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P55302 (AMRP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2-macroglobulin receptor-associated protein

Short name=Alpha-2-MRAP
Alternative name(s):
Heparin-binding protein 44
Short name=HBP-44
Low density lipoprotein receptor-related protein-associated protein 1
Short name=RAP
Gene names
Name:Lrpap1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

Subunit structure

Present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. Binds LRP1B; binding is followed by internalization and degradation By similarity.

Subcellular location

Endoplasmic reticulum By similarity. Cytoplasm By similarity. Cell surface By similarity. Note: Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum By similarity.

Tissue specificity

Highly expressed in PYS-2 parietal endoderm cells and in the kidney. The RNA level increased about 10-fold during differentiation of F9 embryonal carcinoma cells to parietal endoderm cells. Ref.2

Sequence similarities

Belongs to the alpha-2-MRAP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 360332Alpha-2-macroglobulin receptor-associated protein
PRO_0000020725

Regions

Region240 – 356117LDL receptor binding Potential
Coiled coil184 – 302119 Potential
Motif357 – 3604Prevents secretion from ER Potential

Amino acid modifications

Glycosylation2711N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P55302 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 72ED46FA0AC05D51

FASTA36042,215
        10         20         30         40         50         60 
MAPRRERVST LPRLQLLVLL LLPLMLVPQP IAGHGGKYSR EKNEPEMAAK RESGEEFRME 

        70         80         90        100        110        120 
KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK VEGLDKDGEK EAKLIHNLNV 

       130        140        150        160        170        180 
ILARYGLDGR KDAQMVHSNA LNEDTQDELG DPRLEKLWHK AKTSGKFSSE ELDKLWREFL 

       190        200        210        220        230        240 
HYKEKIQEYN VLLDTLSRAE EGYENLLSPS DMAHIKSDTL ISKHSELKDR LRSINQGLDR 

       250        260        270        280        290        300 
LRKVSHQGYG STTEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ 

       310        320        330        340        350        360 
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS RVSRARHNEL 

« Hide

References

[1]"Mouse heparin binding protein-44 (HBP-44) associates with brushin, a high-molecular-weight glycoprotein antigen common to the kidney and teratocarcinomas."
Nakamoto M., Ozawa M., Jacinto S.D., Furukawa T., Natori Y., Shirahama H., Yonezawa S., Nakayama T., Muramatsu T.
J. Biochem. 114:344-349(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"A heparin binding protein whose expression increases during differentiation of embryonal carcinoma cells to parietal endoderm cells: cDNA cloning and sequence analysis."
Furukawa T., Ozawa M., Huang R.P., Muramatsu T.
J. Biochem. 108:297-302(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-360, TISSUE SPECIFICITY.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 310-322, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S67967 mRNA. Translation: AAC60668.1.
D00622 mRNA. Translation: BAA00500.1.
CCDSCCDS19225.1.
PIRJX0281.
RefSeqNP_038615.2. NM_013587.2.
UniGeneMm.277661.

3D structure databases

ProteinModelPortalP55302.
SMRP55302. Positions 38-360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201205. 1 interaction.
IntActP55302. 5 interactions.
MINTMINT-4087673.
STRING10090.ENSMUSP00000030986.

PTM databases

PhosphoSiteP55302.

2D gel databases

REPRODUCTION-2DPAGEIPI00469307.
P55302.

Proteomic databases

MaxQBP55302.
PaxDbP55302.
PRIDEP55302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030986; ENSMUSP00000030986; ENSMUSG00000029103.
GeneID16976.
KEGGmmu:16976.
UCSCuc008xdo.1. mouse.

Organism-specific databases

CTD4043.
MGIMGI:96829. Lrpap1.

Phylogenomic databases

eggNOGNOG323629.
HOVERGENHBG000197.
InParanoidP55302.
OMAFRMAKLN.
OrthoDBEOG7P5T15.
PhylomeDBP55302.
TreeFamTF320678.

Gene expression databases

ArrayExpressP55302.
BgeeP55302.
GenevestigatorP55302.

Family and domain databases

Gene3D1.20.81.10. 1 hit.
InterProIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMSSF47045. SSF47045. 3 hits.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291044.
PROP55302.
SOURCESearch...

Entry information

Entry nameAMRP_MOUSE
AccessionPrimary (citable) accession number: P55302
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot