Reviewed, UniProtKB/Swiss-Prot P55278 (MANB2_BACSU)

Names and origin

Protein names	Recommended name:     Mannan endo-1,4-beta-mannosidase     EC=3.2.1.78 Alternative name(s):     Beta-mannanase     1,4-beta-D-mannan mannanohydrolase
Organism	Bacillus subtilis
Taxonomic identifier	1423 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 26 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	substituted mannan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 360	336	Mannan endo-1,4-beta-mannosidase		PRO_0000012171

Sequences

	Sequence		Length	Mass (Da)
	P55278-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 070B0EBCC0382383	FASTA	360	40,295
	10 20 30 40 50 60 MLKKLAVCLS IVLLLLGAAS PISAHTVYPV NPNAQQTTKD IMNWLAHLPN RSENRVMSGA 70 80 90 100 110 120 FGGYSDVTFS MTEENRLKNA TGQSPAIYGC DYGRGWLETA DITDTIDYCC NSSLISYWKS 130 140 150 160 170 180 GGLPQVSLHL ANPAFPSGNY KTAISNSQYK NILDPSTVEG KRLEALLSKI ADGLTQLKNQ 190 200 210 220 230 240 GVTVLFRPLH EMNGEWFWWG LTGYNQKDNE RISLYKELYK KIYRYMTETR GLDNLLWVYS 250 260 270 280 290 300 PDANRDFKTD FYPGSSYVDI TGLDAYFTDP YAISGYDEML SLKKPFAFAE TGPSGNIGSF 310 320 330 340 350 360 DYAAFINAIR QKYPQTTYFL TWDEQLSPAA NQGAQSLYQN SWTLNKGEIW NGGSLTPIAE

References

[1]	"Cloning and sequencing of beta-mannanase gene from Bacillus subtilis NM-39." Mendoza N.S., Arai M., Sugimoto K., Ueda M., Kawaguchi T., Joson L.M. Biochim. Biophys. Acta 1243:552-554(1995) [PubMed: 7727534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NM-39.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D37964 Genomic DNA. Translation: BAA07178.1.
PIR	S60268.
3D structure databases
SMR	P55278. Positions 25-360.
ModBase	Search...
Protein family/group databases
CAZy	GH26. Glycoside Hydrolase Family 26.
Enzyme and pathway databases
BRENDA	3.2.1.78. 150.
Family and domain databases
InterPro	IPR000805. Glyco_hydro_26. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR016714. Mannan-1_4-b-mannosidase. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PIRSF	PIRSF018168. Mannan-1_4-beta-mannosidase. 1 hit.
PRINTS	PR00739. GLHYDRLASE26.
ProtoNet	Search...

Entry information

Entry name

MANB2_BACSU

Accession

Primary (citable) accession number: P55278

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)