P55272 (CDN2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 4 inhibitor B

Alternative name(s):
p14-INK4b
p15-INK4b

Gene names

Name:	Cdkn2b
Synonyms:	Ink4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest By similarity.
Subunit structure	Heterodimer of CDKN2B with CDK4 or CDK6.
Tissue specificity	Expression abundant in lung, less abundant in testis, barely detectable in liver, and not detectable in neonatal kidney, adult kidney, brain, heart, or spleen.
Sequence similarities	Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. Contains 4 ANK repeats.

Ontologies

Keywords
Biological process	Cell cycle
Coding sequence diversity	Alternative initiation
Disease	Tumor suppressor
Domain	ANK repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara cell cycle arrest Inferred from electronic annotation. Source: Compara cellular response to nutrient Inferred from electronic annotation. Source: Compara megakaryocyte differentiation Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from mutant phenotype Ref.1. Source: RGD negative regulation of epithelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of epithelial cell differentiation Inferred from expression pattern PubMed 17158884. Source: RGD positive regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from expression pattern PubMed 15333329. Source: RGD response to organic cyclic compound Inferred from expression pattern PubMed 11513834. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	cyclin-dependent protein serine/threonine kinase inhibitor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P55272-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P55272-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	Cyclin-dependent kinase 4 inhibitor B		PRO_0000004808
Regions
	Repeat	5 – 34	30	ANK 1
	Repeat	38 – 66	29	ANK 2
	Repeat	71 – 100	30	ANK 3
	Repeat	104 – 130	27	ANK 4
Amino acid modifications
	Modified residue	12	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 45	45	Missing in isoform Short.		VSP_018702

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AC45B21FA69FAD92	FASTA	130	13,749
	10 20 30 40 50 60 MLGGGSDAGL ATAAARGQVE TVRQLLEAGA DPNAVNRFGR RPIQVMMMGS AQVAELLLLH 70 80 90 100 110 120 GAEPNCADPA TLTRPVHDAA REGFLDTLMV LHKAGARLDV CDAWGRLPVD LAEEQGHRDI 130 ARYLHAATGD
	Isoform Short [UniParc]. Checksum: 18B7A5B6D45C86EC	FASTA	85	9,174
	10 20 30 40 50 60 MMMGSAQVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLMVLHKAG ARLDVCDAWG 70 80 RLPVDLAEEQ GHRDIARYLH AATGD

References

[1]	"Molecular genetic basis of renal carcinogenesis in the Eker rat model of tuberous sclerosis (Tsc2)." Hino O., Kobayashi E., Hirayama Y., Kobayashi T., Kubo Y., Tsuchiya H., Kikuchi Y., Mitani H. Mol. Carcinog. 14:23-27(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Buckles L.K., Shull J.D. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ACI/SegHsd and COP. Tissue: Lung.
[3]	"Association of rat p15INK4B/p16INK4 deletions with monosomy 5 in kidney epithelial cell lines but not primary renal tumors." Knapek D.F., Serrano M., Beach D., Trono D., Walker C.L. Cancer Res. 55:1607-1612(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-86.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S79760 mRNA. Translation: AAB35360.1. AF474978 mRNA. Translation: AAL76340.1. AF474979 mRNA. Translation: AAL76341.1. S77734 Genomic DNA. No translation available.
IPI	IPI00215337. IPI00760120.
RefSeq	NP_570825.1. NM_130812.2.
UniGene	Rn.105626.
3D structure databases
ProteinModelPortal	P55272.
SMR	P55272. Positions 1-130.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000008898.
Proteomic databases
PaxDb	P55272.
PRIDE	P55272.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000008898; ENSRNOP00000008898; ENSRNOG00000006735.
GeneID	25164.
KEGG	rno:25164.
UCSC	RGD:2324. rat.
Organism-specific databases
CTD	1030.
RGD	2324. Cdkn2b.
Phylogenomic databases
eggNOG	NOG287192.
GeneTree	ENSGT00390000004527.
HOGENOM	HOG000290191.
HOVERGEN	HBG050870.
InParanoid	P55272.
KO	K04685.
OMA	TRGSNHA.
OrthoDB	EOG49KFS3.
Gene expression databases
ArrayExpress	P55272.
Genevestigator	P55272.
GermOnline	ENSRNOG00000006735. Rattus norvegicus.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Pfam	PF12796. Ank_2. 1 hit. [Graphical view]
SMART	SM00248. ANK. 3 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605637.

Entry information

Entry name

CDN2B_RAT

Accession

Primary (citable) accession number: P55272

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program