated proteinAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Modified residuei138PhosphoserineCombined sources1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP55302
PaxDbiP55302
PeptideAtlasiP55302
PRIDEiP55302

2D gel databases

REPRODUCTION-2DPAGEiIPI00469307
P55302

PTM databases

iPTMnetiP55302
PhosphoSitePlusiP55302

Expressioni

Tissue specificityi

Highly expressed in PYS-2 parietal endoderm cells and in the kidney. The RNA level increased about 10-fold during differentiation of F9 embryonal carcinoma cells to parietal endoderm cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000029103
ExpressionAtlasiP55302 baseline and differential
GenevisibleiP55302 MM

Interactioni

Subunit structurei

Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor. Interacts with LRP2/glycoprotein 330. Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2012052 interactors.
IntActiP55302 5 interactors.
MINTiP55302
STRINGi10090.ENSMUSP00000030986

Structurei

3D structure databases

ProteinModelPortaliP55302
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 356LDL receptor bindingSequence analysisAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili184 – 302Sequence analysisAdd BLAST119

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 360Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the alpha-2-MRAP family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG3956 Eukaryota
ENOG410YJFP LUCA
GeneTreeiENSGT00390000004855
HOVERGENiHBG000197
InParanoidiP55302
KOiK22290
OMAiNQVWEKA
OrthoDBiEOG091G0EO4
PhylomeDBiP55302
TreeFamiTF320678

Family and domain databases

CDDicd14806 RAP_D1, 1 hit
cd14807 RAP_D2, 1 hit
cd14808 RAP_D3, 1 hit
Gene3Di1.20.81.103 hits
InterProiView protein in InterPro
IPR038003 A2-macroglobuin_RAP
IPR010483 Alpha_2_MRAP_C
IPR009066 MG_RAP_rcpt_1
IPR038001 RAP_D2
IPR037999 RAP_D3
IPR036744 RAP_sf
PANTHERiPTHR16560 PTHR16560, 1 hit
PfamiView protein in Pfam
PF06401 Alpha-2-MRAP_C, 1 hit
PF06400 Alpha-2-MRAP_N, 1 hit
SUPFAMiSSF47045 SSF47045, 3 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55302-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRRERVST LPRLQLLVLL LLPLMLVPQP IAGHGGKYSR EKNEPEMAAK
60 70 80 90 100
RESGEEFRME KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK
110 120 130 140 150
VEGLDKDGEK EAKLIHNLNV ILARYGLDGR KDAQMVHSNA LNEDTQDELG
160 170 180 190 200
DPRLEKLWHK AKTSGKFSSE ELDKLWREFL HYKEKIQEYN VLLDTLSRAE
210 220 230 240 250
EGYENLLSPS DMAHIKSDTL ISKHSELKDR LRSINQGLDR LRKVSHQGYG
260 270 280 290 300
STTEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ
310 320 330 340 350
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS
360
RVSRARHNEL
Length:360
Mass (Da):42,215
Last modified:October 1, 1996 - v1
Checksum:i72ED46FA0AC05D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67967 mRNA Translation: AAC60668.1
D00622 mRNA Translation: BAA00500.1
CCDSiCCDS19225.1
PIRiJX0281
RefSeqiNP_038615.2, NM_013587.2
UniGeneiMm.277661

Genome annotation databases

EnsembliENSMUST00000030986; ENSMUSP00000030986; ENSMUSG00000029103
GeneIDi16976
KEGGimmu:16976
UCSCiuc008xdo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiAMRP_MOUSE
AccessioniPrimary (citable) accession number: P55302
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome