Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cadherin-5

Gene

Cdh5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Calcium 1By similarity
Metal bindingi56 – 561Calcium 2By similarity
Metal bindingi57 – 571Calcium 1By similarity
Metal bindingi107 – 1071Calcium 1By similarity
Metal bindingi109 – 1091Calcium 1By similarity
Metal bindingi109 – 1091Calcium 2By similarity
Metal bindingi141 – 1411Calcium 2By similarity
Metal bindingi142 – 1421Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi143 – 1431Calcium 3By similarity
Metal bindingi144 – 1441Calcium 1By similarity
Metal bindingi144 – 1441Calcium 2By similarity
Metal bindingi145 – 1451Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi175 – 1751Calcium 3By similarity
Metal bindingi177 – 1771Calcium 2By similarity
Metal bindingi177 – 1771Calcium 3By similarity
Metal bindingi184 – 1841Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi229 – 2291Calcium 3By similarity

GO - Molecular functioni

GO - Biological processi

  • blood vessel maturation Source: MGI
  • cell-cell junction assembly Source: MGI
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
  • negative regulation of cell proliferation Source: MGI
  • positive regulation of establishment of endothelial barrier Source: MGI
  • regulation of establishment of cell polarity Source: UniProtKB
  • single organismal cell-cell adhesion Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-418990. Adherens junctions interactions.
R-MMU-5218920. VEGFR2 mediated vascular permeability.

Names & Taxonomyi

Protein namesi
Recommended name:
Cadherin-5
Alternative name(s):
Vascular endothelial cadherin
Short name:
VE-cadherin
CD_antigen: CD144
Gene namesi
Name:Cdh5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105057. Cdh5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini46 – 599554ExtracellularSequence analysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence analysisAdd
BLAST
Topological domaini621 – 784164CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • adherens junction Source: MGI
  • bicellular tight junction Source: MGI
  • cell-cell junction Source: MGI
  • cell junction Source: UniProtKB
  • cell periphery Source: MGI
  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi774 – 7741Y → A: No effect on PARD3 binding. 1 Publication
Mutagenesisi777 – 7771D → A: Impairs PARD3 binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 4521Sequence analysisPRO_0000003757Add
BLAST
Chaini46 – 784739Cadherin-5PRO_0000003758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence analysis
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence analysis
Glycosylationi535 – 5351N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.1 Publication
O-glycosylated.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP55284.
PaxDbiP55284.
PeptideAtlasiP55284.
PRIDEiP55284.

PTM databases

iPTMnetiP55284.
PhosphoSiteiP55284.

Expressioni

Developmental stagei

Expressed in endothelial cells of allantois/umbilical vessels at 8.5 dpc (at protein level). During hemangioblast differentiation, expressed in hemogenic endothelium cells and down-regulated in nascent blood precursors.2 Publications

Inductioni

Up-regulated by SOX7.1 Publication

Gene expression databases

BgeeiP55284.
CleanExiMM_CDH5.
GenevisibleiP55284. MM.

Interactioni

Subunit structurei

Interacts (via cadherin 5 domain) with PTPRB. Interacts with TRPC4. Interacts with KRIT1 (By similarity). Interacts with PARD3.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tgfbr1Q647292EBI-7087433,EBI-2899393
Tgfbr2Q623124EBI-7087433,EBI-2899332

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • ion channel binding Source: MGI
  • protein phosphatase binding Source: UniProtKB
  • receptor binding Source: MGI

Protein-protein interaction databases

BioGridi198640. 3 interactions.
IntActiP55284. 10 interactions.
MINTiMINT-247286.
STRINGi10090.ENSMUSP00000034339.

Structurei

Secondary structure

1
784
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi773 – 7764Combined sources
Beta strandi781 – 7833Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KOHNMR-B769-784[»]
ProteinModelPortaliP55284.
SMRiP55284. Positions 46-576, 679-775.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55284.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 149104Cadherin 1PROSITE-ProRule annotationAdd
BLAST
Domaini150 – 256107Cadherin 2PROSITE-ProRule annotationAdd
BLAST
Domaini257 – 371115Cadherin 3PROSITE-ProRule annotationAdd
BLAST
Domaini372 – 476105Cadherin 4PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 593117Cadherin 5PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi738 – 75316Ser-richAdd
BLAST

Domaini

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.By similarity

Sequence similaritiesi

Contains 5 cadherin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
GeneTreeiENSGT00760000118954.
HOGENOMiHOG000231252.
HOVERGENiHBG005217.
InParanoidiP55284.
KOiK06533.
OMAiHGKSVPE.
OrthoDBiEOG773XFF.
PhylomeDBiP55284.
TreeFamiTF329887.

Family and domain databases

Gene3Di2.60.40.60. 5 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR027397. Catenin_binding_dom.
IPR030052. CDH5.
[Graphical view]
PANTHERiPTHR24027:SF89. PTHR24027:SF89. 1 hit.
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 5 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRLTELATA LGAFLGLLAV AAMAGPNFPQ IDTPNMLPAH HRQKRDWIWN
60 70 80 90 100
QMHIDEEKNE SLPHYVGKIK SNVNRQNAKY VLQGEFAGKI FGVDANTGNV
110 120 130 140 150
LAYERLDREK VSEYFLTALI VDKNTNKNLE QPSSFTVKVH DINDNWPVFS
160 170 180 190 200
HQVFNASVPE MSAIGTSVIR VTAVDADDPT VAGHATVLYQ IVKGNEYFSI
210 220 230 240 250
DNSGLIFTKI KNLDREKQAE YKIVVETQDA LGLRGESGTA TVMIRLEDIN
260 270 280 290 300
DNFPVFTQST YTFSVPEDIR VGKPLGFLTV VDPDEPQNRM TKYSIMQGEY
310 320 330 340 350
RDTFTIETDP KRNEGIIKPT KSLDYEVIQQ YTFYIEATDP TIRYEYLSST
360 370 380 390 400
SGKNKAMVTI NVLDVDEPPV FQRHFYHFKL PENQKKPLIG TVVAKDPDKA
410 420 430 440 450
QRSIGYSIRK TSDRGQFFRI TKQGNIYNEK ELDRETYAWY NLTVEANELD
460 470 480 490 500
SRGNPVGKES IVQVYIEVLD ENDNPPEFAQ PYEPKVCENA AQGKLVVQIS
510 520 530 540 550
ATDKDVVPVN PKFKFALKNE DSNFTLINNH DNTANITVKY GQFNREHAKF
560 570 580 590 600
HYLPVLISDN GVPSLTGTST LTVGVCKCNE QGEFTFCEEM AAQAGVSIQA
610 620 630 640 650
LVAIFLCILT ITVITLLIIL RRRIRKQAHA HSKSALEIHE QLVTYDEEGG
660 670 680 690 700
GEMDTTSYDV SVLNSVRGGS TKPLRSTMDA RPAVYTQVQK PPRLAPGLHG
710 720 730 740 750
GPREMATMID VKKEEADNDG GGPPYDTLHI YGYEGAESIA ESLSSLSTNS
760 770 780
SDSDIDYDFL NDWGPRFKML AELYGSDPQE ELII
Length:784
Mass (Da):87,903
Last modified:September 9, 2003 - v2
Checksum:i7B75698DE2F7E160
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83930 mRNA. Translation: CAA58782.2.
D63942 mRNA. Translation: BAA22617.1.
BC054790 mRNA. Translation: AAH54790.1.
CCDSiCCDS22572.1.
RefSeqiNP_033998.2. NM_009868.4.
XP_006530693.1. XM_006530630.1.
UniGeneiMm.21767.

Genome annotation databases

EnsembliENSMUST00000034339; ENSMUSP00000034339; ENSMUSG00000031871.
GeneIDi12562.
KEGGimmu:12562.
UCSCiuc009mzx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83930 mRNA. Translation: CAA58782.2.
D63942 mRNA. Translation: BAA22617.1.
BC054790 mRNA. Translation: AAH54790.1.
CCDSiCCDS22572.1.
RefSeqiNP_033998.2. NM_009868.4.
XP_006530693.1. XM_006530630.1.
UniGeneiMm.21767.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KOHNMR-B769-784[»]
ProteinModelPortaliP55284.
SMRiP55284. Positions 46-576, 679-775.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198640. 3 interactions.
IntActiP55284. 10 interactions.
MINTiMINT-247286.
STRINGi10090.ENSMUSP00000034339.

PTM databases

iPTMnetiP55284.
PhosphoSiteiP55284.

Proteomic databases

MaxQBiP55284.
PaxDbiP55284.
PeptideAtlasiP55284.
PRIDEiP55284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034339; ENSMUSP00000034339; ENSMUSG00000031871.
GeneIDi12562.
KEGGimmu:12562.
UCSCiuc009mzx.2. mouse.

Organism-specific databases

CTDi1003.
MGIiMGI:105057. Cdh5.

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
GeneTreeiENSGT00760000118954.
HOGENOMiHOG000231252.
HOVERGENiHBG005217.
InParanoidiP55284.
KOiK06533.
OMAiHGKSVPE.
OrthoDBiEOG773XFF.
PhylomeDBiP55284.
TreeFamiTF329887.

Enzyme and pathway databases

ReactomeiR-MMU-418990. Adherens junctions interactions.
R-MMU-5218920. VEGFR2 mediated vascular permeability.

Miscellaneous databases

EvolutionaryTraceiP55284.
PROiP55284.
SOURCEiSearch...

Gene expression databases

BgeeiP55284.
CleanExiMM_CDH5.
GenevisibleiP55284. MM.

Family and domain databases

Gene3Di2.60.40.60. 5 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR027397. Catenin_binding_dom.
IPR030052. CDH5.
[Graphical view]
PANTHERiPTHR24027:SF89. PTHR24027:SF89. 1 hit.
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 5 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of murine vascular endothelial-cadherin in early stage development of cardiovascular system."
    Breier G., Breviario F., Caveda L., Berthier R., Schnuerch H., Gotsch U., Vestweber D., Risau W., Dejana E.
    Blood 87:630-641(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain capillary.
  2. Breviario F.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 67-70.
  3. "In vivo evidence of the critical role of cadherin-5 in murine vascular integrity."
    Matsuyoshi N., Toda K., Horiguchi Y., Tanaka T., Nakagawa S., Takeichi M., Imamura S.
    Proc. Assoc. Am. Physicians 109:362-371(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: BALB/cJ.
    Tissue: Mammary carcinoma.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "VE-PTP and VE-cadherin ectodomains interact to facilitate regulation of phosphorylation and cell contacts."
    Nawroth R., Poell G., Ranft A., Kloep S., Samulowitz U., Fachinger G., Golding M., Shima D.T., Deutsch U., Vestweber D.
    EMBO J. 21:4885-4895(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTPRB, PHOSPHORYLATION, DEPHOSPHORYLATION BY PTPRB.
  6. "Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1."
    Kanemura H., Satoh T., Bilasy S.E., Ueda S., Hirashima M., Kataoka T.
    Biochem. Biophys. Res. Commun. 387:754-759(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung and Spleen.
  8. "CCM1 regulates vascular-lumen organization by inducing endothelial polarity."
    Lampugnani M.G., Orsenigo F., Rudini N., Maddaluno L., Boulday G., Chapon F., Dejana E.
    J. Cell Sci. 123:1073-1080(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "SOX7 regulates the expression of VE-cadherin in the haemogenic endothelium at the onset of haematopoietic development."
    Costa G., Mazan A., Gandillet A., Pearson S., Lacaud G., Kouskoff V.
    Development 139:1587-1598(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DEVELOPMENTAL STAGE.
  10. "Distal interactions within the par3-VE-cadherin complex."
    Tyler R.C., Peterson F.C., Volkman B.F.
    Biochemistry 49:951-957(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 769-784 IN COMPLEX WITH PARD3, INTERACTION WITH PARD3, MUTAGENESIS OF TYR-774 AND ASP-777.

Entry informationi

Entry nameiCADH5_MOUSE
AccessioniPrimary (citable) accession number: P55284
Secondary accession number(s): O35542
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 9, 2003
Last modified: July 6, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.