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P55273 (CDN2D_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 4 inhibitor D
Alternative name(s):
p19-INK4d
Gene names
Name:CDKN2D
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length166 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts strongly with CDK4 and CDK6 and inhibits them. Ref.2 Ref.3

Subunit structure

Interacts with CDK6. Ref.10

Subcellular location

Nucleus. Cytoplasm Ref.10.

Sequence similarities

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Contains 4 ANK repeats.

Ontologies

Keywords
   Biological processCell cycle
   Cellular componentCytoplasm
Nucleus
   DiseaseTumor suppressor
   DomainANK repeat
Repeat
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processDNA synthesis involved in DNA repair

Inferred from mutant phenotype PubMed 15750620. Source: BHF-UCL

G1/S transition of mitotic cell cycle

Inferred from direct assay PubMed 10208428. Source: BHF-UCL

autophagic cell death

Inferred from mutant phenotype PubMed 16322758. Source: BHF-UCL

cell cycle arrest

Inferred from direct assay PubMed 10208428. Source: BHF-UCL

mitotic cell cycle

Traceable author statement. Source: Reactome

negative regulation of cell growth

Inferred from direct assay PubMed 10208428. Source: BHF-UCL

negative regulation of cell proliferation

Inferred from direct assay PubMed 10208428. Source: BHF-UCL

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from mutant phenotype PubMed 15750620. Source: BHF-UCL

negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from mutant phenotype PubMed 15750620. Source: BHF-UCL

negative regulation of phosphorylation

Inferred from direct assay PubMed 10208428. Source: BHF-UCL

negative regulation of protein serine/threonine kinase activity

Inferred from direct assay Ref.3. Source: GOC

regulation of cyclin-dependent protein serine/threonine kinase activity

Inferred from direct assay Ref.3. Source: BHF-UCL

response to UV

Inferred from mutant phenotype PubMed 15750620. Source: BHF-UCL

response to retinoic acid

Inferred from mutant phenotype PubMed 16322758. Source: BHF-UCL

response to vitamin D

Inferred from mutant phenotype PubMed 16322758. Source: BHF-UCL

sensory perception of sound

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 15750620. Source: BHF-UCL

cytosol

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 15750620Ref.3. Source: BHF-UCL

   Molecular_functioncyclin-dependent protein serine/threonine kinase inhibitor activity

Inferred from direct assay Ref.3. Source: BHF-UCL

protein kinase binding

Inferred from physical interaction Ref.3. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDK6Q005342EBI-745859,EBI-295663

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 166166Cyclin-dependent kinase 4 inhibitor D
PRO_0000144188

Regions

Repeat41 – 6929ANK 1
Repeat73 – 10230ANK 2
Repeat106 – 13530ANK 3
Repeat138 – 16629ANK 4

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9

Experimental info

Sequence conflict1591Q → P in AAA85436. Ref.2

Secondary structure

..................... 166
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P55273 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2FACD11CF56340DC

FASTA16617,700
        10         20         30         40         50         60 
MLLEEVRAGD RLSGAAARGD VQEVRRLLHR ELVHPDALNR FGKTALQVMM FGSTAIALEL 

        70         80         90        100        110        120 
LKQGASPNVQ DTSGTSPVHD AARTGFLDTL KVLVEHGADV NVPDGTGALP IHLAVQEGHT 

       130        140        150        160 
AVVSFLAAES DLHRRDARGL TPLELALQRG AQDLVDILQG HMVAPL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, expression pattern, and chromosomal localization of human CDKN2D/INK4d, an inhibitor of cyclin D-dependent kinases."
Okuda T., Hirai H., Valentine V.A., Shurtleff S.A., Kidd V.J., Lahti J.M., Sherr C.J., Downing J.R.
Genomics 29:623-630(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone marrow.
[2]"Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4."
Chan F.K.M., Zhang J., Cheng L., Shapiro D.N., Winoto A.
Mol. Cell. Biol. 15:2682-2688(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Thymus.
[3]"Isolation and characterization of p19INK4d, a p16-related inhibitor specific to CDK6 and CDK4."
Guan K.L., Jenkins C.W., Li Y., O'Keefe C.L., Noh S., Wu X., Zariwala M., Matera A.G., Xiong Y.
Mol. Biol. Cell 7:57-70(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[4]"Mutation testing in melanoma families: INK4A, CDK4 and INK4D."
Newton Bishop J.A., Harland M., Bennett D.C., Bataille V., Goldstein A.M., Tucker M.A., Ponder B.A.J., Cuzick J., Selby P., Bishop D.T.
Br. J. Cancer 80:295-300(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]NIEHS SNPs program
Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Muscle.
[8]Murthy S.K., Demetrick D.J.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[9]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-7, ACETYLATION AT MET-1.
Tissue: Platelet.
[10]"Active cdk6 complexes are predominantly nuclear and represent only a minority of the cdk6 in T cells."
Mahony D., Parry D.A., Lees E.
Oncogene 16:603-611(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CDK6.
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Structural basis for inhibition of the cyclin-dependent kinase Cdk6 by the tumour suppressor p16INK4a."
Russo A.A., Tong L., Lee J.O., Jeffrey P.D., Pavletich N.P.
Nature 395:237-243(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF COMPLEX WITH CDK6.
[13]"Structure of human cyclin-dependent kinase inhibitor p19(INK4d): comparison to known ankyrin-repeat-containing structures and implications for the dysfunction of tumor suppressor p16(INK4a)."
Baumgartner R., Fernandez-Catalan C., Winoto A., Huber R., Engh R.A., Holak T.A.
Structure 6:1279-1290(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U49399 mRNA. Translation: AAB03772.1.
U20498 mRNA. Translation: AAA85436.1.
U40343 mRNA. Translation: AAB18139.1.
AF061327 Genomic DNA. Translation: AAC27450.1.
CR542158 mRNA. Translation: CAG46955.1.
AF518878 Genomic DNA. Translation: AAM54045.1.
BC001822 mRNA. Translation: AAH01822.1.
AF044171 Genomic DNA. Translation: AAD02320.1.
PIRA57378.
RefSeqNP_001791.1. NM_001800.3.
NP_524145.1. NM_079421.2.
UniGeneHs.435051.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BD8X-ray1.80A7-162[»]
1BI8X-ray2.80B/D1-166[»]
ProteinModelPortalP55273.
SMRP55273. Positions 7-162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107466. 7 interactions.
DIPDIP-6109N.
IntActP55273. 5 interactions.
MINTMINT-1475963.
STRING9606.ENSP00000337056.

PTM databases

PhosphoSiteP55273.

Polymorphism databases

DMDM1705730.

Proteomic databases

PaxDbP55273.
PRIDEP55273.

Protocols and materials databases

DNASU1032.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000335766; ENSP00000337056; ENSG00000129355.
ENST00000393599; ENSP00000377224; ENSG00000129355.
GeneID1032.
KEGGhsa:1032.
UCSCuc002mpa.3. human.

Organism-specific databases

CTD1032.
GeneCardsGC19M010677.
HGNCHGNC:1790. CDKN2D.
HPACAB011201.
MIM600927. gene.
neXtProtNX_P55273.
Orphanet618. Familial melanoma.
PharmGKBPA26323.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0666.
HOGENOMHOG000290191.
HOVERGENHBG050870.
InParanoidP55273.
KOK06623.
OMAVMMFGSS.
OrthoDBEOG7TTQ94.
PhylomeDBP55273.
TreeFamTF333311.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_120956. Cellular responses to stress.

Gene expression databases

BgeeP55273.
CleanExHS_CDKN2D.
GenevestigatorP55273.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamPF00023. Ank. 2 hits.
PF12796. Ank_2. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP55273.
GeneWikiCDKN2D.
GenomeRNAi1032.
NextBio4343.
PROP55273.
SOURCESearch...

Entry information

Entry nameCDN2D_HUMAN
AccessionPrimary (citable) accession number: P55273
Secondary accession number(s): Q13102, Q6FGE9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM