Reviewed, UniProtKB/Swiss-Prot P55298 (MANC_PIRSP)

Names and origin

Protein names

Recommended name:
    Mannan endo-1,4-beta-mannosidase C
    EC=3.2.1.78
Alternative name(s):
    Beta-mannanase C
    1,4-beta-D-mannan mannanohydrolase C

Gene names

Name:

MANC

Organism

Piromyces sp.

Taxonomic identifier

45796 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Neocallimastigomycota › Neocallimastigomycetes › Neocallimastigales › Neocallimastigaceae › Piromyces

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Sequence similarities	Belongs to the glycosyl hydrolase 26 family. Contains 1 CBM6 (carbohydrate binding type-6) domain.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	substituted mannan metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 569	551	Mannan endo-1,4-beta-mannosidase C		PRO_0000012176
Regions
	Domain	21 – 139	119	CBM6
	Repeat	488 – 525	38	1
	Repeat	531 – 569	39	2
	Region	490 – 569	80	2 X 39 AA approximate repeats
	Compositional bias	473 – 477	5	Poly-Asn
	Compositional bias	480 – 486	7	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	P55298-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 19277764E18328B5	FASTA	569	64,115
	10 20 30 40 50 60 MLTLTLLLYL SFITTVFSKK VIYEAENGKL DGVSKYNELQ GYSGTGYVGR FESAGNSVTV 70 80 90 100 110 120 TVEVSQTGMY DMSIIYCGNM GQKINSLKIN GKNSGDITFP ENSSFEELNI GAVYLNSGEN 130 140 150 160 170 180 TVSLVASWGW IWIDALVVND TPNVAKDVSP HINPTLVNPK AIPAAKKLYD FLRSNYGKRI 190 200 210 220 230 240 LSGQVGGAGQ AGDEGQEIQR IQKATGKLPA VWNMDFIFES NDCTWRPENP DITEMAINWW 250 260 270 280 290 300 KKYQGKGIMA AQWHWNIAGK TGDFAFYKKD TTFSIDNAVT EGTWEYEKII KDIDRVAGHI 310 320 330 340 350 360 KKLQAVNMPL IWRPLHENDG DWFWWGNNPK SCAKLWKILY ERMVNYHGLN NLIWLWNGKN 370 380 390 400 410 420 DANTPVDYID IIGVDIYAND HGPQTTAYNT HFDFYGGKKM VVLSENGRIP DIQQCVDQNA 430 440 450 460 470 480 WWGYFQTWNS EFILQDSYHT DAQLKEYFTH KTVMNMDELP SFNVNSYEYQ SGNNNNNSSN 490 500 510 520 530 540 NNNNNNSSEC FSIPLGYPCC KGNTVVYTDN DGDWGVENNE WCGIGNSSSA VVCWSEALGY 550 560 PCCVSSSDVY YTDNDGEWGV ENGDWCGII

References

[1]	"Evidence that the Piromyces gene family encoding endo-1,4-mannanases arose through gene duplication." Millward-Sadler S.J., Hall J., Black G.W., Hazlewood G.P., Gilbert H.J. FEMS Microbiol. Lett. 141:183-188(1996) [PubMed: 8768520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97520 mRNA. Translation: CAA66134.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM35. Carbohydrate-Binding Module Family 35. GH26. Glycoside Hydrolase Family 26.
Family and domain databases
InterPro	IPR005084. Carb-bd_dom_fam6. IPR002883. Dockerin_cellulose-bd_dom. IPR009034. Dockering_cellulose_docking. IPR000805. Glyco_hydro_26. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.90.1220.10. Dockerin_CBD_5. 2 hits. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02013. CBM_10. 2 hits. PF03422. CBM_6. 1 hit. PF02156. Glyco_hydro_26. 1 hit. [Graphical view]
PRINTS	PR00739. GLHYDRLASE26.
PROSITE	PS51175. CBM6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANC_PIRSP

Accession

Primary (citable) accession number: P55298

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 22, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)