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P55296

- MANA_PIRSP

UniProt

P55296 - MANA_PIRSP

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Protein
Mannan endo-1,4-beta-mannosidase A
Gene
MANA
Organism
Piromyces sp.
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes 1,4-beta linked polysaccharide backbones of mannans, one of the major hemicellulose components in hardwoods and softwoods. Shows very high activity against mannohexaose but not against mannopentaose and smaller mannooligosaccharides. The major products released from mannooligosaccharide hydrolysis are mannose and mannobiose. The reiterated 40 AA domain is involved in binding the cellulase-hemicellulase complex.

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. cellulase activity Source: InterPro
  3. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. substituted mannan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiCBM35. Carbohydrate-Binding Module Family 35.
GH26. Glycoside Hydrolase Family 26.
mycoCLAPiMAN26A_PIRSP.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase A (EC:3.2.1.78)
Alternative name(s):
1,4-beta-D-mannan mannanohydrolase A
Beta-mannanase A
Gene namesi
Name:MANA
OrganismiPiromyces sp.
Taxonomic identifieri45796 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaePiromyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 606587Mannan endo-1,4-beta-mannosidase A
PRO_0000012174Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP55296.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 140119CBM6
Add
BLAST
Domaini491 – 52636CBM10 1
Add
BLAST
Domaini530 – 56536CBM10 2
Add
BLAST
Domaini569 – 60436CBM10 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 471331Catalytic
Add
BLAST
Regioni472 – 48918Linker
Add
BLAST

Domaini

Consists of a catalytic N-terminal domain linked to a reiterated non-catalytic C-terminal domain.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
3.90.1220.10. 3 hits.
InterProiIPR002883. CBM10/Dockerin_dom.
IPR005084. CMB_fam6.
IPR009034. Dockerin_dom_fun.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02013. CBM_10. 3 hits.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view]
PRINTSiPR00739. GLHYDRLASE26.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 3 hits.
PROSITEiPS51175. CBM6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55296-1 [UniParc]FASTAAdd to Basket

« Hide

MKSLNVILTL LSLIISVLSK KVYYEAEDGK LNGITVFKEL SGFSGKGYVG    50
RFENPGNSVT VTVDAPATGM YDLSIIYCAN MGQKINSLTV NDQSVGDITF 100
TENTKFETKD VGAVYLNKGK NTIGLVSSWG WMWVDAFVIN DAPNAAKDVS 150
SKLNPTLVNP KAIPAAKKLY DFLKTNYGKR ILSGQVGAAG QAGDEGQEIQ 200
RIQKATGKLP AVWNMDFIFE SNDCTWRPQN PDITEMAINW WKKYEGKGIM 250
AAQWHWNIAG KTGDFAFYSK DTTFNLENAV TEGTWEYEKI IKDIDRVSGH 300
IKKLQAVNMP LIWRPLHENN GDWFWWGNNP KACAKLWKIL YERMVNYHGL 350
NNLIWLWNGN NDANTPVDYI DIIGVDIYAN DHGPQTTAYN THFDFYGGKK 400
MVVLSENGRI PDIQQCVDQD VWWGYFQTWN SEFILQDSYH TDAQLKEYFN 450
HKTVMNMDEL PSFNVDSYNG DSGSSHNGNS ESNSNTGNSD ECWSINLGYP 500
CCIGDYVVTT DENGDWGVEN NEWCGIVHKS CWSEPLGYPC CVGNTVISAD 550
ESGDWGVENN EWCGIVHKSC WAEFLGYPCC VGNTVISTDE FGDWGVENDD 600
WCGILN 606
Length:606
Mass (Da):68,055
Last modified:October 1, 1996 - v1
Checksum:i79AAFEEFA2725D86
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91857 mRNA. Translation: CAA62968.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91857 mRNA. Translation: CAA62968.1 .

3D structure databases

ProteinModelPortali P55296.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM35. Carbohydrate-Binding Module Family 35.
GH26. Glycoside Hydrolase Family 26.
mycoCLAPi MAN26A_PIRSP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
3.90.1220.10. 3 hits.
InterProi IPR002883. CBM10/Dockerin_dom.
IPR005084. CMB_fam6.
IPR009034. Dockerin_dom_fun.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02013. CBM_10. 3 hits.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view ]
PRINTSi PR00739. GLHYDRLASE26.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 3 hits.
PROSITEi PS51175. CBM6. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain."
    Fanutti C., Ponyi T., Black G.W., Hazlewood G.P., Gilbert H.J.
    J. Biol. Chem. 270:29314-29322(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiMANA_PIRSP
AccessioniPrimary (citable) accession number: P55296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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