P55297 (MANB_PIRSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase B
EC=3.2.1.78

Alternative name(s):
1,4-beta-D-mannan mannanohydrolase B
Beta-mannanase B

Gene names

Name:

MANB

Organism

Piromyces sp.

Taxonomic identifier

45796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Neocallimastigomycota › Neocallimastigomycetes › Neocallimastigales › Neocallimastigaceae › Piromyces

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Sequence similarities	Belongs to the glycosyl hydrolase 26 family. Contains 1 CBM6 (carbohydrate binding type-6) domain.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	substituted mannan metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 571	552	Mannan endo-1,4-beta-mannosidase B		PRO_0000012175
Regions
	Domain	22 – 141	120	CBM6
	Repeat	490 – 527	38	1
	Repeat	533 – 571	39	2
	Region	490 – 571	82	2 X 39 AA approximate repeats
	Compositional bias	475 – 479	5	Poly-Asn
	Compositional bias	482 – 488	7	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	P55297 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B13E44581FAA9DAA	FASTA	571	64,397
	10 20 30 40 50 60 MNSLSLLLFC IFFVFSTFAK KVYYEAENGK LDGVTVYKSD LTGFSGTGYV GRFENPGNSV 70 80 90 100 110 120 TVTVEVPQTG MYDLTIVYCA NMGQKINSLT VNGQSAGDIT FTENTKFEDL NVGAIYLNKG 130 140 150 160 170 180 KNTIGLVSSW GWMWVDAFVI NDAPNAAKDV TSKLNPTLIN PKAIPAAKKL YDFLKTNYGK 190 200 210 220 230 240 RILSGQVGAA GQAGDEGQEI QRIQKATGKL PAVWNMDFIF ESNDCTWRPQ NPDITEMAIN 250 260 270 280 290 300 WWKKYQGKGI MSAQWHWNIA GKTGDFAFYK KDTTFSIDNA VTEGTWEYEK IIKDIDRVAG 310 320 330 340 350 360 HIKKLQAVNM PLIWRPLHEN DGDWFWWGNN PKSCAKLWKI LYERMVNYHG LNNLIWLWNG 370 380 390 400 410 420 KNDANTPVDY IDIIGVDIYA NDHGPQTTAY NTHFDFYGGK KMVVLSENGR IPDIQQCVDQ 430 440 450 460 470 480 NAWWGYFQTW NSEFILQDSY HTDAQLKEYF THKTVMNMDE LPSFNVNSYE YQSGNNNNNS 490 500 510 520 530 540 SNNNNNNNSS ECFSIPLGYP CCKGNTVVYT DNDGDWGVEN NEWCGIGNSS SAVVCWSEAL 550 560 570 GYPCCVSSSD VYYTDNDGEW GVENGDWCGI I

References

[1]	"Evidence that the Piromyces gene family encoding endo-1,4-mannanases arose through gene duplication." Millward-Sadler S.J., Hall J., Black G.W., Hazlewood G.P., Gilbert H.J. FEMS Microbiol. Lett. 141:183-188(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97408 mRNA. Translation: CAA66061.1.
3D structure databases
ProteinModelPortal	P55297.
SMR	P55297. Positions 534-571.
ModBase	Search...
Protein family/group databases
CAZy	CBM35. Carbohydrate-Binding Module Family 35. GH26. Glycoside Hydrolase Family 26.
mycoCLAP	MAN26B_PIRSP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.260. 1 hit. 3.20.20.80. 1 hit. 3.90.1220.10. 2 hits.
InterPro	IPR002883. CBM10/Dockerin_dom. IPR005084. CMB_fam6. IPR009034. Dockerin_dom. IPR022790. EndoGluc_H/Glyco_hydro_26. IPR008979. Galactose-bd-like. IPR000805. Glyco_hydro_26. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02013. CBM_10. 2 hits. PF02156. Glyco_hydro_26. 1 hit. [Graphical view]
PRINTS	PR00739. GLHYDRLASE26.
SUPFAM	SSF64571. Dockering_CDD. 2 hits. SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51175. CBM6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANB_PIRSP

Accession

Primary (citable) accession number: P55297

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program