Reviewed, UniProtKB/Swiss-Prot P55300 (MTD_METTM)

Names and origin

Protein names

Recommended name:
    F420-dependent methylenetetrahydromethanopterin dehydrogenase
      Short name=MTD
    EC=1.5.99.9
Alternative name(s):
    Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:

mtd

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420.
Enzyme regulation	Oxygen-stable, strictly dependent on coenzyme F420, thermostable, thermophilic and exhibits maximum activity at pH 4 and 55-65 degrees Celsius.
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (cofactor F420 route): step 1/1.
Subunit structure	Found to be tightly associated with methyl-coenzyme M methylreductase.
Sequence similarities	Belongs to the MTD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 276	275	F420-dependent methylenetetrahydromethanopterin dehydrogenase		PRO_0000075042

Sequences

	Sequence		Length	Mass (Da)
	P55300-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E32142964BF3BAC3	FASTA	276	29,614
	10 20 30 40 50 60 MVVKIGIIKC GNIGTSPVLD LLLDERADRP NIDVCVVGSG AKMNPDEIER AVPTMLEMER 70 80 90 100 110 120 DFVIFISPNP GAPGPAKARE LLSAADVPAM IIGDAPGLRV KDEIEEQGLG YIIVKADPMI 130 140 150 160 170 180 GARREFLDPT EMASFNSDVI KVLAFTGAYR VVQNTIDAMI ADVEAGKAPE LPQVVIDTDK 190 200 210 220 230 240 AVEAAGYTNP YAKAKAMAAY EIATKVADID VRGCFMVQDP DQYIPIVASA HEMLSAAAKL 250 260 270 AIEAREIEKA NDTVLRTPHG KEGKTLSKKD LLAKPE

References

[1]

"Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coli." Mukhopadhyay B., Purwantini E., Pihl T.D., Reeve J.N., Daniels L. J. Biol. Chem. 270:2827-2832(1995) [PubMed: 7852356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L37108 Genomic DNA. Translation: AAC41463.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_00058. [Tree]
InterPro	IPR002844. Methylene_DHase. [Graphical view]
Pfam	PF01993. MTD. 1 hit. [Graphical view]
PIRSF	PIRSF005627. MTD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTD_METTM

Accession

Primary (citable) accession number: P55300

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 47 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)