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Protein

Cyclin-dependent kinase 4 inhibitor B

Gene

Cdkn2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest (By similarity).By similarity

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: Ensembl

GO - Biological processi

  1. aging Source: RGD
  2. cell cycle arrest Source: Ensembl
  3. cellular response to nutrient Source: Ensembl
  4. G2/M transition of mitotic cell cycle Source: Ensembl
  5. liver development Source: RGD
  6. megakaryocyte differentiation Source: Ensembl
  7. mitotic cell cycle checkpoint Source: Ensembl
  8. negative regulation of cell proliferation Source: RGD
  9. negative regulation of epithelial cell proliferation Source: Ensembl
  10. negative regulation of G1/S transition of mitotic cell cycle Source: Ensembl
  11. positive regulation of epithelial cell differentiation Source: RGD
  12. positive regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
  13. regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  14. response to cytokine Source: RGD
  15. response to organic cyclic compound Source: RGD
  16. spleen development Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

ReactomeiREACT_194748. Senescence-Associated Secretory Phenotype (SASP).
REACT_203770. Oxidative Stress Induced Senescence.
REACT_215142. Oncogene Induced Senescence.
REACT_253353. Cyclin D associated events in G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 4 inhibitor B
Alternative name(s):
p14-INK4b
p15-INK4b
Gene namesi
Name:Cdkn2b
Synonyms:Ink4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi2324. Cdkn2b.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: RGD
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 130130Cyclin-dependent kinase 4 inhibitor BPRO_0000004808Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP55272.
PRIDEiP55272.

Expressioni

Tissue specificityi

Expression abundant in lung, less abundant in testis, barely detectable in liver, and not detectable in neonatal kidney, adult kidney, brain, heart, or spleen.

Gene expression databases

GenevestigatoriP55272.

Interactioni

Subunit structurei

Heterodimer of CDKN2B with CDK4 or CDK6.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008898.

Structurei

3D structure databases

ProteinModelPortaliP55272.
SMRiP55272. Positions 1-130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati5 – 3430ANK 1Add
BLAST
Repeati38 – 6629ANK 2Add
BLAST
Repeati71 – 10030ANK 3Add
BLAST
Repeati104 – 13027ANK 4Add
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiNOG287192.
GeneTreeiENSGT00390000004527.
HOGENOMiHOG000290191.
HOVERGENiHBG050870.
InParanoidiP55272.
KOiK04685.
OMAiVMMMGSV.
OrthoDBiEOG7TTQ94.
PhylomeDBiP55272.
TreeFamiTF352389.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Long (identifier: P55272-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGGGSDAGL ATAAARGQVE TVRQLLEAGA DPNAVNRFGR RPIQVMMMGS
60 70 80 90 100
AQVAELLLLH GAEPNCADPA TLTRPVHDAA REGFLDTLMV LHKAGARLDV
110 120 130
CDAWGRLPVD LAEEQGHRDI ARYLHAATGD
Length:130
Mass (Da):13,749
Last modified:October 1, 1996 - v1
Checksum:iAC45B21FA69FAD92
GO
Isoform Short (identifier: P55272-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: No experimental confirmation available.

Show »
Length:85
Mass (Da):9,174
Checksum:i18B7A5B6D45C86EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4545Missing in isoform Short. CuratedVSP_018702Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79760 mRNA. Translation: AAB35360.1.
AF474978 mRNA. Translation: AAL76340.1.
AF474979 mRNA. Translation: AAL76341.1.
S77734 Genomic DNA. No translation available.
RefSeqiNP_570825.1. NM_130812.3. [P55272-1]
UniGeneiRn.105626.

Genome annotation databases

EnsembliENSRNOT00000008898; ENSRNOP00000008898; ENSRNOG00000006735. [P55272-1]
GeneIDi25164.
KEGGirno:25164.
UCSCiRGD:2324. rat. [P55272-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79760 mRNA. Translation: AAB35360.1.
AF474978 mRNA. Translation: AAL76340.1.
AF474979 mRNA. Translation: AAL76341.1.
S77734 Genomic DNA. No translation available.
RefSeqiNP_570825.1. NM_130812.3. [P55272-1]
UniGeneiRn.105626.

3D structure databases

ProteinModelPortaliP55272.
SMRiP55272. Positions 1-130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008898.

Proteomic databases

PaxDbiP55272.
PRIDEiP55272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008898; ENSRNOP00000008898; ENSRNOG00000006735. [P55272-1]
GeneIDi25164.
KEGGirno:25164.
UCSCiRGD:2324. rat. [P55272-1]

Organism-specific databases

CTDi1030.
RGDi2324. Cdkn2b.

Phylogenomic databases

eggNOGiNOG287192.
GeneTreeiENSGT00390000004527.
HOGENOMiHOG000290191.
HOVERGENiHBG050870.
InParanoidiP55272.
KOiK04685.
OMAiVMMMGSV.
OrthoDBiEOG7TTQ94.
PhylomeDBiP55272.
TreeFamiTF352389.

Enzyme and pathway databases

ReactomeiREACT_194748. Senescence-Associated Secretory Phenotype (SASP).
REACT_203770. Oxidative Stress Induced Senescence.
REACT_215142. Oncogene Induced Senescence.
REACT_253353. Cyclin D associated events in G1.

Miscellaneous databases

NextBioi605637.
PROiP55272.

Gene expression databases

GenevestigatoriP55272.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular genetic basis of renal carcinogenesis in the Eker rat model of tuberous sclerosis (Tsc2)."
    Hino O., Kobayashi E., Hirayama Y., Kobayashi T., Kubo Y., Tsuchiya H., Kikuchi Y., Mitani H.
    Mol. Carcinog. 14:23-27(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Buckles L.K., Shull J.D.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ACI/SegHsd and COP.
    Tissue: Lung.
  3. "Association of rat p15INK4B/p16INK4 deletions with monosomy 5 in kidney epithelial cell lines but not primary renal tumors."
    Knapek D.F., Serrano M., Beach D., Trono D., Walker C.L.
    Cancer Res. 55:1607-1612(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-86.

Entry informationi

Entry nameiCDN2B_RAT
AccessioniPrimary (citable) accession number: P55272
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.