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P55296

- MANA_PIRSP

UniProt

P55296 - MANA_PIRSP

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Protein

Mannan endo-1,4-beta-mannosidase A

Gene

MANA

Organism
Piromyces sp.
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolyzes 1,4-beta linked polysaccharide backbones of mannans, one of the major hemicellulose components in hardwoods and softwoods. Shows very high activity against mannohexaose but not against mannopentaose and smaller mannooligosaccharides. The major products released from mannooligosaccharide hydrolysis are mannose and mannobiose. The reiterated 40 AA domain is involved in binding the cellulase-hemicellulase complex.

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. cellulase activity Source: InterPro
  3. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. substituted mannan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiCBM35. Carbohydrate-Binding Module Family 35.
GH26. Glycoside Hydrolase Family 26.
mycoCLAPiMAN26A_PIRSP.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase A (EC:3.2.1.78)
Alternative name(s):
1,4-beta-D-mannan mannanohydrolase A
Beta-mannanase A
Gene namesi
Name:MANA
OrganismiPiromyces sp.
Taxonomic identifieri45796 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaePiromyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 606587Mannan endo-1,4-beta-mannosidase APRO_0000012174Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP55296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 140119CBM6PROSITE-ProRule annotationAdd
BLAST
Domaini491 – 52636CBM10 1Add
BLAST
Domaini530 – 56536CBM10 2Add
BLAST
Domaini569 – 60436CBM10 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 471331CatalyticAdd
BLAST
Regioni472 – 48918LinkerAdd
BLAST

Domaini

Consists of a catalytic N-terminal domain linked to a reiterated non-catalytic C-terminal domain.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 26 family.Curated
Contains 1 CBM6 (carbohydrate binding type-6) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
3.90.1220.10. 3 hits.
InterProiIPR002883. CBM10/Dockerin_dom.
IPR005084. CMB_fam6.
IPR009034. Dockerin_dom_fun.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02013. CBM_10. 3 hits.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view]
PRINTSiPR00739. GLHYDRLASE26.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 3 hits.
PROSITEiPS51175. CBM6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55296-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSLNVILTL LSLIISVLSK KVYYEAEDGK LNGITVFKEL SGFSGKGYVG
60 70 80 90 100
RFENPGNSVT VTVDAPATGM YDLSIIYCAN MGQKINSLTV NDQSVGDITF
110 120 130 140 150
TENTKFETKD VGAVYLNKGK NTIGLVSSWG WMWVDAFVIN DAPNAAKDVS
160 170 180 190 200
SKLNPTLVNP KAIPAAKKLY DFLKTNYGKR ILSGQVGAAG QAGDEGQEIQ
210 220 230 240 250
RIQKATGKLP AVWNMDFIFE SNDCTWRPQN PDITEMAINW WKKYEGKGIM
260 270 280 290 300
AAQWHWNIAG KTGDFAFYSK DTTFNLENAV TEGTWEYEKI IKDIDRVSGH
310 320 330 340 350
IKKLQAVNMP LIWRPLHENN GDWFWWGNNP KACAKLWKIL YERMVNYHGL
360 370 380 390 400
NNLIWLWNGN NDANTPVDYI DIIGVDIYAN DHGPQTTAYN THFDFYGGKK
410 420 430 440 450
MVVLSENGRI PDIQQCVDQD VWWGYFQTWN SEFILQDSYH TDAQLKEYFN
460 470 480 490 500
HKTVMNMDEL PSFNVDSYNG DSGSSHNGNS ESNSNTGNSD ECWSINLGYP
510 520 530 540 550
CCIGDYVVTT DENGDWGVEN NEWCGIVHKS CWSEPLGYPC CVGNTVISAD
560 570 580 590 600
ESGDWGVENN EWCGIVHKSC WAEFLGYPCC VGNTVISTDE FGDWGVENDD

WCGILN
Length:606
Mass (Da):68,055
Last modified:October 1, 1996 - v1
Checksum:i79AAFEEFA2725D86
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91857 mRNA. Translation: CAA62968.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91857 mRNA. Translation: CAA62968.1 .

3D structure databases

ProteinModelPortali P55296.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM35. Carbohydrate-Binding Module Family 35.
GH26. Glycoside Hydrolase Family 26.
mycoCLAPi MAN26A_PIRSP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
3.90.1220.10. 3 hits.
InterProi IPR002883. CBM10/Dockerin_dom.
IPR005084. CMB_fam6.
IPR009034. Dockerin_dom_fun.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02013. CBM_10. 3 hits.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view ]
PRINTSi PR00739. GLHYDRLASE26.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 3 hits.
PROSITEi PS51175. CBM6. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain."
    Fanutti C., Ponyi T., Black G.W., Hazlewood G.P., Gilbert H.J.
    J. Biol. Chem. 270:29314-29322(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiMANA_PIRSP
AccessioniPrimary (citable) accession number: P55296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3