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Protein

Alpha-2-macroglobulin receptor-associated protein

Gene

Lrpap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name:
Alpha-2-MRAP
Alternative name(s):
Heparin-binding protein 44
Short name:
HBP-44
Low density lipoprotein receptor-related protein-associated protein 1
Short name:
RAP
Gene namesi
Name:Lrpap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96829. Lrpap1.

Subcellular locationi

  • Rough endoplasmic reticulum lumen By similarity
  • Endoplasmic reticulum-Golgi intermediate compartment lumen By similarity
  • Golgi apparatuscis-Golgi network By similarity
  • Golgi apparatus lumen By similarity
  • Endosome lumen By similarity
  • Cell surface By similarity

  • Note: May be associated with receptors at the cell surface.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000002072529 – 360Alpha-2-macroglobulin receptor-associated proteinAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Modified residuei138PhosphoserineCombined sources1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP55302.
PaxDbiP55302.
PeptideAtlasiP55302.
PRIDEiP55302.

2D gel databases

REPRODUCTION-2DPAGEIPI00469307.
P55302.

PTM databases

iPTMnetiP55302.
PhosphoSitePlusiP55302.

Expressioni

Tissue specificityi

Highly expressed in PYS-2 parietal endoderm cells and in the kidney. The RNA level increased about 10-fold during differentiation of F9 embryonal carcinoma cells to parietal endoderm cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000029103.
ExpressionAtlasiP55302. baseline and differential.
GenevisibleiP55302. MM.

Interactioni

Subunit structurei

Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor. Interacts with LRP2/glycoprotein 330. Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201205. 2 interactors.
IntActiP55302. 5 interactors.
MINTiMINT-4087673.
STRINGi10090.ENSMUSP00000030986.

Structurei

3D structure databases

ProteinModelPortaliP55302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 356LDL receptor bindingSequence analysisAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili184 – 302Sequence analysisAdd BLAST119

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 360Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the alpha-2-MRAP family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG3956. Eukaryota.
ENOG410YJFP. LUCA.
GeneTreeiENSGT00390000004855.
HOVERGENiHBG000197.
InParanoidiP55302.
OMAiNQVWEKA.
OrthoDBiEOG091G0EO4.
PhylomeDBiP55302.
TreeFamiTF320678.

Family and domain databases

Gene3Di1.20.81.10. 1 hit.
InterProiIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMiSSF47045. SSF47045. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55302-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRRERVST LPRLQLLVLL LLPLMLVPQP IAGHGGKYSR EKNEPEMAAK
60 70 80 90 100
RESGEEFRME KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK
110 120 130 140 150
VEGLDKDGEK EAKLIHNLNV ILARYGLDGR KDAQMVHSNA LNEDTQDELG
160 170 180 190 200
DPRLEKLWHK AKTSGKFSSE ELDKLWREFL HYKEKIQEYN VLLDTLSRAE
210 220 230 240 250
EGYENLLSPS DMAHIKSDTL ISKHSELKDR LRSINQGLDR LRKVSHQGYG
260 270 280 290 300
STTEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ
310 320 330 340 350
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS
360
RVSRARHNEL
Length:360
Mass (Da):42,215
Last modified:October 1, 1996 - v1
Checksum:i72ED46FA0AC05D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67967 mRNA. Translation: AAC60668.1.
D00622 mRNA. Translation: BAA00500.1.
CCDSiCCDS19225.1.
PIRiJX0281.
RefSeqiNP_038615.2. NM_013587.2.
UniGeneiMm.277661.

Genome annotation databases

EnsembliENSMUST00000030986; ENSMUSP00000030986; ENSMUSG00000029103.
GeneIDi16976.
KEGGimmu:16976.
UCSCiuc008xdo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67967 mRNA. Translation: AAC60668.1.
D00622 mRNA. Translation: BAA00500.1.
CCDSiCCDS19225.1.
PIRiJX0281.
RefSeqiNP_038615.2. NM_013587.2.
UniGeneiMm.277661.

3D structure databases

ProteinModelPortaliP55302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201205. 2 interactors.
IntActiP55302. 5 interactors.
MINTiMINT-4087673.
STRINGi10090.ENSMUSP00000030986.

PTM databases

iPTMnetiP55302.
PhosphoSitePlusiP55302.

2D gel databases

REPRODUCTION-2DPAGEIPI00469307.
P55302.

Proteomic databases

EPDiP55302.
PaxDbiP55302.
PeptideAtlasiP55302.
PRIDEiP55302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030986; ENSMUSP00000030986; ENSMUSG00000029103.
GeneIDi16976.
KEGGimmu:16976.
UCSCiuc008xdo.1. mouse.

Organism-specific databases

CTDi4043.
MGIiMGI:96829. Lrpap1.

Phylogenomic databases

eggNOGiKOG3956. Eukaryota.
ENOG410YJFP. LUCA.
GeneTreeiENSGT00390000004855.
HOVERGENiHBG000197.
InParanoidiP55302.
OMAiNQVWEKA.
OrthoDBiEOG091G0EO4.
PhylomeDBiP55302.
TreeFamiTF320678.

Miscellaneous databases

PROiP55302.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029103.
ExpressionAtlasiP55302. baseline and differential.
GenevisibleiP55302. MM.

Family and domain databases

Gene3Di1.20.81.10. 1 hit.
InterProiIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMiSSF47045. SSF47045. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMRP_MOUSE
AccessioniPrimary (citable) accession number: P55302
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.