Alpha-2-macroglobulin receptor-associated protein precursor

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P55302 (AMRP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name=Alpha-2-MRAP

Alternative name(s):
Heparin-binding protein 44
Short name=HBP-44
Low density lipoprotein receptor-related protein-associated protein 1
Short name=RAP

Gene names

Name:

Lrpap1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
Subunit structure	Present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. Binds LRP1B; binding is followed by internalization and degradation By similarity.
Subcellular location	Endoplasmic reticulum By similarity. Cytoplasm By similarity. Cell surface By similarity. Note: Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum By similarity.
Tissue specificity	Highly expressed in PYS-2 parietal endoderm cells and in the kidney. The RNA level increased about 10-fold during differentiation of F9 embryonal carcinoma cells to parietal endoderm cells. Ref.2
Sequence similarities	Belongs to the alpha-2-MRAP family.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum
Domain	Coiled coil Signal
Ligand	Heparin-binding
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	extracellular negative regulation of signal transduction Inferred from electronic annotation. Source: Compara negative regulation of beta-amyloid clearance Inferred from electronic annotation. Source: Compara negative regulation of protein binding Inferred from electronic annotation. Source: Compara negative regulation of very-low-density lipoprotein particle clearance Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: Compara plasma membrane Inferred from sequence orthology PubMed 16263759. Source: MGI rough endoplasmic reticulum lumen Inferred from electronic annotation. Source: Compara vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW low-density lipoprotein particle receptor binding Inferred from sequence orthology PubMed 16263759. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Potential

Chain

29 – 360

332

Alpha-2-macroglobulin receptor-associated protein

PRO_0000020725

Regions

Region

240 – 356

117

LDL receptor binding Potential

Coiled coil

184 – 302

119

Potential

Motif

357 – 360

Prevents secretion from ER Potential

Amino acid modifications

Glycosylation

271

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P55302 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 72ED46FA0AC05D51
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FASTA

360

42,215

        10         20         30         40         50         60 
MAPRRERVST LPRLQLLVLL LLPLMLVPQP IAGHGGKYSR EKNEPEMAAK RESGEEFRME 

        70         80         90        100        110        120 
KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK VEGLDKDGEK EAKLIHNLNV 

       130        140        150        160        170        180 
ILARYGLDGR KDAQMVHSNA LNEDTQDELG DPRLEKLWHK AKTSGKFSSE ELDKLWREFL 

       190        200        210        220        230        240 
HYKEKIQEYN VLLDTLSRAE EGYENLLSPS DMAHIKSDTL ISKHSELKDR LRSINQGLDR 

       250        260        270        280        290        300 
LRKVSHQGYG STTEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ 

       310        320        330        340        350        360 
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS RVSRARHNEL

« Hide

References

[1]	"Mouse heparin binding protein-44 (HBP-44) associates with brushin, a high-molecular-weight glycoprotein antigen common to the kidney and teratocarcinomas." Nakamoto M., Ozawa M., Jacinto S.D., Furukawa T., Natori Y., Shirahama H., Yonezawa S., Nakayama T., Muramatsu T. J. Biochem. 114:344-349(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"A heparin binding protein whose expression increases during differentiation of embryonal carcinoma cells to parietal endoderm cells: cDNA cloning and sequence analysis." Furukawa T., Ozawa M., Huang R.P., Muramatsu T. J. Biochem. 108:297-302(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-360, TISSUE SPECIFICITY.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 310-322, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S67967 mRNA. Translation: AAC60668.1. D00622 mRNA. Translation: BAA00500.1.
IPI	IPI00469307.
PIR	JX0281.
RefSeq	NP_038615.2. NM_013587.2.
UniGene	Mm.277661.
3D structure databases
ProteinModelPortal	P55302.
SMR	P55302. Positions 38-360.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55302. 1 interaction.
STRING	10090.ENSMUSP00000030986.
PTM databases
PhosphoSite	P55302.
2D gel databases
REPRODUCTION-2DPAGE	IPI00469307. P55302.
Proteomic databases
PaxDb	P55302.
PRIDE	P55302.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030986; ENSMUSP00000030986; ENSMUSG00000029103.
GeneID	16976.
KEGG	mmu:16976.
Organism-specific databases
CTD	4043.
MGI	MGI:96829. Lrpap1.
Phylogenomic databases
eggNOG	NOG323629.
HOVERGEN	HBG000197.
InParanoid	P55302.
OMA	FRMAKLN.
OrthoDB	EOG479F7K.
Gene expression databases
ArrayExpress	P55302.
Bgee	P55302.
Genevestigator	P55302.
GermOnline	ENSMUSG00000029103. Mus musculus.
Family and domain databases
Gene3D	1.20.81.10. 1 hit.
InterPro	IPR010483. Alpha_2_MRAP_C. IPR009066. MG_RAP_rcpt_1. [Graphical view]
Pfam	PF06401. Alpha-2-MRAP_C. 1 hit. PF06400. Alpha-2-MRAP_N. 1 hit. [Graphical view]
SUPFAM	SSF47045. MG_RAP_rcpt_1. 3 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291044.
SOURCE	Search...

Entry information

Entry name

AMRP_MOUSE

Accession

Primary (citable) accession number: P55302

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents